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Oocyte maturation under a biophoton generator improves preimplantation development of pig embryos derived by parthenogenesis and somatic cell nuclear transfer
Joohyeong Lee , Hyeji Shin , Wonyou Lee , Seung Tae Lee , Geun-shik Lee , Sang-hwan Hyun , Eunsong Lee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 2호 3 89-95 (7 pages)
gonadotrophic hormones for 22 h, after which they were matured in hormone-free medium for an additional 22 hr. Next, IVM oocytes were induced for parthenogenesis (PA) or provided as cytoplasts for somatic cell nuclear transfer (SCNT). Treatment of oocytes with biophoton energy during IVM did not improve cumulus cell expansion, nuclear maturation, intraoocyte glutathione content, or mitochondrial... -
A Retrospective Study on the Risk Factors and the Effect of Higher Somatic Cell Count in Milk on Reproductive Performance in Dairy Cows
서보성, 신은경, 정재관, 강현구, 김일화, Seo. Bo-Sung, Shin. Eun-Kyung, Jeong. Jae-Kwan, Kang. Hyun-Gu, Kim. Ill-Hwa 한국임상수의학회 Journal of veterinary clinics 6 Pages
한국임상수의학회 Journal of veterinary clinics 2014, Vol.31 No.4 272-277 (6 pages)
본 연구는 젖소에서 분만 후 비유초기 체세포 증가가 번식효율에 미치는 영향에 대하여 조사하였다. 774두의 젖소 자료를 분만 후 3개월 평균 체세포지수에 따라 T1(3.0 미만, n = 521), T2(3.0 이상 4.0 미만, n = 113) 및 T3(4.0 이상, n = 140) 군으로 구분하였다. 자궁내막염의 발생위험율(odds ratio)은 T1군에 비해 T2군 및 T3군이 각각 1.6배(p < 0.05) 및 3.2배(p < 0.0001) 증가하였다. 분만 후 150일까지 첫 수정율은 T1군에 비해 T3군이 낮았으나(hazard ratio [HR] = 0.76, p < 0.01), 분만 후 첫수정 수태율은 각... -
Klotho plays a critical role in clear cell renal cell carcinoma progression and clinical outcome
Ji-HeeKim, Kyu-HeeHwang, SayamaaLkhagvadorj, JaeHungJung, HyunChulChung, Kyu-SangPark, , InDeokKong, MinseobEom, Seung-KuyCha 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.3 9 297-304 (8 pages)
Lindau ( VHL) gene, somatic mutation of which occurs in most case of ccRCC [11,17,22]. E3 ligase VHL protein functions as tumor suppressor via inhibiting hypoxia-inducible factor-1) whose regulation by VHL is crucial for ccRCC tumorigenesis. Additonally, components of PI3K/Akt pathway, major downstream of IGF-1R, have been frequently mutated in ccRCC [17]. Multiple studies demonstrated that... clear cell renal cell carcinoma (ccRCC). Altered expression and/or activity of growth factor receptor have been implicated in ccRCC development. Although Klotho suppresses a tumor progression through growth factor receptor signaling including insulin-like growth factor-1 receptor (IGF-1R), the role of Klotho acting on IGF-1R in ccRCC and its clinical relevance remains obscure. Here, we show that Klotho is favorable prognostic factor for ccRCC and exerts tumor suppressive role for ccRCC through... -
Ultrasonographic ovarian dynamic, plasma progesterone, and non-esterified fatty acids in lame postpartum dairy cows
Pedro Melendez, Veronica Gomez, Hans Bothe, Francisco Rodriguez, Juan Velez, Hernando Lopez, Julian Bartolome, Louis Archbald 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 16 462-467 (6 pages)
ovulation, reproductive hormone concentrations and estrus behavior in dairy cows. Theriogenology 2011, 76 658-668. 27. Morris MJ, Walker SL, Jones DN, Routly JE, Smith RF, Dobson H. Influence of somatic cell count, body condition and lameness on follicular growth and ovulation in dairy cows. Theriogenology 2009, 71, 801-806. 28. Nanda AS, Dobson H, Ward WR. Relationship between an increase... The objective of this study was to compare ovulation rate, number of large ovarian follicles, and concentrations of plasma progesterone (P4) and non-esterified fatty acids (NEFA) between lame (n = 10) and non-lame (n = 10) lactating Holstein cows. The study was conducted in an organic dairy farm, and cows were evaluated by undertaking ultrasonography and blood sampling every 3 days from 30 days postpartum for a period of 34 days. Cows which became lame during the first 30 days postpartum... -
Effect of canine mesenchymal stromal cells overexpressing heme oxygenase-1 in spinal cord injury
Seung Hoon Lee, Yongsun Kim, Daeun Rhew, Ahyoung Kim, Kwang Rae Jo, Yongseok Yoon, Kyeung Uk Choi, Taeseong Jung, Wan Hee Kim, O 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 15 377-386 (10 pages)
Conflict of Interest The authors declare no conflict of interests. References 1. Andrews EM, Tsai SY, Johnson SC, Farrer JR, Wagner JP, Kopen GC, Kartje GL. Human adult bone marrow-derived somatic cell therapy results in functional recovery and axonal plasticity following stroke in the rat. Exp Neurol 2008, 211, 588-592. 2. Bains M, Hall ED. Antioxidant therapies in traumatic brain and spinal... 7 weeks after transplantation. Histopathological results showed fibrotic changes and microglial cell infiltration were significantly decreased in the MSC-HO-1 group. Immunohistochemical (IHC) results showed significantly increased expression levels of HO-1 and neuronal markers in the MSC-HO-1 group. Western blot results showed significantly decreased expression of tumor necrosis factor-alpha, interleukin-6, cycloogygenase 2, phosphorylated-signal transducer and activator of transcription 3, and... -
