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Real-Time Vehicle License Plate Detection Based on Background Subtraction and Cascade of Boosted Classifiers
Sarker. Md. Mostafa Kamal, Song. Moon Kyou 한국통신학회 한국통신학회논문지. The Journal of Korea Information and Communications Society. 통신이론 및 시스템 11 Pages
한국통신학회 한국통신학회논문지. The Journal of Korea Information and Communications Society. 통신이론 및 시스템 2014, No.0 909-919 (11 pages)
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Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing
Hyeon-Joong Kim, Joon Young Kim, Byung-Hwan Lee, Sun-Hye Choi, Hyewon Rhim, Hyoung-Chun Kim, Seoung-Yob Ahn, Soon-Wuk Jeong, Min 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 16 387-397 (11 pages)
In Situ Apoptosis Detection Kit (S7100; Millipore) was used to analyze the paraffin sections [14]. To assess the degree of cell proliferation in corneal wound healing, immunohistochemical analysis for proliferating cell nuclear antigen (PCNA) was performed on the paraffin sections by using mouse anti-PCNA antibody (1:2,000; Santa Cruz Biotechnology, USA) as previously described [3]. The H&E... Ginseng gintonin is an exogenous ligand of lysophosphatidic acid (LPA) receptors. Accumulating evidence shows LPA helps in rapid recovery of corneal damage. The aim of this study was to evaluate the therapeutic efficacy of gintonin in a rabbit model of corneal damage. We investigated the signal transduction pathway of gintonin in human corneal epithelium (HCE) cells to elucidate the underlying molecular mechanism. We next evaluated the therapeutic effects of gintonin, using a rabbit model of... -
Anti-adipogenic effect of Artemisia annua in diet-induced-obesity mice model
Hye Kyung Baek, Hyeji Shim, Hyunmook Lim, Minju Shim, Chul-Kyu Kim, Sang-Kyu Park, Yong Seok Lee, Ki-Duk Song, Sung-Jo Kim, Sun 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 1 389-396 (8 pages)
to be surrounded by immune related cells in obese animals and humans [3]. It is also well known that measurements of fat accumulation are directly proportional to adiposity [10]. Therefore, overt detection of macrophages indicates excessive adipogenesis. Macrophages, which secrete many types of adipokines and cytokines, are related to the innate inflammation response [24]. Continuous adipose... Obesity has increased continuously in western countries during the last several decades and recently become a problem in developing countries. Currently, anti-obesity drugs originating from natural products are being investigated for their potential to overcome adverse effects associated with chemical drugs. Artemisinic acid, which was isolated from the well-known anti-malaria herb Artemisia annua (AA) L., was recently shown to possess anti-adipogenic effects in vitro. However, the... -
Oxidative response of neutrophils to platelet-activating factor is altered during acute ruminal acidosis induced by oligofructose in heifers
Claudia Concha, María Daniella Carretta, Pablo Alarcón, Ivan Conejeros, Diego Gallardo, Alejandra Isabel H 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 7 217-224 (8 pages)
until the end of the HPLC run after 40 min. Lactic acid detection was performed at 210 nm, and the temperature of the column oven was 25 o C. Lactic acid recovery was estimated at 94%, and the detection limit was estimated to be 0.05 mM. In vitro ROS production in bovine neutrophils during acute ruminal acidosis To assess the effects of ruminal acidosis on neutrophil respiratory burst,... Reactive oxygen species (ROS) production is one of the main mechanisms used to kill microbes during innate immune response. D-lactic acid, which is augmented during acute ruminal acidosis, reduces platelet activating factor (PAF)-induced ROS production and L-selectin shedding in bovine neutrophils in vitro. This study was conducted to investigate whether acute ruminal acidosis induced by acute oligofructose overload in heifers interferes with ROS production and L-selectin shedding in blood... -
Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice
Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 5 199-208 (10 pages)
days after the final immunization, mice were sacrificed and splenocytes were harvested. Detection of antibodies to PRRSV using indirect ELISA (iELISA) PRRSV-specific IgG and sIgA were examined as follows: 96-well ELISA plates were coated with purified-PRRSV antigen at a concentration of 5 μg/mL at 4 o C overnight, then blocked with PBST (PBS containing 0.5% Tween-20) containing 5% skimmed... the development of novel PRRSV vaccines is urgently needed. Previous studies have suggested that yeast possesses adjuvant activities, and it has been used as an expression vehicle to elicit immune responses to foreign antigens. In this report, recombinant Kluyveromyces lactis expressing GP5 of HP-PRRSV (Yeast-GP5) was generated and immune responses to this construct were analyzed in mice. Intestinal mucosal PRRSV-specific sIgA antibody and higher levels of IFN-γ in spleen CD4 and CD8 T cells... -
Lysophosphatidic acid enhances breast cancer cells-mediated osteoclastogenesis
Ju-Suk Nam, Ashish Ranjan Sharma, Lich Thi Nguyen, Supriya Jagga, Yeon-Hee Lee, Garima Sharma, Sang-Soo Lee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 4 503-511 (9 pages)
