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Genomic diversity of the Avian leukosis virus subgroup J gp85 gene in different organs of an infected chicken
Fanfeng Meng, Xue Li, Jian Fang, Yalong Gao, Lilong Zhu, Guiju Xing, Fu Tian, Yali Gao, Xuan Dong, Shuang Chang, Peng Zhao, Zhiz 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 8 497-503 (7 pages)
Sci China Life Sci 2013, 56, 414-420. 13. Nichol S. RNA viruses. Life on the edge of catastrophe. Nature 1996, 384, 218-219. 14. Payne LN, Brown SR, Bumstead N, Howes K, Frazier JA, Thouless ME. A novel subgroup of exogenous avian leukosis virus in chickens. J Gen Virol 1991, 72, 801-807. 15. Payne LN, Howes K, Gillespie AM, Smith LM. Host range of Rous sarcoma virus pseudotype RSV(HPRS-103)... of Avian leukosis virus subgroup J (ALV-J) was investigated in an experimentally infected chicken. ALV-J variants in tissues from four different organs of the same bird were re-isolated in DF-1 cells, and their gp85 gene was amplified and cloned. Ten clones from each organ were sequenced and compared with the original inoculum strain, NX0101. The minimum homology of each organ ranged from 96.7 to 97.6%, and the lowest homology between organs was only 94.9%, which was much lower than the 99.1%... -
Detection of Corynebacterium bovis infection in athymic nude mice from a research animal facility in Korea
Tae-Hyoun Kim, Dong-Su Kim, Ju-Hee Han, Seo-Na Chang, Kyung-Sul Kim, Seung-Hyeok Seok, Dong-Jae Kim, Jong-Hwan Park, Jae-Hak Par 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 13 583-586 (4 pages)
and nine Persian). Each subgroup (n = 9) was subdivided based on age: less than 1 year old (n = 2), 1∼3 years old (n = 4), and 3∼6 years old (n = 3). To reduce statistical error, breeds that were underrepresented in the study population (Turkish Angora and Siamese cats) were excluded from the analysis (Table 1). Anesthesia All cats were anesthetized with medetomidine hydrochloride (40... Corynebacterium (C.) bovis infection in nude mice causes hyperkeratosis and weight loss and has been reported worldwide but not in Korea. In 2011, nude mice from an animal facility in Korea were found to have white flakes on their dorsal skin. Histopathological testing revealed that the mice had hyperkeratosis and Gram-positive bacteria were found in the skin. We identified isolated bacteria from the skin lesions as C. bovis using PCR and 16S rRNA sequencing. To the best of our knowledge, this... -
The critical time of avian leukosis virus subgroup J-mediated immunosuppression during early stage infection in specific pathogen-free chickens
Feng Wang, Xiaowei Wang, Hongbo Chen, Jianzhu Liu, Ziqiang Cheng* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 6 235-241 (7 pages)
AATGGTGAGGTCGCTGA- CTGT; GAPDH forward (S1), GAACATCATCCCAG- CGTCCA; GAPDH reverse (S2), CGGCAGGTCAGG- TCAACAAC. The PCR was performed in a 50 μL reaction containing 25 μL 2× PCR master mix (Promega, USA), 2 ..PAGE:3 Immunosuppression by avian leukosis virus subgroup J 237 Fig. 1. Dynamic change of body weight and relative weight of immune organs. (A) 100% of the avian leukosis virus subgroup... The critical time of avian leukosis virus subgroup J (ALV-J)-mediated immunosuppression was determined by body weight, relative immune organ weight, histopathology, and presence of group specific antigen and antibodies in specific pathogen-free (SPF) chickens. CD4 and CD8 cell activity in the spleen, total and differential leukocyte counts in blood, and viral RNA levels in spleen were measured. Significant growth suppression was observed in the two ALV-J-infected groups. A strong immune response... -
Changes in orexin-A and neuropeptide Y expression in the hypothalamus of the fasted and high-fat diet fed rats
Eun Sung Park, Seong Joon Yi, Jin Sang Kim, Heungshik S. Lee, In Se Lee, Je Kyung Seong, Hee Kyung Jin, Yeo Sung Yoon 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 3 295-302 (8 pages)
in each fasting subgroup. Data were represented as means ± S.E.M. Five rats were used in each fasting subgroup. **; p<0.01. Fig. 2. Regression model of the mean loss body weights of each fasting subgroup. Fig. 3. Comparison of the mean body weight gain of the high-fat and normal-fat diet fed groups. Data were represented as means ± S.E.M. Five rats were used in each group. *; p<0.05. Fig. 4.... h of fasting. The number of OXA-IR neurons of the LHA (lateral hypothalamic area) in the high-fat (HF) diet fed group was more increased than that of the same area in the normal-fat (NF) diet fed group. The NPY immunoreactivities of the ARC and the SCN were higher in HF group than those observed in the same areas of NF group. Based on these results, it is noteworthy that the decrease of the body weight during the fast was not proportionate to the time-course, implicating a possible adaptation of... -
A study on the immunocytochemical localization of eurofascin in rat sciatic nerve
Byung-joon Chang, Ik-hyun Cho and Peter J. Brophy 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 1 67-71 (5 pages)
