전체 선택해제
총 37건 검색
본문검색 결과만 보기
-
Electron Microscopic Grid를 이용한 초급속동결이 소 난포란의 발달능에 미치는 영향 - I. 소 미성숙난자의 동결에 관한 연구
김은영, 김남형, 이봉경, 윤산현, 박세필, 정길생, 임진호, Kim. E.Y., Kim. N.H., Yi. B.K., Yoon. S.H., Park. S.P., Chung. K.S., Lim. J.H. 대한생식의학회 大韓不妊學會雜誌 6 Pages
대한생식의학회 大韓不妊學會雜誌 1998, Vol.25 No.1 71-76 (6 pages)
소 미성숙난자를 electron microscope (EM) grid와 동해제인 EFS30을 이용하여 초급속 동결하였을 때 정상적인 배 발달의 유도가능성 여부를 조사하고자 실시하였다. 동해제는 30% ethylene glycol, 18% ficoll, 0.5 M sucrose와 10% FBS 등이 PBS에 첨가되어 제작된 EFS30을 사용하였다. 난자 생존의 평가기준으로는 성숙, 수정 및 배발달을 조사하였다. 본 연구에서 얻어진 결과는 다음과 같다. 초급속동결-융해 후, 소 미성숙란의 생존율은 43.2%을 나타내었다. 동결-융해군의 체외 성숙 (84.1%)과 정상 자웅전핵 형성율 (57.5%)은... -
Helicobacter apodemus sp. nov., a new Helicobacter species identified from the gastrointestinal tract of striped field mice in Korea
Woo Jin Jeon, Hee-Jin Dong, Jae Hoon Shin, Il Yong Kim, Hungwui Ho, Seung Hyun Oh, Young Min Yoon, Yang-Kyu Choi, Jun Gyo Suh, K 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 11 475-481 (7 pages)
a carbon-coated grid, after which a small droplet of 4% phosphotungstic acid solution was added. The morphology of the isolates was examined using a Philips CM10 transmission electron microscope (Philips, The Netherlands) at 80 KeV and compared to the morphology of other known Helicobacter species (Table 2). DNA extraction Bacterial colonies on blood agar plates were propagated in Brucella... to cephalothin and nalidixic acid, and weakly positive for oxidase and catalase activity. Electron microscopy revealed that the bacterium has spirally curved rod morphology with singular bipolar nonsheathed flagella. Genotypically, the isolated bacterial strains (YMRC 000215, YMRC 000216, and YMRC 000419) were most closely related to a reference strain of Helicobacter mesocricetorum (97.25%, 97.32%, and 97.03% 16S rRNA sequence similarities, respectively). The 16S rRNA sequences of these strains... -
The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain
Eliana Guedes Stehling, *, Tatiana Amabile Campos, Marcelo Brocchi, Vasco Ariston de Carvalho Azevedo, Wanderley Dias da Silveira 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 10 75-83 (9 pages)
HEp-2 cells (Table 2). Fimbriae expression was detected when this strain was studied under an electron microscope (Fig. 4A). In the one-day-old chicken assay the LD 50 of strain, SEPT 13 was determined to be 4.0 ×10 5 CFU/ml (Table 2). The PCR experiments demonstrated, in this strain only, the presence of fimA, csgA and tsh genes, and was negative for all the other genes as noted in Table... electron microscopy), and had five plasmids of 2.7, 4.7, 43, 56, and 88 MDa. Transposon mutagenesis of strain SEPT13, with transposon TnphoA, resulted in a mutant strain named ST16 that had a LD50 of 1.2 × 1012 CFU/ml. All other biological characteristics of strain ST16 were the same as those detected for strain SEPT13 except for the migration of an 88 MDa plasmid to the 93 MDa position indicating the insertion of the transposon into the 88 MDa plasmid. The 93 MDa plasmid of strain ST16 was... -
Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity
Sang-Hyun Kim, Yong-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 5 119-124 (6 pages)
surface and/ or the clumps were gravitated to the round bottom of the tubes. Electron microscopy of curli fimbriae Negative staining of CR+ and CR- EHEC bacteria were prepared for EM (Philips, XL30S FESEM, Netherlands) observation. Bacteria grown overnight in LB both were concentrated and resuspended in PBS. The bacterial suspension was mixed with equal volume of 2% (v/v) phosphotungstic acid and... C. A few CR-binding negative (CR-) colonies occurred spontaneously within the population of CR+ isolate. The CR+ EHEC showed massive aggregative adhesion pattern, whereas the spontaneous CR- strain showed typical localized adherence on HEp-2 cells. Electron microscopy confirmed highly curliated bacteria in the CR+ EHEC sample. Interestingly, the curliation disappeared in a msbB1 and msbB2 double mutant derived from the CR+ EHEC. These results suggest that the compromised outer membrane integrity... -
A study on the immunocytochemical localization of eurofascin in rat sciatic nerve
Byung-joon Chang, Ik-hyun Cho and Peter J. Brophy 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 1 67-71 (5 pages)
Comp Neurol, 1991, 310, 234-252 18. Small J. R., Ghabriel M. N., and Allt G. The development of Schmidt-Lanterman incisures: an electron microscope study. J Anat, 1987, 150, 277-286 19. Tait S., Gunn-More F., Collinson J. M., Huang J., Lubetzki C., Pedraza L., Sherman D. L., Colman D. R., and Brophy P. J. An oligodendrocyte cell adhesion molecule at the site of assembly of the paranodal axo-glial... We examined the localization of neurofascin (NF) in the sciatic nerve of rat. In the myelinated fibers, neurofascin localizes strongly in the nodal axolemma except the small central cleft and also expresses in the paranodes, and weakly in the Schmidt-Lanterman incisures. In the paranodes, NF localizes around the axolemma and it expresses in the apposing membrane of paranodal loops. Axoplasm, compact myelin and cytoplasm of Schwann cell do not express NF at all. In the Schmidt-Lanterman... -
