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전체 선택해제
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본문검색 결과만 보기
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DevelDevelopment of a Quantitative Real-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid
Seong Heo, Hyun Ran Kim, Hee Jae Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 8 164-171 (8 pages)
at a low isothermal tem- perature, generally at 41 oC. This process exponentially am- plifies the products producing single-stranded RNA of op- posite sense to the original target as the major amplification product (Hibbitts et al., 2003). The amplification products have been detected through a probe-capture hybridization using electrochemiluminescence in molecular beacons. We have developed... of the starting ASSVd RNA copy number of 10-fold dilutions each. Compared to the established RT-PCR methods, our method was more sensitive for detecting ASSVd. The real-time quantitative NASBA method will be fast, sensitive, and reliable for routine diagnosis and selection of viroid-free stock materials. Furthermore, real-time quantitative NASBA may be especially useful for detecting low levels in apple trees with early viroid-infection stage and for monitoring the influence on tree growth. -
Implementation Of 802.15.4 Wireless Sensor Network in Real-time Monitoring and Control System for GreenHouse
Jaypal Baviskar, Afshan Mulla, , Amol Baviskar, Jeet Desai 인문사회과학기술융합학회 예술인문사회융합멀티미디어논문지 19 Pages
인문사회과학기술융합학회 예술인문사회융합멀티미디어논문지 2015, 제 5권 제 5호 10 87-105 (19 pages)
in Real-timeMonitoring and Control Systemfor GreenHouse Jaypal Baviskar1),AfshanMulla 2),Amol Baviskar3),JeetDesai4) Abstract In order to meet the increasing need for quality conscience crops and to utilize the resources efficiently, the concept of Greenhouse was introduced. It assists in accurate monitoring and controlling of various parameters, in order to nurture healthy crops. In this paper... controlling of various parameters, in order to nurture healthy crops. In this paper efforts are made to acknowledge the problems associated with maintaining precise environmental conditions, and to implement a sophisticated system to supervise the real-time monitoring and controlling operation inside the Greenhouse. The necessity for an Automated System deploying wireless communication and remote sensing is considered. This paper illustrates the implementation of a WSN (Wireless Sensor Network)... -
Real-Time, Simultaneous and Proportional Myoelectric Control for Robotic Rehabilitation Therapy of Stroke Survivors
YoungJin Jung, Hae Yean Park, Kinsuk Maitra, Nagarajan Prabakar, Jong-Hoon Kim 대한신경계작업치료학회 재활치료과학 10 Pages
대한신경계작업치료학회 재활치료과학 2018, 제 7권 1호 6 79-88 (10 pages)
No. 1. 2018. https://doi.org/10.22683/tsnr.2018.7.1.079 Real-Time, Simultaneous and Proportional Myoelectric Control for Robotic Rehabilitation Therapy of Stroke Survivors YoungJin Jung*, Ph.D., Hae Yean Park**, Ph.D., O.T., Kinsuk Maitra***, PhD, O.T.R./L., F.A.O.T.A., Nagarajan Prabakar****, Ph.D., Jong-Hoon Kim*****, Ph.D. *Dept. of Radiological Science at Health Science Division, Dongseo... novel technical healthcare solution for the real-time, simultaneous and propositional myoelectric control for stroke survivors’ upper limb robotic rehabilitation therapy. Methods : To implement an appropriate computational algorithm for controlling a portable rehabilitative robot, a linear regression model was employed, and a simple game experiment was conducted to identify its potential of clinical utilization. Results : The results suggest that the proposed device and computational algorithm... -
실시간 TEM 분석에 유용한 영상 기록 프로그램, VirtualDub
김진규, 오상호, 송경, 유승조, 김영민, Kim. Jin-Gyu, Oh. Sang-Ho, Song. Kyung, Yoo. Seung-Jo, Kim. Young-Min 한국현미경학회 한국현미경학회지 5 Pages
한국현미경학회 한국현미경학회지 2010, Vol.40 No.1 47-51 (5 pages)
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Extendedspectrum beta-lactamase genes among multidrug resistant Escherichia coli isolates from horses in Korea
Hong Bum Koh, Joon Young Kim, Dae Hyun Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 132 400-401 (2 pages)
by real-time PCR using PBMC. Results: The results of serological study showed that 41 samples out of 371 are positive. In real-time PCR assay, 41 samples are positive. Conclusions: Positive percentage was 11% in both serological and molecular diagnostic tests. P-049 Genotypic and Phenotypic Characterization of Shiga Toxin-producing Escherichia coli Isolates from the Retail Meats and Meat... -
Activation of autophagy at cerebral cortex and apoptosis at brainstem are differential responses to 835 MHz RF-EMF exposure
JuHwanKim, Da-HyeonYu, HakRimKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 5 179-188 (10 pages)
