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본문검색 결과만 보기
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Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
Lee. Hye-Ja, Park. Mi-Kyung, Park. Young-Ran, Kim. Dong-Hak, Yun. Chul-Ho, Chun. Young-Jin, Shin. Hee-Jung, Na. Han-Sung, Chung. Myeon-W 한국독성학회 Toxicological research 5 Pages
한국독성학회 Toxicological research 2011, Vol.27 No.1 25-29 (5 pages)
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Merkel cells in the follicle-sinus complexes of muzzle skins are useful for postmortem diagnosis of rabid dogs
Chun-ho Park, Taichi Shimatsu, Kazunori Kimitsuki, Nozomi Shiwa, DariaL. Manalo, Satoshi Inoue 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 24 94-95 (2 pages)
cytokine at0,4,7 days post infection (dpi). From two-week old healthy ducks, spleen andblood from heart were collected for splenic mononuclear cell (SMC) andperipheral blood mononuclear cell (PBMC) stimulation by killed R. anatipestifer serovar 7. Sp-MQ wereisolated from two-week old healthy ducks and transfected by siRNA and then stimulatedwith supernatants of COS-7 cells transfected with... -
Common Cytokine Receptorgamma chain mediates IL-17A expression against Riemerella anatipestifer
Fahmida Afrin, Cherry Fernandez, Rochelle Flores, Woo Kim, Jipseol Jeong, Hyun Lillehoj, Suk Kim, Wongi Min 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 23 94-94 (1 pages)
cytokine at0,4,7 days post infection (dpi). From two-week old healthy ducks, spleen andblood from heart were collected for splenic mononuclear cell (SMC) andperipheral blood mononuclear cell (PBMC) stimulation by killed R. anatipestifer serovar 7. Sp-MQ wereisolated from two-week old healthy ducks and transfected by siRNA and then stimulatedwith supernatants of COS-7 cells transfected with... -
Determination of aflatoxin B1, deoxynivalenol, fumonisin B1, ochratoxin A, zearalenone in cow stomach content by LC-MS/MS
Kwang Nam Kim, Soo-hee Kim, Hyo-bi Kim, Jae Young Song, Sung Won Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 22 93-94 (2 pages)
cytokine at0,4,7 days post infection (dpi). From two-week old healthy ducks, spleen andblood from heart were collected for splenic mononuclear cell (SMC) andperipheral blood mononuclear cell (PBMC) stimulation by killed R. anatipestifer serovar 7. Sp-MQ wereisolated from two-week old healthy ducks and transfected by siRNA and then stimulatedwith supernatants of COS-7 cells transfected with... -
Suppression of common cytokine receptor γ chain by siRNA inhibits IL-17A expression in R. anatipestifer-stimulated cells
Wongi Min, Fahmida Afrin, Cherry Fernez, Rochelle Flores, Woo Kim, Jipseol Jeong, Yeong H. Song, Hyun Lillehoj, Suk Kim 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 55 232-232 (1 pages)
IL-17A, IL-17F, IL-6 and IL-1 β markedly increased in splenic lymphocytesand macrophages stimulated with killed R. anatipestifer comparedto unstimulated and cultured controls. COS-7 cells transientlywere transfected a FLAG-tagged du γ c-a or du γ c-bconstruct. Both forms were present in the cell lysatewhereas only du γc-b from the culturesupernatant was detected. All siRNA sequences... -
Single-Channel Recording of TASK-3-like K+ Channel and Up- Regulation of TASK-3 mRNA Expression after Spinal Cord Injury in Rat Dorsal Root Ganglion Neurons
InseokJang, Jun-HoLa, Gyu-TaeKim, Jeong-SoonLee, Eun-JinKim, Eun-ShinLee, Su-JeongKim, Jeong-MinSeo, Sang-HoAhn, Jae-YongPark, Seong-GeunH 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.5 5 245-252 (8 pages)
chan- nels at different membrane potentials in cell-attached patches. The mean open time and the current-voltage rela- tionship of this K + channel, determined from duration and amplitude histograms, were similar to those of cloned TASK-3 expressed in COS-7 cells. In DRG neurons, TASK- 3-like K + channel activity was decr eased after the formation of inside-out patches but did not change over... in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K+ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like K+ channel was 33.0±0.1 pS at −60 mV, and TASK-3 activity fell by 65±5% when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG K+ channel is similar to... -
