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Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system
Wonyou Lee, Kilyoung Song, Inhyung Lee, Hyungdo Shin, Byeong Chun Lee, Seongchan Yeon, Goo Jang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 15 509-516 (8 pages)
first polar body; Dg, oocytes with degenerated cytoplasm; Fr, oocytes with fragmented cytoplasm; Sr, oocytes with shrunken cytoplasm after extrusion of polar body. Biosystems) and an ABI 3730 Genetic Analyzer (Applied Biosystems). Sequence variants were verified by means of chromatograms using the DNA star software. Experimental design In experiment 1, the type of needle used for TVUFA in mares... Transvaginal ultrasound-guided follicle aspiration is one method of obtaining recipient oocytes for equine somatic cell nuclear transfer (SCNT). This study was conducted: (1) to evaluate the possibility of oocyte aspiration from pre-ovulatory follicles using a short disposable needle system (14-G) by comparing the oocyte recovery rate with that of a long double lumen needle (12-G); (2) to investigate the developmental competence of recovered oocytes after SCNT and embryo transfer. The recovery... -
Betulinic acid prevents alcohol-induced liver damage by improving the antioxidant system in mice
Jine Yi*, Wei Xia, Jianping Wu, Liyun Yuan, Jing Wu, Di Tu, Jun Fang, Zhuliang Tan* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 16 141-148 (8 pages)
week before the experiment was initiated, and fed an M02 standard mouse diet from Hunan Silaikejingda Laboratory Animal (China). The mice had access to food and water ad libitum, and were maintained at a constant temperature (23 ± 1 o C) and humidity (60 ± 10%) with a 12-h light/dark cycle. The present study complied with the Animal Care and Use Guidelines of China and was approved by the... Betulinic acid (BA), a pentacyclic lupane-type triterpene, has a wide range of bioactivities. The main objective of this work was to evaluate the hepatoprotective activity of BA and the potential mechanism underlying the ability of this compound to prevent liver damage induced by alcohol in vivo. Mice were given oral doses of BA (0.25, 0.5, and 1.0 mg/kg) daily for 14 days, and induced liver injury by feeding 50% alcohol orally at the dosage of 10 ml/kg after 1 h last administration of BA. BA... -
Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
Jung-Taek Kang, Dae-Kee Kwon, Sol-Ji Park, Su-Jin Kim, Joon-Ho Moon, Ok-Jae Koo, Goo Jang, Byeong-Chun Lee* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 3 15-20 (6 pages)
of quercetin during the entire 44-h maturation period. For experiment 2, we evaluated the effects of the same four concentrations of quercetin in IVM medium on the parthenogenetic development of embryos. In experiment 3, we assessed the effects these concentrations of quercetin in IVM medium on the ROS levels in oocytes to assess the effect of antioxidant of quercetin. Reagents Unless... Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte nuclear maturation and embryonic development after parthenogenetic activation. We then evaluated the antioxidant activities of quercetin by measuring reactive oxygen species (ROS) levels in matured oocytes. Immature oocytes were untreated or treated with 1, 10, and 50 ug/mL quercetin during in vitro... -
Influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (Bubalus bubalis) preantral follicles
Pawan K. Dubey, Vrajesh Tripathi, Ram Pratap Singh, G. Taru Sharma* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 9 257-265 (9 pages)
(10 3 M) was excluded from this experiment because it almost abolished PF growth and survival; 10 7 SNP had an inhibitory effect and 10 9 SNP had a stimulatory effect. PFs selected for in vitro culturing were placed in 50 μL (4∼5 PFs/droplet of culture medium) of the respective culture medium in 35 mm plastic tissue culture dishes (Nunc, Denmark) and overlaid with 2 mL of embryo-tested... Effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on in vitro survival, growth, steroidogenesis, and apoptosis of buffalo preantral follicles (PFs) was investigated. PFs (200∼250 mm) were isolated by micro-dissection and cultured in 0 (control), 10, 10, 10, and 10 M SNP. To examine the reversible effect of SNP, PFs were cultured with 10 M SNP ＋ 1 mM N-nitro-L-arginine methyl ester (L-NAME) or 1.0 mg hemoglobin (Hb). The results showed that greater concentrations of SNP (10,... -
Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development
Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)
