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Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation
Sara Perteghella, Alessandro Gaviraghi, Silvia Cenadelli, Valeria Bornaghi, Andrea Galli, Barbara Crivelli, Barbara Vigani, Dani 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 11 81-88 (8 pages)
10 min, beads were collected by filtration through a nylon mesh cell strainer (Falcon 100 Cell Strainer; Corning, USA) with a 100 m pore size, rinsed twice with BULLXcell-egg yolk extender solution, and ..PAGE:3 Encapsulation and cryopreservation of buffalo and bovine semen 83 www.vetsci.org resuspended in the same solution. The 0.5 mL straws were then filled with the bead suspension by using... Friesian (Bos taurus) semen. Results obtained from in vitro analyses indicate that the encapsulation process does not have detrimental effects (compared to controls) on quality parameters (membrane integrity, progressive motility, path average velocity) in either species. Similarly, there were no detrimental effects after cryopreservation in either species. The fertilizing potential of encapsulated and cryopreserved semen was evaluated after AI in 25 buffalo and 113 bovine females. Pregnancy... -
Advanced Glycation End Products and Diabetic Complications
VarunParkashSingh, AnjanaBali, NirmalSingh, AmteshwarSinghJaggi 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 14 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.1 1 1-14 (14 pages)
decline in glomerular filtration rate, accompanied by proteinuria and other end-organ complications such as retinopathy [119]. Diabetic nephropathy progresses to end-stage renal disease via a number of stages including normal albu- minuria, incipient diabetic nephropathy, micro albuminuria and finally end-stage renal disease [120,121]. Progression to end stage renal disease is enhanced by... During long standing hyperglycaemic state in diabetes mellitus, glucose forms covalent adducts with the plasma proteins through a non-enzymatic process known as glycation. Protein glycation and formation of advanced glycation end products (AGEs) play an important role in the pathogenesis of diabetic complications like retinopathy, nephropathy, neuropathy, cardiomyopathy along with some other diseases such as rheumatoid arthritis, osteoporosis and aging. Glycation of proteins interferes with... -
Thyroid Hormone-Induced Alterations of Ryanodine and Dihydropyridine Receptor Protein Expression in Rat Heart
HaeWonKim, MiyoungPark, EunHeeLee, HyoungJinCho, HeeRanLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.3 11 329-337 (9 pages)
filtration (GF/C filters， Whatan， Clifton， New Jersey). Fi1ters were washed twice with 5 ml cold Tris-HCI buffer. The radioactivity of the fi1ters was counted in a scintillation counter at an effiiency of 39 41 %. The nonspecific CH]nitrendipine binding (in the presence of nitrendipine) was subtracted fom the total bindng (in the absence of nitrendipine) to obtain the specific binding of... In hyperthyroidism left ventricular contractility and relaxation velocity were increased, whereas these parameters were decreased in hypothyroidism. The mechanisms for these changes have been suggested to include alterations in the expression and/or activity levels of various proteins. In the present study, quantitative changes of ryanodine receptors and the dihydropyridine receptors, and the functional consequences of these changes in various thyroid states were investigated. In hyperthyroid... -
Thyroid Hormone-Induced Alterations of Ca2+-ATPase and Phospholamban Protein Expression in Cardiac Sarcoplasmic Reticulum
HaeWonKim, KyungMinNoh, MiyoungPark, HeeRanLee, EunHeeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.2 13 223-230 (8 pages)
modification of the Millipore filtration technique (Martonosi & Feretos， 1964). ln the Mil lipore filtration assay， the rate of Ca'+ uptake was determined in a medium containing 50 μg 01 SR protein per ml， 40 mM histidine-HC1， pH 7.0， 0.1 M KC1， 5 mM MgCh， 2.5 mM oxalate， 5 mM NaN 3， 0.5 mM EGTA， various amounts of "CaCh to yield 10 -7 10 -， M free Ca2-， using Millipore filters... hyperthyroidism left ventricular contractility and relaxation velocity were increased, whereas these parameters were decreased in hypothyroidism. The mechanisms for these changes have been suggested to include alterations in the expression and/or activity levels of various proteins; α-myosin heavy chain, β- myosin heavy chain, β-receptors, the guanine nucleotide-binding regulatory protein, and the sarcolemmal Ca2-ATPase. All these cellular alterations may be associated with changes in... -
Effect of Cisplatin on Na+/H+ Antiport in the OK Renal Epithelial Cell Line
JeeYeunKim, YangSaengPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.1 9 69-76 (8 pages)