in constant temperature. Quantitative real-time PCR and semi-quantitative RT- PCR Total RNA was purified from the whole cerebral cortex of the both group of mice by using TRIzol reagent (Thermo Fisher Scientific, USA). RNA was reverse transcribed to cDNA using MMLV Reverse-Transcriptase (Bioneer, South Korea) and an oligo-d(T)18 primer. Quantitative RT-PCR reactions were performed with... With the explosive increase in exposure to radiofrequency electromagnetic fields (RF-EMF) emitted by mobile phones, public concerns have grown over the last few decades with regard to the potential effects of EMF exposure on the nervous system in the brain. Many researchers have suggested that RF-EMFs can effect diverse neuronal alterations in the brain, thereby affecting neuronal functions as well as behavior. Previously, we showed that long-term exposure to 835 MHz RF-EMF induces autophagy in... -
Improvement Characteristics of Bio-active Materials Coated Fabric on Rat Muscular Mitochondria
DongheeLee, Young-WonKim, Jung-HaKim, MisukYang, HyemiBae, InjaLim, HyoweonBang, Kyung-ChanGo, Gwang-WungYang, Yong-HwanRho, Hyo-SukPark, E 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.3 14 283-289 (7 pages)
1 alpha; β-actin, beta-actin; 18S, 18S ribo- somal RNA; CASP3, caspase 3; CASP9, caspase 9; FasR, Fas receptor. *Q, mean primer for real-time PCR. Fig. 1. Effect of BMCF on the expression of oxygen consumption. V-Con, 0% BMCF group; V-10, 10% BMCF group; V-30, 30% BMCF group. **p＜0.01, ***p＜0.001. are shown in Table 1. Each real-time PCR reaction mixture included 0.4 μl MgCl 2 (final... This study surveys the improvement characteristics in old-aged muscular mitochondria by bio-active materials coated fabric (BMCF). To observe the effects, the fabric (10 and 30%) was worn to old-aged rat then the oxygen consumption efficiency and copy numbers of mitochondria, and mRNA expression of apoptosis- and mitophagy-related genes were verified. By wearing the BMCF, the oxidative respiration significantly increased when using the 30% materials coated fabric. The mitochondrial DNA copy... -
Berberine Prevents Intestinal Mucosal Barrier Damage During Early Phase of Sepsis in Rat through the Toll-Like Receptors Signaling Pathway
Guo-xunLi, Xi-moWang, TaoJiang, Jian-fengGong, Ling-yingNiu, NingLi 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.1 1 1-8 (8 pages)
in rat through mechanisms independent of the NOD-like receptors signaling pathway. Eur J Pharmacol. 2014; 730:1-7. 25. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25:402-408. 26. Takeuchi O, Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa T, Takeda K, Akira S. Differential roles of TLR2 and... Our previous study has shown berberine prevents damage to the intestinal mucosal barrier during early phase of sepsis in rat through mechanisms independent of the NOD-like receptors signaling pathway. In this study, we explored the regulatory effects of berberine on Toll-like receptors during the intestinal mucosal damaging process in rats. Male Sprague-Dawlay (SD) rats were treated with berberine for 5 d before undergoing cecal ligation and puncture (CLP) to induce polymicrobial sepsis. The... -
Bcl-2 Knockdown Accelerates T Cell Receptor-Triggered Activation-Induced Cell Death in Jurkat T Cells
Yun-JungLee, TaeJoonWon, KyeongEunHyung, MiJiLee, Young-hyeMoon, IkHeeLee, ByungSungGo, KwangWooHwang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.1 10 73-78 (6 pages)
a tem- plate, real-time polymerase chain reaction (PCR) was per- formed employing SYBR Green Master Mix (Applied Bio- systems) on a LightCycler system (Roche). The relative ex- pression levels, normalized wi th respect to the expression of GAPDH, were then calculated as fold induction compared to the scrambled control without any stimulation. Western blot analysis Bcl-2-knockdown cell lines were... Cell death and survival are tightly controlled through the highly coordinated activation/inhibition of diverse signal transduction pathways to insure normal development and physiology. Imbalance between cell death and survival often leads to autoimmune diseases and cancer. Death receptors sense extracellular signals to induce caspase-mediated apoptosis. Acting upstream of CED-3 family proteases, such as caspase-3, Bcl-2 prevents apoptosis. Using short hairpin RNAs (shRNAs), we suppressed Bcl-2... -
High Glucose Induces Connective Tissue Growth Factor Expression and Extracellular Matrix Accumulation in Rat Aorta Vascular Smooth Muscle Cells Via Extracellular Signal-Regulated Kinase 1/2
YuMiHa, DongHyupLee, MinaKim, YoungJinKang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.4 8 307-314 (8 pages)
hr at room temperature. Finally, after rinses with the wash buffer, the membranes were exposed to ECL western blot analysis detection reagents. Real-time quantitative RT-PCR analysis The total RNA was isolated from the VSMCs using the RNeasy Mini Kit (Qiagen Inc. , Valencia, CA, USA) accord- ing to the manufacturer’s instructions. RT-PCR was per- formed using 1 μg of total RNA. The total RNA... Connective tissue growth factor (CTGF) is a potent pro-fibrotic factor, which is implicated in fibrosis through extracellular matrix (ECM) induction in diabetic cardiovascular complications. It is an important downstream mediator in the fibrotic action of transforming growth factor Ղ (TGFՂ) and is potentially induced by hyperglycemia in human vascular smooth muscle cells (VSMCs). Therefore, the goal of this study is to identify the signaling pathways of CTGF effects on ECM...