자료유형
등재정보
발행기관
- 한국식물병리학회(8)
- 대한생리학회-대한약리학회(4)
- 대한신경정신의학회(3)
- 대한기계학회(2)
- 대한설비공학회(2)
- 대한생리학회(1)
- 대한수의학회(1)
- 대한화학회(1)
- 한국습지학회(1)
- 한국웰니스학회(1)
- 한국전기화학회(1)
- 한방비만학회(1)
간행물
- THE PLANT PATHOLOGY JOURNAL (6)
- THE KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY(4)
- 신경정신의학(3)
- JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY(2)
- 설비공학논문집(2)
- 식물병연구(2)
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY(1)
- KOREAN JOURNAL OF VETERINARY RESEARCH(구 대한수의학회지)(1)
- 대한생리학회지(1)
- 대한한방비만학회지(1)
- 전기화학회지(1)
- 한국습지학회지(1)
- 한국웰니스학회지(1)
전체 선택해제
총 26건 검색
본문검색 결과만 보기
-
Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 1호 9 80-86 (7 pages)
PCR-amplifed probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specifc detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The sub- sequent MTH step using the PCR products as probes can replace... PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using... -
원례보저 ： 6-Hydroxydopamine 유발 SH-SY5Y 세포주 손상에 대한 resveratrol의 신경보호 효과
장건천, 김형춘, 위명복 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2014, 제 54권 제 1호 1 1-6 (6 pages)
× g에서 5분간 원심 분리하였다. 상등액을 버 린 다음 1% FBS를 함유한 DMEM으로 pipet을 이용하여 세포를 단일세포로 만들고 이것을 2×10 5/mL 농도가 되게 하였다. 100L를 microtube에 옮기고 Annexin V & Dead ..PAGE:3 Neuroprotective Effect of Resveratrol 3 Cell Kit(EMD Millipore, USA)를 이용하여 동량의 Annexin V & Dead Cell reagent를 첨가해 20분 동안 암실온 조건 에 방치한 후 세포분석기(Muse Cell Analyzer;... -
체감의이인탕(體減薏苡仁湯)이 비만유도 흰쥐의 체중,지방조직, 혈액변화, leptin과 Uncoupled protein에 미치는 영향
송재철(Jae Chul Song), 김길수(Kil Su Kim) 한방비만학회 대한한방비만학회지 25 Pages
한방비만학회 대한한방비만학회지 2001, 제 1권 제 1호 10 106-130 (25 pages)
수 거하여 4℃에서 12000rpm으로 15분간 원심분리한다. RNA층만 떠서 새로운 microtube에 옮기고 동량의 100% isopropanol을 혼합한 후 약 15 분 정도 상온에 두었다가 4℃에서 12000rpm으로 15분간 원심분리한다. 상층액을 제거하고 RNA pellet에 1.5㎖의 75% ethanol로 vortexing하여 세척한 후 4℃, 12000rpm으로 5분간 원 심분리한다. 5-10분간 상온에서 완전히 건조시킨 다음 100㎕의 DEPC-water에 RNA를 녹인 다음... -
Alteration of Striatal Tetrahydrobiopterin in Iron-Induced Unilateral Model of Parkinson’s Disease
BijayAryal, Jin-KooLee, , HakRimKim, , Hyung-GunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.2 6 129-134 (6 pages)
for 30 sec and cen- trifuged (8,000 rpm, 10 min) for extraction of DA and BH4 from striatum. The clear supernatant was collected in 100 μL of microtube and was dried under gentle stream of ni- trogen at 45 o C. The dried extract was reconstituted with 100 μL of 50% methanol containing 0.1% formic acid and 10 μL was injected into LC-MS/MS system. The sample was eluted with mobile phas e and... It has been suggested that transition metal ions such as iron can produce an oxidative injuries to nigrostriatal dopaminergic neurons, like Parkinson’s disease (PD) and subsequent compensative increase of tetrahydrobiopterin (BH4) during the disease progression induces the aggravation of dopaminergic neurodegeneration in striatum. It had been established that the direct administration of BH4 into neuron would induce the neuronal toxicity in vitro. To elucidate a role of BH4 in patho-genesis in... -
Effects of Nitric Oxide on Inhibitory Receptors of Rod Bipolar Cells of Rat Retina
No-GiPark, Sun-HoBai, Chang-SubJung, Myung-HoonChun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.6 6 347-352 (6 pages)
I4AA, imidazole-4-acetic acid; BIC, bicu- culline; PTX, picrotoxin; CACA, cis-4-aminocrotonic acid. ..PAGE:2 348 NG Park, et al 1 CaCl 2, 1 MgCl2 and 10 HEPES (pH 7.4). Subsequently, the microtube was shaken gently for mechanical dissocia- tion of the cells. The dissociated cells were placed on a lectin-coated coverslip and stored in a storage chamber filled with Ringer solution in a... The effects of nitric oxide (NO) on inhibitory neurotransmitter receptors and some types of inhibitory receptors in dissociated rod bipolar cell (RBC) were investigated. In the whole cell voltage-clamping mode, the gamma-aminobutyric acid (GABA) activated current showed both sustained and transient components. GABA activated transient current was fully blocked by bicuculine, a GABAA receptor antagonist. The cis-4-aminocrotonic acid (CACA), a GABAC receptor agonist, evoked the sustained current... -
