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Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China
Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, L.Willems, Hongfei Zhu, Shaohua Hou 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 3 339-349 (11 pages)
structural proteins of PRRSV. Residues 13 and 151 in the GP5 proteins of PRRSVs have been shown to be associated with virulence [1]. Compared to the first Chinese strain, CH-1a, the other PRRSV strains exhibit variation in their potential glycosylation level or sites. Based on our results, residues 13 and 151 in the GP5 proteins of PRRSVs are associated with virulence. With the changing and... syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple genomic variations in Nsp2, a unique N-glycosylation... -
Time-dependent proteomic and genomic alterations in Toll-like receptor-4-activated human chondrocytes: increased expression of lamin A/C and annexins
SeungHeeHa, HyoungKyuKim, NguyenThiTuyetAnh, NariKim, KyungSooKo, ByoungDooRhee, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 16 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.5 8 531-546 (16 pages)
A LMNA Structural constituent of cytoskeleton Q9BR76 Coronin-1B CORO1B Intracellular protein transport Structural constituent of cytoskeleton P30040 Endoplasmic reticulum protein ERp29 ERP29 Exocytosis P40261 Nicotinamide N-methyltransferase NNMT Metabolic process Methyltransferase activity Q9Y230 RuvB-like 2 RUVBL2 Nucleobase, nucleoside, nucleotide and nucleic acid metabolic process DNA... we determined the proteomic and genomic alterations in human chondrocytes during lipopolysaccharide (LPS)-induced inflammation to elucidate the underlying mechanisms and consequences of TLR-4 activation. Human chondrocytes were cultured with LPS for 12, 24,and 36 h to induce TLR-4 activation. The TLR-4-induced inflammatory response was confirmed by real-time PCR analysis of increased interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) expression levels. In... -
Diseases Severity, Genetic Variation, and Pathogenicity of Ceratocystis Wilt on Lansium domesticum in South Sumatra, Indonesia
Ahmad Muslim, Rahmat Pratama, Suwandi Suwandi, Harman Hamidson 한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 6 131-145 (15 pages)
the structural dimensions of all isolates for ascomata, ascospores, and chlamydospores (Table 3). All reported isolates were in the range of C. fimbriata and showed relatively similar growth responses. They did not grow at 10°C and optimal growth for all Ceratocystis iso- lates occurred between 25°C and 30°C (Table 3). DNA extraction, amplification, sequencing, and phylo- genetic analyses.... Ceratocystis wilt disease has caused significant mortality in duku (Lansium domesticum) since 2014 and has now spread to all districts in South Sumatra, Indonesia. Recently, 16 isolates from duku representing populations from various districts in South Sumatra were isolated. Analysis for the morphological characteristic of the isolate showed that the population has a uniform morphology. Genetic analysis based on internal transcribed spacer (ITS) and β-tubulin sequences verified that the... -
Comparative Genomic Analysis of Pathogenic Factors of Pectobacterium Species Isolated in South Korea Using Whole-Genome Sequencing
Samnyu Jee, In-Jeong Kang, Gyeryeong Bak, Sera Kang, Jeongtae Lee, Sunggi Heu, Ingyu Hwang 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 1호 2 12-24 (13 pages)
Acids Res. 43:W39-W49. Bernal, P., Llamas, M. A. and Filloux, A. 2018. Type VI secre- tion systems in plant-associated bacteria. Environ. Microbiol. 20:1-15. Cascales, E. and Cambillau, C. 2012. Structural biology of type VI secretion systems. Philos. Trans. R. Soc. Lond. B Biol. Sci. 367:1102-1111. Coulthurst, S. 2019. The Type VI secretion system: a versatile bacterial weapon. Microbiology... type III secretion system (T3SS) and effectors, were identified in PZ1; T3SSa was not identified in HC. Two putative effectors, including hrpK, were identified in seven strains along with dspEF. We also identified 13 structural genes, six regulator genes, and five accessory genes in the type VI secretion system (T6SS) gene cluster of six Pectobacteri- um species, along with the loss of T6SS in PZ1. HC had two subsets, and JK2.1 had three subsets of T6SS. With the GxSxG motif, the phospholipase A... -
A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
Mahsa Abadkhah, Davoud Koolivand, Omid Eini 한국식물병리학회 The Plant Pathology Journal 18 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 7 514-531 (18 pages)
more sensitive method and sequences different genomic region of TSWV to confirm TSWV infection and also investigate the genetic variation of TSWV isolates from tomato plants. As noted before, virus symptoms were observed in most tomato fields that were visited in different regions; how- ever, necrotic spots on the leaves, common mosaic and yellowing were most common symptoms that observed in... primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively. The amplified fragments were cloned into pTG19-T and sequenced. Sequence comparisons with those available in the GenBank showed that the sequences belong to TSWV. The high nucleotide identity and similarities of new sequences based on the L, N, and NSs genes showed that minor evolutionary differences exist amongst the isolates. The phylogenetic tree grouped all isolates six clades based on... -
