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Genetic diversity of Korean Bacillus anthracis isolates from soil evaluated with a single nucleotide repeat analysis
Sang Hoon Kim, Kyoung Hwa Jung, Se Kye Kim, Seong-Joo Kim, Ji-Cheon Kim, Soo Young Cho, Jin Choul Chai, Young Seek Lee, Yun Ki K 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 12 457-465 (9 pages)
AB, Wong ACL, Keim P, Jackson PJ. Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Appl Environ Microbiol 2004, 70, 1068-1080. 6. Jung KH, Kim SH, Kim SK, Cho SY, Chai JC, Lee YS, Kim JC, Kim SJ, Oh HB, Chai YG. Genetic population of Bacillus anthracis isolates from Korea. J Vet Sci 2012, 13, 385-393. 7.... agent of anthrax, is one of the most genetically monomorphic bacteria species in the world. Due to the very limited genetic diversity of this species, classification of isolates of this bacterium requires methods with high discriminatory power. Single nucleotide repeat (SNR) analysis is a type of variable-number tandem repeat assay that evaluates regions with very high mutation rates. To subtype a collection of 21 isolates that were obtained during a B.anthracis outbreak in Korea, we analyzed... -
Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers
Juan C. Ochoa, Mariana Herrera, M?nica Navia, Hern?n Mauricio Romero 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 3 19-31 (13 pages)
be a mixture of transformed and untransformed mycelia (Table 2). Using Southern blotting, we found that the single zoo- spore cultures had the same number of insertions as the parental cultures, indicating that the T-DNA had been properly integrated into the genome. However, qRT-PCR analysis revealed small variations in the expression levels of fluorescent protein, indicating that colonies... to include the fluorescent proteins CFPSKL (peroxisomal localization), eGFP and mRFP1 (cytoplasmic localization). The stability of some transformants was confirmed by Southern blot analysis and single zoospore cultures; additionally, virulence and in vitro growth were compared to the wild-type isolate to select transformants with the greatest resemblance to the WT isolate. GFP-tagged P. palmivora was useful to identify all of the infective structures that are commonly formed by hemibiotrophic... -
Methylome Analysis of Two Xanthomonas spp. Using Single-Molecule Real-Time Sequencing
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 2 500-507 (8 pages)
protocol (Pacific Biosciences). Briefly, fluorescent signals were quantitated kinetically as part of the SMRT sequencing process. DNA modifcations altered the kinetic character- istics of time for base incorporation and measured chang- es in interpulse duration (IPD). The IPD ratio was calcu- lated by comparing the observed IPD to the in silico IPD control, and a base modifcation quality value... Single-molecule real-time (SMRT) sequencing allows identification of methylated DNA bases and methylation patterns/motifs at the genome level. Using SMRT sequencing, diverse bacterial methylomes including those of Helicobacter pylori, Lactobacillus spp., and Escherichia coli have been determined, and previously unreported DNA methylation motifs have been identified. However, the methylomes of Xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been... -
Safety and Immunogenicity of Recombinant rabies virus (ERAGS) in raccoon dogs
Dong Kun Yang, Ha Hyun Kim, Hyun Ye Jo, Sung Suk Choi, In Cheol Choi, Kang Bok Lee, In soo Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 253 473-474 (2 pages)
recombinant rabies virus named as ERAGS strain was recovered in BHK/T7-9 cells. After second passage of constructed recombinant rabies virus, the titration of the ERAGS was also done by indirect fluorescent assay (IFA) and the viral titers were determined by 50% fluorescent assay infectious dose per ml (FAID 50/mL). In addition, the authenticity of G gene of the ERAGS stain was confirmed by... -
Safety and Immunogenicity of live rabies vaccine strain (ERAGS) in animals
