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Ritanserin, a 5HT2/1C Receptor Antagonist, Does Not Block Cocaine- Induced Behavioral Alterations and zif268 mRNA Expression in the Striatum of the Rats
EunSangChoeJongYeonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.5 3 355-359 (5 pages)
Note that ritanserin did not block cocaine-indllced zif68 mRNA cXIπ SsiOll in thc two rcgions. Vch, ‘ chi c1c; ‘al, sa1inc; coc, c ιainc; rIl, ritansc-n. *p、0.05as compared with vehicle plus saline control group expreSSlOn as 、;eenin the CPu DISCUSSION In dus smdy‘ RIT did not dccrcasc bchaviora1 alteration‘ and zif268 mRNA expression induced by an acule injecLion o[ cocaine... alterations induced by cocaine were also monitored in relation with blockade of the receptors. Systemic injection of ritanserin (1 mg/kg, s.c.), a 5HT2/1C receptor antagonist, did not reverse behavioral alterations and zif268 mRNA gene expression induced by 15 mg/kg cocaine, i.p., in the dorsal and ventral striatum. These data indicate that ritanserin-sensitive 5HT2/1C receptors are not necessary for cocaine-induced behavioral alterations and zif268 mRNA gene expression in the striatum. -
Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers
Huayuan Lin, Qiqi Huang, Xiaoquan Guo, Ping Liu, Weilian Liu, Yuelong Zou, Shuliang Zhu, Guangfu Deng, Jun Kuang, Caiying Zhang, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 5 423-429 (7 pages)
properly cited. pISSN 1229-845X eISSN 1976-555X Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers Huayuan Lin †, Qiqi Huang †, Xiaoquan Guo*, Ping Liu*, Weilian Liu, Yuelong Zou, Shuliang Zhu, Guangfu Deng, Jun Kuang, Caiying Zhang, Huabin Cao, Guoliang Hu Clinical Veterinary Laboratory, College of Animal... 8 and 15 dpi (p < 0.01), while serum malondialdehyde concentrations were significantly higher (p < 0.01). The serum uric acid was significantly higher than that of the control group at 15 dpi (p < 0.01). In addition, the kidney mRNA transcript level and serum activity of XOD in the infected group was significantly higher than that of the control group at 8, 15 and 22 dpi (p < 0.05). The results indicated that NIBV infection could cause the increases of renal XOD gene transcription and serum... -
Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development
Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)
stable level. [Ca 2+ ]i did not differ among the IVF 72 h, 120 h and 140 h groups (p > ..PAGE:5 Dynamic analysis of Ca 2+ level in oocytes during early embryonic development 137 Fig. 5. Ca 2+ distribution in bovine parthenogenetic activation (PA) embryos at different times. 0.01). Additionally, although the [Ca 2+ ]i differed among the 14 h, 24 h and 160 h groups [Ca 2+ ]i, this difference was... Mammalian oocyte maturation and early embryo development processes are Ca-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca was distributed in the cortical ooplasm and throughout the oocytes from... -
Block of hERG K+ Channel by Classic Histamine H1 Receptor Antagonist Chlorpheniramine
Hee-KyungHong, Su-HyunJo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.3 12 215-220 (6 pages)
0 mV (left panel), +20 mV (middle panel) and +40 mV (right panel), before and after expo- sure to 20 μM chlorpheniramine, showing increased block of hERG current at more positive membrane potentials. The protocol consisted o f 4 s depolarizing steps to 0 mV, +20 mV or +40 mV from a holding potential of 70 mV, followed by repolarization to 60 mV. (B) Con- centration-dependent block of I... current amplitude at the end of the voltage steps and hERG tail currents. The IC50 of chlorpheniramine-dependent hERG block in Xenopus oocytes decreased progressively relative to the degree of depolarization. Chlorpheniramine affected the channels in the activated and inactivated states but not in the closed states. The S6 domain mutations Y652A and F656A partially attenuated (Y652A) or abolished (F656A) the hERG current block. These results suggest that the H1 antihistamine, chlorpheniramine is... -
Direct Block of Cloned K+ Channels, Kv1.5 and Kv1.3, by Cyclosporin A, Independent of Calcineurin Inhibition
BokHeeChoi, SangJuneHahn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.6 7 353-362 (10 pages)
of 15.5±2.7 ms (n=5) and 27.5±4.2 ms (n= 5), respectively. Those two values were significantly different (p< 0.05). The dotted line represents zero level of current. Data are expressed as mean±s.e. Fig. 8. Use-dependent block of Kv1.5 current by CsA. (A) Fifteen repetitive 125 ms depolarizing pulses of +50 mV from a holdin g potential of -80 mV were applied at two different frequencies, 1 and... with a steep increase over the voltage range of channel opening. However, the block exhibited voltage independence over the voltage range in which channels were fully activated. The rate constants for association and dissociation of CsA were 7.0μM1s1 and 8.1 s1, respectively. CsA slowed the deactivation time course, resulting in a tail crossover phenomenon. Block of Kv1.5 by CsA was use-dependent. CsA also blocked Kv1.3 currents at 50 mV in a reversible... -
Block of ATP-Sensitive K Channels Expressed in Xenopus Oocytes by Dimethyl Sulfoxide
JinBongParkSooWanChae 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.2 6 157-163 (7 pages)
