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Immunostimulatory effects of anionic alkali mineral complex solution Barodon in porcine lymphocytes
Byung Woo Yoo, Soo Il Choi, So Hyun Kim, Soo Jin Yang, Hye Cheong Koo, Sang Hoon Seo, Bong Kyun Park, Han Sang Yoo, Yong Ho Park 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 3 15-24 (10 pages)
K ions as an alkali (pH 13.5) solution. Although Barodon was patented in US as an anionic solu- tion and also registered in Korea, the exact mechanism of Barodon and its effect to host animal is unknown. This study was designed to evaluate Barodon as a nonspecific immunostimulating agent in pigs. A set of monoclonal antibodies specifically reactive with porcine leukocyte dif- ferentiation antigens... The anionic alkali mineral complex solution, Barodon (Barodon-S.F. Corp., Korea), was evaluated for its effectiveness as a nonspecific immunostimulator in pigs. The effects of Barodon were determined by analysis of feed efficiency, growth rate, and phenotype of leukocyte subpopulations using monoclonal antibodies specific to porcine leukocyte differentiation antigens and flow cytometry (FC). The study was focused to investigate the change in proportion of the CD4+CD8+ double positive T... -
Adsorption of cationic dye (MB) and anionic dye (AG 25) by physically and chemically activated carbons developed from rice husk
A.M.Youssef, A. I. Ahmed , U. A. El-Bana 한국탄소학회 Carbon Letters 12 Pages
한국탄소학회 Carbon Letters 2012, Vol.13 No.2 1 61-72 (12 pages)
..PAGE:1 61 Adsorption of cationic dye (MB) and anionic dye (AG 25) by physically and chemically activated carbons developed from rice husk A.M.Youssef ♠, A. I. Ahmed and U. A. El-Bana Department of Chemistry, Faculty of Science, Mansoura University, Mansoura, Egypt Received 25 January 2012 Accepted 2 April 2012 *Corresponding Author E-mail: amayosef@mans.edu.eg Open Access pISSN: 1976-4251... -
Efficacy evaluation of commercial disinfectants by using Salmonella enterica serovar Typhimurium as a test organism
Yangho Jang, Kwangjick Lee, Seonjong Yun, Myoungheon Lee, Jaeyoung Song, Byungjoon Chang, Nong-hoon Choe 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 11 209-216 (8 pages)
temperature in the work area in order to achieve complete decontamination of S. Typhimurium. The activity of CA is enhanced by the presence of anionic detergents like QACs. Therefore, combination agents can be applicable in different fields, including processing industries, after establishing the appropriate concentrations at low temperatures or by expanding the exposure times [12]. At 0 o C... The efficacies of six commercial disinfectants were evaluated by using Salmonella enterica serovar Typhimurium under simulated natural conditions such as sub-zero temperature, short disinfecting time, and surface type (uneven or smooth). We used a suspensionmodel test to determine the disinfecting efficacy under varying contact times (1, 5, 10, and 30 min) and temperatures (25°C, 4°C, 0°C, and -10°C). The bactericidal effect according to surface structure was measured by using a carriermodel... -
Increased humoral antibody response of foot-and-mouth disease virus vaccine in growing pigs pre-treated with poly-γ-glutamic acid
Jee-Hoon Lee, Ik-Jae Kang, A-Reum Kim, You-Sun Noh, Hee-Chun Chung, Bong-Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 15 253-256 (4 pages)
biodegradable and unusual anionic homopolyamide. -PGA is a versatile material that has been widely applied owing to its unique properties [4]. However, efficacy of this substance needs to be elucidated as an immune modulator of FMDV vaccine adjuvant with double oil-based emulsions. In Korea, a single dose immunization of trivalent FMDV vaccine has been preferably adopted for 8 to 12 week old... This study was conducted to determine if humoral antibody response of foot-and-mouth disease (FMD) vaccine improved in 8-week-old growing pigs born to well-vaccinated sows pre-treated with 60 mg of poly-γ-glutamic acid (γ-PGA) three days before vaccination. Antibody against FMD virus serotype O was measured 0, 2, 4 and 6 weeks post-vaccination, using a PrioCHECK FMDV type O ELISA kit. The results showed that positive antibody reactions against FMDV serotype O antigen among a component of the... -
Genistein attenuates isoflurane-induced neurotoxicity and improves impaired spatial learning and memory by regulating cAMP/CREB and BDNF-TrkB-PI3K/Akt signaling
TaoJiang, Xiu-qinWang, ChuanDing, Xue-lianDu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.6 2 579-589 (11 pages)
1A). A significant increase (p<0.05) in the TUNEL-positive cell count was observed in rat pups exposed to isoflurane alone. Staining with FJB, an anionic X fluorescein derivative that specifically stains degenerating neurons, also revealed severe neuronal apoptosis in the hippocampal CA1, CA3, and dentate gyrus regions (Fig. 1B), and increased numbers of FJB-positive neurons were seen in the... Anesthetics are used extensively in surgeries and related procedures to prevent pain. However, there is some concern regarding neuronal degeneration and cognitive deficits arising from regular anesthetic exposure. Recent studies have indicated that brain-derived neurotrophic factor (BDNF) and cyclic AMP response element-binding protein (CREB) are involved in learning and memory processes. Genistein, a plant-derived isoflavone, has been shown to exhibit neuroprotective effects. The present study... -
The Critical Roles of Zinc: Beyond Impact on Myocardial Signaling
