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무문토기시대 가구(家口) 연구
Rachel Lee, 윤호필(Ho-Pil Yun), 박용근(Yong-Geun Park) 한국고고학회 한국고고학전국대회 발표문 25 Pages
한국고고학회 한국고고학전국대회 발표문 제37회 한국고고학대회 8 162-186 (25 pages)
a microscope using plane polarized light (PPL), crossed polarized light (XPL), and oblique incident light (OIL) under magnifications from X 2.5 to X 40. Descriptions followed guidelines from Bullock et al. (1985) and Stoops (2003). Scans of each thin section were inverted in grayscale in Photoshop for porosity comparisons. Six areas of interest from one thin section were analyzed by energy... -
Effect of phosphorus deficiency on erythrocytic morphology and function in cows
Ziwei Zhang, Mingyu Bi, Jie Yang, Haidong Yao, Zhonghua Liu, Shiwen Xu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 10 333-340 (8 pages)
transmission electron microscope (JEM-100CX; Nihon Densi, Japan). Assay of aminotransferase activities in serum Aminotransferase activities were analyzed by using coupled kinetic assay methods [16]. The aspartate transaminase (AST) assays used a coupled enzymatic reaction (AST kit 000000020; Biosino Bio-Technology and Science, China). Initially, aspartate and -ketoglutarate were converted to... The aim of this study was to evaluate the influence of phosphorus (P) deficiency on the morphological and functional characteristics of erythrocytes in cows. Forty Holstein-Friesian dairy cows in mid-lactation were randomly divided into two groups of 20 each and were fed either a low-P diet (0.03% P/kg dry matter [DM]) or a control diet (0.36% P/kg DM). Red blood cell (RBC) indices results showed RBC and mean corpuscular hemoglobin decreased while mean corpuscular volume increased significantly... -
Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions
Ying-Xiao Fu, Jian-Hong Gu, Yi-Ran Zhang, Xi-Shuai Tong, Hong-Yan Zhao, Yan Yuan, Xue-Zhong Liu, Jian-Chun Bian, Zong-Ping Liu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 5 405-412 (8 pages)
an inverted phase contrast fluorescence microscope (Leica Microsystems) and the green filters. Pit resorption assay Duck embryo bone marrow cells were seeded on 48-well plates with bovine cortical bone slices. After culturing, the remaining cells on the slices were lysed with 0.25 mol/L NH 4OH. Excavations in the slice surface were observed with an environmental scanning electron microscope... The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor κB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0, 10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast... -
Expression of E-cadherin in pig kidney
Su-Youn Lee, Sun M. Han, Ji-Eun Kim, Ku-Yong Chung, Ki-Hwan Han 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 2 381-386 (6 pages)
with a transmission electron microscope (H-7650; Hitachi, Japan). Double immunocytochemistry To identify the exact localization of E-cadherin, double immunoperoxidase techniques as previously described [9,10,13]. For the first immunoperoxidase procedure, the protocol for single labeling was performed as described above. After the diaminobenzidine reaction, the sections were washed in PBS,... E-cadherin is a cell adhesion molecule that plays an important role in maintaining renal epithelial polarity and integrity. The purpose of this study was to determine the exact cellular localization of E-cadherin in pig kidney. Kidney tissues from pigs were processed for light and electron microscopy immunocytochemistry, and immunoblot analysis. E-cadhedrin bands of the same size were detected by immunoblot of samples from rat and pig kidneys. In pig kidney, strong E-cadherin expression was... -
The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin
Yi-Pin Lin, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 3 133-144 (12 pages)
by EPM (Nikon, Japan) or CLSM (Olympus, Japan). An Olympus Fluoview 500 confocal laser-scanning imaging system, equipped with krypton, argon and He-Ne lasers on an Olympus IX70 inverted microscope with a PLAPO 60 × objective, was used. The settings were identical for all cap- tured images. Images were processed using Adobe Photoshop CS2. For counting the attachment of Leptospira to the... Adhesion through microbial surface components that recognize adhesive matrix molecules is an essential step in infection for most pathogenic bacteria. In this study, we report that LigB interacts with fibronectin (Fn) through its variable region. A possible role for LigB in bacterial attachment to host cells during the course of infection is supported by the following observations: (i) binding of the variable region of LigB to Madin-Darby canine kidney (MDCK) cells in a dose-dependent manner... -
Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD
Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 2 7-19 (13 pages)
O157:H7 13 Vero cells was examined under an inverted microscope (DMIRB/E; Leica, Germany). In this study, we determined that “weak” was ranging from 0% to 30%, and “strong” was from 30% to 100% of Vero cells were dead. The result was shown in Table 5. Antimicrobial susceptibility test The antimicrobial susceptibility of 11 E. coli O157:H7 isolates was determined by Bauer and Kirby method [5].... Escherichia coli O157:H7 is recognized as a significant food-borne pathogen, so rapid identification is important for food hygiene management and prompt epidemiological investigations. The limited prevalence data on Shiga toxin-producing E. coli (STEC) and E. coli O157:H7 in foods and animals in Korea made an assessment of the risks difficult, and the options for management and control unclear. The prevalence of the organisms was examined by newly developed kit-E. coli O157:H7 Rapid kit. For the... -
In vivo evaluation of preventive effect of Lactobacillus reuteri on porcine epidemic diarrhea in suckling piglets
YuRiOh, JoongBokLee, SeungYongPark, ChangSeonSong, InSooChoi, YongHyunKim, EunJungHan, JungHeeLee, KwangSeiLim, ChulSungHuh, Seo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2008, 제 48권 제 2호 6 167-174 (8 pages)
solutions. The tissues were stained with Gill’s hematoxylin (Merck, USA) and eosin (Merck, USA) and cleared in xylene (Merck, USA). Histopathologic changes were analyzed using an Eclipse TE2000-U Microscope (Nikon, Japan). Statistical analysis Statistical analysis of survival rate of each groups were performed by Fisher’s Exact test. The SAS 9.1 software package (SAS Institute, USA) was used for... -
Golgi and Cajal’s scientific inquiry processes on the Neuron
Won Jung Kim, Yong-Ju Kwon 한국교원대학교 뇌기반교육연구소 Brain & Learning 16 Pages
한국교원대학교 뇌기반교육연구소 Brain & Learning 2012, 제 2권 제 1호 2 12-27 (16 pages)
cerebellum were stained and observed by the light microscope. Collecting result: Brain fragments including cerebellum, retina, and the spinal cord were stained and observed by the light microscope. Evaluating hypothesis and Stating conclusion : “All samples showed network and the reticular theory explains the structure of the nerve system” Evaluating hypothesis and Stating conclusion :... 생물학사를 적용한 생물 탐구는 과학자들의 연구 과정을 고찰하고 학생들이 과학자의 탐구 과정을 이해할 수 있는 효과적인 방법이다. 본 연구는 1906년 노벨 생리의학상 공동 수상자인 골지와 카잘의 연구 과정을 되짚어본다. 이 연구의 목적은 먼저 골지와 카잘의 연구 과정을 살펴보고, 이를 바탕으로 뉴런과 관련한 내용을 학습할 때 활용할 수 있는 생물학사 기반 탐구 활동지를 개발하는 것이다. 이를 위해 먼저 과학 탐구 과정에 대해 소개하였다. 이 과정에 각각의 과학자가 수행한 탐구 과정을 대응시키는 과학자의 탐구 과정... -
Effects of Lubiprostone on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Colon
Han-YiJiao, DongHyunKim, JungSukKi, KwonHoRyu, SeokChoi, JaeYeoulJun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.4 9 341-346 (6 pages)
under a confocal laser-scanning microscope (FV300; Olympus, Japan) at an excitation wave- length appropriate for FITC (488 nm). Patch-clamp experiments The whole-cell configuratio n of the patch-clamp techni- que was used to record pacemaker potentials in colonic ICCs that showed the network- like structures in culture (2 ∼3 days). Pacemaker potentia ls were amplified using Axopatch 200B (Axon... Lubiprostone is a chloride (Cl−) channel activator derived from prostaglandin E1 and used for managing constipation. In addition, lubiprostone affects the activity of gastrointestinal smooth muscles. Interstitial cells of Cajal (ICCs) are pacemaker cells that generate slow-wave activity in smooth muscles. We studied the effects of lubiprostone on the pacemaker potentials of colonic ICCs. We used the whole-cell patch-clamp technique to determine the pacemaker activity in cultured colonic... -
The Effect of LidocaineㆍHCl on the Fluidity of Native and Model Membrane Lipid Bilayers
Jun-SeopPark, Tae-SangJung, Yang-HoNoh, Woo-SungKim, Won-IckPark, Young-SooKim, In-KyoChung, UyDongSohn, Soo-KyungBae, Moon-KyoungBae, Hye 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.6 7 413-422 (10 pages)
special temperature-controlled chamber, which was previously described [26,27], was used. The experi- ments were carried out in the same chamber after the vesi- cle formation, using an invert ed microscope (Axiovert35: Zeiss, Thornwood, NY). The following steps were used to prepare the GUVs: 1) ∼3 μl of the lipid and phospholipid stock solution was spread on each Pt wire under a stream on N... The purpose of this study is to investigated the mechanism of pharmacological action of local anesthetic and provide the basic information about the development of new effective local anesthetics. Fluorescent probe techniques were used to evaluate the effect of lidocaineㆍHCl on the physical properties (transbilayer asymmetric lateral and rotational mobility, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex, and...