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Nailbed (subungual) neoplasms in small animals
Jae Hoon Kim 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 25 153-153 (1 pages)
their histologic nature, can erode into the distal phalanx of the digits. Primary nailbed tumors of all types are uncommon in dogs and rare in cats. Relative incidence of the different tumor types is difficult to estimate. Any expansile mass at this site can result in lameness, digital enlargement, replacement or lysis of the third phalanx, and in many cases, disruption of normal nail growth.... -
Effect of canine mesenchymal stromal cells overexpressing heme oxygenase-1 in spinal cord injury
Seung Hoon Lee, Yongsun Kim, Daeun Rhew, Ahyoung Kim, Kwang Rae Jo, Yongseok Yoon, Kyeung Uk Choi, Taeseong Jung, Wan Hee Kim, O 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 15 377-386 (10 pages)
reported previously [16,21,30], but most of them were related to neurotropic factors or growth factors, which were aimed at continuous secretion of the factors that stimulate axonal growth and replacement of neurons throughout the lesion area [6,17,30]. In this study, treatment with MSC-HO-1 was designed to be a means to manifest antioxidant and anti-inflammatory effects to prevent further... Heme oxygenase-1 (HO-1) is a stress-responsive enzyme that modulates the immune response and oxidative stress associated with spinal cord injury (SCI). This study aimed to investigate neuronal regeneration via transplantation of mesenchymal stromal cells (MSCs) overexpressing HO-1. Canine MSCs overexpressing HO-1 were generated by using a lentivirus packaging protocol. Eight beagle dogs with experimentally-induced SCI were divided into GFP-labeled MSC (MSC-GFP) and HO-1-overexpressing MSC... -
Inhibition by miR-410 facilitates direct retinal pigment epithelium differentiation of umbilical cord blood-derived mesenchymal stem cells
Soon Won Choi, Jae-Jun Kim, Min-Soo Seo, Sang-Bum Park, Tae-Hoon Shin, Ji-Hee Shin, Yoojin Seo, Hyung-Sik Kim, Kyung-Sun Kang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 8 59-65 (7 pages)
cell replacement therapy for vision restoration has recently been under investigation. Many studies of RPE generation are based on embryonic stem (ES) or induced pluripotent stem (iPS) cells. It has even been suggested that pluripotent stem cells can spontaneously differentiate into RPE cells, with RPE cells spontaneously differentiating from ES or iPS cells in humans [1]. Moreover, it was... Retinal pigment epithelium (RPE) is a major component of the eye. This highly specialized cell type facilitates maintenance of the visual system. Because RPE loss induces an irreversible visual impairment, RPE generation techniques have recently been investigated as a potential therapeutic approach to RPE degeneration. A microRNA-based technique is a new strategy for producing RPE cells from adult stem cell sources. Previously, we identified that antisense microRNA-410 (anti-miR-410) induces RPE... -
Reactive oxygen species-mediated unfolded protein response pathways in preimplantation embryos
Ihsan Ali, Syed Zahid Ali Shah, Yi Jin, Zhong-Shu Li, Obaid Ullah, Nan-Zhu Fang 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 1 1-9 (9 pages)
/CDC 28-related kinase activity is required for signaling from the ER to the nucleus. Cell 1993, 74, 743-756. 55. Mori K, Ogawa N, Kawahara T, Yanagi H, Yura T. mRNA splicing-mediated C- terminal replacement of transcription factor Hac1p is required for efficient activation of the unfolded protein response. Proc Natl Acad Sci U S A 2000, 97, 4660-4665. 56. Nakayama T, Noda Y, Goto Y, Mori T.... Excessive production of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress-mediated responses are critical to embryonic development in the challenging in vitro environment. ROS production increases during early embryonic development with the increase in protein requirements for cell survival and growth. The ER is a multifunctional cellular organelle responsible for protein folding, modification, and cellular homeostasis. ER stress is activated by a variety of factors including... -
Evaluation of a canine small intestinal submucosal xenograft and polypropylene mesh as bioscaffolds in an abdominal full-thickness resection model of growing rats
A-Jin Lee, Sung-Ho Lee, Wook-Hun Chung, Dae-Hyun Kim, Dai-Jung Chung, Sun Hee Do, Hwi-Yool Kim* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 9 175-184 (10 pages)
a suitable replacement for artificial biological scaffolds in small animals. Keywords: biological scaffold, canine small intestinal submucosa (canine SIS), inflammatory response, polypropylene mesh, tensile load test Introduction Abdominal wall defects may develop after traumatic injury, necrotizing infection, tumor resection, congenital defects (e.g., omphalocele), and ventral wall hernias... in the SIS group were increased, but the number of CD8+ T cells in this group declined more rapidly than that in rats treated with the mesh. In the SIS group, few adhesions and well-developed autologous abdominal muscle infiltration into the SIS collagen fibers were observed. No significant differences in the tensile load test results were found between the SIS and mesh groups at 24 weeks. Canine SIS may therefore be a suitable replacement for artificial biological scaffolds in small animals. -
Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle
Sangho Choi, Hee-Jong Woo*, Jeongmin Lee* 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 3 127-137 (11 pages)
(G234A, C555T, C576T, and C630T) were synonymous polymorphisms that have been previously described by Brunelle et al. [4]. None of these particular substitutions resulted in an amino acid replacement in the corresponding protein sequence. Two other SNPs, A467G and A669C, were non-synonymous. That is, the point mutation (G for A) at nucleotide 467 encoded Cys (TGT) instead of Tyr (TAT) at... Bovine spongiform encephalopathy (BSE) is one of the fatal neurodegenerative diseases known as transmissible spongiform encephalopathies (TSEs) caused by infectious prion proteins. Genetic variations correlated with susceptibility or resistance to TSE in humans and sheep have not been reported for bovine strains including those from Holstein, Jersey, and Japanese Black cattle. Here, we investigated bovine prion protein gene (PRNP) variations in Hanwoo cattle [Bos (B.) taurus coreanae], a native... -
Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid
Goo Jang*, So Gun Hong, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 12 83-89 (7 pages)
15. Kwun J, Chang K, Lim J, Lee E, Lee B, Kang S, Hwang W. Effects of exogenous hexoses on bovine in vitro fertilized and cloned embryo development: Improved blastocyst formation after glucose replacement with fructose in a ..PAGE:7 Viable cloned calves derived from embryo cultured in different media 89 serum-free culture medium. Mol Reprod Dev 2003, 65, 167-174. 16. Lim KT, Jang G, Ko KH,... Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence was monitored. After 7 days of culturing, the... -
Differential patterns of nestin and glial fibrillary acidic protein expression in mouse hippocampus during postnatal development
Joong-Sun Kim, Juhwan Kim, Yujin Kim, Miyoung Yang, Hyosun Jang, Sungwoon Kang, Jong-Choon Kim, Sung-Ho Kim, Taekyun Shin*, Chan 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 1 1-6 (6 pages)
neurogenesis continues throughout mammalian life, including in humans [13]. The hippocampus is the most vulnerable brain region (especially multipotent progenitor cells in the DG), and cell replacement follows cell death induced by various effectors [14,21,26]. Changes in IF gene expression occur at key steps in cell differentiation in the mammalian CNS [17,28]. Cells expressing nestin show... Intermediate filaments, including nestin and glial fibrillary acidic protein (GFAP), are important for the brain to accommodate neural activities and changes during development. The present study examined the temporal changes of nestin and GFAP protein levels in the postnatal development of the mouse hippocampus. Mouse hippocampi were sampled on postnatal day (PND) 1, 3, 6, 18, and 48. Western blot analysis showed that nestin expression was high at PND 1 and markedly decreased until PND 18.... -
A Serine12Stop mutation in PB1-F2 of the 2009 pandemic (H1N1) influenza A: a possible reason for its enhanced transmission and pathogenicity to humans
Muthannan A. Ramakrishnan, Marie R. Gramer, Sagar M. Goyal, Srinand Sreevatsan* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 12 349-351 (3 pages)
66 of PB1-F2 affects the pathogenicity of an H5N1 virus in mice. The N66S mutation contributed to the high pathogenicity of the 1918 pandemic A/Brevig Mission/18 virus, and its replacement (S66N) attenuated this virus in mice [2]. Therefore, PB1-F2 has been identified as a key virulence factor among influenza A viruses. Extensive amino acid sequence analysis of the 2009 (H1N1) influenza A... As the scientific community scrambles to define the ancestry and lineages of the eight segments of new pandemic H1N1 strain, we looked for unique genetic events in this virus's genome to explain the newly found enhanced virulence and transmissibility among humans. Genome annotations of this virus identified a stop mutation replacing serine at codon 12 (S12Stop) of the PB1-F2 protein, a virulence factor in influenza A viruses. Here, we discuss the significance of this finding and how it may... -
Imaging evaluation of the liver using multi-detector row computed tomography in micropigs as potential living liver donors
Jung Min Ryu, Dong Hyun Kim, Min Young Lee, Sang Hun Lee, Jae Hong Park, Seung Pil Yun, Min Woo Jang, Seong Hwan Kim, Gyu Jin Rh 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 1 93-98 (6 pages)
potential living donor, the results of this study showed that the hepatic volume and vascular anatomy of the micropigs appeared to be sufficient for adequate replacement of the human liver. In conclusion, MDCT was a reliable imaging method for the evaluation of the liver and its vascular anatomy for xenotransplantation using micropigs. Acknowledgments This work was supported by a grant (code... The shortage of organ donors has stimulated interest in the possibility of using animal organs for transplantation into humans. In addition, pigs are now considered to be the most likely source animals for human xenotransplantation because of their advantages over non-human primates. However, the appropriate standard values for estimations of the liver of micropigs have not been established. The determination of standard values for the micropig liver using multi-detector row computed tomography...