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Application of oral fluid samples to analyze the status quo of porcine reproductive and respiratory syndrome virus at herd level
Je min Han, Chae hyun Kim, Nak hyung Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 186 433-434 (2 pages)
Disease division, Animal and Plant Quarantine Agency Introduction: Clostridiumtetani is anaerobic, spore-forming bacteria, and theetiologic agent of the muscle-spasming disease tetanus. Deep penetrating woundsprovide the anaerobic conditions required for the bacteria to grow and releasetetanus toxin, tetanospasmin, and therefore are the most common source ofinfectio n. We report here a case... -
Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer
Anukul Taweechaipaisankul, Geon A Kim, Jun-Xue Jin, Su Cheong Yeom, Byeong Chun Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 3 492-499 (8 pages)
blood group A were found in isolated cell lines from piglets No. 2, No. 3, No. 6, and No. 7, and forming the positive control. Panels B and C in Fig. 1 shows expression of H-antigen-related genes (FUT1 and FUT2) in isolated cell lines of the piglets. The ..PAGE:5 496 Anukul Taweechaipaisankul et al. Journal of Veterinary Science Fig. 3. Relative glycol-protein quantification obtained by... Due to their similarities with humans in anatomy, physiology, and genetics miniature pigs are becoming an attractive model for biomedical research. We aim to establish and evaluate blood type O cells derived from Korean native pig (KNP), a typical miniature pig breed in Korea. Ten cell lines derived from 8 KNP piglets and one adult female KNP (kidney and ear tissues) were established. To confirm the presence of blood type O, genomic DNA, fucosyltransferase (FUT) expression, and... -
Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues
Bonn Lee, Hee Young Kang, Dong Oh Lee, Changhwan Ahn, Eui-Bae Jeung 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 2 445-451 (7 pages)
cation-barrier, facilitates NaCl reabsorption [15], and has tightening effect when forming TJs [27]. In the lung, claudin-4 protein was positively expressed by correlating with alveolar fluid clearance [25]. In the testis, all four claudins were observed in Leydig cells and/or the interstitial region, although the distribution patterns were slightly different, with claudin-2 detected in the... Claudins, which are known as transmembrane proteins play an essential role in tight junctions (TJs) to form physical barriers and regulate paracellular transportation. To understand equine diseases, it is helpful to measure the tissue-specific expression of TJs in horses. Major equine diseases such as colic and West Nile cause damage to TJs. In this study, the expression level and distribution of claudin-1, -2, -4, and -5 in eight tissues were assessed by Western blotting and... -
Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)
6 cells. The results showed that the hTERT-bMGEs group had higher colony forming efficiency than bMGEs (Table 1). The hLYZ expressed in hTERT-bMGEs To further confirm recombinant protein expression in hTERT-bMGE, the hLYZ expression of hLYZ-positive hTERT-bMGEs was identified by RT-PCR and western blot analysis. The results showed that the hLYZ gene (850 bp) and the hLYZ protein (14.7 kDa)... Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated... -
Time-dependent low-field MRI characteristics of canine blood: an in vitro study
Jimo Jeong, Sangjun Park, Eunseok Jeong, Namsoo Kim, Minsu Kim, Yechan Jung, Youngkwon Cho, Kichang Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 13 103-109 (7 pages)
to the ferric state (Fe 3+), forming methemoglobin [5]. Interestingly, a partial new layer in the MR signal developed in the blood clots by day 3 to 4. The signal changes that were observed include hyper intensity in T1W, and hypo intensity in TSE. The T2W findings were consistent with the guidelines of parenchymal hemorrhage in MR [1,5,6,10,23]. However, there were no significant changes in... blood samples were collected from eight healthy beagle dogs. Samples were placed in 5-mL tubes and imaged within 3 hours of collection at 1 day intervals from day 1 to day 30. The following sequences were used: T1-weighted (T1W), T2-weighted (T2W), fluid-attenuated inversion recovery (FLAIR), short tau inversion recovery (STIR), and T2-star gradient-echo (T2*-GRE). Visual comparison of the images revealed that four relatively homogenous blood clots and twelve heterogeneous blood clots developed.... -
Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
Marie M Meyerholz, Kirsten Mense, Michael Lietzau, Ana Kassens, Matthias Linden, Hendrike Knaack, Elisa Wirthgen, Andreas Hoefli 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 4 413-421 (9 pages)
of the present study, because two important parameters were not addressed; namely, free IGF1 and the acid labile subunit, forming a tertiary complex with IGFBP3 and IGF1. In general, changes within the maternal IGF1 and IGFBP concentrations during the cycle could play a role in preparing the maternal metabolism for a potential pregnancy. Notably, IGFBP4 concentrations were lower in pregnant... This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The concentrations... -
Computed tomographic evaluation of cervical vertebral canal and spinal cord morphometry in normal dogs
Eunjeong Seo, Jihye Choi, †, Mincheol Choi, Junghee Yoon* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 3 187-193 (7 pages)
vertebral and spinal cord structures according to body size have been reported in the literature [6,12,17]. In small dogs, the subarachnoid space is relatively narrow and conforms closely to bones forming the vertebral canal in the thoracolumbar region. In contrast, large breeds tend to have relatively thinner spinal cords and a uniform, parallel-sided subarachnoid space [12]. In the presen... The height, width, and cross-sectional area of the vertebral canal and spinal cord along with the area ratio of spinal cord to vertebral canal in the cervical vertebra were evaluated in images obtained using computed tomography (CT). Measurements were taken at the cranial, middle, and caudal point of each cervical vertebra in eight clinically normal small breed dogs (two shih tzu, two miniature schnauzers, and four mixed breed), 10 beagles, and four German shepherds. CT myelography facilitated... -
An overview of calf diarrhea - infectious etiology, diagnosis, and intervention
Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)
a Gram-positive, spore- forming anaerobic bacterium that causes a wide range of diseases in mammals and birds [137]. These microorganisms can be subdivided into five toxin types (A, B, C, D, and E) based on the production of four major toxins: alpha (α), beta (β), epsilon (ε), and iota (ι) [111]. Type A strains produce α toxin alone, type B stains produce α, β, and ε toxins; type C... Calf diarrhea is a commonly reported disease in young animals, and still a major cause of productivity and economic loss to cattle producers worldwide. In the report of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or... -
Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors
Andrea Maria Blanc, Mabel Beatriz Berois, Lorena Magalí Tomé, Alberto L. Epstein, Juan Ramón Arbiza* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 9 59-65 (7 pages)
Vero-7b cells infected at multiplicity of infection (MOI) of 0.1 plaque forming unit (PFU)/cell in Medium 199 (Invitrogen, USA) supplemented with 1% FBS (M199 1% FBS). When a complete cytophatic effect (CPE; round cells forming grape-like clusters) was observed (48∼72 h post-infection), the virus was harvested and concentrated using the following technique. A first round of centrifugation... Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected... -
Evaluation of infectivity and transmission of different Asian foot-and-mouth disease viruses in swine
Juan M. Pacheco, P. W. Mason 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 7 133-142 (10 pages)
(Cathay and PanAsia). When studied in cattle, FMDV infectivity can be readily accomplished by inoculating graded dilutions of virus intradermally into sites in the tongue, with scoring of lesions forming 24 to 72 h later [16,17]. However, the epidermis of the porcine tongue is thinner and more fragile than the bovine tongue, preventing application of this method to pigs [6]. Here we describe... Most isolates of foot-and-mouth disease virus (FMDV) display a broad host range. Since the late 1990s, the genetic lineage of PanAsia topotype FMDV serotype O has caused epidemics in the Far East, Africa, the United Kingdom, France, the Netherlands, and numerous other countries throughout Europe and Asia. In contrast, there are several FMDV isolates that exhibit a more restricted host range. A Cathay topotype isolate of FMDV serotype O from the 1997 epizootic in Taiwan (O/TAW/97) demonstrated...