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공개키 인증서를 사용하지 않는 전방향 안전성을 보장하는 E-mail 프로토콜
권정옥, 구영주, 정익래, 이동훈, Kwon. Jeong-Ok, Koo. Young-Ju, Jeong. Ik-Rae, Lee. Dong-Hoon 한국정보보호학회 情報保護學會論文誌 9 Pages
한국정보보호학회 情報保護學會論文誌 2009, Vol.19 No.1 3-11 (9 pages)
이메일 시스템에서 전방향 안전성(forward secrecy)이란, 메일 사용자와 메일 서버가 장기간 사용하는 비밀키(long-term key)가 노출되더라도 이전 세션들에서 전송된 이메일 메시지의 기밀성(confidentiality)이 보장되는 것을 말한다. 기존 전방향 안전성을 지원하는 이메일 프로토콜들에서는 사용자의 공개키를 사용하기 때문에 공개키에 대한 인증을 위해서는 공개키 기반구조(PKI, Public Key Infrastructure)의 구축이 반드시 필요하다. 본 논문에서는 PKI의 구축이 필요 없이, 사용자의 메일 계정의 패스워드만을 이용하는 전방향... -
Development qRT-PCR Protocol to Predict Strawberry Fusarium Wilt Occurrence
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 3호 2 163-170 (8 pages)
Plant Pathology The Plant Pathology Journal Research Article Open Access Development qRT-PCR Protocol to Predict Strawberry Fusarium Wilt Occurrence Sung Won Hong 1,†, Da-Ran Kim 2,†, Ji Su Kim 1, Gyeongjun Cho 1, Chang Wook Jeon 1, and Youn-Sig Kwak 1,2* 1Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 52828, Korea 2Department of Plant Medicine, Institute of... of soil was required to cause the disease in both lab and field conditions. With the minimum density to develop the wilt disease, the pathogen affected near 60% in nursery plantation. A biological control microbe agent and soil solarization reduced the pathogen population 2-fold and 1.5-fold in soil, respectively. The developed F. oxysporum f. sp. fragariae specific qRT-PCR protocol may contribute to evaluating soil healthiness and appropriate decision making to control the disease. -
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 4 123-131 (9 pages)
a Real-time PCR Protocol for the Specifc Detec- tion and Quantifcation of Clavibacter michiganensis subsp. sepedonicus in Potato Min Seok Cho 1,3,, Duck Hwan Park 2,, Min Namgung 2, Tae-Young Ahn 3 and Dong Suk Park 1* 1National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-550, Korea 2Department of Applied Biology, College of Agriculture and Life Sciences, Kangwon... Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced... -
Induction of antibody and interferon-γ production in mice immunized with virus-like particles of swine hepatitis E virus
Young-Jo Song, Woo-Jung Park, Seul-Kee Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Kun-Ho Seo, Young-Sun 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 15 575-578 (4 pages)
in the medio-lateral position with forced flexion (ML) and the other in a cranio-caudal position with the animals under general anaesthesia or deep sedation. The current IEWG elbow screening protocol includes submission of these specific quality flexed ML radiographs of both elbows for osteophyte evaluation [18]. Next, we classified the degree of dysplasia according to the IEWG protocol... Virus-like particles (VLPs) composed of the truncated capsid protein of swine hepatitis E virus (HEV) were developed and immune responses of mice immunized with the VLPs were evaluated. IgG titers specific for the capsid protein of swine HEV were significantly higher for all groups of mice immunized with the VLPs than those of the negative control mice. Splenocytes from mice immunized with the VLPs also produced significantly greater quantities of interferon (IFN)-γ than interleukin (IL)-4 and... -
Expression of E-cadherin in pig kidney
Su-Youn Lee, Sun M. Han, Ji-Eun Kim, Ku-Yong Chung, Ki-Hwan Han 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 2 381-386 (6 pages)
techniques as previously described [9,10,13]. For the first immunoperoxidase procedure, the protocol for single labeling was performed as described above. After the diaminobenzidine reaction, the sections were washed in PBS, incubated in 3% H 2O2 for 30 min, and then in protein blocking solution (DakoCytomation, Dako) for 30 min at room temperature. The sections were treated for 1 h with... E-cadherin is a cell adhesion molecule that plays an important role in maintaining renal epithelial polarity and integrity. The purpose of this study was to determine the exact cellular localization of E-cadherin in pig kidney. Kidney tissues from pigs were processed for light and electron microscopy immunocytochemistry, and immunoblot analysis. E-cadhedrin bands of the same size were detected by immunoblot of samples from rat and pig kidneys. In pig kidney, strong E-cadherin expression was... -
Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus
Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 13 467-472 (6 pages)
