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Reduction of long-term potentiation at Schaffer collateral-CA1 synapses in the rat hippocampus at the acute stage of vestibular compensation
GyoungWanLee, JaeHyoKim, MinSunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.4 8 423-428 (6 pages)
using stainless steel blades in an ice-cold sucrose cutting solution (in mM) (sucrose 110, NaCl 60, NaHCO 3 28, NaH2PO4 1.25, KCl 3, MgSO4 7, CaCl2 0.5, glucose 5, sodium ascorbate 0.6), and then transferred to a chamber (100 ml) containing aCSF at 25°C. In each slice, the hippocampus was selectively isolated from surrounding brain tissue using a surgical knife under a surgical microscope.... Vestibular compensation is a recovery process from vestibular symptoms over time after unilateral loss of peripheral vestibular end organs. The aim of the present study was to observe time-dependent changes in long-term potentiation (LTP) at Schaffer collateral-CA1 synapses in the CA1 area of the hippocampus during vestibular compensation. The input-output (I/O) relationships of fEPSP amplitudes and LTP induced by theta burst stimulation to Schaffer’s collateral commissural fibers were... -
Streptozotocin Diabetes Attenuates the Effects of Nondepolarizing Neuromuscular Relaxants on Rat Muscles
LinaHuang, DanChen, ShitongLi 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.6 3 461-468 (8 pages)
days after injection, blood sugar was measured twice in series by cutting tails. Successful models were de- termined if the blood sugar values were above 16.7 mmol/L. The rats with blood sugar tested below 16.7 mmol/L were rejected from the study. After modeling successfully, the diabetic rats and the age-matched controls were randomly divided into two subgroups, diabetic 4 weeks group(dia 4... The hypothesis of this study was that diabetes-induced desensitization of rat soleus (SOL) and extensor digitorum longus (EDL) to non-depolarizing muscle relaxants (NDMRs) depends on the stage of diabetes and on the kind of NDMRs. We tested the different magnitude of resistance to vecuronium, cisatracurium, and rocuronium at different stages of streptozotocin (STZ)-induced diabetes by the EDL sciatic nerve-muscle preparations, and the SOL sciatic nerve-muscle preparations from rats after 4 and... -
Voltage-dependent Ca2＋ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach
YoungChulKim, HikaruSuzuki, Wen-XieXu, HikaruHashitani, WoongChoi, Hyo-YungYun, Seon-MeePark6, SeiJinYoun6, Sang-JeonLee, SangJinLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.6 6 323-330 (8 pages)
controlled at 36 oC for conventional sharp electrode recording, 32 oC for conventional whole-cell voltage clamp. Tissue preparation for sharp electrode recording The excised stomach was opened by cutting along the small curvature in Krebs solution. The mucosal layer was removed by cutting with fine scissors, and smooth muscle tissues were isolated from the antral region. Under a dis- secting... The properties of voltage dependent Ca2＋ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min−1, respectively, in the presence of 1</SUP>ՌM nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These... -
Effect of Propofol, an Intravenous Anesthetic Agent, on KATP Channels of Pancreatic β-cells in Rats
EunJeePark, Dae-KyuSong, JaeKyuCheun, JungInBae, Won-KyungHo, YungEEarm 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.1 4 25-31 (7 pages)
sa1ine) was injected through the abdominal aorta after cutting the inferior vena cava since this stain is selective for islet tissue (Gray et al， 1983) Collagenase (1.2 mg/ml Hank’s balanced salts solu tion) solution was perfused into the pancreatic ducts retrogradely through the common bile duct. The dissected pancreas was incubated in 370C water bath for 33 min πle dispersion 01 islet... manner. The half-maximal inhibition dose (ED50) was 48.6⁑8.4μM and the Hill coefficient was 0.73⁑0.11. Single channel conductance calculated from the slope of the relationship between single channel current and pipette potential (20∼100 mV) was not significantly altered by propofol (control: 60.0⁑2.7 pS, 0.1 mM propofol: 58.7⁑3.5 pS). However, mean closed time was surely increased. Above results indicate that propofol blocks the KATP channels in the... -
Role of Na+Ca2+ Exchange in the Control of Contractility in Rabbit Basilar Arterial Smooth Muscle
Kim Eui-Yong, Han Jin 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 6 159-168 (10 pages)
