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An angiotensin II type 1 receptor blocker attenuates TRAIL resistance in human lung cancer via inhibition of autophagy flux
Mohammad Rasheduzzaman, Jeong Min Hong, Uddin Nazim, Hong hua Yin, You Jin Lee, Jae Won Seol, Sang Youel Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 235 338-339 (2 pages)
To maintain undifferentiated state of iPSCs in vitro, cells have been traditio nallymaintained on mouse embryonic fibroblast (MEF) feeders and passaged by enzymaticor mechanical dissociation methods. Enzymatic dissociation method istime-critical. Over-treatment of cells with detachment solution often resultsin increased production of single cells. Mechanical dissociation by scrapingselected... -
Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood
Hyo-Jong Lee, Kyung-Sun Kang, Sun-Young Kang, Hyung-Sik Kim, Se-Jin Park, Seung-Yong Lee, Kwang-Dong Kim, Hee-Chun Lee, Ji-Kwon 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 4 289-297 (9 pages)
of type II collagen, one of the chondrocyte specific markers. Furthermore, chondrocytes were stained positively with Safranin-O dye and immunofluorescence stain of type II collagen protein. Immunophenotypes of undifferentiated and differentiated MSC were determined by flow cytometric analysis and immunocyometric staining. Evaluation of the HLA-ABC antigen revealed moderate expression for... and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogene... -
Topology of Scavenger Receptor Class B Type I (SR-BI) on Brush Border Membrane
Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 3 265-272 (8 pages)
the differentcell differentiation status (Figure 6A). Undifferentiated Caco-2 cells showed band only at 57 kDa (Figure 6A; lanes 1, 2), whereas differentiated cells showed several different sizes (>57 kDa) of bands (Figure 6A; lanes 3, 4). The increasing sizes of SR-BI derivatives might be resulted from the maturation of protein (e.g. glycosylation and dimerization). 125I apo A-I overlay analysis... Both hydropathy plot and in vitro translation results predict the topology of SR-BI; the receptor is an integral membrane protein of 509 amino acids, consisting of a short cytoplasmic N-terminus of 9 amino acids followed by a first transmembrane domain of 22 amino acids, the extracellular domain of 408 amino acids, the second transmembrane domain of 22 amino acids, and the cytoplasmic C-terminus of 47 amino acids. The immunoblot of rBBMV in the presence or absence of pAb589 peptide antigen (the... -
Involvement of NMDA Receptor and L-type Calcium Channel in the Excitatory Action of Morphine
BonSeopKoo, HongKeeShin, SukHanKang, JongHunJun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.5 2 241-246 (6 pages)
nifedipine, verapamil and pertussis toxin, suggesting that both extracellular Ca 2+ and Ca 2+ released from IP3- sensitive stores play an important role in the increase in [Ca 2+ ]i. However, in undifferentiated NG 108-15 cells, a δ- opioid agonist dose-dependently increased [Ca 2+ ]i only through the mechanism of IP 3 formation which was blocked by thapsigargin and pertussis toxin, but not... We studied the excitatory action of morphine on the responses of dorsal horn neuron to iontophoretic application of excitatory amino acid and C-fiber stimulation by using the in vivo electrophysiological technique in the rat. In 137 of the 232 wide dynamic range (WDR) neurons tested, iontophoretic application of morphine enhanced the WDR neuron responses to N-methyl-D-aspartate (NMDA), kainate, and graded electrical stimulation of C-fibers. Morphine did not have any excitatory effects on the... -
Repair of ischemic injury by pluripotent stem cell derived endothelial cells without teratoma formation through selective photosensitivity
Seung Ju Cho, So Yeon Kim, Ho Chang Jeong, Hyung Min Chung, Sung Hwan Moon, Hyuk Jin Cha 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 11 83-83 (1 pages)
pluripotent stem cells (PSCs) is one of serious concern in PSC-based therapies. Small molecules toxic to PSCs, which can selectively deplete undifferentiated PSCs, have been developed to reduce the risk of teratoma formation. However, despite their high efficacies, such chemical-based approaches may carry unexpected toxicities on specific differentiated cell types. We aimed to develop a novel... -
