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Streptococcus faecalis var. liquefaciens 전세포 고정화법을 이용한 Casein Phosphopeptides 생산
이기성, 신재윤, 장이현, 권대혁, 박기문, 진용수, Lee. Ki-Sung, Shin. Jae-Yoon, Jang. Yi-Hyun, Kweon. Dae-Hyuk, Park. Ki-Moon, Jin. Yong-Su 한국생물공학회 한국생물공학회지 6 Pages
한국생물공학회 한국생물공학회지 2008, Vol.23 No.1 59-64 (6 pages)
Streptococcus faecalis var. liquefaciens를 calcium alginate gel에 고정화 후 CPP생산 가능성을 실험하였다. 균체를 회수하여 담체에 고정화하는 방법보다는 배양액 전체를 고정화하는 방법이 보다 높은 생산성으로 CPP를 생산하였다. Streptococcus faecalis var. liquefaciens 전세포 고정화 방법에 의한 sodium casenate로부터의 CPP 생산의 최적조건은 bioreactor부피대비 bead사용량이 30%, 반응온도는 $50^{circ}C$, 반응 pH는 7.0, 기질의 농도는 10% 이었다. 또한, 고정화 균체를 이용한 연속적인 생산은 회분식 반응에서... -
Streptococcus faecalis의 pAM $eta$$_1$을 lactobacillus casei로의 전이에 영향을 미치는 요인
허정원, 강성훈 한국미생물학회 미생물학회지 4 Pages
한국미생물학회 미생물학회지 1990, Vol.28 No.3 188-191 (4 pages)
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Streptococcus faecalis DS 5 Plasmid pAM $eta_1$의 Lactobacillus casei YIT 9018로의 전이
허정원, 김정호, 정기철, 이용규, 김창렬 한국미생물생명공학회 산업미생물학회지 5 Pages
한국미생물생명공학회 산업미생물학회지 1990, Vol.18 No.3 317-321 (5 pages)
사용되는 Lactobacillus casei YIT 9018에 광범위 숙주영역을 갖고 있는 Streptococcus faecalis DS5 의 plasmid pAM $eta_1$ DNA를 이용하여 전이, 도입하고자 유당발효능결손변이주를 이용하여 원형질체융합, 접합, 형질전환을 검토하였던 바 얻어진 결과는 다음과 같다. L.casei와 S.faecalis 균주의 conjugation 은 membrane filter mating(Milipore, 0.45$mu extrm m$, front side)의 경우 $6.9 imes 10^7$으로 가장 높은 전이율을 나타냈으며 protoplast fusion이나 transformation에 의해서는 fusant나 transformant를 선별할 수... -
Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods
I. U. Khan, A. A. Hassan, A. Abdulmawjood, C. Lammler, W. Wolter, M. Zschock 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 3 213-223 (11 pages)
by a great variety of strains. Key words: Streptococcus uberis, Streptococcus parauberis, 16S rDNA, 23S rDNA, 16S-23S rDNA intergenic spacer region, CAMP factor gene cfu, skc/pauA genes Introduction Streptococcus uberis is world wide known as an environmental pathogen responsible for a high proportion of cases of clinical, mostly subclinical mastitis in lactating cows and is also the predominant... In the present study 130 S. uberis strains and one S. parauberis strain isolated from bovine milk samples of 58 different farms of various locations in Hesse, Germany, as well as two reference strains of each species were comparatively investigated for cultural, biochemical, serological and molecular properties. All S. uberis strains produced the enzyme β-D-glucuronidase, while the S. parauberis strains were negative. The S. uberis and S. parauberis 16S rRNA genes were amplified by polymerase... -
Streptococcus facalis var. liquefaciens에 존재하는 Plasmid DNA의 특성
강국희, 이명기, 박연희 한국미생물생명공학회 산업미생물학회지 6 Pages
한국미생물생명공학회 산업미생물학회지 1985, Vol.13 No.4 417-422 (6 pages)
Streptococcus faecalis var. liquefaciens에서 plasmid DNA를 분석한 결과, 4개의 plasmid를 가지고 있었으며, 각각의 대략적인 분자량은 6.8M-dal, 5.2Mdal, 2.6Mdal, 2.1Mdal로 측정되었다. 이 균주를 novobiocin으로 처리하여, 각각 다른 2개의 plasmid가 소실된 2개의 변이주를 얻었다. 이들 균주는 당발효성, 온도감수성, gelatin 용해성, 단백질응고성은 wild type과 동일하였으나 이중 한 균주가 lincomycin과 erythromycin에 대한 감수성을 나타내었다. 따라서 본 실험에서 검출한 4개의 plasmid는 당발효성 등의 특성과는... -
Distribution ofthe abortive agents detected from bovine abortion in Korea
Ji A Jung, Jong Wan Kim, Myeong ju Chae, Sang Ik Oh, Kyung Hyun Lee, Yeon Hee Kim, Byung Jae So, Ha Young Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 198 440-441 (2 pages)
