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Multiple Signaling Pathways Contribute to the Thrombin-induced Secretory Phenotype in Vascular Smooth Muscle Cells
JiYoungJeong, YounghaeSon, Bo-YoungKim, Seong-KugEo, Byung-YongRhim, KoanhoiKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.6 10 549-555 (7 pages)
et al leased during injury or infl ammation, subendothelial SMCs will be come into contact wi th thrombin after endothelial injury and thrombus formatio n [15]. Moreover, due to pro- tection from inactivation by ci rculating inhibitors, thrombin bound to the subendothelial extracellular matrix remains functionally active [16], and SMCs are likely to be exposed to thrombin in athe rosclerotic... We attempted to investigate molecular mechanisms underlying phenotypic change of vascular smooth muscle cells (VSMCs) by determining signaling molecules involved in chemokine production. Treatment of human aortic smooth muscle cells (HAoSMCs) with thrombin resulted not only in elevated transcription of the (C-C motif) ligand 11 (CCL11) gene but also in enhanced secretion of CCL11 protein. Co-treatment of HAoSMCs with GF109230X, an inhibitor of protein kinase C, or GW5074, an inhibitor of Raf-1... -
Scant Extracellular NAD Cleaving Activity of Human Neutrophils is Down-Regulated by fMLP via FPRL1
Md.AshrafulHasan, Md.TipuSultan, Won-GyunAhn, Yeon-JaKim, Ji-HyeJang, Chang-WonHong, Dong-KeunSong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.6 8 497-502 (6 pages)
major immune cells to be recruited to the site of inflammation [16]. During tis- sue damage, NAD may be leaked to the extracellular envi- ronment [17]. At the inflammatory site, it may also be re- leased from a mechanically stressed cell to the extracellular environment [17]. Thus, released or leaked NAD may im- pose autocrine or paracrine effects on the intracellular sig- naling of a variety of... Extracellular nicotinamide adenine dinucleotide (NAD) cleaving activity of a particular cell type determines the rate of the degradation of extracellular NAD with formation of metabolites in the vicinity of the plasma membrane, which has important physiological consequences. It is yet to be elucidated whether intact human neutrophils have any extracellular NAD cleaving activity. In this study, with a simple fluorometric assay utilizing 1,N6-ethenoadenine dinucleotide (etheno-NAD) as the... -
Inhibitory Effects of Ginsenoside-Rb2 on Nicotinic Stimulation-Evoked Catecholamine Secretion
Hyo-JeongLim, Hyun-YoungLee, Dong-YoonLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.5 12 431-439 (9 pages)
of Rb2 on ACh- evoked CA secretory responses were significantly recovered to the control level in the pr esence of L-NAME. Therefore, it was decided directly to de termine the level of NO re- leased from rat adrenal medulla after the treatment of Rb2. Moreover, it has been found that ginsenosides reduce blood pressure via increases in prod uction of endothelial nitric oxide [12] and that... The aim of the present study was to investigate whether ginsenoside-Rb2 (Rb2) can affect the secretion of catecholamines (CA) in the perfused model of the rat adrenal medulla. Rb2 (3∼30 μM), perfused into an adrenal vein for 90 min, inhibited ACh (5.32 mM)-evoked CA secretory response in a dose- and time-dependent fashion. Rb2 (10 μM) also time-dependently inhibited the CA secretion evoked by DMPP (100 μM, a selective neuronal nicotinic receptor agonist) and high K+ (56 mM, a direct membrane... -
Roles of Non-cholinergic Intrapancreatic Nerves, Serotonergic Nerves, on Pancreatic Exocrine Secretion in the Isolated Perfused Rat Pancreas
ZhengerJiang, Bich-NaShin, In-HyeKim, HyunJooLee, Jun-HwanYong, Min-JaeLee, Moo-HoWon, Yun-LyulLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.5 9 307-312 (6 pages)
in the gastro- intestinal tract, the roles of serotonin in the gut are still unclear. It is mainly reported that roles of serotonin re- leased from enteric nervous system or enterochromaffin cells in the intestinal mucosa [3]. Thus this study was fo- cused to fined whether intrapancreatic serotonergic nerves could affect pancreatic exocrine secretion in the ex vivo model. The pancreas is a... a single injection of urethane. The median line and the abdominal aorta was carefully dissected and cannulated with PE-50 tubing just above the celiac artery, and then tightly ligated just below the superior mesenteric artery. The pancreatic duct was also cannulated with Tygon microbore tubing. With the addition of serotonin, pancreatic volume flow and amylase output were significantly inhibited electrical field stimulation (EFS). On the other hand, pancreatic volume flow and amylase output were... -
P2 Receptor-mediated Inhibition of Vasopressin-stimulated Fluid Transport and cAMP Responses in AQP2-transfected MDCK Cells
YangHooKim, YoungJinChoi, HaeRahnBae, JaeSukWoo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.1 2 9-14 (6 pages)
significance. It is re- leased as a cotransmitter at adrenergic nerve endings, through a conductive pathways such as cystic fibrosis trans- membrane conductance regulator (CFTR), and via a non- specific pathways from inflammatory and damaged cells etc. (Dubyak and El-Moatassim, 1993; North and Barnard, 1997; Williams and Burnstock, 1997). The circulating con- centration of ATP (to 1 micromolar)... We cultured canine kidney (MDCK) cells stably expressing aquaporin-2 (AQP2) on collagen-coated permeable membrane filters and examined the effect of extracellular ATP on arginine vasopressin (AVP)-stimulated fluid transport and cAMP production. Exposure of cell monolayers to basolateral AVP resulted in stimulation of apical to basolateral net fluid transport driven by osmotic gradient which was formed by addition of 500 mM mannitol to basolateral bathing solution. Pre-exposure of the basolateral... -
