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Improvement of pregnancy rate by intrauterine administration of dexamethasone and recombinant human leukemia inhibitory factor at the time of embryo transfer in cattle
Sangho Roh, Se-Woong Kim, Yeon-Gil Jung, Jong-Im Park 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 17 569-576 (8 pages)
enhanced pregnancy rate. Keywords: bovine, embryo transfer, fertilization in vitro, pregnancy rate, prostaglandins F Introduction Embryo transfer (ET) techniques, such as nonsurgical transfer/recovery and cryopreservation of embryos, superovulation, preparation of recipient animals, and in vitro production (IVP) of embryos, have developed into well-organized processes supporting many branches... 0.05). Embryo transfer (ET) was performed with blastocysts (day 7). PGF2α levels of control recipient cows were significantly higher in the circulatory blood samples collected 60 min after ET than in samples collected 60 min before ET (p ＜ 0.005), and were decreased in cows injected with loading medium supplemented with Dex+rhLIF (p ＜ 0.005). Pregnancy rate was significantly higher in the ET group that received supplemented embryo-loading medium than in the non-supplemented control... -
Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus)
Sang Tae Shin, *, Sung Keun Jang, Hong Suk Yang, Ok Keun Lee, Yhong Hee Shim, Won Il Choi, Doo Soo Lee, Gwan Sun Lee, Jong Ki Cho 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 14 103-107 (5 pages)
by ovum pick-up and embryo recovery but not for embryo transfer. Estrus synchronization is essential for successful ET and corpus luteum (CL) formation is necessary for pregnancy maintenance. However, it is difficult to know the exact status of the ovaries if they are not observed directly by exploratory surgery or ultrasonography. Therefore, if the CL is not formed during ET, artificial... This study was performed to produce transgenic Korean native goat (Capra hircus) by laparoscopic embryo transfer (ET) to overcome the limitations of ET performed by laparotomy. Transgenic embryos were produced by DNA pronuclear microinjection of in vivo zygotes. The recipient goats were synchronized for estrus by using an introvaginal progesterone devices as a controlled internal drugreleasing insert (CIDR) for 13 days and injection of 400 IU PMSG 48 h before removal of the insert. Embryos were... -
Prevalence and characterization of infectious disease in wild birds from the South Korea
Se Yeoun Cha, Min Kang, Bai Wei, Woo seog Jeong, Yong Myung Kang, Hong Sik Park, Hyung Kwan Jang 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 215 310-311 (2 pages)
Western blot analysis showed the Antigenicities of recombinant Apx partial antigens. However cross-reactivity was also observed in Western blot analysis. When compared to a negative serum of rabbit, the sera of rabbits primed by full antigen of each Apx toxin showed specific- responses to each of recombinant Apx partial antigen. The diagnostic ELISA methods are being optimized using the... -
Development of Actinobacillus pleuropneumoniae ApxІ, II, III, and IV-specific Diagnostic ELISA Methods based on the Recombinant Apx Antigens
Myung hwan Jung, Ho Keun Won, Myung Whan Oh, Min Kyoung Shin, Han Sang Yoo 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 214 309-310 (2 pages)
Western blot analysis showed the Antigenicities of recombinant Apx partial antigens. However cross-reactivity was also observed in Western blot analysis. When compared to a negative serum of rabbit, the sera of rabbits primed by full antigen of each Apx toxin showed specific- responses to each of recombinant Apx partial antigen. The diagnostic ELISA methods are being optimized using the... -
Rotavirus induces necroptosis through RIP1/RIP3/MLKL signaling pathway
Ja Young Seo, Sang Ik Park, Mun Il Kang, Kyoung Oh Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 57 209-210 (2 pages)
by treatment of NA and SNL as well as by incubate d with NANA, NGNA and α indicating usage of α2,6-linked terminal SA as an attachment factor for these strains. Both strains were agglutinated rabbit erythrocytes which expressing α did not agglutinate erythroc ytes from other species including cows and pigs. Fu rthermore. hemagglutination to rabbit erythrocyte was decreased by treatment of... -
In vitro treatment of lipopolysaccharide increases invasion of Pasteurella multocida serotype B:2 into bovine aortic endothelial cells