to a highly conserved stretch of amino acids. The intracellular segment of neurofascin as well as those of other members of the L1 subgroup interact with the cytoskeletal component ankyrin [4,6,7]. To elucidate the exact localization of neurofascin in the peripheral nerves is important to clarify the function of this molecule. In this report we have examined the ultrastructural localization of... the apposing membrane of paranodal loops. Axoplasm, compact myelin and cytoplasm of Schwann cell do not express NF at all. In the Schmidt-Lanterman incisures, NF is expressed weakly along the Schwann cell membrane. We propose that neurofascin may be a plasmalemmal integral protein of Schwann cell in the paranode and plays some important roles for the maintenance of axo-glial junctions at the paranode. It may also have some roles for maintaining the structure of Schmidt-Lanterman incisure and hav... -
Prospective validation of a novel dosing scheme for intravenous busulfan in adult patients undergoing hematopoietic stem cell transplantation
Sang-HeonCho, Jung-HeeLee, Hyeong-SeokLim, Kyoo-HyungLee, Dae-YoungKim, SangminChoe, Kyun-SeopBae, Je-HwanLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.3 3 245-251 (7 pages)
the subgroup that received the new dosing scheme, these values were -2.44% and 31.15%, respectively. A box plot of the AUC PRED in the validation group using the population model is shown in Fig. 3. The MPE and RMSE values of AUCPRED in all of the included patients were 2.0% and 17.7%, respectively. Within the conventional dosing scheme subgroup, the RMSE of AUC PRED was 20.6%, this value was... by a visual predictive check (VPC). Maximum a posteriori Bayesian estimators were estimated to calculate the predicted AUC (AUCPRED). The accuracy and precision of the AUCPRED values were assessed by calculating the mean prediction error (MPE) and root mean squared prediction error (RMSE), and compared with the target AUC of 5924 μM∙min. VPC showed that most data fell within the 95% prediction interval. MPE and RMSE of AUCPRED were -5.8% and 20.6%, respectively, in the conventional dosing... -
Cardiac Damage Biomarkers Following a Triathlon in Elite and Non-elite Triathletes
Chan-HoPark, Kwi-BaekKim, JinHan, Jin-GooJi, Yi-SubKwak 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.5 10 419-423 (5 pages)
known, however the effect of Olympic triathlon on cardiac damage in elite and non-elite athletes has not been evaluated. In the present study, we found that cardiac damage occurs during a tri- athlon race and elite triathletes had more improvement than the non-elite triathletes. However, all cardiac damage markers return to normal range within 1 week. Young athletes are widely regarded as a... (Hb) was significantly decreased in the NEG (p＜.05) only 2 hours after the race. Although cardiac troponin T (cTnT) was significantly increased in the EG but not in the NEG 2hours after the race (p＜.05), there was no group-by-time interaction. cTnT was completely recovered in both groups 7 days after the race. In conclusion, cardiac damage occurs during a triathlon race and, is greater in elite than in non-elite. However, all cardiac damage markers return to normal range within 1 week. -
Cyclophilin A as a New Therapeutic Target for Hepatitis C Virus- induced Hepatocellular Carcinoma
JinhwaLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.5 1 375-383 (9 pages)
C virus, Hepatocellular carcinoma, Peptidyl prolyl isomerase INTRODUCTION Cyclophilins (Cyps) constitute a subgroup of large family proteins called immunophilins. Cyps are well conserved and are found throughout evolution from E. coli to humans. Cyclosporine A (CsA) is a reversible inhibitor for all Cyps and works at nanomolar concentr ations [1,2]. Cyps possess a peptidyl prolyl is omerase... A, is overexpressed in many cancer types and often associated with malignant transformation. Therefore, CypA can be a good target for molecular cancer therapy. Because of antiviral activity, the CypA inhibitors have been tested for the treatment of chronic hepatitis C. Nonimmunosuppressive Cyp inhibitors such as NIM811, SCY-635, and Alisporivir have attracted more interests for appropriating CypA for antiviral chemotherapeutic target on HCV infection. This review describes CypA inhibitors as a... -
Construction of Infectious cDNA Clone of a Chrysanthemum stunt viroid Korean Isolate
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 1호 8 68-74 (7 pages)
in Etrog citron but not in experimental herbaceous hosts. Mol. Plant Pathol. 12:203- 208. Navarro, B. and Flores, R. 1997. Chrysanthemum chlorotic mottle viroid: unusual structural properties of a subgroup of self-cleaving viroids with hammerhead ribozymes. Proc. Natl. Acad. Sci. USA 94:1126211267. Owens, R. A., Erbe, R., Hadidi, A., Steere, R. I. and Diener, T. O. 1977. Infection of tomato... inoculation of plasmid cDNA clones containing full-length monomeric cDNA of CSVd-SK1 failed to infect tomato and chrysanthemum and linear negative-sense transcripts from the plasmid DNAs were not infectious in the two plant species. The cDNA sequences of progeny viroid in systemically infected tomato and chrysanthemum showed a few substitutions at a specific nucleotide position, but there were no deletions and insertions in the sequences of the CSVd progeny from tomato and chrysanthemum plants.