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Evaluation of Ash Deposition Behavior and Fine Particle Formation from Combustion of Low-rank Coal Impregnated with Molasses in DTF
Zi-Xiang Xu, Joo-Chang Park, Hyung-Taek Kim 한국열환경공학회 열환경공학 9 Pages
한국열환경공학회 열환경공학 2019, 제 14권 2호 6 40-48 (9 pages)
Ca and Fe. Through DTF fouling deposition experiement and fine particle formation experiment, correlation between AAEMs and fouling and fine particle formation was confirmed. To control these problems, introduction of various additives can be considered. Particularly, aluminum-silicon additives, which are known to reduce fouling and capture fine particles, are suitable because biomass has... a series of air pollutions. Thus, the investigation of ash deposition and fine particle formation is a significant topic to promote heat exchanger efficiency and protect the environment. In this study, three fuels, Berau coal, Berau coal impregnate with 10wt% and 20wt% molasses were selected. Experiments are carried out in drop tube furnace (DTF) under the combustion temperature of 1300°C and the ash probe temperature of 800°C with access air. The fuel is fed at a rate of 0.45g/min for 10min.... -
Rapidantigenic detection for European and North American genotype of PRRSV by lateral flow immunochromatographic assay
In Ohk Ouh, Kwang Myun Cheong, Kyung Min Park, Joon Bae Kim, Jung Hwa Kim, Ji Eun Yu, Se Eun Choe, Jienny Lee, Seung min Song, Jae Young 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 213 449-450 (2 pages)
142 and 163 position to suppress 3C protease activity based on infectious O1 Manisa cDNA. A point mutated 3ABC gene and VPO were amplified and sequenced to confirm the corrected sequence. These genes were ligated with pLEX and Topo vector for generating the transduced celllines from BHK21, ZZR, IBRS-2, LFBK, BHK-T7-9 cell. Western bot, penside kit and fluorescene assay were used to confirm... -
Isolation and characterization of a new porcine epidemic diarrhea virus variant that occurred in Korea in 2014
Dong-Kun Yang, Ha-Hyun Kim, Seung-Heon Lee, Soon-Seek Yoon, Jung-Won Park, In-Soo Cho 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 10 71-78 (8 pages)
1; G2a, genotype 2a; G2b, genotype 2b. Table 2. Amino acid (aa) variations in structural proteins during serial passaging of the PEDV QIAP1401 strain in Vero cells No. of passage Gene S ORF3 M N aa position 102 214 265 511 635 679 887 99 206 79 208 369 P3 Pro Met Asp Ser Leu Asp Ser Ser Ilo Ile Ala Ser P20 Ser Met Gly Phe Phe Glu Arg Cys Leu Ile Ser Leu P40 Ser Met Gly Phe Phe Glu Arg Cys Leu Ile... Outbreaks of porcine epidemic diarrhea (PED) have resulted in significant economic losses in the swine industry, and another PED outbreak occurred in 2014 in Korea. Isolating and culturing PED virus (PEDV) allow investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated two PEDV isolates (QIAP1401 and QIAP1402) from naturally infected piglets at Jeju-do, Korea. Viral propagation was confirmed in Vero cells based on... -
Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 7 307-316 (10 pages)
as a substrate. Color development was stopped with 2 M H2SO4, and the OD value at 450 nm was read on a spectrophotometer. Conjugation of antigen with colloidal gold Colloidal gold with an average particle diameter of approximately 20 to 25 nm was obtained by reduction of a HAuCl 4 solution with 1% trisodium citrate. Three milliliters of 1% trisodium citrate (w/v) was added to 100 mL of 0.01%... a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be... -
Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus
Eun-Ju Kim, Kwang-Myun Cheong, Ha-Kyung Joung, Bo-Hye Kim, Jae-Young Song, In-Soo Cho, Kyoung-Ki Lee, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 6 479-487 (9 pages)
Biologicals, USA) were diluted with 0.5% sucrose (Sigma) in 2.5 mM PBS (pH 7.0) to obtain final concentrations of 1 mg/mL and 2 mg/mL, respectively. Anti-rabbit IgG was then immobilized in the control line position and MAb 12F12 was immobilized in the test line position on a nitrocellulose membrane (Millipore, Germany) attached to a backing card (PJ Company, Korea). After the membrane was... Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identification of infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assay that employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 was expressed in E. coli, and MAbs against recombinant BLV gp51 were generated for the... -
An overview of calf diarrhea - infectious etiology, diagnosis, and intervention
Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)
such as bacteria, virus, hormones, drugs, and serum protein [105]. Latex particles are made of synthetic rubber and emulsified as billions of micelles of the same size with a desired diameter. Particle size typically ranges between 0.05 to 2 μm in diameter, and the presence of sulfate ions provides an inherent negative surface charge to the particles [109]. The prepared latex particles c... or outcomes. The multifactorial nature of calf diarrhea makes this disease hard to control effectively in modern cow-calf operations. The purpose of this review is to provide a better understanding of a) the ecology and pathogenesis of well-known and potential bovine enteric pathogens implicated in calf diarrhea, b) describe diagnostic tests used to detect various enteric pathogens along with their pros and cons, and c) propose improved intervention strategies for treating calf diarrhea. -