Sev eral authors have described the efects of this drug on the renal structure and functions (Dentino et al， 1978; Dobyan et al， 1980; Daugaard et al， 1987). Reduction in glomerular filtration rate (Winston & Safirstein， 1985; Daugaard et al， 1988)， impaired urine concentrating abi1ity (Safirstein et al， 1982; 1986)， renal potassium and magnesium wasting Corresponding to: Yang... -
알부민 Gylcation이 약물의 단백질결합에 미치는 영향
배진우(Jin Woo Bae) 대한약리학회 대한약리학잡지 6 Pages
대한약리학회 대한약리학잡지 1995, 제 31권 제 1호 14 135-140 (6 pages)
g glucose were dissolved in PBS to become the final volume of 200 ml. After filtration of this solution by 0.2 m sterile syringe filter (Nalgene, USA), 100 ml solu- tion was incubated for 15 days at 37°C water bath with slight shaking (Julabo, Germany) and then dialysed by dialysis tube (MWCO 12,000~ 14,000, Spectra pore) against 2000 ml PBS for 3 times... Glycation이란 단당류의 카보릴기와 아미노산의 입실론 아미노기가 공유결합에 의하여 형성되는 반응으로 이는 단백질의 생리적 기능을 변화시키며 아울러 당뇨합병증을 유발한다. 본 연구에서는 warfarin과 dansylsarcosine의 단백질결합에 미치는 glycation의 영향을 평형투석법을 이용하여 연구하였으며 평형투석은 섭씨 37도의 진탕수조에서 3시간 동안 실시하였다. 약물의 농도가 알부민의 농도보다 높을 경우, 50 ± 16%가 glycation된 알부민은 8.5 ± 5.28% glycation 알부민을 함유한 정상알부민에 비해 약물과의 결합도가... -
부자(附子) Butanol fraction의 강심작용(强心作用)에 관한 연구(硏究)
홍사악(Hong S.A.), 박찬웅(Park C.W.), 김명석(Kim M.S.), 신상구(Shin S.G.), 대한약리학회 대한약리학잡지 8 Pages
대한약리학회 대한약리학잡지 1975, 제 11권 제 1호 1 7-14 (8 pages)
"ii.} ½ ~-"'J:%}~4- llfj-=f butanol ¾~ ½(AF(5) )~ &s.:~·(:i'\t~Ji){, Aconitum volubile Pallas var. pubescens Regel)¾ Fig. loJl A7 9t 7-Jo] 7-j ij %}.:,j ~½%}~ t:-\-. Aconiti Tuber Reflux Filtration 95% EtoH Evaporation to Dryness ! EtOH residues (1) Alkalinization Partitioning Neutralization Filtration 1% Na2COa Ether, CHCl3 Basic compounds (2) (Alkaloid) 1% HCI Evaporati... -
Effects of Insulin and IGFs on Phosphate Uptake in Primary Cultured Rabbit Renal Proximal Tubule Cells
Han, Ho-Jae, Park, Kwon-Moo 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1996, 제 30권 제 1호 7 63-76 (14 pages)
human subjects, recombinant human IGF-I administration stimulated renal plasma flow and the glomerular filtration rate, increased microproteinuria and tubular phosphate reabsorption, but caused no change in calcium reabsorption. The cellular systems that mediates this hormonal response remains to be investigated. The factors which are involved in the control of epithelial transport systems... The aim of present study was to characterize phosphate uptake and to investigate the mechanism for the insulin and insulin-like growth factor(IGF) stimulation of phosphate uptake in primary cultured rabbit renal proximal tubule cells. Results were as follows : 1. The primary cultured proximal tubule cells had accumulated 6.68±0.70 nmole phosphate/mg protein in the presence of 140 mM NaCl and 2.07±0.17 nmole phosphate/mg protein in the presence of 140 mM KCl during a 60 minute uptake period.... -
A Study on Na+ and Water Reabsorption in the Nephron Segment Beyond Proximal Tubule Measured by Lithium Clearance
Han, O-Soo, Goo, Yong-Sook, Sung, Ho-Kyung 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 9 189-200 (12 pages)
re- mained ADH action since after 30 min FrH2O was increased. However FrNa fluctuated a little at con- stant level in spite of great decrease of CL,, Na+ reabsorption at T ALH is about 15-20 % of filtration and it is increased by sever- al hormones (Hebert & Andreoli, 1984). Na+ reabsorption at TALH and distal tubule is influenced greatly by hormones; ADH, glu- cagon, PTH, PG, CT, aldosteron... During the past few years it has been proposed that lithium clearance can be used as a reliable measure for the outflow of tubular fluid from the proximal tubule. This study was aimed to characterize the inflow dependent reabsorption of Na in renal tubule beyond the proximal tubule. For this purpose, lithium clearance was used as a measure for the inflow from the proximal tubule and the changes in reabsorption fraction of Na and water were determined in rabbits. Rabbits were pretreated with... -
Inhibition of Dicarboxylate Transport by p-chloromercuribenzoic Acid (PCMB) in Plasma Membrane Vesicles of Rabbit Proximal Tubule
Kim, Yong-Keun, Kim, Tae-In, Jung, Jin-Sup, Lee, Sang-Ho 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 8 179-188 (10 pages)
a rapid filtration technique. Briefly, the reaction was initiated by adding mem- brane vesicles to the incubation medium (a I : 10 dilution of membrane vesicle suspen- sion) containing [' 4C] succinate at 25°C. The composition of the incubation medium was given in the figure legends. At the designated times, 100 I aliquots were taken and quickly filtered under vacuum through Millipore fil-... Effect of a sulfhydryl reagent, p-chloromercuribenzoic acid (PCMB), on the transport of succinate was studied in brush border (BBMV) and basolateral (BLMV) membrane vesicles isolated from rabbit renal cortex. PCMB induced an irreversible inhibition of the Na+-dependent succinate uptake in a dose-dependent manner with IC50 of 55 and 65 μM in BBMV and BLMV, respectively. The inhibitory effect of PCMB was prevented by a pretreatment of vesicles with dithiothreitol. PCMB did not increase Na+...