of experiment and statistical analysis Experiment 1: Investigation of the distribution and fluorescent intensity of Ca 2+ in oocytes of the germinal vesicle (GV), GV breakdown (GVBD), MI, MII phase. Experiment 2: Investigation of the distribution and fluorescent intensity of Ca 2+ in IVF embryos at different ..PAGE:3 Dynamic analysis of Ca 2+ level in oocytes during early embryonic development... Mammalian oocyte maturation and early embryo development processes are Ca-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca was distributed in the cortical ooplasm and throughout the oocytes from... -
In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes
Momena Khatun, Mohammad Musharraf Uddin Bhuiyan, Jalal Uddin Ahmed, Aminul Haque, Mohammad Bozlur Rahman*, Mohammed Shamsuddin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 11 75-82 (8 pages)
the number of COCs recovered from each goat was counted and the oocytes retrieval efficiency was calculated. In experiment 2, the stages of oocyte nuclei upon aspiration (immature oocytes) with respect to prepubertal and pubertal goats were determined by nuclear staining. By observing nuclear stages, the nuclei were classified into germinal vesicle (GV), GV breakdown (GVBD), metaphase I (M... Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle's salt with L-glutamine and sodium bicarbonate) for 27 h at 39C under 5% CO2 in humidified air. The... -
Microtubule distribution in somatic cell nuclear transfer bovine embryos following control of nuclear remodeling type
Dae-Jin Kwon, Yu-Mi Lee, In-Sun Hwang, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 1 93-101 (9 pages)
o C, 5% CO2 in air for 16∼20 h. In one experiment, IVF embryos were treated with caffeine for 6 h or roscovitine for 1.5 h at the beginning of culture to evaluate the effects of chemicals on the microtubule distribution of embryos at the first mitotic phase. Immunofluorescent staining and confocal microscopy For the confocal microscopy, fused embryos were fixed 15 min, 3 h and 20 h after... This study was conducted to evaluate the microtubule distribution following control of nuclear remodeling by treatment of bovine somatic cell nuclear transfer (SCNT) embryos with caffeine or roscovitine. Bovine somatic cells were fused to enucleated oocytes treated with either 5 mM caffeine or 150 mM roscovitine to control the type of nuclear remodeling. The proportion of embryos that underwent premature chromosome condensation (PCC) was increased by caffeine treatment but was reduced by... -
Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos
Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 11 131-137 (7 pages)
the administration of IP3 and ryanodine by microinjection, may play a role in the mobilization of Ca 2+ stores, and affect the developmental competence. This hypothesis was effectively borne out by experiment 2. Development of parthenogenetically activated oocytes by electroporation of 25mM IP3 alone or 25mM IP3 and 10 mM ryanodine together followed by 6-DMAP treatment, was examined in experiment... of the electroporation experiment. For electroporation, cleavage rates were significantly higher in the IP3+ryanodine group than in the IP3 or control group (85.6% vs 73.7% or 67.6%, respectively). In the subsequent stages of embryonic development, such as morula and blastocyst formation, the IP3 and ryanodine group exhibited significantly higher rates of morula fomation than the IP3 or control groups (40.6% vs 24.2% or 16.7%, respectively). Similarly, the rate of blastocyst formation in the... -
Hydrogen peroxide attenuates refilling of intracellular calcium store in mouse pancreatic acinar cells
MiNaYoon, DongKwanKim, SeHoonKim, HyungSeoPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 11 233-239 (7 pages)
accumulation because this pump has abundant cysteine residues [37]. In this study, 300 mM of H2O2 failed to attenuate Ca 2+ extrusion through plasma membrane in TG- treated experiment. However 10 folds higher concentration of H 2O2 partially inhibited Ca 2+ extrusion to extracellular fluid under similar conditions (data not shown). These findings strongly suggest that the primary target for H... or Ca2+ extrusion through plasma membrane. Additionally, ruthenium red, a mitochondrial Ca2+ uniporter blocker, failed to attenuate H2O2-induced intracellular Ca2+ elevation. These results provide evidence that excessive generation of H2O2 in pathological conditions could accumulate intracellular Ca2+ by attenuating refilling of internal Ca2+ stores rather than by inhibiting Ca2+ extrusion to extracellular fluid or enhancing Ca2+ mobilization from extracellular medium in mouse pancreatic acinar...