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Development and Validation of a Liquid Chromatography Method with Electrospray Ionization Tandem Mass Spectrometry for the Determination Acrinol, Loperamide, and Berberine Residues in Pork
Dana Jeong, Jin A Park, Dan Zhang, Seong Kwan Kim, Sang Hyun Cho, Ho Chul Shin 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 151 217-217 (1 pages)
to dryness and diluted with mobile phase and the solution was used for analysis. Results: The limit of quantification for acrinol was 1 μ for loperamide was 0.25 μg/kg, and for berberine was0.5 μg/kg. The mean recoveries of nitrovin ranged from 82.39 ± 4.01 to 86.1 ± 6.4 in matrices (Mean ± RSD, %). The method was selective, and good accuracy, precision, recovery, and stability were... -
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca2+ Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat
JeongHoonLee, JeongMiHa, ChaeHunLeem 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.4 10 373-382 (10 pages)
we could calculate Kd. To calculate the free Ca 2+ concentration, WINMAXC32 version 2.50 (Chris Patton, Stanford University) was used. EGTA purity was double-check ed using the pH and oxalate method [38-42]. We obtained pH-dependent changes of K d values in a cell-free solution. To simulate the experimental conditions, we measured pH-dependent K d values with a dye-free cell in the window.... a novel online method that corrected NADH and Fura-2-FF interference. We simultaneously measured multiple parameters, including NADH, [Ca2+], and pH/mitochondrial membrane potential; Fura-2-FF for mitochondrial [Ca2+] and TMRE for Ψm or carboxy-SNARF-1 for pH were used. With this novel method, we found that the resting mitochondrial [Ca2+] concentration was 1.03 μM. This 1 μM cytosolic Ca2+ could theoretically increase to more than 100 mM in mitochondria. However, the mitochondrial [Ca2+]... -
Ameliorative Effect of a Selective Endothelin ETA Receptor Antagonist in Rat Model of L-Methionine-induced Vascular Dementia
GautamjeetSMangat, AmteshwarSJaggi, NirmalSingh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.3 2 201-209 (9 pages)
a Selective Endothelin ET A Receptor Antagonist in Rat Model of L-Methionine-induced Vascular Dementia Gautamjeet S Mangat, Amteshwar S Jaggi, and Nirmal Singh Pharmacology Division, Department of Pharmaceutical Sciences and Drug Research, Faculty of Medicine, Punjabi University, Patial a (Punjab) 147002, India The present study was designed to investigate the efficacy of selective ET A receptor... The present study was designed to investigate the efficacy of selective ETA receptor antagonist, ambrisentan on hyperhomocysteinemia-induced experimental vascular dementia. L-methionine was administered for 8 weeks to induce hyperhomocysteinemia and associated vascular dementia in male rats. Ambrisentan was administered to L-methionine-treated effect rats for 4 weeks (starting from 5th to 8th week of L-methionine treatment). On 52nd day onward, the animals were exposed to the Morris water maze... -
Mechanisms of Selective Antimicrobial Activity of Gaegurin 4
HeejeongKim, ByeongJaeLee, MunHanLee, SeongGeunHong, PanDongRyu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.1 7 39-48 (10 pages)
＋ ion-selective electorde (Phoenix Co.) connected to an ion meter at 22∼ 24 o C. The electrode exhibits a Nernstian response (53 mV/decade) from 10 5 to 10 3 M. The cell viability was also determined during K ＋ efflux experiment. Briefly, bacterial samples were taken at fixed in tervals, diluted with the in- cubation solution and disper sed on a Luria Bertani agar medium. The viability of... GGN4 were not selective in the bilayers formed with lipids from E. coli and red blood cells. Addition of an acidic phospholipid, phosphatidylserine to planar bilayers increased the GGN4-induced membrane conductance (p＜0.05), but addition of phosphatidylcholine or cholesterol reduced it (p＜0.05). Transmission electron microscopy revealed that GGN4 induced pore-like damages in M. luteus and dis-layering damages on the outer wall of E. coli. Taken together, the present results indicate that the... -
A Simple Method for Predicting Hippocampal Neurodegeneration in a Mouse Model of Transient Global Forebrain Ischemia
Kyung-OkCho, Seul-KiKim, Young-JinCho, Ki-WugSung, SeongYunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2006, Vol.10 No.4 1 167-172 (6 pages)
by a selective estrogen receptor beta agonist in a mouse model of global ischemia. Am J Physiol Heart Circ Physiol 287: H1501-H1504, 2004 Chan PH. Mitochondria and neuronal death/survival signaling pathways in cerebral ischemia. Neurochem Res 29: 1943-1949, 2004 Fujii M, Hara H, Meng W, Vons attel JP, Huang Z, Moskowitz MA. Strain-related differences in susceptibility to transient forebrain... and subjected to bilateral occlusion of the common carotid artery (BCCAO) for 30 min. The regional cerebral blood flow (rCBF) was measured by laser Doppler flowmetry. The plasticity of PcomA was visualized by intravascular perfusion of India ink solution. When animals had the residual cortical microperfusion less than 15% as well as the smaller PcomA whose diameter was less than one third compared with that of basilar artery, neuronal damage in the hippocampal subfields including CA1, CA2, and... -
Effect of Amrinone, a Selective Inhibitor of Phosphodiesterase III, on PMNs-induced
ByungKwonOh, HyoungKiKim, SooRanChoi, JinHoSong, EonSubPark, Byung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.1 7 43-50 (8 pages)