(1/44, 2.3%), and congenital goiter (1/44, 2.3%). Isolation rate of bacteria from the placenta (6/15, 40%) was higher than those from fetal tissues (13/44, 29.5%); L. monocytogenes, E. coli, Streptococcus, Staphylococcus and so on. Among bacterial agents, L. monocytogenes was isolated from two (4.5%) of submitted fetus and one placenta at the same farm. And C. burnetii was detected from two... -
A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer
Qing Wang, Mianmian Chen, Wei Zhang 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 12 260-270 (11 pages)
B Streptococcus survival in vivo [28]. The DEGs enriched on pathway KO02010 related to ATP-binding cassette (ABC) transporters were upregulated under in vivo culture conditions. In bacteria, ABC transporters play a significant role in the uptake of essential nutrients, such as iron, heme, and vitamin B12, and are also important in the clearance of toxins and drugs [13,22]. Upregulated DEGs... Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction... -
Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene
Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 6 37-43 (7 pages)
et al. Bacillus anthracis [18], Streptococcus pneumonia [12], and Enterococcus faecalis [34]. Bacteriophage produced lytic enzymes that are active against staphylococcal cells have been previously reported [8,27]. A phage-associated lysin could rupture dead and living S. aureus cells [33]. In addition, phage lytic enzymes from staphylococcal phages 80a [3], Twort [14], 187 [13], phi11 [22],... Treatment of bovine mastitis caused by Staphylococcus (S.) aureus is becoming very difficult due to the emergence of multidrug-resistant strains. Hence, the search for novel therapeutic alternatives has become of great importance. Consequently, bacteriophages and their endolysins have been identified as potential therapeutic alternatives to antibiotic therapy against S. aureus. In the present study, the gene encoding lysin (LysSA4) in S. aureus phage SA4 was cloned and the nucleotide sequence... -
Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use
Hyeong-Jun Lim, So-Young Kim, Wan-Kyu Lee 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 16 391-395 (5 pages)
fermentation and cholesterol metabolism in cecectomized rats. Nutr Res 1999, 19, 235-245. 7.Fukushima M, Yamada A, Endo T, Nakano M. Effects of a mixture of organisms, Lactobacillus acidophilus or Streptococcus faecalis on delta6-desaturase activity in the livers of rats fed a fat- and cholesterol-enriched diet. Nutrition 1999, 15, 373-378. 8.Gallo LL. Cholesterol and other sterols: absorption,... in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase (BSH) activity assay by using MRS media added taurodeoxycholic acid (TDCA) and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies... -
젖소 유방염에서 16S rRNA 파이로시퀀싱을 이용한 우유 내 마이크로바이옴의 동정과 난백의 항균효과
김단일, 김은경, 성원진, 노영혜, 고대성, 김남형, 김재홍, 권혁준 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 10 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 2호 7 117-126 (10 pages)
(UC , 2.14%), Carnobacterium maltaromaticum (3.25%), Oceanisphaera profunda (3.45%) Serratia liquefaciens (6.56%), Enterococcus faecalis (0.4%), Staphylococcus chromogenes (0.06%), Staphylococcus epidermidis (0.03%), Lactococcus raffinolactis (0.06%) Streptococcus dysgalactiae ssp equisimilis, Enterococcus faecalis, Streptococcus dysgalactiae ssp dysgalactiae, Staphylococcus chromogenes PMM10...