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Netrin-1 Specifically Enhances Cell Spreading on Fibronectin in Human Glioblastoma Cells
HyunKyoungLee, InAeSeo, YoonKyungShin, SangHwaLee, Su-YoungSeo, DukJoonSuh, HwanTaePark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.5 2 225-230 (6 pages)
In conclusion, we found that netrin-1 affected glioma cell spreading on fibronectin, possi bly by binding to integrin α v. It is, therefore, likely that netrin employs two different receptor systems, classical netrin receptors and integrins, to perform its diverse cellular functions. The interaction be- tween netrins and integrins seems to play an important role in the tumor cell adhesion ,... Netrins are secreted molecules and involved in axon guidance, cell migration and tumor development. Recent studies revealed that netrins perform novel functions in such processes as epithelial development and angiogenesis without operating through the classical netrin receptors, DCC (Deleted in Colorectal Cancer) and Unc5h. In the present study, we investigated the roles of netrin-1 and its receptors in cell spreading of human glioblastoma cells, and found that netrin-1 haptotactically enhanced... -
Media Landscape without Apple: A Workshop for Critical Awareness of Alternative Media Infrastructure
Mizukoshi Shin 경인교육대학교 교육연구원 The Journal of Education 18 Pages
경인교육대학교 교육연구원 The Journal of Education 2020, Vol.3 No.2 5 77-94 (18 pages)
such as artificial intelligence (AI) and the Internet of Things (IoT) have been rampant worldwide. This signifies that digital networks have been spreading into every corner of society; daily life, work, education, consumption, and even life and death is mediated. Every state of affairs in the world has come to be mediated by media. Thus, the infrastructural aspect of media has been attracting... Until the 2000s, media literacy evolved while centering around the application of critical thinking of contents such as TV programs and the exchanges on social media. However, unlike television, messages on social media are inseparable from the infrastructure that supports them, as the nature of the infrastructure frames the contents and the communication phenomenon. When Google, Apple, Facebook, and Amazon are so visible in our daily lives, we believe it is necessary to examine and promote... -
Prevention and Intervention of Adolescents’ Maladaptive Internet use
Oh Yeon Ju 경인교육대학교 교육연구원 The Journal of Education 15 Pages
경인교육대학교 교육연구원 The Journal of Education 2019, Vol.2 No.2 1 1-15 (15 pages)
2016 (Integrated) Withdrawal Withdrawal Withdrawal Salience Tolerance Tolerance Tolerance Self-control failure Disturbance of Adaptive Functions Disturbance of Adaptive Functions Disturbance of Adaptive Functions Serious consequences Deviate Behavior - - - Virtual Interpersonal Relationship Virtual Interpersonal Relationship Pursuit of virtual world Addictive Automatic Thought - -... This article presents the characteristics of addiction, cognitive behavior, and social cognitive perspectives, which are the mainstream views of maladaptive Internet use. As the academic branch dealing with mental health, such as psychology and psychiatry, first raised the issue of maladaptive Internet use and continued research, views in the context of mental health, such as addiction and cognitive behavior perspectives, have become mainstream. The mental health perspective, however, shows... -
Examples of Maker Education using Digital Logic Circuits and Arduino Input / Output Systems
Hur Kyeong 경인교육대학교 교육연구원 The Journal of Education 12 Pages
경인교육대학교 교육연구원 The Journal of Education 2018, Vol.1 No.2 5 65-76 (12 pages)
competence. In addition, computers, digital devices, and 3D printers are becoming popular, and innovative teaching methods are needed. Maker education is derived from the maker movement that is spreading in the United States under the influence of DIY (Do It Yourself) movement. Maker education indicates that students demonstrate their creativity by solving problems, creating new things, and... Maker education is the process of implementing what is learned through SW education by using tools such as Arduino, Robot, 3D printer, VR, etc., and making it into reality. In other words, maker education is a teaching and learning method that realizes SW education. In this study, we propose a case study on maker education based on computational thinking. To this end, we propose a training case for developing devices for real life using Arduino input / output computing systems and digital logic... -
Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 7 511-517 (7 pages)
on their known functions and commercial antibodies. Briefly, equal amounts (30 μg) of protein samples from the day 5 post-infection ..PAGE:3 Proteomic analysis of chicken PBMCs after infection by NDV 513 Fig. 2. Representative 2- dimensional electrophoresis (DE) gel images obtained for peripheral blood mononuclear cells (PBMCs) from chickens that were infected by NDV or mock-infected. (A)... Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78... -
Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)
Geewook Shin, Hyungjun Lee, K. J. Palaksha, Youngrim Kim, Eunyoung Lee, Yongseung Shin, Eunggoo Lee, Kyungdae Park, Taesung Jung 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 14 293-295 (3 pages)
responses involve the production of specific immunoglobulins (Igs) by the host following antigen stimulation [19]. The Igs produced protect the host by removing the pathogen or preventing it from spreading into the blood or organs. Mammals possess five classes of Ig heavy chains: IgM, IgG, IgA, IgE, and IgD [19]. Fish, on the other hand, have been reported to have four Ig heavy chain isotypes at... The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG affinity columns. These three different ligands were found to possess high affinity for black rockfish serum Ig. All of the Igs purified eluted at only 0.46 M NaCl concentration in anion exchange column chromatography and consisted of two bands at 70 kDa and 25 kDa in SDS-PAGE;... -
Changes in patterns of antimicrobial susceptibility and class 1 integron carriage among Escherichia coli isolates
Sang Gyun Kang, Deog Yong Lee, Sung Jae Shin, Jeong Min Ahn, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 5 201-205 (5 pages)
a new mechanism for spreading genes of resistance among Gram negative bacteria and act as natural expression vectors supplying a common promoter to a mobile gene cassette containing various antibiotic-resistance genes. The essential components of the integron are found within the 5'- conserved segment of the element and include an integrase gene intI, which encodes a site-specific recombinase, an... The worldwide use of antimicrobials in different fields has created enormous pressure for the selection of resistance among opportunistic bacterial pathogen. One hundred four E. coli isolates were collected and identified from swine with diarrhea in Korea during the period of 2002. The isolates showed highly resistant to streptomycin (99.0%), tetracycline (97.1%), neomycin (91.3%)and carbenicillin (84.6%)in antimicrobial susceptibility test. Moreover, all of the isolates showed multiple... -
Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity
Sang-Hyun Kim, Yong-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 5 119-124 (6 pages)
in the double msbB mutant whose outer membrane (OM) functions are compromised by alterations of lipid A species [15,17], compared to that of wild type and/or two single msbB1 and msbB2 mutants (data not shown). The CR+ bacteria grown in EMEM without containing FCS were slightly more aggregative than those grown in EMEM(-) containing 10% FCS (data not shown). The clump-formation was consistent with... Escherichia coli (E. coli) has ability to express thin aggregative fimbriae, known as curli, on the cell surface. Previously, a few example of curli expression in serogroup O157:H7 of enterohemorrhagic E. coli (EHEC) were reported, compared to other E. coli groups. However, significance of curliation in the EHEC pathobiology has not been described well in the literature. A highly curliated O157:H7 strain was used in this study in order to elucidate role of curliation in EHEC adherence to... -
Physiological understanding of host-microbial pathogen interactions in the gut
Sei Jung Lee , Sang Ho Choi , Ho Jae Han 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 10 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 2호 1 57-66 (10 pages)
bacterial infection, therefore, intestinal epithelial cell death and shedding are critical events which eliminate instances of bacterial colonization and spreading by exfoliat- ing infected epithelial cells or readily replenishing neighbor- ing epithelial cells [79]. However, many bacterial pathogens are equipped with highly evolved infectious stratagems that manipulate intestinal epithelial cell...