Exploration of the developmental process of vasculogenic mimicry incanine inflammatory mammary carcinoma: compared with comedocarcinoma
Ji Su Choi, Ye In Oh, Byung Woo Lee, Hyun Wook Kim, Byung Il Yoon 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 277 360-360 (1 pages)
and immunohistochemical stains for cytokeratin,vimentin, desmin, and s-100 were performed. Ki-67 index was evaluated in the tumors. Results: Histopathologically, the primary tumor showed undifferentiated pleomorphic spindle cell sarcoma (SCS) with storiform and solidpatterns, poor encapsulation, invading into adjacent tissue. Immunohistochemical tests showed positive immunoreactivity for... -
Establishment of live tissue bank of a soft tissue sarcoma from the hind limb of a Maltese female dog
Sang Hwa Ryu, Ye In Oh, Byung Woo Lee, Hyun Wook Kim, Byung Il Yoon 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 276 359-360 (2 pages)
and immunohistochemical stains for cytokeratin,vimentin, desmin, and s-100 were performed. Ki-67 index was evaluated in the tumors. Results: Histopathologically, the primary tumor showed undifferentiated pleomorphic spindle cell sarcoma (SCS) with storiform and solidpatterns, poor encapsulation, invading into adjacent tissue. Immunohistochemical tests showed positive immunoreactivity for... -
Comparison of Dissociation Methods on Pluripotency of Stem Cells
Na Yeon Gu, Mi Jeong Park, Jeong Su Byeon, Da Un Jeong, Jienny Lee, In Soo Cho, Sang Ho Cha 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 234 338-338 (1 pages)
To maintain undifferentiated state of iPSCs in vitro, cells have been traditio nallymaintained on mouse embryonic fibroblast (MEF) feeders and passaged by enzymaticor mechanical dissociation methods. Enzymatic dissociation method istime-critical. Over-treatment of cells with detachment solution often resultsin increased production of single cells. Mechanical dissociation by scrapingselected... -
A Salmonella Typhi ghost induced by the E gene of phage X174 stimulates dendritic cells and efficiently activates the adaptive immune response
Gayeon Won, Seong Kug Eo, Sang-Youel Park, Jin Hur, John Hwa Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 9 536-542 (7 pages)
by DCs to undifferentiated T-cell populations [28]. The initiation of the adaptive immune response by a host against the invasion of S Typhi depends on the antigen processing capacity of DCs and the resulting level of T-cell activation [27]. To activate naive T-cell subsets, exogenous or endogenous antigens initially need to be recognized and processed by dendritic cells. Antigen-derived... Previously, we genetically engineered a Salmonella Typhi bacterial ghost (STG) as a novel inactivated vaccine candidate against typhoid fever. The underlying mechanism employed by the ghost in stimulating the adaptive immune response remains to be investigated. In this study, we aimed to evaluate the immunostimulatory effect of STG on mouse bone marrow-derived dendritic cells (BMDCs) and its activation of the adaptive immune response in vitro. Immature BMDCs were stimulated with STG, which... -
Comparison of the characteristics of canine adipose tissue-derived mesenchymal stem cells extracted from different sites and at different passage numbers
Kevin M. Yaneselli, Cristiana P. Kuhl, Paula B. Terraciano, Fernanda S. de Oliveira, Sabrina B. Pizzato, Kamila Pazza, Alessandr 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 3 13-20 (8 pages)
is less invasive [11] and AD-MSCs conserve their undifferentiated state during more passages [15]. These characteristics have motivated researchers to focus on AT as a safe and convenient source of MSCs for use in regenerative medicine [9,11]. Several studies in humans and animal models have compared the characteristics of AD-MSCs derived from different anatomical sites, such as those in... Mesenchymal stem cells (MSCs) have desirable characteristics for use in therapy in animal models and veterinary medicine, due to their capacity of inducing tissue regeneration and immunomodulation. The objective of this study was to evaluate the differences between canine adipose tissue-derived MSCs (AD-MSCs) extracted from subcutaneous (Sc) and visceral (Vs) sites. Surface antigenic markers, in vitro differentiation, and mineralized matrix quantification of AD-MSCs at different passages (P4,...