the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright Korean J Physiol Pharmacol, pISSN 1226-4512, eISSN 2093-3827 INTRODUCTION Breast cancer is the most common malignant tumor diagnosed among women worldwide. Despite continued improvement in... Lysophosphatidic acid (LPA) is known to play a critical role in breast cancer metastasis to bone. In this study, we tried to investigate any role of LPA in the regulation of osteoclastogenic cytokines from breast cancer cells and the possibility of these secretory factors in affecting osteoclastogenesis. Effect of secreted cytokines on osteoclastogenesis was analyzed by treating conditioned media from LPA-stimulated breast cancer cells to differentiating osteoclasts. Result demonstrated that... -
Histone deacetylase inhibition attenuates hepatic steatosis in rats with experimental Cushing’s syndrome
MinaKim, Hae-AhmLee, Hyun-MinCho, Seol-HeeKang, EunjoLee, InKyeomKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.1 3 23-33 (11 pages)
Ko- rea). Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) RT-qPCR was performed using an ABI PRISM 7000 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) as previously described [21]. Amplification reactions containing 10 μL of SYBR Green PCR Master Mix (Applied Biosystems), 4 μL of cDNA, and the indicated primer set (200 nM) were performed in a... Cushing’s syndrome (CS) is a collection of symptoms caused by prolonged exposure to excess cortisol. Chronically elevated glucocorticoid (GC) levels contribute to hepatic steatosis. We hypothesized that histone deacetylase inhibitors (HDACi) could attenuate hepatic steatosis through glucocorticoid receptor (GR) acetylation in experimental CS. To induce CS, we administered adrenocorticotropic hormone (ACTH; 40 ng/kg/day) to Sprague-Dawley rats by subcutaneous infusion with osmotic mini-pumps. We... -
Preventive effects of imperatorin on perfluorohexanesulfonate-induced neuronal apoptosis via inhibition of intracellular calcium-mediated ERK pathway
EunkyungLee, So-YoungChoi, Jae-HoYang, YounJuLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.4 9 399-406 (8 pages)
96 V luminometer (Berthold Detection System, Oak Ridge, TN, USA). The data were represented as fold increase over the control. TUNEL staining DNA fragmentation was detected with terminal deoxynu cleotidyl transferase (TdT)mediated dUTPnick endlabeling (TUNEL) assay kit (DeadEnd TM fluorometric TUNEL system, Promega) according to the manufacturer’s protocol. In brief, cells grown on... Early life neuronal exposure to environmental toxicants has been suggested to be an important etiology of neurodegenerative disease development. Perfluorohexanesulfonate (PFHxS), one of the major perfluoroalkyl compounds, is widely distributed environmental contaminants. We have reported that PFHxS induces neuronal apoptosis via ERK-mediated pathway. Imperatorin is a furanocoumarin found in various edible plants and has a wide range of pharmacological effects including neuroprotection. In this... -
4-(Tert-butyl)-2,6-bis(1-phenylethyl)phenol induces pro-apoptotic activity
JunHoKim, YunmiLee, Mi-YeonKim, JaeYoulCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.3 4 253-259 (7 pages)
V Apoptosis Detection Kit I that detects phosphatidylserine-based changes in cell membranes [13,14]. Cells were plated in 12-well culture plate at a seeding density of 4×10 5 cells/dish (5×10 5 cells/ml), and KTH-13-t-Bu was added to the culture medium to the specified concentration. Vehicle alone was used as the untreated control. The subsequent procedures were conducted according to the... Previously, we found that KTH-13 isolated from the butanol fraction of Cordyceps bassiana (Cb-BF) displayed anti-cancer activity. To improve its anti-proliferative activity and production yield, we employed a total synthetic approach and derivatized KTH-13 to obtain chemical analogs. In this study, one KTH-13 derivative, 4-(tert-butyl)-2,6-bis(1-phenylethyl)phenol (KTH-13-t-Bu), was selected to test its anti-cancer activity. KTH-13-t-Bu diminished the proliferation of C6 glioma, MDA-MB-231,... -
Identification of Lys49-PLA2 from crude venom of Crotalus atrox as a human neutrophil-calcium modulating protein
Md.TipuSultan, Hong-MeiLi, YongZuLee, SoonSungLim, Dong-KeunSong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.2 6 177-183 (7 pages)
staining was >95%. Eosinophils were found to be <5%. The viability of neutrophils stained with tryphan blue was >99%. Lactate dehydrogenase (LDH) activity assay Cytotoxicity was evaluated by the detection of LDH activity with cytotoxicity detection kit (Takara) according to the manu- facturer’s protocol as follows. Briefly, neutrophils were incubated with different concentrations of K49-PLA2... We fortuitously observed a human neutrophil intracellular free-calcium concentration ([Ca2+]i) increasing activity in the commercially available phosphodiesterase I (PDE I), which is actually dried crude venom of Crotalus atrox. As this activity was not observed with another commercially available pure PDE I, we tried to find out the causative molecule(s) present in ‘crude’ PDE, and identified Lys49- phospholipase A2 (Lys49-PLA2 or K49-PLA2), a catalytically inactive protein which belongs to...