The Inhibition of TREK2 Channel by an Oxidizing Agent, 5,5'-dithio- bis (2-nitrobenzoic acid), via Interaction with the C-terminus Distal to the 353rd Amino Acid
KyoungSunPark, HyoweonBang, Eun-YoungShin, ChanHyungKim, YangmiKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.4 13 211-215 (5 pages)
TREK2. The pipette and bath solu- tion contained 150 mM KCl, 5 mM EGTA, 10 mM HEPES, and 1 mM MgCl 2. The asterisk indicates a significant difference from the respective control (p＜0.05). METHODS Cell culture and transfection COS-7 cells were plated on 35 mm culture dishes one day before transfection. Rat TREK2 and its mutant forms were cloned into the pcDNA3.1 vect or. The plasmids were then... COS-7 cells, and the channel activities were recorded from inside-out membrane patches using holding potential of −40 mV in symmetrical 150 mM K＋ solution. Intra</SUP>cellular application of an oxidizing agent, 5,5'-dithio-bis (2-nitrobenzoic acid) (DTNB), markedly decreased the activity of the TREK2, and the activity was partially reversed by the reducing agent, dithiothreitol (DTT). In order to examine the possibility that the target sites for the oxidizing agents might be located... -
Prostaglandin E1 Increases cGMP Levels in Beating Rabbit Atria: Lack of Effects of PGE1-induced Cyclic Nucleotides on Secretory and Contractile Functions
XuanShunJin, HeXiuQuan, SunYoungKim, SungHunPark, SungZooKim, HoSubLee, KyungWooCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2007, Vol.11 No.5 3 175-182 (8 pages)
concentrations of NO; higher NO concentration generated lower cGMP levels in GC isozyme expressing COS-7 cells. Rapid desensitization of NO receptor sGC has been suggested to be a possible cause of the bell-shaped concentration-response curve for NO in intact cells (Bel- lamy et al, 2000; Gibb et al, 2003). In increasing levels of cyclic nucleotides in the beating rabbit cardiac atrium, PGE 1... Members of prostaglandin (PG) E-series elicit cellular effects mainly through adenylyl cyclase-cAMP signaling. The role of PGE2-induced increase in cAMP has been shown to be compartmentalized in the cardiac myocytes: PGE2-induced increase of cAMP is not involved in the control of cardiomyocytic contraction. The purpose of the present study was to define the effect of PGE1 on the cGMP levels and the role of PGE1 in the atrial secretory function. Experiments were performed in perfused beating... -
Differential Activation of Ras/Raf/MAPK Pathway between Heart and Cerebral Artery in Isoproterenol-induced Cardiac Hypertrophy
HyunjuKim, NariKim, HyunJoo, JaeBoumYoum, WonSunPark, MohamedWarda, SunghyunKang, VuThiThu, TranMinhKhoa, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.5 7 299-304 (6 pages)
a variety of cell types such as Rat-1 cells, smooth muscle cells, Chinese hamster ovary cells, COS-7, and adipocytes (Burgering et al, 1993; Cook et al, 1993; Wu et al, 1993; VanRenterghem et al, 1994; D 'Angelo et al, 1997; Hecquet et al, 2002). On the contrary, however, in some cell types such as PC12 cells, Swiss-3T3 cells, and S49 mouse lymphoma cells, cAMP activates ERKs and potentates... Cardiac hypertrophy contributes an increased risk to major cerebrovascular events. However, the molecular mechanisms underlying cerebrovascular dysfunction during cardiac hypertrophy have not yet been characterized. In the present study, we examined the molecular mechanism of isoproterenol (ISO)-evoked activation of Ras/Raf/MAPK pathways as well as PKA activity in cerebral artery of rabbits, and we also studied whether the activations of these signaling pathways were altered in cerebral artery,... -
주의력결핍 과잉행동장애에서 도파민 운반체 유전자의 10-Repeat Allele Homozygosity와 [123I]IPT SPECT로 측정한 도파민 운반체 밀도가 Methylphenidate 치료 반응에 미치는 영향
천근아, 유영훈, 김재원 대한신경정신의학회 신경정신의학 8 Pages
대한신경정신의학회 신경정신의학 2004, 제 43권 제 2호 4 151-158 (8 pages)
7 또는 9- repeat allele보다 10-repeat allele를 더 많이 함유할 경우 COS-7 세포에서의 luciferase reporter system에 서의 luciferase 표현이 증가됨으로써 DAT1의 유전자 표현이 영향을 받을 가능성을 제시한 Fuke 등 15)의 in vitro 연구결과와도 일치하는 것이다. 또한, 본 연구의 결 과는 in vivo 연구인 Heinz 등 25)이 [ 123 I]β-CIT SPECT 를 이용하여 수행한 DAT 밀도와 DAT1의 VNTR 다형 성 간의 연관성 연구와도...