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Effects of Chlorhexidine digluconate on Rotational Rate of n-(9-Anthroyloxy)stearic acid in Model Membranes of Total Lipids Extracted from Porphyromonas gingivalis Outer Membranes
Hye-OckJang, Dong-WonKim, Byeong-IllKim, Hong-GuSim, Young-HoLee, Jong-HwaLee, Jung-HaBae, Moon-KyoungBae, Tae-HyukKwon, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.2 3 83-88 (6 pages)
it and the carboxyl group) (Tilley et al, 1979; Villala in & Prieto, 1991; Abrams et al, 1992; Effects of Chlorhexidine digluc onate on Rotational Rate of n-(9-Anthroyloxy)stearic acid in Model Membranes of Total Lipids Extracted from Porphyromonas gingivalis Outer Membranes Hye-Ock Jang, Dong-Won Kim, Byeo ng-Ill Kim, Hong-Gu Sim, Young-Ho Lee, Jong-Hwa Lee, Jung-Ha Bae 1 , Moon-Kyoung Bae 2... of chlorhexidine digluconate. Large unilamellar vesicles (OPGTL) were prepared with total lipids extracted from cultured Porphyromonas gingivalis outer membranes (OPG). The anthroyloxy probes were located at a graded series of depths inside a membrane, depending on its substitution position (n) in the aliphatic chain. Fluorescence polarization of n-(9-anthroyloxy)stearic acid was used to examine effects of chlorhexidine digluconate on differential rotational mobility, while changing the probes'... -
Effects of Chlorhexidine Digluconate on Rotational Rate of n-(9-Anthroyloxy)stearic Acid in Porphyromonas ginginvalis Outer Membranes
Hye-OckJang, Seong-KweonCha, ChangLee, Min-GakChoi, Sung-RyulHuh, Sang-HunShin, In-KyoChung, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 2 125-130 (6 pages)
of cell membranes may not be useful in building a hypothesis for the mechanism of pharmacological action of chlorhexidine on bacteriostatic or bactericidal effects. However, little attention has been given to the effects of chlorhexidine on the fluidity of special domain of anaerobic bacterial membranes. Furthermore, no studies have been carried out on the effects of chlorhexidine on the... 9-AS, 12-AS and 16-AP) probes, indicating different membrane fluidity. Chlorhexidine digluconate increased the rate of rotational mobility of hydrocarbon interior of the cultured Porphyromonas gingivalis outer membranes (OPG) in a dose-dependent manner, but decreased the mobility of surface region (membrane interface) of the OPG. Disordering or ordering effects of chlorhexidine digluconate on membrane lipids may be responsible for some, but not all of its bacteriostatic and bactericidal actions. -
Effects of Local Anesthetics on Rotational Mobility of n-(9-Anthroyloxy)stearic Acid in Neuronal Membranes
Hye-OckJang, ChangLee, Min-GakChoi, Sang-HunShin, In-KyoChung, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 1 119-124 (6 pages)
role in membrane permeability to sodium, calcium, and potassium (Green et al, 1980). Effects of local anesthetics on motion, order and phase transitions of bulk bilayer systems of native or model membranes have received cons iderable attention in past decades. This is due to the interest in biological membranes as well as the unique information on intermolecular interactions that can be deri... of 3-AS, 6-AS, 9-AS and 16-AP were different depending on depth of hydrocarbon interior. In a dose-dependent manner, tetracaine, bupivacaine, lidocaine, prilocaine and procaine decreased anisotropy of 3-AS, 6-AS, 9-AS and 16-AP in the hydrocarbon interior of the SPMV. These results indicate that local anesthetics have significant disordering effects on hydrocarbon interior of the SPMV, thus affecting the transport of Na and K in nerve membranes and leading to anesthetic action. -
Extracellular ATP Stimulates Na+ and CI- Transport through the Activation of Multiple Purinergic Receptors on the Apical and Basolateral Membranes in M-l Mouse Cortical Collecting Duct Cells
JinSupJung, SookMiHwang, RyangHwaLee, SooKyungKang, JaeSukWoo, YongKeunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.3 5 231-241 (11 pages)
Val 5 231 ←241， J“ 2αn Extracellular ATP Stimulates Na' and CI Transport through the Activation of Multiple Purnergc Receptors on the Apical and Bsolateral Membranes in M-l Mouse Corical Collecting Duct Cells Jiu Sup Juug1， Sook M Hwaug1， Raug H‘a rl， Soo Kuug Kaugl， Jae Suk Woo， aud Youg Keuu Km kι，ιh Cter f M이ι이ar Medi，le & De πeut o Phyiolog， Clkge o Mei야 Pιa Nau<"... of apical cystic fibrosis transmembrane regulator (CFTR) chloride channels and Ca2- activated chloride channels, but Na absorption was not mediated by activation of epithelal sodium channel (ENaC). ATP increased cAMP content in M-1 cells. The RT-PCR analysis demonstrated that M-1 cells express P2Y2, P2X3 and P2Y4 receptors. These results showed that ATP regulates Na and Cl transports via multiple P2 purinoceptors on the apical and basolateral membranes in M-1 cells. -
Changes in Phosphate Transporter Activity Evaluated by Phosphonoformic Acid Binding in Cadmium-Exposed Renal Brush-Border Membranes
JinMoChung, DoWhanAhn, KyoungRyongKim, YangSaengPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.5 8 513-519 (7 pages)