Dong Kun Yang, Ha Hyun Kim, Sung Suk Choi, Tae Hyun Ahn, Ho Ryoung Kim, In Soo Cho, Woong Ho Jeong 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 185 291-292 (2 pages)
a fixed rabies strain, was used for fluorescent antibody virus neutralization (FAVN) tests. Safety and immunogenicity of ERAGS strain in animals : Guinea pigs were divided into seven groups consisting of four heads and each group was inoculated with ERAGS (10 4.0 TCID50/mL to 10 2.0 TCID50/mL) via intramuscular (IM) or subcutaneous (SC) injections. Three-month-old dogs, seronegative against... -
In Vitro Screening of Probiotic Potentials of Bacteria Isolated From Korean Fermented Foods and Wild Birds
Sol Jeong, Gyeong Bin Gwon, Kyu Jik Kim, Woo Taek Hong, Yu Jin Kim, Jung Hoon Kwon, Chang Seon Song 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 133 262-263 (2 pages)
fluorescent antibody virus neutralisation test (FAVNtest) for the quantitation of rabies-neutralising antibody. J Immunol Methods 212: 79-87. P-073 In Vitro Screening of Probiotic Potentials of Bacteria Isolated From Korean Fermented Foods and Wild Birds Sol Jeong 1, Gyeong-Bin Gwon 1, Kyu-Jik Kim 1, Woo-Taek Hong 1, Yu-Jin Kim 1, Jung-Hoon Kwon 1, Chang-Seon Song *1 1Avian Disease Laboratory,... -
NIH Potency Test for Rv-Fc and Rv-K BasedCandidate Anti-Rabies Vaccine Produced at Komipharm International Co., Ltd.Korea
Yong-kwan Won, Abebe M Aga, Birhanu Hurisa, Ju Hun Kim, Hailu Lemma, Su Jin Lee, Choong soon Yun, Ho kyoung Jung, Seong Cheol Moon, O soo 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 132 261-262 (2 pages)
fluorescent antibody virus neutralisation test (FAVNtest) for the quantitation of rabies-neutralising antibody. J Immunol Methods 212: 79-87. P-073 In Vitro Screening of Probiotic Potentials of Bacteria Isolated From Korean Fermented Foods and Wild Birds Sol Jeong 1, Gyeong-Bin Gwon 1, Kyu-Jik Kim 1, Woo-Taek Hong 1, Yu-Jin Kim 1, Jung-Hoon Kwon 1, Chang-Seon Song *1 1Avian Disease Laboratory,... -
Brucella abortus gene, BruAb2_1031, associated with enhanced intracellular survival in RAW 264.7 cells
Myung-hwan Jung, Soo Jin Shim, Young Bin Im, Woo Bin Park, Han Sang Yoo 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 37 102-103 (2 pages)
virus replicon expressing green fluorescent protein Hyun-Woo Moon 1, Soo-Jeong Park 1, Haan-Woo Sung 1, Hyuk-Moo Kwon *1 1Laboratory of Veterinary Microbiology, College of Veterinary Medicine, Kangwon National University, Chuncheon, Republic of Korea Introduction: Hepatitis E virus (HEV) is associated with endemic or epidemic forms of hepatitis E in humans. HEV is transmitted via water-borne... -
An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus
Ji Eun Yu, In-Ohk Ouh, Hyeonjeong Kang, Hye-young Lee, Kwang-Myun Cheong, In-Soo Cho, Sang-Ho Cha 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 7 519-527 (9 pages)
12. Joo HS, Park BK, Dee SA, Pijoan C. Indirect fluorescent IgM antibody response of pigs infected with porcine reproductive and respiratory syndrome syndrome virus. Vet Microbiol 1997, 55, 303-307. 13. Kameyama K, Sakoda Y, Tamai K, Igarashi H, Tajima M, Mochizuki T, Namba Y, Kida H. Development of an immunochromatographic test kit for rapid detection of bovine viral diarrhea virus antigen.... Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most important infectious diseases causing serious economic loss in the swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical for PRRS control. The immunochromatographic strip test (ICST) is a rapid and convenient type of immunoassay. In this study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus... -
Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 4 350-357 (8 pages)
by the fluorescent dye TAMRA (6-carboxytetramethylrhodamine) at the 3´ end. All primers and probes were purchased from Sigma-Aldrich (USA) or Integrated DNA Technologies. Table 1 lists the primers and probes used in this study. Extraction of nucleic acids Viral nucleic acids were extracted by using High Pure Viral Nucleic Acid Kit (Roche Diagnostics, Germany). The QIAamp Fast DNA Stool Mini... Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain...