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전체 선택해제
총 53건 검색
본문검색 결과만 보기
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Fuel Spiking Test for the Surge Margin Measurement in a Gas Turbine Engine
Lee. Jin-Kun, Lee. Kyung-Jae, Ha. Man-Ho, Kim. Chun-Taek, Yang. Soo-Seok, Lee. Dae-Sung 한국추진공학회 한국추진공학회지 7 Pages
한국추진공학회 한국추진공학회지 2004, Vol.8 No.2 18-24 (7 pages)
가스터빈엔진 압축기의 서지마진을 구하기 위하여 연료 돌출 시험을 수행하였다. 본 시험에 사용된 연료 돌출 신호는 엔진 제어기 신호에 중첩되어 연료 밸브를 제어하는 데 사용되었으며 신호 중첩을 위해 연료 제어기와 함수 발생기로 구성된 보조시스템이 사용되었다. 한국항공우주연구원의 고공엔진시험설비에서 실제 엔진 시험이 이루어졌으며 예비 시험결과, 연료 돌출 신호는 연료 라인과 압축기 토출부에서의 압력 신호와 잘 일치하였다. 이에 따라 특정 속도에서의 서지점을 측정하기 위한 시험이 수행되었으며 시험 결과 연료... -
Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay
Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 5 143-150 (8 pages)
can be extracted more easily from the solid matrices of animal feed with water than organic solvents like methanol. The Ab-coated ELISA was validated by spiking feed with DON at concentrations ranging from 0 to 1,000 μg/kg ..PAGE:6 148 Hyuk-Mi Lee et al. Table 3. Recovery of DON from spiked feed samples with the DON-mAb coated ELISA DON (μg/kg) Recovery rate (%)* CV (%) Spiked Detected* 0... Monoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative DON calculation ranged from 50 to 4,000 ng/mL for DON-Ab coated ELISA and from 25 to 500 ng/mL for DON-Ag coated ELISA. 50% of inhibitory concentration values of DON, HT-2, 15-acetyl-DON, and nivalenol were 23.44, 22,545, 5,518 and 5,976 ng/mL based on the DON-Ab coated ELISA.... -
Quantitation of meloxicam in the plasma of koalas (Phascolarctos cinereus) by improved high performance liquid chromatography
Benjamin Kimble, Kong Ming Li, Merran Govendir* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 2 7-14 (8 pages)
by spiking the blank pooled koala plasma with the appropriate amount of working standard MEL solutions and stored at 20 o C. Calibration and sample preparation Plasma calibration standards (ranging from 10 to 1,000 ng/mL) were freshly prepared by spiking 250 μL of blank pooled plasma pre-thawed at room temperature with 50 μL of the MEL working standard solutions. For preparation of... An improved method to determine meloxicam (MEL) concentrations in koala plasma using reversed phase high performance liquid chromatography equipped with a photo diode array detector was developed and validated. A plasma sample clean-up step was carried out with hydrophilic- lipophilic copolymer solid phase extraction cartridges. MEL was separated from an endogenous interference using an isocratic mobile phase [acetonitrile and 50 mM potassium phosphate buffer (pH 2.15), 45：55 (v：v)] on a... -
Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay
Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 8 143-149 (7 pages)
positive control was prepared by spiking 25 g of the sample homogenate with approximately 10 8 CFU/mL of SE. For negative controls, uninoculated food (25 g) and sterilized PBS (25 mL) were also prepared. After overnight stabilization at 4 o C, all 22 samples, including the control samples, were mixed with 225 mL BPW followed by homogenization in a laboratory Stomacher blender for 30 sec and... The best enrichment broth and DNA extraction scheme was determined for rapid and sensitive detection of Salmonella Enteritidis in steamed pork using real-time PCR. The inhibitory effect of commonly used Salmonella enrichment broths, Rappaport-Vassiliadis (RV) and Muller-Kauffmann tetrathionate with novobiocin (MKTTn), on real-time PCR was confirmed. The inhibition of PCR was statistically significant (p ＜ 0.05) in RV and MKTTn, as compared with buffered peptone water (BPW) or phosphate-buffered... -
A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats
Su Hwa Lee, Byeong Yeal Jung, Nabin Rayamahji, Hee Soo Lee, Woo Jin Jeon, Kang Seuk Choi, Chang Hee Kweon, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 7 43-51 (9 pages)
Typhimurium and 0.65 ± 0.07 to 2.66 ± 0.05 log10 CFU/ml for S. Enteritidis) were spiked into beef and pork. The meat samples were taken for the multiplex real-time PCR either immediately after spiking (pre-enrichment condition) or put in incubation at 37 o C for 18 h after spiking (post-enrichment condition). When 0.54 ± 0.09 log 10 CFU/ml of S. Typhimurium and 0.65 ± 0.07 log10 CFU/ml ... Salmonella (S.) Typhimurium and S. Enteritidis are the major causative agents of food-borne illnesses worldwide. Currently, a rapid detection system using multiplex real-time polymerase chain reaction (PCR) has been applied for other food-borne pathogens such as Escherichia coli, Staphylococcus aureus and Streptococcus spp. A multiplex real-time PCR was developed for the simultaneous detection of Salmonella spp., especially S. Typhimurium and S. Enteritidis, in beef and pork. For the specific... -
Evaluation of a Bacillus stearothermophilus tube test as a screening tool for anticoccidial residues in poultry
Anakalo Shitandi, Aila Oketch, Symon Mahungum 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 14 177-180 (4 pages)
or 5 ml of the negative-control solution. Tissue spiking Solutions containing 0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 2.0, and 4.0g/ml of sulfamethazine- and furazolidone-contaminated liver and kidney tissues were prepared using appropriate working solutions and blank tissue. Liver tissue was contaminated with sulfamethazine in a pH 8.0 phosphate buffer. Solutions of 1, 2, 4, 6, 8, 10, 12 and 14g/ml... A Bacillus stearothermophilus var. calidolactis C953 tube test was evaluated for its ability in detecting the residue of selected anticoccidial drugs in poultry, specically sulfamethazine, furazolidone, and amprolium. Various concentrations of each drug were injected into chicken liver and kidney tissues and these tissues were tested to determine the drug detection limits for each drug. The detection limit was defined as the drug concentration at which 95% of the test results were interpreted as... -
Pharmacokinetics, tissue residue and plasma protein binding of ofloxacin in goats
Himangshu Baruah, Dulal Chandra Roy, Rohini Kumar Roy, Hirendra Nath Khonikor 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 2 97-101 (5 pages)
by spiking blank plasma with standard parent compound at different concentration ranging from 0.025 to 20g/ml and extracted by liquid phase extraction as described above. The plasma concentration of ofloxacin in the samples were determined by comparing the detector response for the drug in the sample with the corresponding standards (Fig. 1). The homogenized tissue samples were subjected to liquid... Ofloxacin was administered to six male goats intravenously (5 mg/kg) to determine its kinetic behavior, tissue residue, in vitro plasma protein binding and to compute a rational dosage regimen. The concentration of ofloxacin in plasma and tissue samples collected at prescheduled time were estimated by using HPLC. The pharmacokinetic parameters were determined by non-compartmental model and plasma protein binding was estimated by equilibrium dialysis technique. The therapeutic concentration... -
Physiology,Pharmacology,Chemistry : Comparison of Linear and non-Linear equation for the calibration of roxithromycin analysis using Liquid chromatography/mass spectrometry
Jong Hwan Lim , Hyo In Yun 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2010, 제 50권 제 1호 2 11-17 (7 pages)
(ten times LLOQ), middle level and high level (the upper limit of quantitation limit, ULOQ). QC samples were prepared daily by spiking different plasma samples to produce a final concentration equivalent to 0.01, 0.1, 5 and 10g/mL of roxithromycin. Sample preparation of roxithromycin was followed by the method of Lim et al. [10, 11] with some modifications. Briefly, to 200L of spiked plasma, 10 L... -
해부,생화학,약리학,독성학 : 수입 식육의 안전성 확보를 위한 polychlorinated dibenzo-p-dioxins와 dibenzofurans 분석
김미경, 김동규 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2008, 제 48권 제 1호 6 39-47 (9 pages)
factor, TEF)를 지닌 2,3,7,8-tetrachlo- rodibenzo-p-dioxin 등 17종 PCDD/Fs 을 분석대상 물질로 선정하였다(Table 1). 동위원소표지된 표준물질(labeled compound spiking solution, LCS), 정제용 표준물질(cleanup standard solution, CSS), 기기보정용 표준물질(internal standard solution, ISS)로 사용된 PCDD/Fs는 Wellington Laboratories(Canada) 제품을 사용하였고 검량선 작성용 표 준물질(cal ibration standard... -
소금 첨가에 따른 도라지 발효 특성과 미생물 변화 및 항비만 효능 평가
신나래, 임수경, 김호준 한방비만학회 대한한방비만학회지 10 Pages
한방비만학회 대한한방비만학회지 2018, 제 18권 제 2호 2 64-73 (10 pages)
Eur J Phycol. 1996 ; 31(1) : 97-105. ..PAGE:10 신나래 외: 소금 첨가에 따른 도라지 발효 특성과 미생물 변화 및 항비만 효능 평가 www.jkomor.org 73 18. Ehrhardt DW, Wais R, Wais SR. Calcium spiking in plant root hairs responding to Rhizobium nodulation signals. Cell. 1996 ; 85(5) : 673-754. 19. Cha SK. Probiotic microorganisms and lactic acid bacte- rial foods. J Microbiol. 2000 ; 26(2) : 13-21. 20....