Krebs buffer and counted using a microscope. These neutrophil preparations were ＞95% pure, and ＞95% viable by 0.3% trypan blue as the criterion for viability. Furthermore, PMNs obtained by this method have been found to respond normally in ce ll adhesion tests (Lefer et al, 1997). Preparation of rat plasma Whole blood was obtained by performing an intracardiac puncture in anesthetized rat... Ischemia followed by reperfusion in the presence of polymorphonuclear leukocytes (PMNs) results in a marked cardiac contractile dysfunction. Amrinone, a specific inhibitor of phosphodiesterase 3, has an antioxidant activity against PMNs. Therefore, we hypothesized that amrinone could attenuate PMNs-induced cardiac dysfunction by suppression of reactive oxygen species (ROS) produced fby PMNs. In the present study, we examined the effects of amrinone on isolated ischemic (20 min) and reperfused... -
Selective Cytotoxicity of Novel Platinum(II) Coordination Complexes on Human Bladder Cancer Cell-Lines and Normal Kidney Cells
Jung-TaeKim, Young-SooRho, Jee-ChangJung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.2 10 111-117 (7 pages)
Connors et Selective Cytotoxicity of Nove l Platinum(II) Coordination Complexes on Human Bladder Ca ncer Cell-Lines and Normal Kidney Cells Jung-Tae Kim 1, Young-Soo Rho 1, and Jee-Chang Jung 2 1Department of Pharmacochemistry, College of Pharmacy, 2Department of Pharmacology, School of Medicine, Kyung Hee University, Seoul 130-701, Korea Cisplatin is often effective in cancer treatmen t, but... this study, new platinum(II) complex compounds were evaluated for selective cytotoxicity on cancer cell-lines and normal kidney cells. The new platinum complexes have demonstrated high efficacy in the cytotoxicity against human bladder carcinoma cell-lines (T-24/HT-1376). The cytotoxicity of these compounds against rabbit proximal renal tubular cells and human renal cortical tissues, was determined by MTT assay, the [3H]-thymidine uptake and glucose consumption test, and found to be quite less... -
Selective Cytotoxicity of a Novel Platinum(II) Coordination Complex on Human Bladder Cancer Cell Lines and Normal Kidney Cells
Jee-ChangJung, Joo-HoChung, Sung-GooChang, Young-SooRho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.2 11 159-167 (9 pages)
by a modification of the method described by Chung et al (1982) (Jung et al， 1992; Taub， 1997). To summarize， each kidney was perfused via the renal artery， first with PBS to remove the blood， and then with a 0.5% (wjv) solution of iron oxide. The renal cortex was removed by slicing and the renal cortical slices were homogenized in a sterile Dounce homo genizer. The tubules in the... We have synthesized a novel platinum(II) coordination complex containing cis-1,2-diaminocyclohexane (DACH) as a carrier ligand and 1,2-dichloroethane (DCE) as a leaving group. In addition, nitrate was added to improve the water-solubility. A new series of [Pt(cis-DACH)(DCE)]·2NO3(PC) was evaluated for its cytotoxic activity on T-24 and J-82 human bladder carcinoma cells and normal primary cultured kidney cells. PC has demonstrated high levels of cytotoxicity against T-24 and J-82 cells. The... -
Selective Cytotoxicity of a Novel Platinum (II) Coordination Complex on Human Gastric Cancer Cell Lines and Normal Kidney Cells
Jee-ChangJung, Young-KyuKim, Sung-VinYim, Seung-JoonPark, Joo-HoChung, Sung-GooChang, Kyung-TaeLee, Young-SooRho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.3 6 283-291 (9 pages)
Indi vidual kidneys were obtained from male New Zealaud White rabbits (2 2.5 kg). Primary cultures were initiated from purified rabbit kidney proximal tubules by a modification of the method of Chung et al (1982) (Jung et al， 1992; Taub， 1997). To summarize， each kidney was perfused via the renal artery， first with PBS， to remove the blood， aud then with a 0.5% (wjv) solution 01... We have synthesized novel platinum (II) coordination complex containing cis-1,2-diaminocyclohexane (DACH) as a carrier ligand and 1,2-bis(diphenylphosphino)ethane (DPPE) as leaving group. Furthermore, nitrate was added to improve the water-solubility. A new series of [Pt(cis-DACH)(DPPE)]·2NO3 (PC) was evaluated its antitumor activity on various MKN-45 human gastric adenocarcinoma cell-lines and normal primary cultured kidney cells. The new platinum complex demonstrated high efficacy in the... -
Synthesize multi-walled carbon nanotubes via catalytic chemical vapour deposition method on Fe-Ni bimetallic catalyst supported on kaolin
Aliyu A, Abdulkareem AS, Kovo AS, Abubakre OK, Tijani JO, Kariim I 한국탄소학회 Carbon Letters 18 Pages
한국탄소학회 Carbon Letters 2017, Vol.21 5 33-50 (18 pages)
a non- porous material, prevented the formation of amorphous carbon and hence stimulated selective CNT production. Thus, the cata- lyst support used in the substrate CVD process has an important function in infuencing the catalyst activity. In addition, different transition metals possess diverse catalytic activities and differ- ent CNT qualities. These transition metals, particularly Fe, Co,... a wet impregnation method. The effects of mass of kaolin support, pre-calcination time, pre-calcination temperature and stirring speed on catalyst yields are examined. Then, the optimal supported Fe-Ni catalyst is utilised to produce multi-walled carbon nanotubes (MWCNTs) using catalytic chemical vapour deposition (CCVD) method. The catalysts and MWCNTs prepared using the optimal conditions are characterized using high resolution transmission electron microscope (HRTEM), high-resolution scanning...