Seng Kar Yap, Zunita Zakaria, Siti Sarah Othman, Abdul Rahman Omar 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 7 207-215 (9 pages)
P. multocida B:2 strain and the anti-P. multocida B:2 polyclonal rabbit serum, respectively. Conflict of Interest The authors declare no conflicts of interest. References 1. Al-haj Ali H, Sawada T, Hatakeyama H, Katayama Y, Ohtsuki N, Itoh O. Invasion of chicken embryo fibroblast cells by avian Pasteurella multocida. Vet Microbiol 2004, 104, 55-62. 2. Anderson BH, Watson DL, Colditz IG. The... Pasteurella multocida serotype B:2 causes hemorrhagic septicemia in cattle and buffalo. The invasion mechanism of the bacterium when invading the bloodstream is unclear. This study aimed to characterize the effects of immunomodulatory molecules, namely dexamethasone and lipopolysaccharide, on the invasion efficiency of P. multocida serotype B:2 toward bovine aortic endothelial cells (BAECs) and the involvement of actin microfilaments in the invasion mechanism. The results imply that treatment of... -
Tibial dyschondroplasia is closely related to suppression of expression of hypoxia-inducible factors 1α, 2α, and 3α in chickens
Shucheng Huang, Mujeeb U. Rehman, Gang Qiu, Houqiang Luo, Muhammad K. Iqbal, Hui Zhang, Khalid Mehmood, Jiakui Li 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 14 107-115 (9 pages)
at 4 oC with rabbit monoclonal anti-HIF-1α antibodies (1:1,000 dilution, A11945; ABclonal Technology, China). The membranes were washed 3 times with PBS Tween 20 for 5 min each, then incubated with a secondary antibody (1:3,000 dilution; horseradish peroxidase labeled rabbit anti-goat secondary antibodies) for 1 h at room temperature. After washing, the bands were visualized and exposed by... Tibial dyschondroplasia (TD) cases has not been reported in Tibetan chickens (TBCs), but it is commonly seen in commercial broilers characterized by lameness. The underlying mechanism remains unclear. Hypoxia-inducible factors (HIFs) are important regulators of cellular adaptation to hypoxic conditions. In this study, we investigated the role of HIF-1α, -2α, and -3α in hypoxia and thiram-induced TD and their effect on tibial growth plate development in Arbor Acres chickens (AACs) and TBCs.... -
Coactosin-like protein 1 inhibits neuronal migration during mouse corticogenesis
Guohong Li, Yupeng Yin, Jiong Chen, Yanle Fan, Juhong Ma, Yingxue Huang, Chen Chen, Pengxiu Dai, Shulin Chen, Shanting Zhao 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 4 21-26 (6 pages)
and agents Antibodies included rabbit anti-green fluorescent protein (GFP), goat anti-myc, 4’,6-diamidino-2-phenylindole (DAPI; Millipore, USA), Alexa Fluor 488-conjugated donkey anti- rabbit IgG and Alexa Fluor 568-conjugated donkey anti-goat IgG (Life Technologies, USA). Bovine serum albumin, Triton X-100, and normal goat serum were purchased from Sigma-Aldrich (USA). RNA bioanalyzer A... Coactosin-like protein 1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from a soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains undescribed. In this study, we revealed that Cotl1 overexpression impaired migrationof both early- and late-born neurons during mouse corticogenesis.... -
Chicken RNA-binding protein T-cell internal antigen-1 contributes to stress granule formation in chicken cells and tissues
Yingjie Sun, Pin Zhang, Hang Zheng, Luna Dong, Lei Tan, Cuiping Song, Xusheng Qiu, Ying Liao, Chunchun Meng, Shengqing Yu, Chan 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 2 3-12 (10 pages)
(A11055), Alexa Fluor 594-labeled goat anti-rabbit (A11012), and goat anti-mouse (A-11005) were purchased from Thermo Fisher Scientific (USA). Cells and viruses HeLa and DF-1 chicken fibroblast cells were purchased from American Type Culture Collection (ATCC; USA). Chicken embryo fibroblast (CEF) primary cells were prepared from 9-day-old specific-pathogen-free (SPF) embryonated chicken eggs... T-cell internal antigen-1 (TIA-1) has roles in regulating alternative pre-mRNA splicing, mRNA translation, and stress granule (SG) formation in human cells. As an evolutionarily conserved response to environmental stress, SGs have been reported in various species. However, SG formation in chicken cells and the role of chicken TIA-1 (cTIA-1) in SG assembly has not been elucidated. In the present study, we cloned cTIA-1 and showed that it facilitates the assembly of canonical SGs in both human and...