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Comparative studies on pheno- and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany
Siti Isrina Oktavia Salasia, Zaini Khusnan, Christoph Lammler, Michael Zschock 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 3 103-109 (7 pages)
..PAGE:7 Comparative studies on pheno- and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis 109 Staphylococcus aureus defective in fibronectin-binding proteins and studies on the expression of fnb genes. Mol Microbiol 1995, 17, 1143-1152. 12.Guidry A, Fattom A, Patel A, OBrien C. Prevalence of capsular serotypes among Staphylococcus aureus isolates from cows... In the present study, 35 Staphylococcal strain isolated from milk samples of 16 cows from eight farms of three different geographic locations in Central Java, Indonesia, and from milk samples of 19 cows from 19 farms of different geographic locations in Hesse, Germany, were compared pheno- and genotypically. On the basis of cultural and biochemical properties as well as by amplification of the 23S rRNA specific to Staphylococcus aureus, all isolates could be identified as S. aureus. In addition,... -
Identification of Leonurus sibiricus as a Weed Reservoir for Three Pepper-Infecting Viruses
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 1호 9 65-69 (5 pages)
and appropriate primers for TSWV described in the pre- vious studies (Lee et al., 2011; Yoon et al., 2005). The resulting PCR products were purified using QIAquick PCR purification kit (QIAGEN, Hilden, Germany) and cloned into pGEM-T easy Vector (Promega, Madison, USA) and sequenced using specific primers (Lee et al., 2011; Yoon et al., 2005). End sequences of each virus were obtained by the... In plant virus ecology, weeds are regarded as wild reservoirs of viruses and as potential sources for insect-mediated transmission of viruses. During field surveys in 2013–2014, three Leonurus sibiricus plants showing virus-like symptoms were collected from pepper fields in Daegu, Seosan, and Danyang in Korea. Molecular diagnosis assays showed that the collected L. sibiricus samples were infected with either Tomato spotted wilt virus (TSWV), Pepper mild mottle virus (PMMoV), or Beet... -
Improvement Characteristics of Bio-active Materials Coated Fabric on Rat Muscular Mitochondria
DongheeLee, Young-WonKim, Jung-HaKim, MisukYang, HyemiBae, InjaLim, HyoweonBang, Kyung-ChanGo, Gwang-WungYang, Yong-HwanRho, Hyo-SukPark, E 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.3 14 283-289 (7 pages)
(15 ng) and up to 10 μl of distilled water (DW). Real-time PCR was performed in a LightCycler 2.0 (Roche). The real-time PCR conditions were as follows: de- naturation (95 o C for 10 min), a three-segment amplification program repeated 35 times (95 oC for 10 sec; 62 oC for 10 sec; 72 o C for 10 sec), melting curve program (65 o C to 95 o C with a heating rate of 0.1 o C/sec) and cooling down... This study surveys the improvement characteristics in old-aged muscular mitochondria by bio-active materials coated fabric (BMCF). To observe the effects, the fabric (10 and 30%) was worn to old-aged rat then the oxygen consumption efficiency and copy numbers of mitochondria, and mRNA expression of apoptosis- and mitophagy-related genes were verified. By wearing the BMCF, the oxidative respiration significantly increased when using the 30% materials coated fabric. The mitochondrial DNA copy... -
The reliability of dual rehabilitative ultrasound imaging measurements for muscle co-activation
Joo-hee Hahn, Hyun-Geun Ha, Hwang-Jae Lee, Seung-yeop Lim, Wan-hee Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 6 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2017, 제 6권 제 3호 8 152-157 (6 pages)
co-activation state. The D-RUSI is the only instrument that can assess and measure two muscles at the same time with two probes; therefore, the results cannot be directly compared with previous studies. In this study, the D-RUSI of the BB muscle had very good ..PAGE:4 Hahn, et al: The reliability of dual rehabilitative ultrasound imaging 155 Table 2. Intra-rater reliability between repeated... Objective: The aim of this study was to determine the intra-rater and inter-rater reliability of the dual rehabilitative ultrasound imaging (D-RUSI) when simultaneously measuring muscle thickness changes at rest and during co-contraction of the biceps brachii (BB) and triceps brachii (TB). Design: Cross-sectional study. Methods: This study included 36 healthy participants (23 men, 13 women). The participants sat on a chair in a comfortable position with a cushion placed under their elbow to... -
Development ofmultiplex RT-PCR assays to detect swine influenza virus (SIV) using the lineage-specificmarkers
Eun Jung Lee, Eun Ju Kim, Bo Hye Kim, Jae Young Song, In Soo Cho, Yeun Kyung Shin 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 126 398-398 (1 pages)
of Veterinary Science animals. More studies should be performed to estimate the prevalence ofSFTSV in domestic/wild animals in the RO Korea. References [1] NiuG, Li J, Liang M, Jiang X, Jiang M, Yin H, Wang Z, Li C, Zhang Q, Jin C, WangX, Ding S, Xing Z, Wang S, Bi Z, Li D. Severe Fever withThrombocytopenia Syndrome Virus among Domesticated Animals, China. Emerg InfectDis. 2013 May;19(5):... -
