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A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer
Qing Wang, Mianmian Chen, Wei Zhang 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 12 260-270 (11 pages)
using a previously described protocol [19]. Briefly, the rRNA was removed, and the mRNA was extracted and fragmented by adding a fragmentation buffer. First-strand cDNA was then generated by randomly primed reverse transcription and the second-strand cDNA was synthesized using dNTPs, RNase H, and DNA polymerase I. The QIAquick PCR extraction kit (Qiagen, Germany) was used to purify the cDNA... Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction... -
Effects of mosapride on motility of the small intestine and caecum in normal horses after jejunocaecostomy
Kouichi Okamura, Naoki Sasaki*, Takuya Kikuchi, Aya Murata, Inhyung Lee, Haruo Yamada, Hisashi Inokuma 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 10 157-160 (4 pages)
a protocol approved by the Obihiro University Institutional Animal Care and Use Committee. Surgical procedure All foods and water were withheld for 12 h before the surgery. All horses were intravenously pre-medicated with 4 μg/kg of medetomidine (Domitor; Orion, Japan), and anesthesia was intravenously induced 5 min later with 0.03 mg/kg of diazepam (10 mg, Horizon; Yamanouchi ..PAGE:2 158... The purpose of the present study was to evaluate the prokinetic effects of mosapride with non-invasive assessment of myoelectrical activity in the small intestine and caecum of healthy horses after jejunocaecostomy. Six horses underwent celiotomy and jejunocaecostomy, and were treated with mosapride (treated group) at 1.5 mg/kg per osos once daily for 5 days after surgery. The other six horses did not receive treatment and were used as controls (non-treated group). The electrointestinography... -
Anatomy Histology : Involvement of macrophages in germ cell death in the rat testis with acute experimental testicular torsion
ChangJongMoon, TaeKyunShin 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2004, 제 44권 제 3호 2 329-334 (6 pages)
protocol for all animal experiments was approved by the Committee for Animal Experimentation at the University of the Ryukyus. Adult male Sprague Dawley rats(250-300 g) were maintained on a 12:12-h light:dark cycle. Rats were anesthetized for surgery with sodium pentobarbital(50 mg/kg body weight, i.p.) and all procedures were conducted using aseptic techniques. The left scrotum was incised and... -
Nortriptyline, a tricyclic antidepressant, inhibits voltage-dependent K+ channels in coronary arterial smooth muscle cells
SungEunShin, HongliangLi, HanSolKim, HyeWonKim, MiSeonSeo, Kwon-SooHa, Eun-TaekHan, Seok-HoHong, AmyL.Firth, Il-WhanChoi6, YoungMinBae, Won 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 10 225-232 (8 pages)
y=1/{1+exp((VV1/2)/k)} where V is the test potential, V1/2 represents the half-point of activation, and k is the slope factor. Steady-state inactivation was calculated using a two-step voltage protocol from a test potential of +40 mV for 600 ms after 7-s of preconditioning pulses applied at potentials ranging from 80 to +30 mV in the absence and presence of nortriptyline. The steady-state... We demonstrated the effect of nortriptyline, a tricyclic antidepressant drug and serotonin reuptake inhibitor, on voltage-dependent K+ (Kv) channels in freshly isolated rabbit coronary arterial smooth muscle cells using a whole-cell patch clamp technique. Nortriptyline inhibited Kv currents in a concentration-dependent manner, with an apparent IC50 value of 2.86±0.52 µM and a Hill coefficient of 0.77±0.1. Although application of nortriptyline did not change the activation curve,... -
Seasonal acclimation in sudomotor function evaluated by QSART in healthy humans
YoungOhShin, Jeong-BeomLee, Jeong-HoKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.5 7 499-505 (7 pages)
winter. This protocol was approved by the University of Soonchunhyang research committee. Written informed consent was obtained from all participants after a thorough explanation of the study including its purpose and risks, the procedures complied with the 2000 Declaration of Helsinki of the World Medical Association. Fig. 1. monthly mean daily average ambient temperatures from July 2012 to... The quantitative sudomotor axon reflex testing (QSART) is a classic test of routine postganglionic sudomotor function. We investigated sudomotor function by QSART after summer (July 2012) and winter (January 2013) seasonal acclimation (SA) in the Republic of Korea. QSART with acetylcholine (ACh) iontophoresis were performed to determine directly activated (DIR) and axon reflex-mediated (AXR1, 2) sweating rate. Onset time of axon reflex, activated sweat gland density (ASGD), activated sweat gland... -
Differential Gene Expression in GPR40-Overexpressing Pancreatic β-cells Treated with Linoleic Acid