Roles of Gonadal Steroids on Exocrine Secretion of Isolated Perfused Rat Pancreas
HyungSeoPark, SeHoonKim, HyoungJinPark, MeeYoungLee, YoungHeeHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.4 5 217-221 (5 pages)
flow of pancreatic juice was determined by measuring the length occupied by pancreatic juice, which was collected in microtube with a capacity of 3.8 μl/cm. The activity of α-amylase in pancreatic juice was measured using a previously reported method (Rick & Stegbauer, 1974). Statistical analysis of data All results are illustrated as means±S.E. The data were analyzed using the Student's... To clarify the roles of gonadal steroids on pancreatic exocrine secretion, effects of progesterone and estradiol-17β on spontaneous and secretagogue-induced exocrine response of isolated perfused rat pancreas were investigated. Intra-arterial infusion of progesterone resulted in significant increase of the spontaneous pancreatic fluid and amylase secretion dose-dependently. However, estradiol-17β did not exert any influence on spontaneous pancreatic exocrine secretion. Exogenous secretin,... -
Effects of γ-Aminobutyric Acid on Intrinsic Cholinergic Action in Exocrine Secretion of Isolated, Perfused Rat Pancreas
YongDeukPark, HyungSeoPark, ZhengYunCui, HyoungJinPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 8 169-174 (6 pages)
minutes before the acetylcholine in fusion until the end of the experiment. Measurements The fluid secretion of the isolated pancreas was deter- mined by measuring the length of microtube, which had a capacity of 3.8μl/cm, filled by pancreatic juice for 15 minutes (Park et al, 2000). The activity of α-amylase in pancreatic juice was measured according to the method described elswhere (Rick... γ-Aminobutyric acid (GABA) has been reported to enhance exocrine secretion evoked not only by secretagogues but also by intrinsic neuronal excitation in the pancreas. The pancreas contains cholinergic neurons abundantly that exert a stimulatory role in exocrine secretion. This study was undertaken to examine effects of GABA on an action of cholinergic neurons in exocrine secretion of the pancreas. Intrinsic neurons were excited by electrical field stimulation (EFS; 15 V, 2 msec, 8 Hz, 45 min)... -
Desipramine 장기투여가 백서 대뇌피질의 Adenylate Cyclase 의 활성도와 GABAb 및 Noradrenaline 수용체후 구조의 Coupling system 에 미치는 영향
金唤, 薫, , 橋本桓, 栗山欣彌 대한신경정신의학회 신경정신의학 18 Pages
대한신경정신의학회 신경정신의학 1994, 제 33권 제 3호 14 648-665 (18 pages)
각 농도의 반웅약물을 담은 lml용 microtube 에 빙냉상태하에 있던 조직균질액 50til을 가한 후 이를 진탕하여 37 °C에서 10분간 전배양하였다. 이에 최종농도 20mM theophylline 과 2mM isobutylmethyLxanthine(lBMX) 및 cAMP 생성계(최종농도 2mM ATP, 24mM crea tine phosphate, 200 units/ml 의 creatine phosphate) 과 최종농도 IOjiM 의 GTP가 함유된 반웅혼합액 (reaction mixture) 을 가하여, 총 lml의 배양액을... -
Haloperidol 장기투여로 유발된 백서 선조체의 Dopamine D2 수용체 초과민성에 대한 Lithium Carbonate 와 Bromocriptine의 영향
이중용, 김영훈, 장환일 대한신경정신의학회 신경정신의학 12 Pages
대한신경정신의학회 신경정신의학 1995, 제 34권 제 3호 24 895-906 (12 pages)
25jil가 들어있는 microtube에，빙냉상태의 조직 균질액 50인를 가하여 혼합시킨후 37°C에서 10분간 전배 양시킨 다음，반응 혼합액 25빈를 가하여 혼합후 37°C에서 10분간 배양하였다. 이후 끓는 물에 2분간 넣어 반응을 종결시켰으며，5분간 원심분리(16,000 rpm, Beckman microfuge, USA) 하여 상중액 70|il를 얻어 forskolin이 함유된 것은 4,000배, 나머지는 400배 희석하여 방사면역분석 키트(Amersham, England)을...