Production and characterization of genogroup II human Norovirus VP1-specific monoclonal antibodies
Yun Ju Kong, Jin Won Kim, Myeong Seob Kim, Hyeok Jin Lee, Sang Won Lee, Shien Young Kang 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 96 380-381 (2 pages)
infections and GII is the most prevalent genogroup. The NoV genome consists of a ~7.5 kb single-stranded, positive-sense RNA containing three open reading frames (ORFs). ORF1 encodes six non-structural proteins (NSP) for viral replication. The ORF2 encodes major capsid protein VP1 while ORF3 encodes the minor structural protein VP2. In this study, human NoV VP1 gene was expressed using a... -
Immune responses in pigs by vaccination of transgenic plant-expressed classical swine fever virus E2 protein
Ji hye Shin, Seong In Lim, Se Eun Choe, Ki sun Kim, Eun Ju Sohn, In Soo Cho, Dong Jun An 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 201 301-302 (2 pages)
polyprotein that is subsequently processed into for structural protein (nucleocapsid protein C, envelop glycoprotein E rns, E1, and E2) and eight nonstructural proteins. The glycoprotein E2 is the most immunogenic envelope protein. In previous studies, we have produced the CSFV glycoprotein E2 developed using recombinant protein expression system in transgenic Arabidopsis ( A. thaliana) and... -
Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages
Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang4, Chang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 5 358-367 (10 pages)
of PAM-passaged PRRSV strain 363 www.vetsci.org Fig. 5. Schematic representation of amino acid (aa) differences between the parental CA-2-P20 virus and its cell-adapted derivatives. The genomic sequences of the virus strains were determined as described in Table 1. The organization of the CA-2 genome (approximately 15 kb) is shown at the top. The coding regions for each nonstructural protein... and enhanced antibody responses in pigs. All pigs inoculated with CA-2-MP120 developed viremia and seroconverted to PRRSV. During 20 passages in PAM cells, CA-2-MP120 acquired 15 amino acid changes that were mostly distributed in nsp2 and minor structural protein-coding regions. Among these changes, 6 mutations represented reversions to the sequences of the reference CA-2 and parental CA-2-P20 strains. These genetic drifts may be hypothetical molecular markers associated with PRRSV macrophage... -
Control of type O foot-and-mouth disease by vaccination in Korea, 2014-2015
Jong-Hyeon Park, Dongseob Tark, Kwang-Nyeong Lee, Ji-Eun Chun, Hyang-Sim Lee, Young-Joon Ko, Soo-Jeong Kye, Yong-Joo Kim, Jae-Ku 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 13 271-279 (9 pages)
animals affected were pigs, but five cattle farms were also affected. This study examined the regional distribution of the December 2014 virus as well as the prevalence of antibodies to the viral structural proteins (SP) and nonstructural proteins (NSP) of the FMDV after the outbreak and additional vaccinations. The characteristics and genetic similarities between the FMDVs in Korea and tho... On December 3, 2014, a type O foot-and-mouth disease (FMD) outbreak began in Korea. Although vaccinations were administered, FMD cases increased steadily for five months, and reached 185 cases by April 2015. Most of the affected animals were pigs, which are vulnerable to vaccination. The FMD virus belonged to the South-East Asia (SEA) topotype that had been observed three times in Korea between April 2010 and July 2014. However, the FMD virus isolated in December 2014 had a unique feature; that... -
Polymorphism in the serotonin transporter protein gene in Maltese dogs with degenerative mitral valve disease
Chang-Min Lee, Jae-Ik Han, Min-Hee Kang, Seung-Gon Kim, Hee-Myung Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 16 129-135 (7 pages)
16 Protein structural modeling and protein structure prediction of polymorphisms were evaluated via the web server RaptorX [18]. Alpha helix, beta sheet, and loop structures were predicted for each secondary structure. uGDT, unnormalized global distance test; Ile, isoleucine; Phe, phenylalanine; Lys, Lysine; Arg, arginine; Glu, glutamine; Val, valine; Gly, glycine. Polymorphism prediction and... gene of Maltese dogs and DMVD. Genomic DNA was extracted from blood samples collected from 20 client-owned DMVD Maltese dogs and 10 healthy control dogs, and each exon of the SERT gene was amplified via polymerase chain reaction. The resulting genetic sequences were aligned and analyzed for variations by comparing with reference sequences; the predicted secondary structures of these variations were modeled and cross-verified by applying computational methods. Genetic variations, including five...