Learning, memory and exploratory similarities in genetically identical cloned dogs
Chi Won Shin, Geon A Kim, Won Jun Park, Kwan Yong Park, Jeong Min Jeon, Hyun Ju Oh, Min Jung Kim, Byeong Chun Lee 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 16 563-567 (5 pages)
Keywords: cloned dogs, exploratory, learning and memory, similarity, somatic cell nuclear transfer Introduction It is well known that cloned animals with identical genomes can be produced by somatic cell nuclear transfer (SCNT). SCNT can be performed to preserve endangered species [23], produce transgenic models [12,17] and propagate dogs with elite abilities [22]. For propagation of dogs with... Somatic cell nuclear transfer allows generation of genetically identical animals using donor cells derived from animals with particular traits. To date, few studies have investigated whether or not these cloned dogs will show identical behavior patterns. To address this question, learning, memory and exploratory patterns were examined using six cloned dogs with identical nuclear genomes. The variance of total incorrect choice number in the Y-maze test among cloned dogs was significantly lower... -
Reproductive ability of a cloned male detector dog and behavioral traits of its offspring
Ji Hyun Lee, Geon A Kim, Rak Seung Kim, Jong Su Lee, Hyun Ju Oh, Min Jung Kim, Do Kyo Hong, Byeong Chun Lee 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 19 407-411 (5 pages)
Korean Natural Monument, using somatic cell nuclear transfer. J Vet Med Sci 2009, 71, 1217-1220. 10. Jang G, Hong SG, Oh HJ, Kim MK, Park JE, Kim HJ, Kim DY, Lee BC. A cloned toy poodle produced from somatic ..PAGE:5 Behavioral traits of Labrador Retriever puppies derived from cloned dog 411 www.vetsci.org cells derived from an aged female dog. Theriogenology 2008, 69, 556-563. 11. Jang G, Kim... In 2007, seven detector dogs were produced by somatic cell nuclear transfer using one nuclear donor dog, then trained and certified as excellent detector dogs, similar to their donor. In 2011, we crossed a cloned male and normal female by natural breeding and produced ten offspring. In this study, we investigated the puppies’ temperaments, which we later compared with those of the cloned parent male. The results show that the cloned male had normal reproductive abilities and produced healthy... -
Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
agar for all organisms along with McConkey and EMB agar for E. coli . The samples were also subjected to Gram staining and microscopy evaluation [29]. The healthy cows were identified based on somatic cell counts less than 200,000 cells/mL in milk measured using a Fossomatic 5000 instrument (Foss, Denmark). All samples were washed with PBS to remove the milk and debris, and then stored in... Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel... -
Development of in vitro produced porcine embryos according to serum types as macromolecule
Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 11 315-321 (7 pages)
gonadotropin, in vitro fertilization parthenogenesis, porcine, serum Introduction Oocyte maturation is one of the important stages for successful production of in vitro fertilization (IVF) and somatic cell nuclear transfer embryos [1]. Because in vitro maturation (IVM) is not as efficient as that of in vivo, many studies are needed to determine the optimal culture media compositions needed... higher in the PGS group, with no significant differences in the blastocyst formation. However, when GTH was supplemented into the IVM media, there were no significant differences among the four groups in the cleavage rates, development rates of the blastocyst, and cell number of the blastocyst after PA and IVF. In conclusion, PGS is an efficient macromolecule in porcine IVM, and GTH supplementation of the IVM media is beneficial when PS is used as macromolecule, regardless of its origin. -
Identification of differentially expressed genes in gauze-exposed omentum of dogs using differential display RT-PCR
Md. Mizanur Rahman, Yongsun Kim, Ye-Eun Byeon, Hak-Hyun Ryu, Wan Hee Kim, Mahmuda Umme Rayhan, Oh-Kyeong Kweon* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 8 167-173 (7 pages)
E, Veeramachaneni DNR. Testicular germ cell tumors in dogs are predominantly of spermatocytic seminoma type and are frequently associated with somatic cell tumors. Int J Androl 2011, 34 (4 Pt2), e288-295. 3. Chandra A, Srivastava RK, Kashyap MP, Kumar R, Srivastava RN, Pant AB. The anti-inflammatory and antibacterial basis of human omental defense: selective expression of cytokines and... Molecular mechanisms governing peritonitis caused by the presence of aseptic gauze have remained unclear. To identify the genes involved, sterile gauze-exposed omentum was collected at 0, 6, 12, 24, and 48 h intervals, and analyzed by differential display RT(reverse transcription)-PCR. Among over 1,200 bands, 230 bands were found differentially expressed. These bands represented the fragment sizes of approximately 200 to 1,500 bp. The eight fragments were expressed differentially in the...