Block of ATP-Sensitive K - Channels Expressed in Xenopus Oocytes by Dimethyl Sulfoxide Jin Bong Park1 and SOO Wan Chae Department of Phannacology and Institute of Cardiovascular Research, Chonbuk National University Medical School, Jeonju 560-182, Korea The effects of dimethyl sulfoxide (DMSO) were studied in two groups of Xenopus oocytes, one expressing ATP sensitive K+ (KATP) channel... The effects of dimethyl sulfoxide (DMSO) were studied in two groups of Xenopus oocytes, one expressing ATP sensitive K (KATP) channel comprised of sulfonylurea receptor SUR1 and inwardly rectifying K channel subunit Kir6.2, and the other expressing renal KATP channel ROMK2. At concentrations of 0.3∼10% (vol/vol) DMSO inhibited whole cell Kir6.2/SUR1 currents elicited by bath application of sodium azide (3 mM) in a concentration-dependent manner. The inhibition constant and Hill coefficient... -
Conduction Block of the Primary Afferent Fibers by Topically Applied Allyl Isotheocyanate
Shin, Hong-Kee, Kim, Kee-Soon, 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 2 123-132 (10 pages)
by mustard oil. Our findings suggest that the desensitization of the afferent fibers and sensory receptors induced by mustard oil results mainly from the conduction block of C-fibers. ..PAGE:9 -Ally! Isotheocyanate-induced Conduction Block- 131 REFERENCES Brain SD, Williams TJ, Tippins JR, Morris HR & Macintyre I 1985) Calcitonin gene-related peptide is a... ound action potentials recorded from rat tibial nerve before and after topical application of mustard oil were compared. The higher the concentration of mustard oil and the longer the application time, the more powerful desensitization or conduction block was induced. From the results of the present study, it is suggested that the desensitization of the afferent fiber and sensory receptors induced by mustard oil results mainly from the conduction block of C-fiber in the primary afferent nerve. -
NecroX-5 exerts anti-inflammatory and anti-fibrotic effects via modulation of the TNFα/Dcn/TGFβ1/Smad2 pathway in hypoxia/reoxygenation-treated rat hearts
VuThiThu, HyoungKyuKim, LeThanhLong, ToThanhThuy, NguyenQuangHuy, SoonHaKim, NariKim, KyungSooKo, ByoungDooRhee, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.3 10 305-314 (10 pages)
injury [31] and inhibited sodium nitroprusside- induced cardiac cell death [29]. Furthermore, proteomic analysis revealed that NecroX-5 preserved the cellular level of several anti- inflammatory proteins in hypoxia/reoxygenation (HR)-treated hearts. As an extension of this previous study, we investigated whether NecroX-5 plays a role in modulating inflammatory and fibrotic responses in an... Inflammatory and fibrotic responses are accelerated during the reperfusion period, and excessive fibrosis and inflammation contribute to cardiac malfunction. NecroX compounds have been shown to protect the liver and heart from ischemia-reperfusion injury. The aim of this study was to further define the role and mechanism of action of NecroX-5 in regulating infl ammation and fi brosis responses in a model of hypoxia/reoxygenation (HR). We utilized HR-treated rat hearts and lipopolysaccharide... -
Ca2+ is a Regulator of the WNK/OSR1/NKCC Pathway in a Human Salivary Gland Cell Line
SoonhongPark, SangKyunKu, HyeWonJi, Jong-HoonChoi, DongMinShin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.3 9 249-255 (7 pages)
to block the influx of Ca 2+. 2APB also inhibits the IP 3 induced Ca 2+ increase. However, even though 2APB acts as an inhibitor of either IP 3 induced Ca 2+ increase or nonselec tive cation channel, the outcome of 2APB treatment is an inhibition of [Ca 2+]i increase. In the results pretreatment with 2APB inhibited the elevation of the [Ca 2+ ]i and reduced the level of p-OSR1 induced by... in the WNK-OSR1-NKCC pathway, such as Wnk1, Wnk4, OSR1, SPAK, and NKCC1. The HSG cell line showed an intracellular Ca2+ concentration ([Ca2+]i) increase in response to hypotonic stimulation, and the response was synchronized with the phosphorylation of OSR1. Interestingly, when we inhibited the hypotonically induced [Ca2+]i increase with nonspecific Ca2+ channel blockers such as 2-aminoethoxydiphenyl borate, gadolinium, and lanthanum, the phosphorylated OSR1 level was also diminished. Moreover,... -
Effects on G2/M Phase Cell Cycle Distribution and Aneuploidy Formation of Exposure to a 60 Hz Electromagnetic Field in Combination with Ionizing Radiation or Hydrogen Peroxide in L132 Nontumorigenic Human Lung Epithelial Cells
HeeJin, HyeEunYoon, Jae-SeonLee, Jae-KyungKim, SungHoMyung, Yun-SilLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.2 6 119-124 (6 pages)
1 mT ELF-MF) in combination with IR significantly in- creased the level of γ-H2AX. In contrast, exposure to a 2 mT ELF-MF in combination with H 2O2 did not sig- nificantly alter the level of γ-H2AX [23]. We did not ob- serve any alterations in G2/M phase arrest, aneuploidy pat- terns, or cell death upon ex posure to a 2 mT ELF-MF in the present study. Therefore, further studies of the effects... The aim of the present study was to assess whether exposure to the combination of an extremely low frequency magnetic field (ELF-MF; 60 Hz, 1 mT or 2 mT) with a stress factor, such as ionizing radiation (IR) or H2O2, results in genomic instability in non-tumorigenic human lung epithelial L132 cells. To this end, the percentages of G2/M-arrested cells and aneuploid cells were examined. Exposure to 0.5 Gy IR or 0.05 mM H2O2 for 9 h resulted in the highest levels of aneuploidy; however, no cells...


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