AND METHODS Preparation of basilar arterial circular muscle Rabbits of either sex weighing about 2 kg were stunned by a sudden blow to the hind neck and killed by exsanguination via the cutting of both carotid arteries. A basilar arterial segment was then removed ., and transferred to a dissection bath con.taining oxygenated Tyrode solution. Circular muscle fibers with a size of 4 mm in... The contraction of rabbit basilar artery was examined as a function of changes in the Na+ electrochemical gradient in order to determine the contribution of Na+/Ca2+ exchange to the modulation of contractility. Ouabain (10-5 M) or K+-free Tyrode solution caused an increase in tonic tension even in the presence of a Ca2+ channel blocker (10-6 M verapamil) and an α-receptor blocker (10-5 M phentolamine). After treatment with ouabain (10-5 M), contractions were augmented by reduction of external... -
A Study on the Na+/Ca2+ Exchange Mechanism in the Smooth Muscle of Guinea-pig Stomach
Kim, Eui-Yong, Han, Jin, Kim, Ki-Whan 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 6 55-68 (14 pages)
blow on the hind neck and exsanguinated by cutting both the carotid arteries. The stomach was extracted quickly and transferred into a chamber containg oxygen- ated Tyrode solution. The antral part was isolated by removing the other parts with a scissors. The antral part was cut in the longi- ..PAGE:3 -A Study on the Na+ /Ca'+ Exchange Mechanism in the Smooth Muscle of Guinea-pig Stomach-... sigmoid pattern. The slope of straight line from Hill plot was 2.7. 6. In parallel with the increase of extracellular Ca2+ concentration, the amplitude of contracture increased dose dependently and was maximum at 8 mM Ca2+-Tyrode solution. 7. The relationship between extracellular Ca2+ concentrations and the magnitude of contractures showed hyperbolic pattern. The slope of straight line from Hill plot was 1.1. From the above results, it is suggested that Na+/Ca2+ exchange mechanism exists in the... -
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 4 123-131 (9 pages)
of seed cutting (Manzer and Genereux, 1981). Improper sanitation methods that are used during cool temperature storage and in planting machinery can also spread the pathogen to new seed pieces (Bonde, 1942). Thus, Cms cells are easily transferred to new sites, resulting in the classification of this pathogen as highly contagious. Because of the highly contagious nature of the ring rot... Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced... -
Preparation and application of reduced graphene oxide as the conductive material for capacitive deionization
Ayu Tyas Utami Nugrahenny, Jiyoung Kim, Sang-Kyung Kim, Dong-Hyun Peck, Seong-Ho Yoon, Doo-Hwan Jung 한국탄소학회 Carbon Letters 7 Pages
한국탄소학회 Carbon Letters 2014, Vol.15 No.1 5 38-44 (7 pages)
cutting the cast electrode into a piece 10 × 10 cm 2 with an additional area (3 × 5 cm 2) left for a connectivity space. A hole with a diameter of 8 mm was made for electrolyte fow in the middle of the electrode. For comparison, we also made an electrode with graphite as the conductive material (AC-Gr) with the same composition as AC- RGO. An electrode with no added conductive material (AC)... This paper reports the effect of adding reduced graphene oxide (RGO) as a conductive material to the composition of an electrode for capacitive deionization (CDI), a process to remove salt from water using ionic adsorption and desorption driven by external applied voltage. RGO can be synthesized in an inexpensive way by the reduction and exfoliation of GO, and removing the oxygen-containing groups and recovering a conjugated structure. GO powder can be obtained from the modification of Hummers... -
수변지역 소규모 개발사업의 유형별 환경평가 기준에 관한 연구
주용준, 사공희 한국습지학회 한국습지학회지 8 Pages
한국습지학회 한국습지학회지 2016, Vol.18 No.3 2 218-225 (8 pages)
- Disparate landscape caused by slope cutting and retaining wall - Damage of good vegetation and intimidate of major living organisms - Water quality and hydroecological impact caused by non-point source pollution - Life environmental worsen caused by noise and air pollution 12 AIb - Excessive topography damage caused by site clearance of steep slope area - Disparate landscape caused by slope... 수변지역은 하천의 수질 및 수생태계의 보전과 수변경관의 훼손 방지 특히, 안정적인 상수원의 확보를 위하여 다양한 정책과 계획이 수립되어 왔다. 그럼에도 불구하고 상수원보호구역과 수변구역 등 특정지역을 제외한 수변지역은 소규 모 개발사업이 무분별하게 입지하게 되면서 하천의 수질 및 경관을 훼손되고 있다. 본 연구에서는 사업유형별로 동?식 물, 지형?지질, 경관, 수질 등의 측면에서 예상되는 환경영향을 분석하고, 환경적 영향 정도에 따라 3-12점까지의 환 경점수를 부여하여 중점검토사업과 일반검토사업, 간이검토사업... -
농촌지역 소하천의 제방형태에 따른 식생특성 분석연구
김미희, 강방훈, 공민재, 정명철, 손진관 한국습지학회 한국습지학회지 11 Pages
한국습지학회 한국습지학회지 2015, Vol.17 No.4 5 359-369 (11 pages)
식생다 양성을 위한 제고방안을 알아보았다. Table 2. The assessment items of NRCS(USDA, 2012) Assessment Items 1. Stream Incisement, 2. Lateral Cutting, 3. Stream Balance, 4. Deep, Binding Rootmass, 5. Riparian/Wetland Vegetative Cover, 6. Noxious Weeds, 7. Undesirable Plants, 8. Woody Species Establishment, 9. Browse Utilization, 10. Riparian Area/Floodplain Characteristics * Grade :... 하천생태계는 환경, 생태적 중요한 가치를 지니는 공간이지만 최근 다양한 위협에 처해있다. 다양한 복원이 시도되고 있어 유형에 따른 식생특성 발달 정도를 평가하였다. 제방형태 중 자연형제방이 42∼45 종류, 인공형제방이 23∼38 종류로 확인되어 자연형제방이 인공형제방에 비해 식생의 다양함을 확인하였다. 더불어 자연형 제방에서 인공형 보다 꿀풀과와 사초과가 발달함을 확인하였다. 일년생식물의 출현빈도는 인공형제방 지역이 낮은 빈도임을 확인 하여 하천정비 시 일년생식물이 도입 될 수 있도록 서식공간을 넓게 조성 할...