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전체 선택해제
총 110건 검색
본문검색 결과만 보기
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Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD
Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 2 7-19 (13 pages)
sensitive detection techniques. For examples, immunomagnetic separation (IMS) techniques have been employed widely within routine microbiology testing laboratories for the isolation of specific microorganisms [9,20]. IMS allows the rapid capture and concentration of bacteria from a range matrics. The magnetic beads used for IMS are commercially available, either pre-coated with antibodies or ready... newly developed kit-E. coli O157:H7 Rapid kit. For the isolation of E. coli O157:H7, conventional culture, immunomagnetic separation, and E. coli O157:H7 Rapid kit were applied, and multiplex PCR and randomly amplified polymorphic DNA (RAPD) were performed for the molecular determination. There was high molecular relatedness among 11 Korean isolates and 17 U. S. strains at 63% level. Additionally, distinct differentiation between pig and cattle isolates was determined. It implied that RAPD had a... -
Isolation of Photobacterium damselae subsp. damselae from zebra shark Stegostoma fasciatum
Jee Eun Han , Dennis K. Gomez , Ji Hyung Kim , Casiano H. Choresca , Sang Phil Shin , Gun Wook Baeck , Se Chang Park 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 4 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2009, 제 49권 제 1호 5 35-38 (4 pages)
in the experimental infection study, the isolation of this pathogen in aquarium fish is significant because it can act as a reservoir to other aquatic animals and can also be zoonotic potential to human during aquarium management. This paper describes the first isolation of P. damselae subsp. damselae from zebra shark. Keywords :aquarium, Photobacterium damselae subsp. damselae, zebra shark... -
Isolation and Characterization of Endosome Subpopulation in Chinese Hamster Ovarian Cells
Suh, Duk-Joon, Park, Mi-Yeon, Jung, Dong-Keun, Bae, Hae-Rahn 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1996, 제 30권 제 2호 2 197-208 (12 pages)
Kor J Physiol (1996) 30(2), 197-208 Isolation and Characterization of Endosome Subpopulation in Chinese Hamster Ovarian Cells Duk-Joon Suh, Mi-Yeon Park, Dong-Keun Jung and Hae-Rahn Bae Department of Physiology, Dong-A University College of Medicine, Pusan 602-103, Korea =ABSTRACT= Endosomes lower their internal pH by an ATP-driven proton pump, which is critical to dissociation of many... Endosomes lower their internal pH by an ATP-driven proton pump, which is critical to dissociation of many receptor-ligand complexes, the first step in the intracellular sorting of internalized receptors and ligands. Endosomes are known to exhibit n great range of pH values that can vary between 5.0 and 7.0 within a single cell although the factors that regulate endosomal pH remain uncertain. To evaluate the morphological and topological differences of endosomes in the different stages, confocal... -
Isolation and Characterization of a Bacteriophage Preying an Antifungal Bacterium
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 13 584-588 (5 pages)
oC. The super- Note Open Access Isolation and Characterization of a Bacteriophage Preying an Antifungal Bacterium Aryan Rahimi-Midani 1, Kyoung-Ho Kim 1, Seon-Woo Lee 2, Sang Bong Jung 3, and Tae-Jin Choi 1* 1Department of Microbiology, Pukyong National University, Busan 48547, Korea 2Department of Applied Biology, Dong-A University, Busan 49315, Korea 3Department of Clinical Laboratory Science,... Several Bacillus species were isolated from rice field soils, and 16S rRNA gene sequence analysis showed that Bacillus cereus was the most abundant. A strain named BC1 showed antifungal activity against Rhizoctonia solani. Bacteriophages infecting strain BC1 were isolated from the same soil sample. The isolated phage PK16 had an icosahedral head of 100 ± 5 nm and tail of 200 ± 5 nm, indicating that it belonged to the family Myoviridae. Analysis of the complete linear dsDNA... -
Isolation and Characterization of Rice OsHRL Gene Related to Bacterial Blight Resistance
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 17 417-420 (4 pages)
