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Optical Resolution of Racemic α-Hydroxycarboxylic Acids on a Dynamic Chiral Stationary Phase Derived from (S)-Leucinol by Ligand Exchange Chromatography
Hyun. Myung-Ho, Kim. Jung-In, Cho. Yoon-Jae, Ryoo. Jae-Jeong 대한화학회 Bulletin of the Korean Chemical Society 4 Pages
대한화학회 Bulletin of the Korean Chemical Society 2004, Vol.25 No.11 1707-1710 (4 pages)
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Marek’s disease vaccine activates chicken macrophages
Dan Wang, Shuhong Sun, Mohammad Heidari 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 7 375-383 (9 pages)
the manufacturer’s recommendations. MicroBeads coated with goat anti-mouse IgG1 (Miltenyi Biotec, USA) were used for separation of macrophages from the mixed splenocytes according to the manufacturer’s recommendations. An LS column and a MACS Separator (Miltenyi Biotec) were used to collect macrophages ..PAGE:3 Immune responses to Marek’s disease vaccine 377 www.vetsci.org Table 1. Primer... To provide insights into the role of innate immune responses in vaccine-mediated protection, we investigated the effect of Marek’s disease (MD) vaccine, CVI988/Rispens, on the expression patterns of selected genes associated with activation of macrophages in MD-resistant and MD-susceptible chicken lines. Upregulation of interferon , interleukin (IL)-1, IL-8, and IL-12 at different days post-inoculation (dpi) revealed activation of macrophages in both chicken lines. A strong... -
Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
a reverse phase column. Mobile phase A contained 0.1% formic acid in MilliQ water and mobile phase B consisted of 0.1% formic acid in acetonitrile. Approximately 20 g of the trypsinized peptide mixture was loaded onto the column. Desalting and separation were initiated using 3% buffer B for 5 min, 4% to 50% buffer B for 90 min, 50% to 90% buffer B for 10 min, and 98% buffer A for 10 min at a... Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel... -
Kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats
Mengyun Li, Wuqing Ouyang*, Xiaoli Wu, Yin Zheng, Yunpeng Wei, Lei An 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 3 353-359 (7 pages)
a solution with 0.06 mol/L. Enzyme- linked immunosorbent assay (ELISA) kits specific for IL-2 and IL-6 were obtained from Xinbosheng Biotechnology (China). Lymphocyte separation medium was purchased from Shanghai Huajing Biological Technology (China). A ΔΨm detection kit (JC-1), annexin V-FITC apoptosis detection kit, and cell cycle detection kit were obtained from BiYunTian (China). A... Kinetin (Kn) is a cytokinin growth factor that exerts several anti-aging and antioxidant effects on cells and organs. To investigate the mechanism underlying apoptotic events in aging cells induced by D-galactose (D-gal), we examined the effect of Kn delivered via nuchal subcutaneous injection on D-gal-induced aging and apoptosis in rats. Our results showed that interleukin (IL)-2 levels and mitochondrial membrane potential (ΔΨm) were decreased by Kn in aging rats while IL-6 production and... -
Development of in vitro produced porcine embryos according to serum types as macromolecule
Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 11 315-321 (7 pages)
by the least significant difference mean separation procedure when treatments differed at p ＜ 0.05. Cell number was expressed as the mean ± SEM. Results Comparison of development rates of porcine embryos according to origins of PS without GTH Maturation rates of porcine oocytes after using different stage PS without GTH for supplementation of maturation media are shown in Table 1. The... This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient porcine serum during porcine in vitro production. To investigate the efficient porcine serum (PS), different types of PS [newborn pig serum, prepubertal gilt serum (PGS), estrus sow serum, and pregnancy sow serum] were used to supplement IVM media with or without gonadotrophin (GTH) and development rates of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were then... -