Microbiology,Immunology : Characterization of Yersinia ruckeri isolated from the farm-cultured eel Anguilla japonica in Korea
Seong Joon Joh , Chang Hee Kweon , Min Jeong Kim , Min Su Kang , Hwan Jang , Jun Hun Kwon 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2010, 제 50권 제 1호 5 29-35 (7 pages)
forma- coated, 300-mesh grid and allowed to dry. The grid was stained negatively with 2% uranyl acetate (pH 6.5) for 30 sec. The grid was dried at room temperature and then examined by transmission electron microscope (TEM; Hitachi, Japan). Biochemical and enzymatic characterization of the isolates Biochemical characterization was carried out using API 20E strips (BioMerieux, Spain) supplemented... -
랫드 궁둥신경에서 말이집틈새의 형성과 Neurofascin 발현 연관에 대한 면역세포화학적연구
최혜영, 조익현, 이종환, 남상섭, 장병준 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2009, 제 49권 제 4호 1 273-278 (6 pages)
peripheral neuropathy. Glia 1998, 24, 8-20. 20. Small JR, Ghabriel MN, Allt G. The development of Schmidt-Lanterman incisures: an electron microscope study. J Anat 1987, 150, 277-286. 21. Trapp BD, Andrews SB, Wong A, O'Connell M, Griffin JW. Co-localization of the myelin-associated glycoprotein and the microfilament components, F-actin and spectrin, in Schwann cells of myelinated nerve fibres. J... -
Immunocytochemical Study on the Translocation Mechanism of Glucose Transporters by Insulin
Hah, Jong-Sik, Kim, Ku-Ja 대한생리학회 대한생리학회지 16 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 2 123-138 (16 pages)
of gold particles through electron microscope was observed. Our data clearly indicate that the location of the glucose transporter in subcellular fractions could be detected by the immunogold labelling method (Okami et al, 1989). According to this technique our data showed that the distribution pattern of glucose transporters between the cell surface and the intracellular pool (LDM) was... The mechanism of insulin action to increase glucose transport is attributed to glucose transporter translocation from intracellular storage pools to the plasma membrane in insulin-sensitive cells. The present study was designed to visualize the redistribution of the glucose transporter by means of an immunogold labelling method. Our data clearly show that glucose transporter molecules were visible by this method. According to the method this distribution of glucose transporters between cell... -
Control of Basal Stem Rot Disease in Oil Palm by Supplementation of Calcium, Copper, and Salicylic Acid
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 3 396-406 (11 pages)
grid and dried out using flter paper. Then, they were stained with uranyl acetate for 15 min and washed with double distilled water and stained with lead for 10 min, washed with double dis- tilled water again and fnally viewed under transmission electron microscope (TEM). Determination of plant vigour. The effects of Ca and Cu and SA treatments on plant vigour were assessed for every month.... of Ca/Cu/SA was found to be the most effective in controlling the disease and the high performance liquid chromatography results showed the detection of ergosterol at very low concentration in the treated samples. Moreover, the transmission electron microscopy analysis results clearly indicated that T7 treatment was also enhancing lignification, which was responsible for the thickness of the secondary cell walls and middle lamella compared to untreated samples. It was therefore, concluded that... -
Evaluation of Resistance to Ralstonia solanacearum in Tomato Genetic Resources at Seedling Stage
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 1호 8 58-64 (7 pages)
JPN(1), MEX(4), NLD(1), PAN(1), PER(26), USA(8) 1Country codes (ISO 3166-1 alpha-3) ..PAGE:3 Kim et al. 60 tion software. Ultrathin sections (approximately 80 nm thick) were mounted onto copper grid, followed by double-staining with 2% (w/v) uranyl acetate and Reynolds lead citrate for 7 min. Sections were examined under a transmission electron microscope (LEO912AB, Carl Zeiss, Germany) at an... Bacterial wilt of tomatoes caused by Ralstonia solanacearum is a devastating disease that limits the production of tomato in Korea. The best way to control this disease is using genetically resistant tomato plant. The resistance degree to R. solanacearum was evaluated for 285 tomato accessions conserved in the National Agrobiodiversity Center of Rural Development Administration. These accessions of tomato were originated from 23 countries. Disease severity of tomato accessions was investigated...