417-420 (2010) DOI: 10.5423/PPJ.2010.26.4.417 The Plant Pathology Journal The Korean Society of Plant Pathology Isolation and Characterization of Rice OsHRL Gene Related to Bacterial Blight Resistance Sang Ryeol Park 1*, Seok Jun Moon 1, Dongjin Shin 1, Min Gab Kim 1, Duk-Ju Hwang 1, Shin-Chul Bae 1, Jeong-Gu Kim 2, Bu Young Yi 3 and Myung Ok Byun 1 1Bio-crop Development Division and 2Genomics... The expression of HR-like lesion inducing gene of Oryza sativa (OsHRL) was slightly increased by Xanthomonas oryzae pv. oryzae (Xoo) infection. Transgenic rice plants over-expressing OsHRL gene were challenged with Xoo and the development of disease symptoms were examined to investigate the effect of OsHRL gene expression on plant defense responses. The over-expression of OsHRL increased disease resistance against Xoo compared with wild type plants. -
Characterization of Escherichia coli isolated from Korean native cattle
Yu Ran Lee, Ara Cho, Jong Wan Kim, Myeong ju Chae, Sang Ik Oh, Jae Won Byun, Byung Jae So, Dong mi Kwak, Ha Young Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 203 443-444 (2 pages)
or super market. Isolation of Sallmonellawas isolated from fecal swabs and total organs of turtles and environmentalsamples including water and feed. Salmonella was identified using multiplex PCR(multiplex polymerase chain reaction) method and VITEKⅡ bacterial identificationsystem. Serotypes and minimum inhibitory concentrat ions (MICs) of isolates weredetermined following conventional... -
Distribution of serovarsof Salmonella in pet turtles beingsold in South Korea
Byung Kook Choi, Ok Mi Jeong, Chun Tae Lim, So Youn Youn, Na Young Kim, Byung Woo Jeon, Shin Ae Kim, Suk Chan Jung , Min Su Kang 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 202 443-443 (1 pages)
or super market. Isolation of Sallmonellawas isolated from fecal swabs and total organs of turtles and environmentalsamples including water and feed. Salmonella was identified using multiplex PCR(multiplex polymerase chain reaction) method and VITEKⅡ bacterial identificationsystem. Serotypes and minimum inhibitory concentrat ions (MICs) of isolates weredetermined following conventional... -
Comparison of antibiotic and sanitizerresistance of Salmonella isolatesfrom chicken slaughter processes in Korea
So Youn Youn, Ok Mi Jeong, Byung Kook Choi, Suk Chan Jung, Min Su Kang 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 201 442-443 (2 pages)
or super market. Isolation of Sallmonellawas isolated from fecal swabs and total organs of turtles and environmentalsamples including water and feed. Salmonella was identified using multiplex PCR(multiplex polymerase chain reaction) method and VITEKⅡ bacterial identificationsystem. Serotypes and minimum inhibitory concentrat ions (MICs) of isolates weredetermined following conventional... -
Complete Genome of A Novel Phage KBNP 1315 that Infects Avian Pathogenic Escherichia coli and Functional Characterization of Its Lysis-cassette
Jae Wook Jung, Tae Hun Kim, Jung Seok Lee, Seong Bin Park, Seong Won Nho, Ki Sei Kim, Tae Hwan Kim, Jong Earn Yu, Se Pyeong Im, Si Won K 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 135 208-209 (2 pages)
and its bacteriophage: Bacterial strain, Escherichia coli 1315 for phage isolation was kindly provided by KBNP Technology Institute, KBNP, Inc. 2) Transmission electron microscopy (TEM): A solution (8 μl) of purified bacteriophage stained with 8 μl of 1% aqueous uranyl acetate (Merck, Darmstadt, Germany) was examined with Philips TECNAI F12 FEI transmission electron microscope (FEI,... -
Evidence of Intercontinental Transfer of Avian Influenza Viruses Carrying Nonstructural Allele B Gene Belonged to North American Lineage into Republic of Korea
Sun Ha Cheon, Yu Na Lee, Eun Kyeong Lee, Gyeong Beom Heo, Youn Jeong Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 110 265-266 (2 pages)
continuously. Materials and Methods: A total of 52,693 specimens by fresh fecal samples from migratory bird habitats in Korea from January 2012 to April 2017 were tested for this study. Virus isolation was attempted in embryonated chicken eggs for real time RT-PCR-positive samples. The viral RNA was extracted from allantoic fluid and RT-PCR was performed to determine full length of viral...