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Decreased Voltage Dependent K+ Currents in Cerebral Arterial Smooth Muscle Cells of One-Kidney, One-Clip Goldblatt Hypertensive Rat
YoungSunOh, SeHoonKim, HoeSukKim, ByeongHwaJeon, SeokJongChang, KwangJinKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.5 3 471-479 (9 pages)
to zero current level even at high membrane potentials for 20 40 s prepulse. The plotted data from two groups were fit with Boltzmann equation. There was no significant difference in values of half inactivation voltage (V 1/2) between normotensive rats and lK，IC-GBH rats， which were -53.9:2.5 and -45.8:2.8 mV respec tively. πle values of slope factor (k1) between normo tensive rats and... Kv current was smaller in 1K,1C-GBH rat than in normotensive rat. The voltage dependence of steady-state activation and inactivation of Kv channel determined by using double-pulse protocol showed no significant difference. These results suggest that 4-AP-sensitive Kv channels play a major role in the regulation of membrane potential in cerebral arterial smooth muscle cells and alterations of 4-AP- sensitive KV channels would contribute to hypopolarization of membrane potential in 1K,1C-GBH rat. -
Neural circuit remodeling and structural plasticity in the cortex during chronic pain
WoojinKim, SunKwangKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.1 1 1-8 (8 pages)
nucleus of mice after transection of the peripheral whisker sensory nerve, using the whole-cell voltage-clamp technique [26]. Also, Draganski et al. have reported a loss of thalamic gray matter in 28 volunteers with unilateral limb amputation using voxel-based morphometry, which is based on high-resolution magnetic resonance images [27]. They suggested that the loss of neurons might be a... Damage in the periphery or spinal cord induces maladaptive plastic changes along the somatosensory nervous system from the periphery to the cortex, often leading to chronic pain. Although the role of neural circuit remodeling and structural synaptic plasticity in the ‘pain matrix’ cortices in chronic pain has been thought as a secondary epiphenomenon to altered nociceptive signaling in the spinal cord, progress in whole brain imaging studies on human patients and animal models has suggested a... -
Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber
Isabelle Ruhnke*, John V. DeBiasio, Jan S. Suchodolski, Shelley Newman, Mark W. Musch, Jörg M. Steiner 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 17 297-307 (11 pages)
sodium gradients. Therefore, the decrease in the sodium gradient of the enterocyte in response to ouabain would be a secondary mechanism decreasing basolateral Cl entry. The equivocal effect of ouabain on the short circuit current has also been observed in previous studies and shown to be correlated with the preparation of tissue sample [29]. If the mucosa was still attached to the plexus... This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from... -
Enhancement of GluN2B Subunit-Containing NMDA Receptor Underlies Serotonergic Regulation of Long-Term Potentiation after Critical Period in the Rat Visual Cortex
KayoungJoo, Duck-JooRhie, , Hyun-JongJang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.6 7 523-531 (9 pages)
in the decrease in synaptic plasticity. This assumption could be supported by the stud- ies demonstrating that manipulations for enhancing plasti- city in aged animals are acco mpanied with the changes in GABA AR-mediated inhibition, NMDAR properties, and ex- tracellular matrix [9-11]. These studies on methods to en- hance plasticity in aged animals have helped to understand the underlying... Serotonin [5-hydroxytryptamine (5-HT)] regulates synaptic plasticity in the visual cortex. Although the effects of 5-HT on plasticity showed huge diversity depending on the ages of animals and species, it has been unclear how 5-HT can show such diverse effects. In the rat visual cortex, 5-HT suppressed long-term potentiation (LTP) at 5 weeks but enhanced LTP at 8 weeks. We speculated that this difference may originate from differential regulation of neurotransmission by 5-HT between the age... -
Effects of Serotonin on the Induction of Long-term Depression in the Rat Visual Cortex
Hyun-JongJang, Kwang-HyunCho, , Sung-WonPark, Myung-JunKim, ShinHeeYoon, Duck-JooRhie 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.5 14 337-343 (7 pages)
a developmental decrease in plasticity, we investigated the effect of 5-HT on the induction of LTD in the pathway from layer 4 to layer 2/3. LTD was inhibited by 5-HT (10 μM) in 5-week-old rats. The inhibitory effect was mediated by activation of 5-HT 2 receptors. Since 5-HT also regulates the development of visual cortical circuits, we also investigated the role of 5-HT on the development of... a developmental decrease in plasticity, we investigated the effect of 5-HT on the induction of LTD in the pathway from layer 4 to layer 2/3. LTD was inhibited by 5-HT (10ՌM) in 5-week-old rats. The inhibitory effect was mediated by activation of 5-HT2 receptors. Since 5-HT also regulates the development of visual cortical circuits, we also investigated the role of 5-HT on the development of inhibition. The development of inhibition was retarded by chronic (2 weeks) depletion of endogenous... -
Differential Functional Expression of Clotrimazole-sensitive Ca2+-activated K+ Current in Bal-17 and WEHI-231 Murine B Lymphocytes