In-SuKim, So-YoungYang, Joo-HuiHan, Sang-HyukJung, Hyun-SooPark, Chang-SeonMyung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.2 9 141-149 (9 pages)
the manufacturer’s protocol (RBCBioscience, New Taipei, Taiwan). The quality of total RNA was confirmed by run- ning samples on an Agilent 2100 Bioanalyzer (Agilent Technology, CA, USA). RIN (RNA Integrity Number, cut-off threshold: 7.0) values were 9.6 for RIN-5f and RIN-40, and 9.7 for RIN-40 treated linoleic acid. Agilent’s Rat Oligo Microarray (44K) analysis was carried out at eBiogen... in RIN-5f versus RIN-40. Moreover, the differential genetic profiles were investigated in RIN-40 cells treated with 30 μM linoleic acid, which resulted in selection of 93 genes in RIN-40 versus RIN-40 treated with linoleic acid. Based on the Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG, http://www.genome.jp/kegg/), sets of genes induced differentially by treatment with linoleic acid in RIN-40 cells were found to be related to mitogen-activated protein (MAP) kinase- and neuroactive... -
A Computational Model of Cytosolic and Mitochondrial [Ca2＋] in Paced Rat Ventricular Myocytes
JaeBoumYoum, SeongWooChoi, ChangHanJang, HyoungKyuKim, ChaeHunLeem, NariKim, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 23 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.4 5 217-239 (23 pages)
K ＋ current, rapid component (I Kr) The formulation of IKr is the same as that in Kyoto Model by Matsuoka et al [11]. The maximum conductance was ad- justed to reproduce the same current density (0.36 pA ㆍpF 1 ) from rat ventricular myocytes with the same voltage-clamp protocol [25]. Inward rectifier K ＋ current (I K1) The inward rectifier K ＋ current (IK1) contributes most to the... We carried out a series of experiment demonstrating the role of mitochondria in the cytosolic and mitochondrial Ca2＋ transients and compared the results with those from computer simulation. In rat ventricular myocytes, increasing the rate of stimulation (1∼3 Hz) made both the diastolic and systolic [Ca2＋] bigger in mitochondria as well as in cytosol. As L-type Ca2＋ channel has key influence on the amplitude of Ca2＋-induced Ca2＋ release, the relation between stimulus frequency and the... -
Effect of the Heat-exposure on Peripheral Sudomotor Activity Including the Density of Active Sweat Glands and Single Sweat Gland Output
Jeong-BeomLee, Tae-WookKim, Young-OhShin, Young-KiMin, Hun-MoYang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.5 4 273-278 (6 pages)
the axon reflex response (AXR) at the point of stimulation using the QSART after ACh ionphoresis before and after acclimation over 10 days. METHODS Subjects Following approval of the experimental protocol from the University of Soonchunhyang Research Committee and ob- taining written informed consent normotensive partic- ipants volunteered for the stud y. Twelve male volunteers, 22.6 years of... Tropical inhabitants are able to tolerate heat through permanent residence in hot and often humid tropical climates. The goal of this study was to clarify the peripheral mechanisms involved in thermal sweating pre and post exposure (heat-acclimatization over 10 days) by studying the sweating responses to acetylcholine (ACh), a primary neurotransmitter of sudomotor activity, in healthy subjects (n=12). Ten percent ACh was administered on the inner forearm skin for iontophoresis. Quantitative... -
Altered Vascular Calcium Regulation in Hypertension
Won-jaeKim, Jong-unLee, YongHyunPark, SangChaeNam 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.5 8 529-535 (7 pages)
before starting the experirental protocol Caffeine bas been kno‘vn to cause contraction by directly releasing calciur fror the sarcoplasric reticulur， being independent of second ressenger잉 (Kanaide et al， 1987). Therefore， to deplete the vascular ca1cium stoe， the aortic preparatlOns were exposed to repeated challenges with caffeine (35 rrolfL， for 5 r each) in the Ca2+ -free redia... The present study was aimed at investigating whether the vascular calcium regulation is altered in hypertension. Two-kidney, one clip (2K1C) and deoxycorticosterone acetate (DOCA)-salt hypertension were made in rats, and their thoracic aortae were taken 4 weeks later. The isometric contractile response and calcium uptake of the endothelium-denuded aortic preparations were determined. Caffeine (0.1∼35 mmol/L) induced a greater contraction in 2K1C and DOCA-salt hypertension than in normotensive... -
Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 5호 6 488-498 (11 pages)
tions eluted from the Sephadex LH-20 column with metha- nol were evaporated to dryness and loaded onto a Sep- Pak C18 cartridge (10 g) (Waters, Milford, MA, USA) with methanol-water. Using this protocol, 220 mg of compound 1 and 24 mg of compound 2 were obtained from 4 l of cul- ture supernatant. Electrospray ionization mass and tandem mass spec- trometry. Purified antifungal substances were... The aim of this study was to identify volatile and agar-diffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation...