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Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides
Hong-tao Chang, Xiu-yuan He, Yu-Feng Liu, Lu Chen*, Quan-hai Guo, Qiu-ying Yu, Jun Zhao, Xin-wei Wang, Xia Yang, Chuan-qing Wang 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 8 399-407 (9 pages)
(PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in... (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG... -
Genetic characterization of porcine circovirus - 2 field isolates from PMWS pigs
Jin H. Kim, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 6 31-39 (9 pages)
PCV-2, a putative major causative agent of PMWS, were isolated and genetically characterized. Viral genome of two field isolates (PC201DJ and PC201SS) from pigs showing typical PMWS was sequenced. The nucleotide sequence homology with other PCV-2 isolates was ranging from 95% to 99% in complete viral genomic sequence. The highly conserved nonanucleotide motif of replication origin was identical to... result indicates that although the genome of PCV-2 is relatively stable in general, minor genetic variations exist among PCV-2 isolates from the different geographic locations. These differences of viral genome might have an important implication for genetic characteristics of PCV-2 infection. Three major immunorelevant epitopes of capsid protein showed variations in amino acid sequences. Also, the variance of amino acid sequence in antigenic epitope existed between two Korean PCV-2 isolates. -
Porcine circovirus type 2 increases interleukin-1beta and interleukin-10 production via the MyD88-NF-kappa B signaling pathway in porcine alveolar macrophages in vitro
Junyuan Han, Shuxia Zhang, Yaqun Zhang, Mengmeng Chen, Yingjun Lv 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 8 183-191 (9 pages)
group at 6, 12, 24, and 48 h post-infection (hpi) were higher than those in the control group (p 0.05). Moreover, the levels of IL-1 reached a peak at 12 hpi and then decreased in the PCV-2-infected groups. The levels of IL-10 in the PCV2-infected group at 12, 24, and 48 hpi were also significantly higher than those in the control group (p 0.01), and the levels of IL-10 increased with the... Porcine alveolar macrophages (PAMs) represent the first line of defense in the porcine lung after infection with porcine circovirus type 2 (PCV2) via the respiratory tract. However, PCV2 infection impairs the microbicidal capability of PAMs and alters cytokine production and/or secretion. At present, the reason for the imbalance of cytokines has not been fully elucidated, and the regulatory mechanisms involved are unclear. In this study, we investigated the expression levels and regulation of... -
돼지 써코바이러스 2형 유전형 분류를 위한 nested-PCR 적용
추금숙, Chu. Keum-Suk 한국가축위생학회 韓國家畜衛生學會誌 6 Pages
한국가축위생학회 韓國家畜衛生學會誌 2011, Vol.34 No.1 13-18 (6 pages)
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전북 익산지역 양돈장에서 돼지생식기호흡기 증후군, 써코바이러스-2 및 마이코프라즈마 폐렴의 항체가 조사
추금숙, 형상기, 이지영, 김지영, 서이원, 정병우, Chu. Keun-Suk, Hyong. Sang-Gi, Lee. Ji-Young, Kim. Ji-Young, Seo. Lee-Weon, Jung. Bung-Woo 한국가축위생학회 韓國家畜衛生學會誌 8 Pages
한국가축위생학회 韓國家畜衛生學會誌 2007, Vol.30 No.3 305-312 (8 pages)
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Loop-mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Porcine Circovirus 2
Eun Mi Kim, Hyo Sung Jeon, Hee Jung Kim, Sang Geon Yeo, Choi Kyu Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 177 230-231 (2 pages)
..PAGE:1 230 Korean Journal of Veterinary Science the expression of transporting genes for cations. Materials and Methods: We used a human trophoblast cell line (BeWo cells) to test the impact of EDCs during pregnancy. We used well-known EDCs and applied different doses of octyl-phenol(OP; 10 , 10 , and 10 M), nonyl-phenol (NP;10 , 10 , and 10 M), and bisphenol A (BPA;10 , 10 , and 10... -
The Current Status of Swine Respiratory Diseases in South Korea: Porcine Respiratory and Reproductive Viruses (PRRSVs) and Porcine Circovirus Type 2 (PCV)
Ga Eun Lee, Jung Ah Kim, Chan Hee Lee, Hee Chun Chung, Hye Jung Yang, Bong Kyun Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 53 111-112 (2 pages)
..PAGE:1 Vol. 56, No. 1 Supplement 111 Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea, 23vision Corporation, 9 Macheon-ro 17-gil, Songpa-gu, Seoul 05723, Republic of Korea, 3Asia Animal Medical Institute, 224 Eunhaengnamu-ro, Hwaseong-si, Gyeonggi-do 18633, Republic of Korea Introduction: Infrared... -
Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays
In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)
..PAGE:1 JOURNAL OF Veterinary Science J. Vet. Sci.(2002), 3(3), 207-212 Abstract10) Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regul- ations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recom- binant porcine IL-2 (rpIL-2) was expressed in Escherichia coli . The size of rpIL-2 without signal peptides was... Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by... -
Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV
Sang-ho Cha , Carey Bandaranayaka-mudiyanselage , Chandima B. Bandaranayaka-mudiyanselage , Dharani Ajiththos , Kyoung-jin 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2018, 제 58권 제 1호 2 9-16 (8 pages)
..PAGE:1 pISSN 2466-1384 eISSN 2466-1392 大韓獸醫學會誌 (2018) 第 58 卷 第 1 號 Korean J Vet Res(2018) 58(1) : 9~16 https://doi.org/10.14405/kjvr.2018.58.1.9 9 Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV Sang-Ho Cha 1, Carey... -
구강액을 이용한 양돈장의 Porcine circovirus-2 감염에 대한 모니터링
김원일, Kim. Won-Il 한국임상수의학회 Journal of veterinary clinics 9 Pages
한국임상수의학회 Journal of veterinary clinics 2010, Vol.27 No.6 704-712 (9 pages)
Porcine circovirus-2 (PCV2) 는 돼지에서 여러 형태의 질병과 증후군의 발생과 관련이 되어있어 현재는 PCV-associated diseases (PCVAD)로 총괄적으로 분류된다. PCVAD에 의한 높은 경제적 손실 때문에 많은 양돈장들이 PCV2의 감염을 확인하기 위하여 혈청을 검사하고 있다. 하지만, 기존의 혈액채취법은 비용이 높고 많은 인력이 소요되므로 큰 규모의 병원체 검사에는 어려움이 있었다. 이에 본 연구에서는 혈액채취법을 이용한 돈군의 PCV2 검사법에 대한 대체방법으로 돈방 단위의 구강액채취법의 유용성을 실제 농장에서...