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Fluoroquinolone resistance mechanisms of Escherichia coli from swine feces in South Korea
Yoon Sung Hu, Yeon Soo Chung, Dae Ho Kim, Ji Min Jeon, Young Kyung Park, Sook Shin, Kun Taek Park, Yong Ho Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 189 435-436 (2 pages)
index asevidence of clinical signs [2]. The DIA was conducted as described previously [3]. Results: The DIA showed 11.17% higher sensitivity and 14.08% higher specificity than clinicalsign determination. Particularly, the DIA method was useful for measuring thetiter of IBV vaccines that showed no clear stunting sign but propagated in embryonatedc hicken eggs similar to the propagation of a... -
Prevalence and Enumeration of Campylobacter at Different Steps of Broiler Slaughterhouses in South Korea
Hyun Jung Park, Jin Hee Lee, Jong Soo Lim, Jung Whan Chon, Kun Ho Seo, Young Jo Kim, Eun Jeong Heo, Hyang Jin Yoon , Yeon Hwa Kim, Sung H 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 109 388-389 (2 pages)
0.74] in the milk ring test wh ich is a more common screening test in dairy herds in Korea. The result correlated well with the milk-ELISA test (kappa, 0.799; CI, 0.627 which indicated an adequate sensitivity (90.91%; CI, 58.72 99.77) and specificity (99.87%; CI, 99.68 99.97). Of the 15 positive herds in milk te sts, ten herds (66.67%) were positive in the individual serological tests (kappa,... -
Single-tubenested RT-PCR (STNPCR) for Rapid Differential Diagnosis without DNA Carryover Contamination of PRRSV
Hee Jung Kim, Choi Kyu Park, Eun Mi Kim, Sang Geon Yeo 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 74 176-177 (2 pages)
at 7 2℃.The amplified DNA products have visualized by agarose gel electrophoresis and Blue-illuminator (Neoscience, Korea). The sensitivity and specificity of the test were evaluated on individual and mixed virus RNA samples, and DNA carryover contamination prevention capability of the RT-PCR was confirmed by artificially contaminated samples with a previously amplified DNA. Results: The... -
One-Step Multiplex RT-PCR without Carryover Contamination for the Detection and Differentiation of Type 1, Type 2 and High Pathogenic PRRSV
Hee Jung Kim, Choi Kyu Park, Eun Mi Kim, Sang Geon Yeo 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 73 175-176 (2 pages)
at 7 2℃.The amplified DNA products have visualized by agarose gel electrophoresis and Blue-illuminator (Neoscience, Korea). The sensitivity and specificity of the test were evaluated on individual and mixed virus RNA samples, and DNA carryover contamination prevention capability of the RT-PCR was confirmed by artificially contaminated samples with a previously amplified DNA. Results: The... -
Effects of cetirizine in dogs with chronic atopic dermatitis: a randomized, double blind, placebo-controlled trial
Yun-Hsia Hsiao, Charles Chen, Ton Willemse 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 14 549-553 (5 pages)
sensitivity of 85% and a specificity of 79% to differentiate dogs with atopic dermatitis (AD) from those with chronic or recurrent pruritus without AD. Effects of cetirizine in dogs with chronic atopic dermatitis: a randomized, double blind, placebo-controlled trial Yun-Hsia Hsiao 1,*, Charles Chen 1 , Ton Willemse 2 1Asian Referral Center, Taipei 106, Taiwan 2 Faculty of Veterinary Medicine,... This study was conducted to evaluate the effects of cetirizine in dogs with atopic dermatitis (AD) while fulfilling Favrot’s diagnostic clinical criteria. Dogs received either 3 mg/kg cetirizine (n = 27), or a placebo (n = 23) orally once daily for 14 days in a randomized, double blind, placebo-controlled study, without concomitant medication. The effects were evaluated using a pruritus visual analog scale at the start (day 0) and at day 14. After 14 days, cetirizine clearly had no effect on... -
Evaluation of effects of olfactory and auditory stimulation on separation anxiety by salivary cortisol measurement in dogs
Yoon-Joo Shin, Nam-Shik Shin* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 4 153-158 (6 pages)
