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Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer
Anukul Taweechaipaisankul, Geon A Kim, Jun-Xue Jin, Su Cheong Yeom, Byeong Chun Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 3 492-499 (8 pages)
cross bred (Korean native black pig and landrace) pigs with different coat colors. Asian-Australas J Anim Sci 2013, 26, 1047-1053. 5. Hwang IS, Kwon DJ, Oh KB, Ock SA, Chung HJ, Cho IC, Lee JW, Im GS, Hwang S. Production of cloned Korean native pig by somatic cell nuclear transfer. Dev Reprod 2015, 19, 79-84. 6. Kim GA, Lee EM, Jin JX, Lee S, Taweechaipaisankul A, Hwang JI, Alam Z, Ahn C,... establish and evaluate blood type O cells derived from Korean native pig (KNP), a typical miniature pig breed in Korea. Ten cell lines derived from 8 KNP piglets and one adult female KNP (kidney and ear tissues) were established. To confirm the presence of blood type O, genomic DNA, fucosyltransferase (FUT) expression, and immunofluorescence staining were examined. Additionally, fluorescence-activated cell sorting and somatic cell nuclear transfer were performed to investigate the normality of... -
Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips
Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 8 159-166 (8 pages)
no cross-reac- tivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specifcity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to de- tect BYDV in oat samples from six different... a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42oC for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus,... -
Influence fo Pyrolysis Cindition and type of Rasin on the Porosity of Activated Carbon Obtained From Phenolic Resins
Damyanti Agarwel, Darashan Lal, V.S.Tripathi, G.N.Mathur 한국탄소학회 Carbon Letters 7 Pages
한국탄소학회 Carbon Letters 2003, Vol.4 No.2 1 57-63 (7 pages)
85% phenol novolac type of resin gives better char yield than phenol resol resin, however, highest char yield ie. 67.5% was obtained with phenol resol resin showing 98% curing. This indicates that both physical and chemical structures of resin significantly affect the char yield and the resins prepared with ammonia as catalyst show better curing. As catalyst and cross-linking agent both have amine... -
The use of laser Doppler blood flow to assess the effect of acute administration of vitamin D on micro vascular endothelial function in people with diabetes
Jerrold Petrofsky, Faris Alshammari, Iman Akef Khowailed, Riya Lodha, Pooja Deshpande, Praveen Rajaram, Mahendra Gaikwad, Vidhi Vadera 물리치료재활과학회 Physical Therapy Rehabilitation Science 7 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2013, 제 2권 제 2호 1 63-69 (7 pages)
D administration on the skin blood flow response to occlusion and heat. Design: Cross-sectional study. Methods: Twenty age matched subjects; 10 who had diabetes and 10 who were controls were administered 4,000 IU of vitamin D3 for 3 weeks at breakfast. The function of the endothelial cells was evaluated in 2 ways; first, the response to 4 minutes of vas- cular occlusion of the skin was measured... administration on the skin blood flow response to occlusion and heat. Design: Cross-sectional study. Methods: Twenty age matched subjects; 10 who had diabetes and 10 who were controls were administered 4,000 IU of vitamin D3 for 3 weeks at breakfast. The function of the endothelial cells was evaluated in 2 ways; first, the response to 4 minutes of vascular occlusion of the skin was measured with a laser Doppler flow meter. Second, the skin blood flow response to local heat at 42 degrees C for 6... -
Control of type O foot-and-mouth disease by vaccination in Korea, 2014-2015
Jong-Hyeon Park, Dongseob Tark, Kwang-Nyeong Lee, Ji-Eun Chun, Hyang-Sim Lee, Young-Joon Ko, Soo-Jeong Kye, Yong-Joo Kim, Jae-Ku 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 13 271-279 (9 pages)
division between the viral titers (serum titer against viruses used for cross virus neutralization test/serum titer against vaccine viruses in the vaccination groups). † Tested by World Reference Laboratory for FMD (Pirbright Institute), UK except an indication note. ‡ Tested by MSD Animal Health (FMD vaccine manufacturer). §Tested by Merial Boehringer Ingelheim (FMD vaccine manufacturer).... type O foot-and-mouth disease (FMD) outbreak began in Korea. Although vaccinations were administered, FMD cases increased steadily for five months, and reached 185 cases by April 2015. Most of the affected animals were pigs, which are vulnerable to vaccination. The FMD virus belonged to the South-East Asia (SEA) topotype that had been observed three times in Korea between April 2010 and July 2014. However, the FMD virus isolated in December 2014 had a unique feature; that is, partial deletion of... -
