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A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle
Hidetoshi Higuchi, Hidetomo Iwano, Kazuhiro Kawai, Takehiro Ohta, Tetsu Obayashi, Kazuhiko Hirose, Nobuhiko Ito, Hiroshi Yokota, 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 13 191-194 (4 pages)
PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit... (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be about 2.5 × 10 cfu/mL and was similar... -
Comparison of allele-specific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle
Kozet Avanus, Ahmet Altınel 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 9 465-470 (6 pages)
mice used in this study have an average lifespan of 128.9 ± 9.1 days. For selection of hSOD1 G93A Tg mice by genotyping, DNA was extracted from the tail of the mice, and a PCR assay was performed. All mice were housed at a constant temperature of 21 oC to 23 oC and humidity of 50% to 60% under a 12 h light/dark cycle. Female hSOD1 G93A Tg mice were colonized per experimental group (n =... of carriers and the mutant allele frequency were 3.2% and 0.016, respectively, among Holstein cattle in the Thrace region of Turkey. Among the three methods, the fastest but least accurate was AS-PCR. Although the rapidity of CRS-PCR and PCR-PIRA were nearly equal, the accuracy of PCR-PIRA was higher than that of CRS-PCR. Therefore, among the three methods, PCR-PIRA appears to be the most efficacious for screening of mutant alleles when identifying CVM carriers in a Holstein cattle population. -
Screening of Mycobacterium bovis in herd environmental samples by nested-PCR for rapid identification
Tae Woon Kim, Yunho Jang, Yu Sin Ok, Min Kyu Jeong, Bang Hun Hyun, Jae Myung Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 76 246-247 (2 pages)
of the commonly used PCR method. Conclusions: The sensitivity and specificity of two methods based on the polymerasechain reaction (PCR) for detection of M. bovis were compared. In the standard PCR method, the sensitivity of detection of M. bovis was very low due to contaminants and other microorganisms in environmental samples. However, the sensitivity of this nested-PCR for M. bovis was... -
Rapaid Screening of Apple mosaic virus in Cultivated Apples by RT-PCR
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2003, 19권 3호 7 159-161 (3 pages)
..PAGE:1 ..PAGE:2 ..PAGE:3 No PCR product was observed when Cucumber mosaic virus (Cucumovirus) or a crude extract of healthy apple was used as a template in RT-PCR with the same primers. The PCR product (669 bp) of the CP gene of the virus was cloned into the plasmid vector and resultant recombinant (pAPCP1) was selected for molecule of apple transformation to breed virus-resistant transgenic apple plants as the next step. This method can be useful for early stage screening of in vitro plantlet and genetic resources of... -
Screening of the Dominant Rice Blast Resistance Genes with PCR-based SNP and CAPS Marker in Aromatic Rice Germplasm
Kim. Jeong-Soon, Ahn. Sang-Nag, Hong. Sung-Jun, Kwon. Jin-Hyeuk, Kim. Yeong-Ki, Jee. Hyeong-Jin, Shim. Chang-Ki 한국작물학회 Korean journal of crop science 13 Pages
한국작물학회 Korean journal of crop science 2011, Vol.56 No.4 329-341 (13 pages)
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Single-tubenested RT-PCR (STNPCR) for Rapid Differential Diagnosis without DNA Carryover Contamination of PRRSV
Hee Jung Kim, Choi Kyu Park, Eun Mi Kim, Sang Geon Yeo 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 74 176-177 (2 pages)
UDG system and single tube reaction In conclusion, STN-RT-PCR with UDG system developed in the study could be applicable to detect and differentiate of PRRSVs (type 1 and type 2) in swine clinical laboratories. References [1] Wernike K, et al. 2013. Single-Tube Multiplexed Molecular Detection of Endemic Porcine Viruses in Combination with Background Screening for Transboundary Diseases. J... -
Development of Multiplex PCR for the Rapid Detection of Six Honeybee Viral Disease
Ha Na Jung, Mi Sun Yoo, Young Ha Kim, Hee Soo Lee, Seung won Kang 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 70 174-174 (1 pages)
RT-PCR. Frequently simultaneous infections with different viruses are diagnosed in seemingly healthy bee colonies. Therefore we developed a multiplex RT-PCR assay for the simultaneous detection of multiple bee viruses. Materials and Methods: Bee samples used for virus screening were collected from all over the country. Viral RNA was extracted using viral gene-spin viral DNA/RNA extraction... -
A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats
Su Hwa Lee, Byeong Yeal Jung, Nabin Rayamahji, Hee Soo Lee, Woo Jin Jeon, Kang Seuk Choi, Chang Hee Kweon, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 7 43-51 (9 pages)
Improved diagnostic and real-time PCR in rapid screening for Salmonella in the poultry food chain. Acta Vet Hung 2006, 54, 297-312. 30. Tan W, Shelef LA. Automated detection of Salmonella spp. in foods. J Microbiol Methods 1999, 37, 87-91. 31. Trkov M, Avgu tin G. An improved 16S rRNA based PCR method for the specific detection of Salmonella enterica. Int J Food Microbiol 2003, 80, 67-75. 32.... the multiplex real-time PCR assay, using 128 pure-cultured bacteria including 110 Salmonella isolates and 18 non- Salmonella isolates, was 100%, 100% and 99.1% for Salmonella spp., S. Typhimurium and S. Enteritidis, respectively. The sensitivity was 100%, 100% and 91.7% for Salmonella spp., S. Typhimurium and S. Enteritidis, respectively. The multiplex real-time PCR assay developed in this study could detect up to 0.54 ± 0.09 and 0.65 ± 0.07 log10 CFU/ml for S. Typhimurium and S. Enteritidis... -
Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene
Seung-Ok Lee, Dong-Kyeun Na, Chul-Min Kim, Ying-Hua Li, Yoon-Hee Cho, Jin-Ho Park, John-Hwa Lee, Seong-Kug Eo, Terry A. Klein, J 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 10 151-155 (5 pages)
16S rRNA was amplified from 4.2% (26/611) tick samples (Fig. 1). All the tick samples that tested positive to E. chaffeensis originated from Gyeonggi province (Table 1). The 396 bp PCR product Table 1. PCR screening of Haemaphysalis longicornis ticks collected from different provinces of Korea Province/Place* Number of ticks PCR positive Ehrlichia and/or Anaplasma spp. E. chaffeensis Gangwon 10... to screening by genus-specific (Ehrlichia spp. or Anaplasma spp.) real-time TaqMan PCR and speciesspecific (E. chaffeensis) nested-PCR based on amplification of 16S rRNA gene fragments. In all, 611 (47.4%) ticks tested positive for genus-specific amplification of 116 bp fragment of 16S rRNA of Ehrlichia spp. or Anaplasma spp. Subsequently, 396 bp E. chaffeensis-specific fragment of 16S rRNA was amplified from 4.2% (26/611) tick samples. The comparison of the nucleotide sequence of 16S rRNA gene...