expanded range high sensitivity salivary cortisol EIA kit (Salimetrics), and the salivary cortisol concentrations were calculated as Statistical analysis The calculated salivary cortisol levels in the samples were analyzed using the SPSS software (ver. 21.0; SPSS, USA). The alpha value was set at 0.05 in all cases, and all analyses involved two-tailed tests. Differences between two groups at... a serious behavioral problem in dogs. In this study, salivary cortisol was studied to determine if the owner’s odor or voice could reduce SA in dogs. Twenty-eight dogs with SA were divided into three groups: group 1 (control), group 2 (with owner’s clothes during the separation period; SP) and group 3 (a recording of the owner’s voice was played during SP). The dog’s saliva was collected after the owner and their dog were in the experimental room for 5 min (PRE). The dog was then separated... -
Time-course changes in the expression levels of miR-122, -155, and -21 as markers of liver cell damage, inflammation, and regeneration in acetaminophen-induced liver injury in rats
Hyun-Kyu Park, Woori Jo, Hyun-Ji Choi, Sungwoong Jang, Jae-Eun Ryu, Hyo-Ju Lee, Hyojin Lee, Hyejin Kim, Eun-Sil Yu, Woo-Chan Son 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 6 45-51 (7 pages)
identify and validate biomarkers with improved sensitivity and hepatic specicity to assist in the clinical management of APAP overdose [3]. Because the time ..PAGE:5 Time-course changes of miRNA in liver injury model 49 www.vetsci.org Fig. 6. Time-course changes in miR-155 (A) and miR-21 (B) expression levels. miR-155 and miR-21 levels were similar between treatment and vehicle (veh) groups,... Drug-induced liver injury (DILI) is a significant threat to patient health and a major concern during drug development. Recently, multiple circulating microRNAs (miRNAs) have been reported to be potential biomarkers for DILI. To adapt and validate miRNAs for clinical use, we investigated the time-course changes in miR-122 expression levels in an acetaminophen-induced liver injury model in rats. In addition, miR-155 and miR-21 were evaluated as makers of inflammation and regeneration,... -
An overview of calf diarrhea - infectious etiology, diagnosis, and intervention
Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)
- High sensitivity and specificity - Quantification of target pathogen - Low sensitivity - Restriction by characteristics of cells used for viral production - Requirement of proper sample collection and handling for virus viability - Not applicable for cytotoxic specimens - Time-consuming and laborious - Requires a large number of virus particles in the samples - Low throughput - Need for skilled... Calf diarrhea is a commonly reported disease in young animals, and still a major cause of productivity and economic loss to cattle producers worldwide. In the report of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or... -
Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey
G?khan Inat*, Belgin Siriken 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 7 321-326 (6 pages)
the sensitivity of the method, diverse geographical ..PAGE:5 Immunomagnetic detection of Escherichia coli O157 and Escherichia coli O157:H7 325 origins of cattle, numbers of cattle, study design, number of herds and cattle, sex and age of cattle, season, abattoir conditions and treatment with antimicrobial substances during the process [6,7,39]. It has also been reported that the prevalence... This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected from Samsun Province of Turkey. A total of 200 samples collected from cattle carcasses and the rectal contents of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200... -
Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand
Tawatchai Pohuang, Niwat Chansiripornchai*, Achara Tawatsin, Jiroj Sasipreeyajan 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 7 219-223 (5 pages)
I could not be detected with the primer set and PCR method described by Gelb et al. [8]. To overcome this problem, the new primer sets were designed and the nested RT-PCR was developed in order to increase the sensitivity and specificity of the test used to detect IBV from the infected samples. Moreover, the region amplified by the outer primer covering the HVR of the S1 gene (nucleotide... Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and...