Effects of γ-Aminobutyric Acid on Intrinsic Cholinergic Action in Exocrine Secretion of Isolated, Perfused Rat Pancreas
YongDeukPark, HyungSeoPark, ZhengYunCui, HyoungJinPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 8 169-174 (6 pages)
Lifson et al, 1985). Although it is unknown at the present time whether GABA is re leased from the islet β-cells into the circulation, there are good evidences suggesting the release of GABA (Gaskins et al, 1995; Smismans et al, 1997). Because high affinity binding sites of GABA have been observed in pancreatic exocrine cells (Reusens-Billen et al, 1984), it is assumed that GABA might play... γ-Aminobutyric acid (GABA) has been reported to enhance exocrine secretion evoked not only by secretagogues but also by intrinsic neuronal excitation in the pancreas. The pancreas contains cholinergic neurons abundantly that exert a stimulatory role in exocrine secretion. This study was undertaken to examine effects of GABA on an action of cholinergic neurons in exocrine secretion of the pancreas. Intrinsic neurons were excited by electrical field stimulation (EFS; 15 V, 2 msec, 8 Hz, 45 min)... -
Correlation between mEPSC Amplitude and Rise Time upon the Blockade of AMPA Receptor Desensitization at Hippocampal Synapses
SuHyunJung, SukwooChoi 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.2 3 81-86 (6 pages)
2) others expand rapidly (a rapidly expanding fusion pore). While transmitters are re- leased almost instantaneously through the rapidly expand- ing fusion pore, transmitter release can be slowed down due to the non-expanding fusion pore (Bruns & Jahn, 1995). The fusion pore operation could be modulated by kinases, phos- phatases, and calcium levels, thereby regulating the speed of transmitter... Conventional views of synaptic transmission generally overlook the possibility of "postfusional- control" the regulation of the speed or completeness of transmitter release upon vesicular fusion. However, such regulation often occurs in non-neuronal cells where the dynamics of fusion-pore opening is critical for the speed of transmitter release. In case of synapses, the slower the transmitter release, the smaller the size and rate-of-rise of postsynaptic responses would be expected if... -
Noradrenergic Modulation of Spontaneous Inhibitory Postsynaptic Currents in the Hypothalamic Paraventricular Nucleus
LongHwaLee, WoneeChong, KihoLee, JinBongPark, PanDongRyu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.2 2 71-80 (10 pages)
and/or neuro- hormones and neuropeptides. Another important factor that affects the neuronal excitability is fast and slow synap- tic currents mediated by various neurotransmitters re- leased from the presynaptic neurons. PVN neurons receive both glutamatergic and GABAerg ic synaptic inputs. Such excitatory (Boudaba et al, 1997; Daftary et al, 1998; Cui et al, 2001) and inhibitory synaptic... Previous studies have suggested that brain stem noradrenergic inputs differentially modulate neurons in the paraventricular nucleus (PVN). Here, we compared the effects of norepinephrine (NE) on spontaneous GABAergic inhibitory postsynaptic currents (sIPSCs) in identified PVN neurons using slice patch technique. In 17 of 18 type I neurons, NE (30∼100μM) reversibly decreased sIPSC frequency to 41⁑7% of the baseline value (4.4⁑0.8 Hz, p＜0.001). This effect was blocked by yohimbine... -
Relatoinship between Sarcoplasmic Reticular Calcium Release and Na+-Ca2+ Exchange in the Rat Myocardial Contraction
Eun-GiKim, SoonJinKim, ChangMannKo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 14 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.3 4 197-210 (14 pages)
in the rat heart (Langer & Rich， 1992; Langer et al， 1995). In the compartment， Ca2+ may be trapped by the negatively charged phos pholipid in the subsarcolemmal space after it is re leased from SR. Therefore， a certain fraction of the Ca2+ is extruded out of the cell via Na + _Ca2+ exchange before it difuses to and interacts with the myofilament to trigger the contraction (Langer &... Suppressive role of Na-Ca2 exchange in myocardial tension generation was examined in the negative frequency-force relationship (FFR) of electric field stimulated left atria (LA) from postnatal developing rat heart and in the whole-cell clamped adult rat ventricular myocytes with high concentration of intracellular Ca2 buffer (14 mM EGTA). LA twitch amplitudes, which were suppressed by cyclopiazonic acid in a postnatal age-dependent manner, elicited frequency-dependent and... -
Different Mechanisms for K+-Induced Relaxation in Various Arteries
SukHyoSuh, SungJinPark, JaiYoungChoi, JaeHoonSim, YoungChulKim, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.4 6 415-425 (11 pages)
Newman， 1987). K+ re- ..PAGE:2 416 SH Suh el 허 leased by active neurons may be sufficient to cause K ' -induced relaxation of cerebral blood vessels， and this may be a factor linking cerebral blood f。、to local neuronal metabo1ism As the relaxation by the elevation of extracellular K + (K + -induced relaxation) is blocked by Ba2+ andj or ouabain and not by blockers of ATP-sensitive K +... [K]o can be increased under a variety of conditions including subarachnoid hemorrhage. The increase of [K]o in the range of 5∼15 mM may affect tensions of blood vessels and cause relaxation of agonist-induced precontracted vascular smooth muscle (K-induced relaxation). In this study, effect of the increase in extracellular K concentration on the agonist-induced contractions of various arteries including resistant arteries of rabbit was examined, using home-made...