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Comparison of allele-specific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle
Kozet Avanus, Ahmet Altınel 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 9 465-470 (6 pages)
was treated daily. The age of the transgenic mice at the start of KCHO-1 treatment was 13 weeks and KCHO-1 was administered daily for 4 weeks (mouse ages from 91 days to 119 days). Behavioral analysis Before KCHO-1 was delivered to mice, behavioral tests were performed. Prior to testing, the mice underwent a one-week training period to adapt to the apparatus (7650 Accelerating Model, Ugo... study was to compare sensitivity, specificity, positive and negative predictive values, accuracy, and rapidity of allele-specific polymerase chain reaction (AS-PCR), created restriction-site PCR (CRS-PCR), and PCR with primer-introduced restriction analysis (PCR-PIRA), three methods used in identification of CVM carriers in a Holstein cattle population. In order to screen for the G＞T mutation in the solute carrier family 35 member A3 (SLC35A3) gene, DNA sequencing as the gold standard method wa... -
Microarray Analysis of Gene Expression Profiles in Response to Treatment with Melatonin in Lipopolysaccharide Activated RAW 264.7 Cells
JuYeonBan, BumSikKim, SooCheolKim, DongHwanKim, Joo-HoChung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.1 4 23-30 (8 pages)
using a 24K cDNA micro array. MTT assay revealed that melatonin showed no cy totoxic effects in RAW 264.7 cells. Melatonin significantly inhibited LPS-induced NO production in RAW 264.7 cells. The results of the micro- array analysis showed that 221 7 genes were differentially expressed by RAW 264.7 cells following LPS treatment (1073 up-regulated and 1144 down-regulated). Melatonin plus LPS... conducted microarray analysis to evaluate the global gene expression profiles in response to treatment with melatonin in lipopolysaccharide (LPS) activated RAW 264.7 macrophage cells. In addition, eight genes were subjected to real-time reverse transcription polymerase chain reaction (RT-PCR) to confirm the results of the microarray. The cells were treated with LPS or melatonin plus LPS for 24 hr. LPS induced the up-regulation of 1073 genes and the down-regulation of 1144 genes when compared to... -
구강 외 노출시간에 따른 흰쥐 치아 치주인대세포 활성도의 MTT 검색법을 이용한 평가
전인수, 김의성, 김진, 이승종, Jeon. In-Soo, Kim. Eui-Seong, Kim. Jin, Lee. Seung-Jong 대한치과보존학회 大韓齒科保存學會誌 11 Pages
대한치과보존학회 大韓齒科保存學會誌 2006, Vol.31 No.5 398-408 (11 pages)
치아 자체를 MTT용액에 저장한 다음 흡광도 측정을 이용한 정량적 분석과 동시에 냉동 절단법을 이용하여 조직을 관찰하였다. MTT 검색에 의한 흡광도 값에 있어 즉시 처치군과 10분 건조군 사이에는 통계학적인 유의차가 없었다 (p > 0.05). 그러나, 즉시 처치군 및 10분 건조군은 20분과 40분 및 60분 건조군과 비교시 통계학적인 유의차를 보였다 (p <0.05). 또한, 20분 건조군도 40분 및 60분 건조군과의 군간 비교에서 각각 통계학적인 유의차를 보였다 (p<0.05). 그러나, 40분 건조군과 60분 건조군 사이에는 통계학적인... -
Effect of low intensity pulsed ultrasound in activating the mitogen-activated protein kinase signaling pathway and inhibition inflammation cytokine synthesis in chondrocytes
Eun-Jung Kim, Gye-Yeop Kim 물리치료재활과학회 Physical Therapy Rehabilitation Science 5 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2014, 제 3권 제 1호 6 33-37 (5 pages)
rat primary chon- drocyte [18]. Western blotting analysis For the western blotting analysis of the dish cultured for 1 week, cartilage tissues specimens were harvested 2 hours af- ter the last LIPUS and were cut into smaller pieces. Chondrocytes were pretreated with the p38 inhibitor ..PAGE:3 Kim and Kim: Effect of LIPUS in activating the MAPK and cytokine synthesis in chondrocytes 35 Figure 1.... -
The effects of therapeutic ultrasound stimulation on the inflammation cytokine in rat articular chondrocytes
Eun-Jung Kim, Su-jin Hwang, Gye-Yeop Kim 물리치료재활과학회 Physical Therapy Rehabilitation Science 6 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2013, 제 2권 제 1호 4 21-26 (6 pages)
was about 20 pg/ml, the linear assay range was between 100 and 2,000 pg/ml. Aliquots of 100 μl from control or LPS-treated cultures were analyzed according to the manufactures instruction. Data analysis Data analysis was performed with PASW Statistics 18.0 (IBM Co., Armonk, NY, USA). All of the data were ex- pressed as mean (SD). The difference between two mean values was analyzed by stude... Objective: The aim of this study was to investigate the effect of therapeutic ultrasound (US) of cell viability and inflammatory cytokine in rat articular chondrocyte cultures stimulated with lipopolysaccharide (LPS). Design: One group pretest-posttest design. Methods: Cultured chondrocytes were treated with US and/or LPS and assessed for viability, Tumor necrosis factor (TNF)-α and Interleukin (IL)-1 production. Results: Oxidative stress was induced in rat chondrocytes with LPS. The cell... -
The Extract of fermented antler activates mouse spleen cells
Hye Yeoul Yang, Hong Gu Joo 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 302 499-499 (1 pages)
of antler on immunity. Especially, we investigated in this study whether an extract of fermented antler may have immunomodulatory effects on spleen cells. Materials and Methods: We performed MTT assay, measuring biological activity and proliferation rate and FACS analysis for characterizing the subsets of spleen cells, including mainly lymphocytes. Results: After fermented antler extract... -
Evaluationof Alcohol induced Hepatic damages using 18F-Fludeoxyglucose Positron Emission Tomography and 99mTc-Tin Colloid Single hotonEmission Computed Tomography
Mun ki Kim, Sung A Kang 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 301 499-499 (1 pages)
of antler on immunity. Especially, we investigated in this study whether an extract of fermented antler may have immunomodulatory effects on spleen cells. Materials and Methods: We performed MTT assay, measuring biological activity and proliferation rate and FACS analysis for characterizing the subsets of spleen cells, including mainly lymphocytes. Results: After fermented antler extract... -
Exposure of cigarette smoke extract repressed cellviability of placental cells via regulation of cell cycle related genes in a placentacell model
Hae Miru Lee, Kyung Chul Choi 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 299 497-498 (2 pages)
RT-PCR analysis, weconfirmed that HB1.F3.CD cells expressed CD gene and HB1.F3.CD.IFN- β cells expressed both CD and IFN- β genes. A modified transwell migration assay showed th atHB1.F3.CD and HB1.F3. CD.IFN-β cells selectivelymigrated toward JEG-3 human choriocarcinoma cells. When co-cultured with HB1.F3. CDor HB1.F3.CD.IFN-β cells in the presence of5-FC, the viability of JEG-3 cells...