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Synergistic Interactions of Schizostatin Identified from Schizophyllum commune with Demethylation Inhibitor Fungicides
Min Young Park, Byeong Jun Jeon, Ji Eun Kang, Beom Seok Kim 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 5 579-590 (12 pages)
was performed with 39 cycles of 95°C for 15 s, 58°C for 15 s, and 72°C for 15 s. The threshold cycle values were obtained by the CFX Manager Software (version 3.1). Expression level was normalized using the levels of the housekeeping genes actin (BCIN_16g02020) and ubiquitin (BCIN_11g04470). qPCR assay was conducted with three technical replicates and two biological replicates. The... with 5 μg/ml tebuconazole, schizostatin (1 μg/ml) showed disease control efficacy against gray mold on tomato leaf similar to that achieved with 20 μg/ml tebuconazole treatment alone. Interestingly, schizostatin showed demethylation inhibitor (DMI)-specific synergistic interactions in the crossed-paper strip assay using commercial fungicides. In a checkerboard assay with schizostatin and DMIs, the fractional inhibitory concentration values were 0.0938-0.375. To assess the molecular mechanisms... -
Biological Control of Rice Bakanae by an Endophytic Bacillus oryzicola YC7007
한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 7 228-241 (14 pages)
PCR in leaves inoculated with only pathogen (left), and combination of B. oryzicola YC7007 and pathogen (right). Transcript accumulation levels were normalized using OsActin1 as an internal reference and expressed relative to the normalized expression levels in control and inoculated plants at 0, 6, 12, 24, 48, and 72 hours after inoculation. OsPRI-a OsLOX-L2 OsAOC OsActin1 Mock YC7007 0... In our previous study, we reported that a novel endophytic bacterium Bacillus oryzicola YC7007 has suppressed bacterial diseases of rice via induced systemic resistance and antibiotic production. This endophytic strain, B. oryzicola YC7007 was used as a biological control agent against bakanae disease of rice caused by Fusarium fujikuroi, and its mechanism of interaction with the pathogen and the rice was further elucidated. Root drenching with B. oryzicola YC7007 suspension reduced the disease... -
A Short-chain Dehydrogenase/reductase Gene is Required for Infection-related Development and Pathogenicity in Magnaporthe oryzae
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 2 8-16 (9 pages)
RT-PCT and SDR1-Q for qRT-PCR) are listed in Table 1. To compare the relative abundance of target gene transcripts, the average threshold cycle (Ct) was normalized to that of β- tubulin for the samples as follows: 2 ΔCt, where ΔCt = (Ct, target geneCt,β-tubulin). The fold-change for a target gene during fungal development, as compared to its growth in liquid complete medium, was calculated as... The phytopathogenic fungus Magnaporthe oryzae is a major limiting factor in rice production. To understand the genetic basis of M. oryzae pathogenic development, we previously analyzed a library of T-DNA insertional mutants of M. oryzae, and identified ATMT0879A1 as one of the pathogenicity-defective mutants. Molecular analyses and database searches revealed that a single TDNA insertion in ATMT0879A1 resulted in functional interference with an annotated gene, MGG00056, which encodes a... -
Induction of Drought Stress Resistance by Multi-Functional PGPR Bacillus Licheniformis K11 in Pepper
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 2호 10 201-208 (8 pages)
teins were detected by alkaline silver staining. Quanti- fication of protein spots was carried out using the PDQuest™ software (Bio-Rad, Hercules, CA), and the quantity of protein in each spot was normalized against total valid spot intensity. For protein identification, protein spots were ..PAGE:3 Induced Drought Stress Tolerance by PGPR 203 excised, digested with trypsin (Promega, Madison, WI),... Drought stress is one of the major yield affecting factor for pepper plant. The effects of PGPRs were analyzed in relation with drought resistance. The PGPRs inoculated pepper plants tolerate the drought stress and survived as compared to non-inoculated pepper plants that died after 15 days of drought stress. Variations in protein and RNA accumulation patterns of inoculated and noninoculated pepper plants subjected to drought conditions for 10 days were confirmed by two dimensional...