Botulinum toxin type A enhances the inhibitory spontaneous postsynaptic currents on the substantia gelatinosa neurons of the subnucleus caudalis in immature mice
Seon-Hui Jang, Soo-Joung Park, Chang-Jin Lee, Dong-Kuk Ahn, Seong-Kyu Han 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 7 539-546 (8 pages)
for a cross-modality mechanism. J Neurosci. 2003;23:7719-7725. 38. Ruiz-Medina J, Baulies A, Bura SA, Valverde O. Paclitaxel-induced neuropathic pain is age dependent and devolves on glial response. Eur J Pain. 2013;17:75-85. 39. Park SA, Yang EJ, Han SK, Park SJ. Age-related changes in the ef- fects of 5-hydroxytryptamine on substantia gelatinosa neurons of the trigeminal subnucleus caudalis.... Botulinum toxin type A (BoNT/A) has been used therapeutically for various conditions including dystonia, cerebral palsy, wrinkle, hyperhidrosis and pain control. The substantia gelatinosa (SG) neurons of the trigeminal subnucleus caudalis (Vc) receive orofacial nociceptive information from primary afferents and transmit the information to higher brain center. Although many studies have shown the analgesic effects of BoNT/A, the effects of BoNT/A at the central nervous system and the action... -
The Effects of Consciousness-raising Tasks on EFL Learners Grammar Acquisition and Flow
Ma Jee Hyun, Cho Young Ah 이화여자대학교 교과교육연구소 교과교육학연구 10 Pages
이화여자대학교 교과교육연구소 교과교육학연구 2019, 제 23권 1호 2 16-25 (10 pages)
(1996) suggested seven techniques within the task-based approach: identification, classification, hypothesis build- ing/checking, cross-language exploration, reconstruction/ deconstruction, recall, and reference training. Gimeno (2003) stated that there are three levels of CR activities. First, examples relevant to a problematic structure are given, then the rules are discovered, and finally, an... The current study examines the effects of consciousness-raising tasks on L2 learners grammar competence and their flow experiences. The participants consisted of 77 Korean college students who were assigned to two consciousness-raising task groups the hypothesis building/checking and classification and one control group. The study used a background questionnaire, pre-, post-, and delayed English grammar tests, and also pre-, and post-flow perceptions questionnaires. The results indicate that... -
Flow cytometric immunophenotyping of canine adipose-derived mesenchymal stem cells (ADMSCs) and feline ADMSCs using anti-human antibodies
Minho Ko , Kwon Young Lee , Sae Hoon Kim , Manho Kim , Jung Hoon Choi , Wooseok Im , Jin Young Chung 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2018, 제 58권 제 1호 5 33-37 (5 pages)
one type. To test the cross-reactivity, six monoclonal antibod- ies against various CD molecules were tested in each ADMSC. Flow cytometric analysis of dog ADMSCs revealed that the cells had cross-reactivity 0.73% with CD44 and 3.74% with CD133, while it was 0.15%, 0.1%, 0.07% and 0.38% for CD31, CD34, CD42, and CD62, respectively (Fig. 3). Flow cytometric analysis of cat ADMSCs revealed that the... -
Mechanical Properties of C-type Mesophase Carbon Fibers
Seung Kon Ryu, Bo Sung Rhee, Xiao Ping Yang and Yafei Lu 한국탄소학회 Carbon Letters 5 Pages
한국탄소학회 Carbon Letters Vol.1 No.3&4 9 165-169 (5 pages)
C-type fibers were not so much changed during oxida- tion and carbonization. In order to produce equal thickness fibers, the round fibers had to be extruded at a lower mass flow rate. Thus, the round fibers were melt-spun at a higher Fig. 1. Schematic view of pitch-spinning apparatus. Fig. 2. Design and size of C-type spinnerette. Table 1. Properties of naphtha cracking bottom oil and precur- sor... -
Nitric Oxide Synthase Mediates Carbon Monoxide-Induced Stimulation of L-type Calcium Currents in Human Jejunal Smooth Muscle Cells
InjaLim, JihyunYun, SeungtaeKim, SoonchulMyung, TaehoKim, HyoweonBang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.3 7 161-166 (6 pages)
μl of 1 % CO solution was gently added to the bath (500 μl) to 0.2% final concentration. CO solution was applied directly by syringe into bath solution to prevent mechanoactivation of L-type Ca 2+ channel by continuous flow. Fig. 1. The effect of exogenous CO on L-type Ca 2+ currents of human jejunal circular smooth muscle cells. -100 -80 -60 -40 -20 20 40 120 100 80 60 40 20 CONTROL CO... Exogenous carbon monoxide (0.2%) increases L-type calcium (Ca2) current in human jejunal circular smooth muscle cells. The stimulatory effect of carbon monoxide (CO) on L-type Ca2 current is inhibited by pre-application of L-NNA, a classical competitive inhibitor of nitric oxide synthase (NOS) with no significant isoform selectivity (Lim, 2003). In the present study, we investigated which isoform of NOS affected CO induced stimulation of L-type Ca2 current in human jejunal...


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