HaifengZheng, JaeHongKo, JooHyunNam, YungEEarm, SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2006, Vol.10 No.1 4 19-24 (6 pages)
(0.15 ml) was superfused at 5 ml/min, and voltage clamp experiments were performed at room temperature (22 ∼25 oC). Patch pipettes with a free-tip resistance of about 2.5 megohms were connected to the head stage of a patch-clamp amplifier (Axopatch-1D, Axon Instruments). Liquid junction potentials were corrected with an offset circuit before each experiment. Unless men- tioned otherwise, a... a voltage-independent K current that was blocked by clotrimazole (1 mM), an SK4 blocker. The expression of mRNAs for SK4 was confirmed in both Bal-17 and WEHI-231 cells. The density of clotrimazole-sensitive SK4 current was significantly larger in Bal-17 than WEHI-231 cells (11.4⁑3.1 Vs. 5.7⁑1.15 pA/pF). Also, the chronic stimulation of B cell receptors (BCR) by BCR-ligation (anti-IgM Ab, 3μg/ml, 8∼12 h) significantly upregulated the amplitude of clotrimazole-... -
Higher Expression of TRPM7 Channels in Murine Mature B Lymphocytes than Immature Cells
JinKyoungKim, JaeHongKo, JooHyunNam, Ji-EunWoo, KyeongMinMin, YungEEarm, SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.2 1 69-76 (8 pages)
of MIC was further increased, and the relation between current and voltage beca me linear under divalent cation-free conditions, demonstrating typical properties of the TRPM7. The stimulation of B cell receptors (BCR) by ligation with antibodies did not change the amplitude of MIC current. Also, increase of extracellular [Mg 2+]c to enhance the Mg 2+ influx did not affect the BCR ligation-in... of MIC was further increased, and the relation between current and voltage became linear under divalent cation-free conditions, demonstrating typical properties of the TRPM7. The stimulation of B cell receptors (BCR) by ligation with antibodies did not change the amplitude of MIC current. Also, increase of extracellular [Mg2]c to enhance the Mg2 influx did not affect the BCR ligation-induced death of WEHI-231 cells. Although the level of TRPM7 was not directly related with the cell... -
Nitric Oxide (NO) Inhibites the Neuronal Activities in the Rat Nucleus Tractus Solitarius
MiWonKim, MunSungPark, SunYoulRyu, JiYeonJung, YeonJinJeong, Sun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.1 2 7-15 (9 pages)
a giga-ohm seal was formed and the patch ruptured, current stimulation protocols were performed and voltage data were acquired using the pCLAMP program (Axon Instruments). Current was in- jected into neurons using the br idge circuit of an Axoclamp 2B amplifier in current clam p mode. The indifferent elec- trode was Ag-AgCl wire connected to the extracellular solution. Neurons were separate d... by a decrease in input resistance. The hyperpolarization caused by SNAP or SNP increased the time to initiate the first action potential, and decreased the number of action potentials elicited by current injection. SNP or SNAP also markedly decreased the number of firing neural discharges of the spontaneous NTS neural activity under zero current. Superfusion of L-NAME, a NOS inhibitor, slightly depolarized the membrane potential and increased the firing rate of NTS neurons induced by current... -
Congenital LQT Syndromes From Gene to Torsade de Pointes
EdwardCarmeliet 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.1 1 1-8 (8 pages)
by a decrease of function caused by a variety of underlying mechanisms. A summary of the biophysical changes and corresponding mutations is given in Table 1 (the list of the mutations is not exhaustive). As major changes distinction is made between absence of function or change in function. Absence of function can be due to the presence of non-conducting channels in the plasma membrane or... Congenital Long QT syndrome (LQTs) is a relatively rare pathologic disorder but results frequently in sudden cardiac death. Of the six LQTs that have been clinically described, five have been worked out for their genetic and biophysical profile. Most are generated by mutations which cause a loss of function in two delayed Kcurrents, iKs and iKr. One syndrome is generated by mutations in the Na channel which causes essentially a gain of function in the channel. Clinically the... -
Blockade of Intrinsic Oscillatory Activity of Cerebellar Purkinje Cells by Apamin and Nickel
WhasookSeo, JeanC.Strahlendorf, HowardK.Strahlendorf 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.5 2 477-484 (8 pages)
Sugimori， 1980b). PC were current or voltage c1amped with a bridge or switching clap circuit (3 -5 KHz， 50% duty cycle， Dagan 8100). During voltage claping procedures， the mem brane potential was swept slowly (approximately 1 mV/sec) to allo、 voltage-and time-dependent currents to approach presumed quasi-steady state conditions TO Study Ca 2 1 d Ca 2 1 껴dependent K ' currents active... inhibited post-burst hyperpolarizations (PBHs) progressively and finally terminated oscillatory firing activity of PCs. Apamin did not affect the amplitude or duration of the after-hyperpolarization (AHP) between spikes within the burst. In the voltage clamp mode, apamin shifted the whole-cell, quasi-steady state I/V relationship in an inward direction and abolished the zero slope resistance (ZSR) region by blocking outward current. Nickel (Ni2) terminated oscillatory activity and also...