corresponding to the capsid gene was cloned into the Champion pET-100/ TOPO vector (Invitrogen, USA) in TOP10 competent Escherichia (E.) coli cells (Invitrogen) according to the manufacturer’s protocol. The sequence of the resulting construct was confirmed with automatic-dye-terminator DNA sequencing (ABI Prism 377 L; Applied Biosystems, USA). The cloned plasmid was used to transform BL21... Swine hepatitis E virus (HEV) is widespread throughout pigs in both developing and industrialized countries. This virus is an important zoonotic agent and a public concern worldwide. Infected pigs are asymptomatic, so diagnosing swine HEV relies on detection of the virus or antibodies against the virus. However, several obstacles need to be overcome for effective and practical serological diagnosis. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) that used a purified... -
(E)-3-(3-methoxyphenyl)-1-(2-pyrrolyl)-2-propenone displays suppression of inflammatory responses via inhibition of Src, Syk, and NF-κB
YongKim, EunJeongJeong, In-SookHanLee, Mi-YeonKim, JaeYoulCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.1 12 91-99 (9 pages)
a 24-well plate, according to the manufacturer’s protocol. The cells were used for experiments 24 h after transfection. Luciferase assays were performed using the Luciferase Assay System (Promega), as reported previously [20]. Preparation of cell lysates and nuclear fractions for immunoblotting RAW264.7 or HEK293 cells (5×10 6 cells/ml) were washed three times in cold PBS and lysed in a... (E )-3-(3-methoxyphenyl)-1-(2-pyrrolyl)-2-propenone (MPP) is an aldol condensation product resulting from pyrrole-2-carbaldehyde and m- and p- substituted acetophenones. However, its biological activity has not yet been evaluated. Since it has been reported that some propenone-type compounds display anti-inflammatory activity, we investigated whether MPP could negatively modulate inflammatory responses. To do this, we employed lipopolysaccharide (LPS)-stimulated macrophage-like RAW264.7 cells... -
Treatment Protocol for Cleft Lip and/or Palate Children in Kyushu University Hospital
Akira Suzuki 대한구순구개열학회 대한구순구개열학회지 13 Pages
대한구순구개열학회 대한구순구개열학회지 2012, 제 15권 2호 3 69-81 (13 pages)
. ..PAGE:13 Treatment Protocol for Cleft Lip and/or Palate Children in Kyus hu University Hospital - 81 - 교신 저자 Akira Suzuki Department of Orthodontics, Kyushu University Hospital, Fukuka, Japan Tel : +81-92-6426460 / E-mail : suzuki.dent@kyudai.jp Comparison of orthodontic and orthopedic effects of a modified maxillary protractor between deciduous and early mixed dentitions. Am J Orthod... Our Team Approach consists of following five stages; (1) Peri-natal care until lip repair After ultrasound diagnosis, some obstetricians recommend the mother with CL/P fetus to undergo prenatal counseling in our CLP clinic. On the day the CL/P baby was born, our oral surgeon, nurse, and pedodontist visit the maternity clinic, and take counseling and take impression for a feeding plate. The cheiloplasty is performed in three months old. (2) From lip repair to palatal repair At one year of age,... -
Comparison between two progesterone sources and two oestradiol formulations in a Heatsynch protocol for postpartum cycling dairy cows in pasture
Maarten F.A. Andringa, Frank J.C.M. Van Eerdenburg, Elisa Fernández, Sofía García, Daniel Cavestany* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 7 161-166 (6 pages)
h. A modified Heatsynch protocol [17] was used for oestrus synchronisation (Fig. 1). The cows were divided into four groups; 16 received the intravaginal device and 12 were treated with each of the parenteral progesterone formulations. The number of cows per group was determined according to availability of the drugs. Four cows had to be eliminated from the study before the end of the... To compare an injectable progesterone (MAD-4) with an intravaginal device (IPD), and natural O17 with synthetic oestradiol (OB) in a synchronisation protocol, 51 cows were divided into four groups. Each group was treated with one of the two sources of progesterone and one of the two oestradiol formulations. Oestrus behaviour, follicle diameter, and pregnancy rates were evaluated. Oestrus behaviour (p = 0.902), numbers of cows in oestrus (p = 0.917), follicle diameter (p = 0.416), and pregnancy... -
Vapor phase Mercury Removal by Sulfur Impregnated ACtivated Carbons and Sulfur Impregnation Protocol
Si Hyun Lee, Sun Ying Cha and Yeong Seomg Park 한국탄소학회 Carbon Letters 7 Pages
한국탄소학회 Carbon Letters 2001, Vol.2 No.1 6 37-43 (7 pages)
Sulfur Impregnation Protocol Si Hyun Lee , Sun Young Cha and Yeong Seong Park* Energy and Environment Research Department, Korea Institute of Energy Research, P.O. Box 103, Yusong, Daejeon, 305-600 Korea *Department of Environmental Engineering, Daejeon University, Tonggu, Daejeon 300-716, Korea e-mail:LSH3452@kier.re.kr (Received February 17, 2001; accepted March 29, 2001) Abstract Mercury has...


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