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Reverse Transcription-loop-mediated Isothermal Amplification (RT-LAMP) Assay for the Rapid Detection of Swine Influenza Viruses
Eun Mi Kim, Hyo Sung Jeon, Hee Jung Kim, Sang Geon Yeo, Choi Kyu Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 178 231-232 (2 pages)
is an acute respiratory disease, and its severity depends on many factors, including host age, virus strain, and secondary infections. Pigs have been postulated to play an im portant role in interspecies transmission to humans by acting as the ‘‘mixing vessel’’ for reassortment between viruses specific to different host species. To control the pandemic influenza A H1N1 infection, early... -
Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene
Sharad Saurabh, Shubhankar Sircar, Jobin J. Kattoor, Souvik Ghosh, Nobumichi Kobayashi, Krisztian Banyai, Obli R. VinodhKumar, U 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 6 35-43 (9 pages)
of sequence variation [16]. The interspecies variable domain (ISVD; spanning the 135th to 146th amino acid residues), a flexible region located at the extreme C-terminus, is critical for RV virulence and its diarrhea-inducing capabilities; mutations at the 135th and 139th amino acid residues result in loss of viroporin function [44]. Extensive primary structure analyses of NSP4 sequences,... Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes... -
Analgesic efficacy of oral firocoxib in ovariohysterectomized cats
Prangtip Phuwapattanachart, Naris Thengchaisri 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 7 175-182 (8 pages)
Vet Rec 2006, 159, 309-313. 10. Court MH, Greenblatt DJ. Molecular basis for deficient acetaminophen glucuronidation in cats. An interspecies comparison of enzyme kinetics in liver microsomes. ..PAGE:8 182 Prangtip Phuwapattanachart, Naris Thengchaisri Journal of Veterinary Science Biochem Pharmacol 1997, 53, 1041-1047. 11. Curry SL, Cogar SM, Cook JL. Nonsteroidal antiinflammatory drugs: a... = 0.04); nonetheless, there was no significant difference in mean CPS between firocoxib-1 and control groups at all intervals. BMBT and body temperature were within normal limits in all groups. However, reversible azotemia was identified in two firocoxib-3 cats at 72 h post-operation. One firocoxib-3 cat vomited once at 48 h post-operation. In conclusion, firocoxib-3 is helpful for postoperative pain control in cats; however, gastrointestinal irritation and renal function side effects may occur. -
Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease
Magdalena Goły?ska, Izabela Polkowska, Małgorzata Bartoszcze-Tomaszewska, Aleksandra Sobczy?ska-Rak, Łukasz Matuszewski 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 7 51-58 (8 pages)
a collective sample. The results of the study revealed interspecies similarities in the prevalences of Porphyromonas (P gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum. Red complex bacteria comprised the largest portion of the studied bacterial complexes in all study groups, with P being the most commonly isolated bacterium. The results show similarities in the... of the study revealed interspecies similarities in the prevalences of Porphyromonas (P.) gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum. Red complex bacteria comprised the largest portion of the studied bacterial complexes in all study groups, with P. gingivalis being the most commonly isolated bacterium. The results show similarities in the prevalence of bacterial microflora in dogs and humans. Microbiological analysis of gingival pockets by using rapid... -
Post-pandemic seroprevalence of human influenza viruses in domestic cats
Mahmoud Ibrahim, Ahmed Ali, Joshua B. Daniels, Chang-Won Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 10 515-521 (7 pages)
A viruses are generally host-specific, interspecies transmission has been reported among different species worldwide [39]. The susceptibility of cats to human H2N2 and H3N2 [11,24], seal H7N7 and avian H7N3 viruses [11] was initially reported in the 1970s. Epidemiological studies of influenza in cats have been limited, and recent studies of influenza in cats have primarily focused on the... The continuous exposure of cats to diverse influenza viruses raises the concern of a potential role of cats in the epidemiology of these viruses. Our previous seroprevalence study of domestic cat sera collected during the 2009 H1N1 pandemic wave (September 2009?September 2010) revealed a high prevalence of pandemic H1N1, as well as seasonal H1N1 and H3N2 human flu virus infection (22.5%, 33.0%, and 43.5%, respectively). In this study, we extended the serosurvey of influenza viruses in cat sera... -
Hematologic and serum biochemical reference intervals of the Oriental white stork (Ciconia boyciana) and the application of an automatic hematologic analyzer
Jae-Ik Han, Hye-Jin Jang, Ki-Jeong Na 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 18 399-405 (7 pages)
with those for the black stork C, which showed greater levels of Hb, total protein, ALP, and triglyceride in females, while males showed higher levels of albumin [19], probably reflecting interspecies variability. In conclusion, this study determined hematological and serum biochemistry profiles for evaluating the health status of the Oriental white stork C. We also demonstrated that the... This study was conducted to establish accurate baseline values of clinical laboratory data with regard to age-related changes in the Oriental white stork (Ciconia boyciana). In addition, the availability of an automated hematological cell counter was evaluated. A total of 94 clinically normal storks, including 64 young storks (<1 year old; 30 male and 34 female) and 30 adults (> 1 year old; 17 male and 13 female) were included. Hematological assays were performed using manual and automated... -
Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)
blastocysts produced by IVF, ICSI and SCNT. Zygote 2014, 22, 505-512. 20. Ma LB, Cai L, Li JJ, Chen XL, Ji FY. Two-staged nuclear transfer can enhance the developmental ability of goat-sheep interspecies nuclear transfer embryos in vitro. In Vitro Cell Dev Biol Anim 2011, 47, 95-103. 21. Martins LT, Gaudencio Neto S, Aguiar LH, Calderon CEM, Tavares KCS, Carneiro IS, Morais AS, Girao Neto... Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated... -
A novel M2e-multiple antigenic peptide providing heterologous protection in mice
Feng Wen, Ji-Hong Ma, Hai Yu, Fu-Ru Yang, Meng Huang, Yan-Jun Zhou, Ze-Jun Li, Xiu-Hui Wang, Guo-Xin Li, Yi-Feng Jiang, Wu Tong, 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 9 71-78 (8 pages)
species, with avian and swine strains presenting the greatest potential for interspecies transmission. Since the 1990s, H3N2 triple-reassortant viruses with genes derived from human (HA, NA, and PB1), swine (N, M, and NS), and avian (PB2 and PA) influenza viruses have caused outbreaks of respiratory disease in pigs throughout North America [14,25]. Yu et al. [22] summarized and reported the... Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by... -
Assessment of the safety and efficacy of low pathogenic avian influenza (H9N2) virus in inactivated oil emulsion vaccine in laying hens
Jeong-Hwa Shin, Jong Seo Mo, Jong-Nyeo Kim, In-pil Mo, Bong-Do Ha 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 4 27-34 (8 pages)
26-33. 17. Lee HJ, Kwon JS, Lee DH, Lee YN, Youn HN, Lee YJ, Kim MC, Jeong OM, Kang HM, Kwon JH, Lee JB, Park SY, Choi IS, Song CS. Continuing evolution and interspecies transmission of influenza viruses in live bird markets in Korea. Avian Dis 2010, 54 (Suppl 1), 738-748. 18. Lu H, Castro AE, Pennick K, Liu J, Yang Q, Dunn P, Weinstock D, Henzler D. Survival of avian influenza virus H7N2 in... In Korea, several outbreaks of low pathogenic AI (H9N2) viral infections leading to decreased egg production and increased mortality have been reported on commercial farms since 1996, resulting in severe economic losses. To control the H9N2 LPAI endemic, the Korea Veterinary Authority has permitted the use of the inactivated H9N2 LPAI vaccine since 2007. In this study, we developed a killed vaccine using a low pathogenic H9N2 AI virus (A/chicken/Korea/ADL0401) and conducted safety and efficacy... -
An overview of calf diarrhea - infectious etiology, diagnosis, and intervention
Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)
account for maldigestive/ malabsorptive diarrhea promoted by rotavirus infection. Viral infection causes villus atrophy and usually affects the caudal part of the small intestine. Evidence for interspecies transmission along with genetic reassortment between human and animal rotaviruses (e.g., swine, bovine, feline, and canine) has raised concerns about zoonotic rotaviruses [81]. Bovine... Calf diarrhea is a commonly reported disease in young animals, and still a major cause of productivity and economic loss to cattle producers worldwide. In the report of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or...


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