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A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
Sang Kyu Park, Sangho Roh*, Jong-Im Park* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 8 73-80 (8 pages)
in RNA processing [29]. The length of an mRNA poly(A) tail is crucial for stabilization of the 3’ end of the mRNA transcript. Thus, the expression of PolyA is important for mammalian embryonic development [29,30]. Expression of GLUT-1 that encodes the metabolic protein GLUT-1 is significantly decreased in morulae produced in vitro compared to those generated in vivo [31]. The heat shock... Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated with demecolcine that facilitated enucleation with protruding genetic material. Enucleation and donor... -
Application of biotechnological tools for coccidia vaccine development
Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 1 279-288 (10 pages)
a post-hybridization image is scanned to capture fluorescence images using a ScanArray 4000 Microarray Analysis System (GSL Lumonics) and is analyzed using ScanAlyze software developed at Stanford University. The color density of individual nucleic acid species reflects the relative amount of labeled cDNA hybridized to the DNA immobilized at the known position of the array. By comparing samples... Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and... -
Comparison of Interleukin-8 Levels in Long-Distance Runners and Healthy Sedentary Non-Athletic Control Subjects
Young-OhShin, Jun-SangBae, Ji-WonMin, Jeong-BeomLee, Jung-KyuKim, Young-JuSong, Hun-MoYang, Young-KiMin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2007, Vol.11 No.6 6 263-268 (6 pages)
of rowing. Med Sci Sports Exerc 32: 1384 －1389, 2000 Modi WS, Chen ZQ. Localization of the human CXC chemokine subfamily on the long arm of chromosome 4 using radiation hybrids. Genomics 47: 136 －139, 1998 Modi WS, Dean M, Matsushima K, Seuanez H, O'Brien SJ. Chromosome mapping and RFLP analyses of monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8). (Abstract) Cytogenet Cell Genet... We have previously demonstrated that the level of leukocytes and neutrophils significantly increased immediately and 30 min after exercise. Interleukin-8 (IL-8) is an inflammatory cytokine that acts as a chemokine on neutrophils. In the present study, we evaluated the correlation between the number of neutrophils and leukocytes, and between the number of neutrophils and plasma IL-8 level. Long-distance trained runners (TRs, n = 10) and untrained sedentary control subjects (SEDs, n = 10) ran for... -
정신분열병과 Glutathione S-transferase P1 유전자 다형성
배치운, 이영민, 양대현, 김정진, 유혜숙, 이창욱, 이수정, 이철, 백인호 대한신경정신의학회 신경정신의학 6 Pages
대한신경정신의학회 신경정신의학 2004, 제 43권 제 1호 5 23-28 (6 pages)
map of eight human chromosome 11q markers, including DRD2, spanning 60 cM. Cytogenet Cell Genet 1992;60:26-28. 15) Hu X, Xia H, Srivastava SK, Pal A, Awasthi YC, Zimniak P, et al. Catalytic efficiencies of allelic variants of human glutathione S-trans- ferase P1-1 toward carcinogenic anti-diol epoxides of benzo[c] phenanthrene and benzo[g]chrysene. Cancer Res 1998;58:5340-5343. 16) Harada S,... -
Comparative Genome Analysis of Rathayibacter tritici NCPPB 1953 with Rathayibacter toxicus Strains Can Facilitate Studies on Mechanisms of Nematode Association and Host Infection
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 4호 3 370-381 (12 pages)
1 0.03 RNA processing and modification K 196 6.84 Transcription L 126 4.40 Replication, recombination, and repair B 0 0.00 Chromatin structure and dynamics D 35 1.22 Cell cycle control, cell division, chromosome partitioning V 68 2.37 Defense mechanisms T 113 3.94 Signal transduction mechanisms M 149 5.20 Cell wall/membrane biogenesis N 16 0.56 Cell motility U 20 0.70 Intracellular trafficking and... of one circular chromosome of 3,354,681 bp with a GC content of 69.48%. A total of 2,979 genes were predicted, comprising 2,866 protein coding genes and 49 RNA genes. The comparative genomic analyses between R. tritici NCPPB 1953 and R. toxicus strains identified 1,052 specific genes in R. tritici NCPPB 1953. Using the BlastKOALA database, we revealed that the flexible genome of R. tritici NCPPB 1953 is highly enriched in Environmental Information Processing system and metabolic processes for... -
국산 Fluorescence in Situ Hybridization 시스템을 이용한 다양한 검체에서의 염색체 분석
문신용, 방명걸, 오선경, 류범용, 황도영, 정병준, 최진, 손철, 장준근, 김종원, 김석현, 최영민, Moon. Shin-Yong, Pang. Myung-Geol, Oh. Sun-Kyung, Ryu. Buom-Yong, Hwang. Do-Yeong, Jung 대한생식의학회 大韓不妊學會雜誌 6 Pages
대한생식의학회 大韓不妊學會雜誌 1997, Vol.24 No.3 335-340 (6 pages)
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Suppression of Autophagy and Activation of Glycogen Synthase Kinase 3beta Facilitate the Aggregate Formation of Tau
Song-InKim, Won-KiLee, Sang-SooKang, Sue-YoungLee, Myeong-JaJeong, HeeJaeLee, Sung-SooKim, GallV.W.Johnson, WanjooChun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.2 6 107-114 (8 pages)
107 Korean J Physiol Pharmacol Vol 15: 107 －114, April, 2011 DOI: 10.4196/kjpp.2011.15.2.107 ABBREVIATIONS: AD, Alzheimer's disease; NFT, neurofibrillary tangle; FTDP-17, parkinsonism linked to chromosome 17; PHFs, paired helical filaments; GSK3 β, glycogen synthase kinase 3 β. Received March 25, 2011, Revised April 17, 2011, Accepted April 20, 2011 Corresponding to: Wanjoo Chun, Department... Neurofibrillary tangle (NFT) is a characteristic hallmark of Alzheimer's disease. GSK3Ղ has been reported to play a major role in the NFT formation of tau. Dysfunction of autophagy might facilitate the aggregate formation of tau. The present study examined the role of GSK3Ղ-mediated phosphorylation of tau species on their autophagic degradation. We transfected wild type tau (T4), caspase-3-cleaved tau at Asp421 (T4C3), or pseudophosphorylated tau at Ser396/Ser404 (T4-2EC) in the...