Physiology,Pharmacology,Chemistry : Comparison of Linear and non-Linear equation for the calibration of roxithromycin analysis using Liquid chromatography/mass spectrometry
Jong Hwan Lim , Hyo In Yun 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2010, 제 50권 제 1호 2 11-17 (7 pages)
purchased from Sigma (USA). Whole blood was obtained from healthy male beagle dogs. Instruments and chromatographic conditions Samples were analyzed on a Hewlett-Packard 1100 series LC/MSD system. Separation was achieved on Watchers120 ODS-BP C 18 reverse phase column (5m, 4.6 mm × 150 mm; Daiso, Japan). Mobile phase composed of 20% of 10 mM ammonium acetate (pH 3.5) and 80% of acetonitrile. Th... -
Effects of Fluvastatin on the Pharmacokinetics of Repaglinide: Possible Role of CYP3A4 and P-glycoprotein Inhibition by Fluvastatin
Chong-KiLee, Jun-ShikChoi, JoonSeokBang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.3 10 245-251 (7 pages)
C. The dried extract was reconstituted with 150 μl of mobile phase, vortex-mixed and transferred to a clean autosampler vial. A 70- μl aliquot of the super- natant was injected into the HPLC system. Chromatogra- phic separation was achieved using a μBondapak TM C18 col- umn (3.9×300 mm i.d., 10 μm, Waters Co.) attached to a μBondapak TM C18 guard column (3.9×20 mm i.d., 10 μm, Waters... The purpose of this study was to investigate the effects of fluvastatin on the pharmacokinetics of repaglinide in rats. The effect of fluvastatin on P-glycoprotein and CYP3A4 activity was evaluated. The pharmacokinetic parameters and blood glucose concentrations were also determined after oral and intravenous administration of repaglinide to rats in the presence and absence of fluvastatin. Fluvastatin inhibited CYP3A4 activity in a concentration-dependent manner with a 50% inhibition... -
Modern Methods for Analysis of Antiepileptic Drugs in the Biological Fluids for Pharmacokinetics, Bioequivalence and Therapeutic Drug Monitoring
JuseopKang, Yoo-SinPark, Shin-HeeKim, Sang-HyunKim, Min-YoungJun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 16 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.2 1 67-82 (16 pages)
and a reversed-phase HPLC assay which involves a diastereo- meric derivative formation with tert-butyloxy-L-leucine N-hydroxy-succinimide ester [239]. The latter, however, does not appear to have been applied to biological matrices. More recently, some results demonstrated that the enantio- selective separation of mixtures of D,L-aminoacids can be accomplished by HPLC after converting each... Epilepsy is a chronic disease occurring in approximately 1.0% of the world's population. About 30% of the epileptic patients treated with availably antiepileptic drugs (AEDs) continue to have seizures and are considered therapy-resistant or refractory patients. The ultimate goal for the use of AEDs is complete cessation of seizures without side effects. Because of a narrow therapeutic index of AEDs, a complete understanding of its clinical pharmacokinetics is essential for understanding of the... -
정신분석의 역사-분석이론의 진화를 중심으로
대한신경정신의학회 신경정신의학 2010, 제 49권 제 1호 5 9-35 (27 pages)
정상 자폐적 단계(Normal Autistic Phase) - 정상 공생 단계(Normal Symbiotic Phase) - 분리-개별화 과정(Separation-Individuation Process) - 분리와 신체상의 발달(Differentiation and Development of Body Image Subphase) - 연습 단계(Practicing Subphase) - 재접근 단계(Rapprochement Subphase) - 개별성의 확립과 대상항상성의 단계(Individuality and Object Constancy Subphase) Spitz는 유아가 모든 심리적 발달을... -
Physiology is Not an Appendage of Anatomy Illustrated with Deterministic Chaos -Annual Review Lecture, October 11, 1991-
Partridge, Lloyd D. 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 1 1-14 (14 pages)
with Deterministic Chaos- 7 While it is still illogical to package neuro- physiology separately from the rest of phy- siology this book meets requirements for cur- rent separation while illustrating the fact that even the neuro parts of function need not be organized on topics such as spinal cord, medulla, pons, cerebellum, etc. Properly the chapters of this book should be distributed within...