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Conflict effect of poly-gamma-glutamic acid on inflammation
Cheon Ho Kim, Geun Shik Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 99 138-139 (2 pages)
optosis detection kit or propidium iodide stain, respectively. The expression levels of proteins related with apoptosis and cell cycle arrest were analyzed by western blot. Results: The viability of PC-3 cells was decreased in dose-dependent manner by NSE-EtOH. The rates of cell population induced apoptosis and cell cycle arrest were increased as compared with control. In the expressions of... -
An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus
Ji Eun Yu, In-Ohk Ouh, Hyeonjeong Kang, Hye-young Lee, Kwang-Myun Cheong, In-Soo Cho, Sang-Ho Cha 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 7 519-527 (9 pages)
infection than current diagnostic methods makes it highly useful for minimizing the economic impact of a disease outbreak. Despite the availability of commercial ICST for PRRSV-specific antibody detection, diagnostic performance of commercially available ICSTs in the field has not been well described. The aim of this study was to develop a rapid and sensitive ICST based on type 1 and type 2... study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus (PRRSV)-specific antibodies. The method utilized colloidal gold nanoparticle-labeled dual-type nucleocapsid proteins encoded by open reading frame 7. We evaluated 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs. Based on true PRRSV-specific antibody-positive or -negative sera determined by immunofluorescence assay and IgM enzyme-linked... -
Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 4 350-357 (8 pages)
detection system of microbes for bovine (Dembo) diarrheal diseases via real-time polymerase chain reaction (Dembo diarrhea-PCR) and a similar system for bovine respiratory disease complex via real-time PCR (Dembo respiratory-PCR), respectively; both detection systems are based on TaqMan real-time PCR [13,31]. The Dembo-PCR method exhibits high sensitivity, high specificity, rapidity, and a... Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain... -
Detection of hepatitis E virus genotypes 3 and 4 in pig farms in Korea
Yong-Hyun Kim, Byung-Joo Park, Hee-Seop Ahn, Sang-Hoon Han, Hyeon-Jeong Go, Dong-Hwi Kim, Joong-Bok Lee, Seung-Yong Park, Chang- 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 18 309-312 (4 pages)
properly cited. pISSN 1229-845X eISSN 1976-555X Detection of hepatitis E virus genotypes 3 and 4 in pig farms in Korea Yong-Hyun Kim, Byung-Joo Park, Hee-Seop Ahn, Sang-Hoon Han, Hyeon-Jeong Go, Dong-Hwi Kim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, In-Soo Choi* Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea Zoonotic... Zoonotic transmission of hepatitis E virus (HEV) is mostly mediated by HEV-3 and HEV-4 genotypes, and domestic pigs are an important reservoir of these genotypes. A survey of 14 pig farms in Korea revealed HEV RNA in 30 of 148 (20.3%) fecal samples. HEV-3a and HEV-4c subtypes were identified in five pig farms (35.7%) and two pig farms (14.3%), respectively. Phylogenetic analysis indicated that the isolated HEV strains were closely related to previously reported zoonotic strains in Korea. The... -
Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick
Guimin Zhao, Hongmei Wang, Peili Hou, Chengqiang He, Hongbin He 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 10 242-250 (9 pages)
novel molecular technology for rapid, low-resource use diagnostics. It includes three kinds of detection methods for testing the DNA amplification result [14]. In addition, an RPA-LFD combination is more beneficial for clinical point- of-care diagnoses. At present, RPA assays have been established and reported for the rapid detection of several pathogens [1,11,18]. Moreover, a real-time RPA... a detection limit of up to eight copies per reaction, which was equivalent to that of the real-time quantitative polymerase chain reaction (qPCR) assay. The assay was specific, as it did not amplify genomes from five other Mycobacterium spp. or five pathogenic enteric bacteria. Six hundred-twelve clinical samples (320 fecal and 292 serum) were assessed by RPA-LFD, qPCR, and enzyme-linked immunosorbent assay, respectively. The RPA-LFD assay yielded 100% sensitivity, 97.63% specificity, and 98.44%... -
Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 7 307-316 (10 pages)
antigen, the commercial IDEXX ELISA kit (IDEXX Laboratories, USA) has been widely used for the detection of PRRSV antibodies. Recently, an ELISA kit that uses recombinant Nsp7 protein as the antigen has been introduced, and the concurrence rate between that kit and the IDEXX ELISA assay has been reported to be up to 97.6% [2]. Although traditional laboratory tests offer good sensitivity and... a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with... -
Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Hye Kwon Kim, Kwang Soo Lyoo, Thi My Le Huynh, Hyoung Joon Moon, Van Giap Nguyen, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 16 253-256 (4 pages)
lots (lanes 13), PCV1-free PK15 cells (lane 4), PCV2-infected PK15 cells (lane 5), type 2 PRRSV (lane 6), PRRSV and PCV2 co-infected tissue (lane 7), and MARC-145 cells (lane 8). Fig. 2. Detection limits of the duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method. A mixture of 10 5 porcine circovirus type 2 (PCV2)-infected PK15 cells/mL and 10 5.5 TCID50/mL type 2... infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex nested RT-PCR were 10 TCID50/mL for type 2 PRRSV and 10 infected cells/mL for PCV2. The kappa statistic, which measures agreement between methods, was 0.867, indicating a good level of agreement. This RNA-based duplex RT-PCR approach can be another way to detect type 2 PRRSV and PCV2 simultaneously and with improved convenience. -
Estrogen receptor-α, progesterone receptor, and c-erbB/HER-family receptor mRNA detection and phenotype analysis in spontaneous canine models of breast cancer
Farruk M. Lutful Kabir, Patricia DeInnocentes, Payal Agarwal, Christopher P. Mill, David J. Riese 2nd, R. Curtis Bird 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 4 149-158 (10 pages)
and c- erb B/HER-family receptor mRNA detection and phenotype analysis in spontaneous canine models of breast cancer Farruk M. Lutful Kabir 1,†, Patricia DeInnocentes 1, Payal Agarwal 2, Christopher P. Mill 3,‡, David J. Riese 2nd 3, R. Curtis Bird 1,* Auburn University Research Initiative in Cancer (AURIC), 1 Department of Pathobiology, 2 Scott-Ritchey Research Center, College of Veterinary... Well characterized, stable, p16-defective canine mammary cancer (CMT) cell lines and normal canine mammary epithelial cells were used to investigate expression of the major breast cancer-specific hormone receptors estrogen receptor alpha (ER1) and progesterone receptor (PR) as well as luminal epithelial-specific proto-oncogenes encoding c-erbB-1 (epidermal growth factor receptor/EGFr), c-erbB-2/HER2, c-erbB-3, and c-erbB-4 receptors. The investigation developed and validated quantitative reverse... -
First detection of West Nile virus in domestic pigeon in Korea
C-Yoon Kim, Hanseul Oh, Juha Song, Moonsuk Hur, Jae-Hwa Suh, Weon-Hwa Jheong, Jong-Taek Kim, Hong-Shik Oh, Jae-Hak Park 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 20 587-589 (3 pages)
WNV using African green monkey kidney-derived Vero cells and repeated the entire process of detection without the positive sample. Based on the results, we could rule out the possibility of JEV infection. Although, we could not detect any WNV sequences other than the E region of WNV, the results clearly demonstrated the presence of WNV in Vero cells and supernatant based on the specific E... West Nile virus (WNV) is a mosquito-borne zoonotic pathogen that has spread throughout Europe and the United States. Recently, WNV spread to East and Southeast Asia, and great efforts have been made in South Korea to prevent the spread of WNV from neighboring countries. In this study, we diagnosed the first case of WNV in pigeons (Columba livia domestica) residing in cities using a competitive enzyme-linked immunosorbent assay and confirmed it with nested reverse transcription polymerase chain...