3: 513-519， October， 1999 Changes in Phosphate Transporter Activity Evaluated by Phospho noformic Acid Binding in Cadmium-Exposed Renal Brush-Border Membranes Jin Mo Chung， Do Whan Ahn， Kyoung Ryong Kim， and Yang Saeng Park Department of Physio1ogy， Kosin Medica1 College， Pusan 602-030， Korea Direct exposure of rena1 tubu1ar brush-border membranes (BBM) to free cadmium (Cd) causes a... Direct exposure of renal tubular brush-border membranes (BBM) to free cadmium (Cd) causes a reduction in phosphate (Pi) transport capacity. Biochemical mechanism of this reduction was investigated in the present study. Renal proximal tubular brush-border membrane vesicles (BBMV) were isolated from rabbit kidney outer cortex by Mg precipitation method. Vesicles were exposed to 50∼200 μM CdCl2 for 30 min, then the phosphate transporter activity was determined. The range of Cd concentration... -
Increased Activity of Large Conductance Ca2+-Activated K+ Channels in Negatively-Charged Lipid Membranes
JinBongParkPanDongRyu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.4 15 529-539 (11 pages)
and the differences were more pronounced at the lower ionic strength. The average slope conductances at 100 mM KCl were 251: 9.9， 360: 8.7 and 356: 12.4 (mean: SEM) pS in PE， PS and PI membranes respectively. The potentials at which Po was 1/2， appeared to have shifted left by 40 m V along voltage axis in the membranes formed with PS or PI. Such shif was consistent1y seen at pCa 5， 4.5， 4... times larger than those in PE membranes, and the differences were more pronounced at the lower ionic strength. The average slope conductances at 100 mM KCl were 251⁑9.9, 360⁑8.7 and 356⁑12.4 (mean⁑SEM) pS in PE, PS and PI membranes respectively. The potentials at which Po was 1/2, appeared to have shifted left by 40 mV along voltage axis in the membranes formed with PS or PI. Such shift was consistently seen at pCa 5, 4.5, 4 and 3.5. Estimation of the effect of surface... -
[3H]MK-801 Binding to the Synaptic Membranes of Rat Forebrains Age-related Regulation by Glutamate, Glycine and Spermine
JungsookCho, JaeYangKong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.2 1 117-125 (9 pages)
brief， synaptic membranes (300 μg of mem brane protein per tube) were incubated at 300C for 60 min in a final volume of 1 ml reaction mixture， containing 50 mM Tris-acetate buffer， pH 7.1， 5 n CH]MK-801， and appropriate concentrations of test ligands. The incubation was ternated and the bound radioactivity to the membranes was separated and measured as described (Cho et al， 1996).... the synaptic membranes of young and aged rat forebrains. The binding in the presence of glutamate and glycine increased dramatically with growth between 1 and 6 weeks old, and thereafter declined gradually with aging. Glutamate, glycine or spermine respectively increased the binding with growth. Glutamate maintained the binding during aging, while glycine or spermine significantly decreased the binding in the aged brain. The maximum stimulation by glycine varied depending on the ages of brains.... -
Rapid Measurement of NH3 and Weak Acid Permeation Through Liposomes and Renal Proximal Tubule Membranes
Bae, Hae-Rahn, Suh, Duck-Joon, Lee, Sang-Ho 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 11 203-214 (12 pages)
of nonelectrolytes through membranes with the advantages of instantaneous mixing effect, good resolution time and easy manipulation. Key Words: Membrane permeability, Weak acid, NH3, Stopped-flow technique Fluorescence INTRODUCTION The mechanism of passive permeation of non- electrolytes through the biological membranes has been studied for long period of time. In 1902, the significance of... of nonionized formic acid and initial rate of flux of formic acid in liposome, and the slope coincided with the value of permeability coefficient of formic acid measured in pH 7.0. These results show that techniques of stopped-flow and epifluorescence microscopy with entrapped fluorophore provide the precise method of measurement of very rapid transport of nonelectrolytes through membranes with the advantages of instantaneous mixing effect, good resolution time and easy manipulation. -
Contribution of Bulk Flow to Transport Mechanisms of the Membranes Surrounding Amniotic Fluid in the Rabbit
Lim, Young-Cheol, Lee, Sang-Jin, Sung, Ho-Kyung 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 1호 8 79-90 (12 pages)
results the following conclusions could be made: 1) There is no net movement of water or monovalent cations across the membranes surrounding amniotic fluid in isotonic isovolumetric condition. In contrast, there is a net efflux of amniotic fluid by osmotic bulk flow, resulting in elevation of Na+ concentration in hypotonic isovolumetric condition. 2) In hypervolumetric conditions, there is a... and decreased Censored group, the observations were identical to isovolumetric and decreased Censored group. From these results the following conclusions could be made: 1) There is no net movement of water or monovalent cations across the membranes surrounding amniotic fIuid in isotonic isovolumetric condition. In contrast, there is a net efflux of amniotic fluid by osmotic bulk flow, resulting in elevation of Na+ concentration in hypotonic isovolumetric condition. 2) In hypervolumetric... -
Actions of Ca Antagonists on the Voltage Dependent Ca Channel in Mammalian Smooth Muscle Cell Membranes
Kitamura, Kenji, Okie, Masahiro, Kuriyama, Hiroshi 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1989, 제 23권 제 1호 1 1-12 (12 pages)
Ca Channel in Mammalian Smooth Muscle Cell Membranes Kenji Kitamura, Masahiro Okie and Hiroshi Kuriyama Department of Pharmacology, Faculty of Medicine, Kyshu University, Fukuoka 812, Japan INTRODUCTION Intracellular free Ca concentrations play an essen- tial role in regulation of the contractions relaxation cycle in mammalian smooth muscle cells, e.g. with concentrations of Ca above 0. l -...