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Development of indirect ELISA for the detection of antibodies to Japanese encephalitis virus in swine sera
Hyun Ye Jo, Dong Kun Yang, Ha Hyun Kim, Sang Ho Jang, In Soo Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 31 99-100 (2 pages)
of America, Australia and Japan. Serum virus neutralization test: Neutralizing antibodies to GETV were detected and quantified in 240 horse serum samples by using serum virus neutralization test (SN). Preparation of GETV antigen: Viruses were concentrated as described previously. In brief, the viral suspensions were filtered using membrane filters of 0.2 μm pore size to remove unwanted cell... -
Identification of determinants that mediate binding between Tembusu virus and the cellular receptor heat shock protein A9
Dongmin Zhao, Qingtao Liu, Xinmei Huang, Huili Wang, Kaikai Han, Jing Yang, Keran Bi, Yuzhuo Liu, Lijiao Zhang, Yin Li 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 8 528-535 (8 pages)
of DI and contains several epitopes that can induce neutralizing antibody [5]. As an enveloped virus, the first step in cell entry of Flavivirus involves binding of the E protein to a cellular receptor. Crystallographic study has revealed that the interaction between Dengue virus and dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) is ..PAGE:2 TMUV E... Heat shock protein A9 (HSPA9), a member of the heat shock protein family, is a putative receptor for Tembusu virus (TMUV). By using Western blot and co-immunoprecipitation assays, E protein domains I and II were identified as the functional domains that facilitate HSPA9 binding. Twenty-five overlapping peptides covering domain I and domain II sequences were synthesized and analyzed by using an HSPA9 binding assay. Two peptides showed the capability of binding to HSPA9. Dot blot assay of... -
Toward the development of a one-dose classical swine fever subunit vaccine
Rachel F. Madera, Lihua Wang, Wenjie Gong, Yulia Burakova, Sterling Buist, Jerome Nietfeld, Jamie Henningson, Ada G. Cino-Ozuna, Changch 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 9 393-405 (13 pages)
5 anti-CSFV neutralizing bodies that neutralized 50% [ND 50] of virus at 6 DPC and 30 ND50 at 9 DPC). In contrast, no E2-specific antibodies were detected by ..PAGE:9 One-dose CSF subunit vaccine 401 www.vetsci.org Table 2. As early as two weeks after KNB-E2 vaccination, pigs developed protective titers of anti-CSFV-neutralizing antibodies after virus challenge (14 DPC) Weeks post-vaccination... Highly contagious classical swine fever (CSF) remains a major trade and health problem in the pig industry, resulting in large economic losses worldwide. In CSF-endemic countries, attenuated CSF virus (CSFV) vaccines have been routinely used to control the disease. However, eradication of CSFV in a geographical area would require permanent reduction to zero presence of the virus. It is therefore of paramount importance to develop a safe, potent, and non-infectious CSF vaccine. We have previously... -
Microglial involvement in the development of olfactory dysfunction
Yoojin Seo, Hyung-Sik Kim, Kyung-Sun Kang 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 1 319-330 (12 pages)
Cx3cl1 neutralizing antibody or by the cathepsin S inhibitor LHVS successfully prevents microglial activation and, in turn, restores olfaction o f NPC mice. This illustration is a modified version of the ‘The Table of Contents Image’ in our recently published article as stated. Source: Seo Y, et al. Glia 2016, 64(12), 2291-2305. mediates neuronal-microglial communication through binding to... Olfactory impairment is the most common clinical manifestation among the elderly, and its prevalence increases sharply with age. Notably, growing evidence has shown that olfactory dysfunction is the first sign of neurodegeneration, indicating the importance of olfactory assessment an early marker in the diagnosis of neurological disorders. In this review, we describe the nature of olfactory dysfunction and the advantage of using animal models in olfaction study, and we include a brief... -
Efficacy evaluation of commercial disinfectants by using Salmonella enterica serovar Typhimurium as a test organism
Yangho Jang, Kwangjick Lee, Seonjong Yun, Myoungheon Lee, Jaeyoung Song, Byungjoon Chang, Nong-hoon Choe 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 11 209-216 (8 pages)
9 mL of Dey-Engley neutralizing solution (Difco, USA) for 5 min. The number of residual viable organisms was determined by determining plate counts on 3M Petrifilm Aerobic Count Plates (3M, USA). The concentration establishment test was performed in the same way as the suspension test, except that the contact times used were 30 sec, 1 min, and 5 min. Carrier tests Equivalent volumes of 3%... The efficacies of six commercial disinfectants were evaluated by using Salmonella enterica serovar Typhimurium under simulated natural conditions such as sub-zero temperature, short disinfecting time, and surface type (uneven or smooth). We used a suspensionmodel test to determine the disinfecting efficacy under varying contact times (1, 5, 10, and 30 min) and temperatures (25°C, 4°C, 0°C, and -10°C). The bactericidal effect according to surface structure was measured by using a carriermodel... -
Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice
Yu-Ying Liu, Wen-Tao Yang, Shao-Hua Shi, Ya-Jie Li, Liang Zhao, Chun-Wei Shi, Fang-Yu Zhou, Yan-Long Jiang, Jing-Tao Hu, Wei Gu, 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 5 159-167 (9 pages)
immunological area of GPV, containing an immunodominant region that can participate in neutralizing the virus [5]. Therefore, the VP2 protein may be a suitable target for research strategies aimed at developing new prophylactic vaccines against GPV. Lactobacillus is a microaerophilic, rod-shaped, Gram-positive, facultative anaerobic bacterium [37]. Lactobacillus has been demonstrated to have... strain, and the ratios and numbers of CD11c, CD3CD4, CD3CD8, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework ... -
Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
Marina G. Ferreira, Clara G. Duarte, Maira S. Oliveira, Karen L. P. Castro, Ma?lson S. Teixeira, L?lian P. G. Reis, Jos? A. Zamb 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 5 467-477 (11 pages)
of severe scorpion envenomation in South Tunisia: multivariate analysis of 951 cases. Toxicon 2008, 52, 918-926. 4. Chavez-Olortegui C, Kalapothakis E, Ferreira AMBM, Ferreira AP, Diniz CR. Neutralizing capacity of antibodies elicited by a non-toxic protein purified from the venom of the scorpion Tityus serrulatus. Toxicon 1997, 35, 213-221. 5. Chippaux JP, Goyffon M. Venoms, antivenoms, and... Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with... -
Use of laparoscopy for diagnosing experimentally induced acute pancreatitis in dogs
Hyun-wook Kim, Ye-in Oh, Ji-hye Choi, Dae-yong Kim, Hwa-young Youn, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 16 551-556 (6 pages)
the protruding (P) domain of the HEV capsid protein [9]. Even though we did not observed the presence of neutralizing antibodies, VLPs generated in our study contained the P domain that included amino acids 456-606. Therefore, we expect the antibodies produced by immunization with the swine HEV VLPs would include neutralizing or NOB antibodies. Exposure to the swine HEV VLPs also induced a... Diagnosis of acute pancreatitis in dogs remains a significant challenge despite the development of advanced diagnostic methodologies. Visual inspection and pancreas biopsy using laparoscopy are generally considered to be procedures free of complications when conducted on healthy animals. However, the usefulness of laparoscopy for diagnosing acute pancreatitis has not been assessed. In the present study, the efficacy of laparoscopy for diagnosing acute pancreatitis in dogs was evaluated in... -
Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors
Andrea Maria Blanc, Mabel Beatriz Berois, Lorena Magalí Tomé, Alberto L. Epstein, Juan Ramón Arbiza* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 9 59-65 (7 pages)
no neutralizing activity. This is in agreement with a recent study in which rabbits were immunized with a bovine herpesvirus 4 vector expressing BHV-1 gD ectodomain and bovine viral diarrhoea virus (BVDV) glycoprotein E2 ectodomain [4]. The rabbits in this investigation produced neutralizing antibodies against BVDV but not BHV-1 [4]. This response may also be related to the route of immu-... Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected... -
Alleviation of ascorbic acid-induced gastric high acidity by calcium ascorbate in vitro and in vivo
Joon-KyungLee, Sang-HyukJung, Sang-EunLee, Joo-HuiHan, EunjiJo, Hyun-SooPark, Kyung-SunHeo, DeasunKim, Jeong-SookPark, Chang-SeonMyung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.1 4 35-42 (8 pages)
The AUC 0-24h values of calcium ascorbate were greater than ascorbic acid with significant difference (p<0.05). The reason for the increased ab- sorption of calcium ascorbate was likely due to the neutralizing effect of the calcium salt. However, the AUC inf values were not sig- nificantly different in those two groups. Since the general dosage regimen of ascobate preparation is once daily and... Ascorbic acid is one of the most well-known nutritional supplement and antioxidant found in fruits and vegetables. Calcium ascorbate has been developed to mitigate the gastric irritation caused by the acidity of ascorbic acid. The aim of this study was to compare calcium ascorbate and ascorbic acid, focusing on their antioxidant activity and effects on gastric juice pH, total acid output, and pepsin secretion in an in vivo rat model, as well as pharmacokinetic parameters. Calcium ascorbate and...