SungRyulLee, SuJinNoh, JuliusRyanPronto, YuJeongJeong, HyoungKyuKim, InSungSong, ZhelongXu, HyogYoungKwon, SeChanKang, Eun-HwaSohn, KyungS 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.5 1 389-400 (12 pages)
such as in ische- mia [38,39], from ER and Golgi through ZnTs, mitochondria with unknown mechanisms [40], or acidic organelles [41]. The partial cationic character of Zn 2+ enables it to attract anionic prooxidants, such as peroxynitrite (ONOO ). For example, zinc is released from endothelial nitric oxide syn- thase (eNOS) upon exposure to peroxynitrite, which results in dimer disruption and... Zinc has been considered as a vital constituent of proteins, including enzymes. Mobile reactive zinc (Zn2+) is the key form of zinc involved in signal transductions, which are mainly driven by its binding to proteins or the release of zinc from proteins, possibly via a redox switch. There has been growing evidence of zinc’s critical role in cell signaling, due to its flexible coordination geometry and rapid shifts in protein conformation to perform biological reactions. The importance and... -
Comparison of Ectopic Gene Expression Methods in Rat Neural Stem Cells
WoosukKim, JiHyeonKim, Sun-YoungKong, Min-HyePark, UyDongSohn, Hyun-JungKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.1 4 23-30 (8 pages)
on trans- duction efficiency and some studies showed that anionic polymers inhibit the transduction processes [66-68]. How- ever, other studies showed opposite results that retroviral transduction efficiency is enhanced when an anionic poly- mer is added with equal or higher weight concentration of a cationic polymer [69,70]. Recently, several studies have demonstrated that some other cationic... Neural stem cells (NSCs) have the ability to proliferate and differentiate into various types of cells that compose the nervous system. To study functions of genes in stem cell biology, genes or siRNAs need to be transfected. However, it is difficult to transfect ectopic genes into NSCs. Thus to identify the suitable method to achieve high transfection efficiency, we compared lipid transfection, electroporation, nucleofection and retroviral transduction. Among the methods that we tested, we... -
Quercetin-induced Growth Inhibition in Human Bladder Cancer Cells Is Associated with an Increase in Ca2＋-activated K＋ Channels
YangmiKim, Wun-JaeKim, , Eun-JongCha 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.5 5 279-284 (6 pages)
chan- nel antagonist or quercetin, we investigated variations of MFI by FACS analysis using voltage sensitive dye DiBAC 4(3) (100 nM) (Fig. 3). DiBAC4(3), the anionic bis-oxonol dye is known to accumulate in the cytoplasm of depolarized cells [29]. Therefore, in FACS analysis, depolarization causes an increase in MFI or a shift to the right of fluorescence peak. To test whether the dye could... Quercetin (3,3',4',5,7-pentahydroxyflavone) is an attractive therapeutic flavonoid for cancer treatment because of its beneficial properties including apoptotic, antioxidant, and antiproliferative effects on cancer cells. However, the exact mechanism of action of quercetin on ion channel modulation is poorly understood in bladder cancer 253J cells. In this study, we demonstrated that large conductance Ca2＋- activated K＋ (BKCa) or MaxiK channels were functionally expressed in 253J cells, and... -
Effects of Chlorhexidine digluconate on Rotational Rate of n-(9-Anthroyloxy)stearic acid in Model Membranes of Total Lipids Extracted from Porphyromonas gingivalis Outer Membranes
Hye-OckJang, Dong-WonKim, Byeong-IllKim, Hong-GuSim, Young-HoLee, Jong-HwaLee, Jung-HaBae, Moon-KyoungBae, Tae-HyukKwon, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.2 3 83-88 (6 pages)
& Quintana (1975) have demonstrated that ion-ion interactions take place between the dicationic chlorhexidine molecules and anionic carboxylate groups in stearic acid monolayers at pH of 5.0 ∼6.0 (but not at pH 3), and that the hexamethylene hydrophobic chain of the biguanide is constrained at the cell surface. They further demonstrated the ability of chlorhexidine to anchor to the polar... The purpose of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Large unilamellar vesicles (OPGTL) were prepared with total lipids extracted from cultured Porphyromonas gingivalis outer membranes (OPG). The anthroyloxy probes were located at a graded series of depths inside a membrane, depending on its substitution position (n) in the aliphatic chain. Fluorescence polarization of n-(9-anthroyloxy)stearic acid was used... -
Effects of Chlorhexidine Digluconate on Rotational Rate of n-(9-Anthroyloxy)stearic Acid in Porphyromonas ginginvalis Outer Membranes
Hye-OckJang, Seong-KweonCha, ChangLee, Min-GakChoi, Sung-RyulHuh, Sang-HunShin, In-KyoChung, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 2 125-130 (6 pages)
& Quintana (1975) have demonstrated that ion-ion inte ractions take place between the dicationic chlorhexidine molecules and anionic carb- oxylate groups in stearic acid monolayers at pH of 5.0 ∼6.0 (but not at pH 3), and that the hexamethylene hydrophobic chain of the biguanide is constrained at the cell surface. They further demonstrated the ability of chlorhexidine to anchor to the polar... The aim of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Fluorescence polarization of n-(9-anthroyloxy)stearic acid was used to examine the effect of chlorhexidine digluconate on differential rotational mobility of different positions of the number of membrane bilayer phospholipid carbon atoms. The six membrane components differed with respect to 2, 3, 6, 9, 12, and 16-(9-anthroyloxy)stearic acid (2-AS, 3-AS, 6-AS,...