Comparison of electrophysiological properties of two types of pre-sympathetic neurons intermingled in the hypothalamic paraventricular nucleus
Yiming Shen, Seong Kyu Han, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 2 483-491 (9 pages)
63 receptors (rise time, ∼20 msec) in neuron-human embryonic kidney cell heterosynapses [37]. Further studies are needed to fully elucidate the detailed mechanisms underlying the differences observed in this study in the intrinsic and synaptic properties of these two neuron groups. Overall, our results suggest that the lower firing rate seen in the PVN RVLM neurons may have been due to the... The hypothalamic paraventricular nucleus (PVN) contains two types of neurons projecting to either the rostral ventrolateral medulla (PVNRVLM) or the intermediolateral horn (IML) of the spinal cord (PVNIML). These two neuron groups are intermingled in the same subdivisions of the PVN and differentially regulate sympathetic outflow. However, electrophysiological evidence supporting such functional differences is largely lacking. Herein, we compared the electrophysiological properties of these... -
Comparison of the effects of isoflurane versus propofolremifentanil anesthesia on oxygen delivery during thoracoscopic lung lobectomy with one-lung ventilation in dogs
Jiyoung Park, Hae-Beom Lee, Seong Mok Jeong 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 12 426-433 (8 pages)
vivo study [5,10,32]. Moreover, some studies excluded surgical maneuver and evaluated only under 1LV [3,14,17]. Consequently, it is inappropriate to compare results among studies, thus effects under real surgical condition remain unpredictable in dogs. Considering these aspects, this study investigated the effects of two anesthetic regimens, isoflurane inhalation and propofol- remifentanil... This study compared effects of isoflurane inhalation (ISO) and propofol-remifentanil combined total intravenous anesthesia (TIVA) on oxygenation during thoracoscopic lung lobectomy with 30-min one-lung ventilation (1LV). Thoracoscopic right middle lung lobectomy was performed in ten dogs divided into ISO and TIVA groups, and cardiopulmonary parameters were measured with blood gas analysis. Throughout the study, isoflurane was inhaled up to 1.5%, and the infusion rates of propofol and... -
A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer
Qing Wang, Mianmian Chen, Wei Zhang 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 12 260-270 (11 pages)
to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682... Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction... -
A serological study of severe fever with thrombocytopenia syndrome using a virus neutralization test and competitive enzyme-linked immunosorbent assay
Hyojin Lee, Eun-Ju Kim, In-Soo Cho, Jae-Young Song, Jeong Soo Choi, Ji Youn Lee, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 5 33-38 (6 pages)
VN results was higher than that (84.3%) between the cELISA and IFA. These findings indicate that our newly developed cELISA can be used for surveillance or epidemiological studies of SFTSV in animals. Keywords: competitive enzyme-linked immunosorbent assay, severe fever with thrombocytopenia syndrome, virus neutralization test Introduction Severe fever with thrombocytopenia syndrome (SFTS) is a... samples (including two bovine positive control sera) and 70 horse serum samples. The consistency (98.9%) between cELISA and VN results was higher than that (97.4%) between cELISA and IFA for the bovine serum samples. Similarly, for the horse serum samples, the consistency (88.6%) between cELISA and VN results was higher than that (84.3%) between the cELISA and IFA. These findings indicate that our newly developed cELISA can be used for surveillance or epidemiological studies of SFTSV in animals. -
Deep anterior lamellar keratoplasty of dog eyes using the big-bubble technique
Soohyun Kim, Ji Yoon Kwak, Manbok Jeong, Kangmoon Seo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 11 347-352 (6 pages)
and frequent graft rejections characterized by corneal vascularization, graft failure, and subsequent corneal edema. Therefore, few studies have investigated canine corneal transplantation [20]. Deep anterior lamellar keratoplasty (DALK) removes and replaces the pathologic corneal stroma while preserving the host endothelium, which eliminates the risk of endothelial graft rejection and has... This study was conducted to establish the feasibility of corneal transplantation using the big-bubble technique (BBT) to perform deep anterior lamellar keratoplasty (DALK) in three dogs. After the cornea was trephined 750 ?m, 4 mL of air was injected, and the blanched stroma was removed to expose Descemet’s membrane (DM). The donor corneal button, which was gently stripped off the DM, was sutured onto the bare DM of the recipient cornea. The dogs received topical antibiotics every 6 h for 7...