Molecular characterization of a 13-amino acid deletion in VP1 (1D) protein and novel amino acid substitutions in 3D polymerase protein of foot and mouth disease virus subtype A/Iran87
Majid Esmaelizad*, Saber Jelokhani-Niaraki, Khadije Hashemnejad, Morteza Kamalzadeh, Mohsen Lotfi 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 9 363-371 (9 pages)
change at position 179. At this position, Ala was replaced with Val. Fig. 1A shows that amino acid changes among the VP1 genes of the field isolates consist of numerous nucleotide substitutions and deletions compared to the consensus sequence. Comparison of the predicted amino acid sequences of VP1 region in the field isolates revealed amino acid deletions in three Iranian vaccine isolates... The nucleotide sequence of the VP1 (1D) and partial 3D polymerase (3D) coding regions of the foot and mouth disease virus (FMDV) vaccine strain A/Iran87, a highly passaged isolate (∼150 passages), was determined and aligned with previously published FMDV serotype A sequences. Overall analysis of the amino acid substitutions revealed that the partial 3D coding region contained four amino acid alterations. Amino acid sequence comparison of the VP1 coding region of the field isolates revealed... -
Efficacy of nano-hydroxyapatite prepared by an aqueous solution combustion technique in healing bone defects of goat
Samit Kumar Nandi, Biswanath Kundu*, Samir Kumar Ghosh, Dipak Kumar De, Debabrata Basu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 9 183-192 (10 pages)
C). A stainless steel wire mesh was put on the reaction container to reduce particle loss through aerosol formation. Immediately after placement in the furnace, the mixed solution started to boil, followed by the evolution of a large volume of gases. The mass then frothed and swelled to yield foam, from where a flame appeared and burned with incandescence. At the initiation of ignition, the... of the bone defect was almost indistinguishable from the control group, in which the defect was more or less similar, although the newly formed bony tissue was more organized when HAp was used. Histologic methods showed complete normal ossification with development of Haversian canals and well-defined osteoblasts at the periphery in group II, whereas the control group had moderate fibro-collagenization and an adequate amount of marrow material, fat cells, and blood vessels. An oxytetracycline... -
Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea
Jae Ku Oem, Kwang Nyeon Lee, In Soo Cho, Soo Jeong Kye, Jee Yong Park, Jong Hyeon Park, Yong Joo Kim, Yi Seok Joo, Hee Jong Song 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 5 117-124 (8 pages)
cells, C; Control BHK-21 cells, D; Control IB-RS-2 cells. ..PAGE:6 122 Jae Ku Oem et al. observed between these two strains, several replacements of amino acid residues at position 54, 144, and 174 in VP1 were shown. Since the amino acid at position 144 has been known to be the major antigenic site [4], further molecular analysis between these two strains will be of great interest to elucidate... from A to T were observed in O/SKR/2002 viruses. For antigenic site 2 and 4, there were not significant variations in general. At the antigenic site 3, the substitutions of amino acid residues were present at positions 54 and 56 in O/SKR/ 2002 isolates and an alternative residue I at position 54 are observed only at the sequence of O/SKR/AS/2002 (cow) virus. And the substitution (L→P) of significant residue at position 144 was detected at the amino acid sequence of the O/SKR/2002 (cow) virus. -
Sequence Analysis of Canine LINE-1 Elements and p53 Gene in Canine Transmissible Venereal Tumor
Young-Ki Choi and Chul-Joong Kim 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 6 285-292 (8 pages)
a single point mutation at 964 nucleotide position from T → C.From the this point mutation amino acid was changed from Phe to Ser at codon 316. To confirmed the point mutation at the TVT genomic DNA, we designed the primers (ca p53 931: 5′-CGCAAAA GAAGAAGCC ACTA-3′and caREV) for the exon 9 and 10. The PCR products were purified atthe 0.7% agarose gel and cloned into TA cloning vector and then... LINEs (long interspersed nuclear elements or long interspersed repeated DNA elements) contains two open reading frames (ORFs), ORF1 and ORF2. We analysed the ORF2 located in the 5' region to the first exon of oncogene c-myc in canine transmissible venereal tumor (TVT) cell. We also showed the transcription activation was induced by this TVT-LINE sequence using CAT assay. To identify the mutation of tumor suppressor gene, sequence analysis of p53 from TVT cell was performed. We identified the...