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The Current Status of Swine Respiratory Diseases in South Korea: Porcine Respiratory and Reproductive Viruses (PRRSVs) and Porcine Circovirus Type 2 (PCV)
Ga Eun Lee, Jung Ah Kim, Chan Hee Lee, Hee Chun Chung, Hye Jung Yang, Bong Kyun Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 53 111-112 (2 pages)
called thermogram. Skin temperature is affected by underlying blood circulation and tissue metabolism rate. Therefore, the changes in blood flow results in variation of surface temperature causing change in the amount of heat radiated. Heat change, which may relate to the inflammation of regional tissues or the alteration of metabolic activity, can be easily detected by IRT. The objective o... -
Isolation and characterization of a new porcine epidemic diarrhea virus variant that occurred in Korea in 2014
Dong-Kun Yang, Ha-Hyun Kim, Seung-Heon Lee, Soon-Seek Yoon, Jung-Won Park, In-Soo Cho 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 10 71-78 (8 pages)
the cytoplasm were considered positive. Reverse transcription-polymerase chain reaction Viral RNA extraction from 200 μL of the small intestinal homogenate samples was performed by using a viral RNA isolation kit (iNtRON Biotechnology, Korea). Viral RNA was eluted using 50 μL of elution buffer, and the eluted RNA was subjected to conventional reverse transcription-polymerase chain reaction... Outbreaks of porcine epidemic diarrhea (PED) have resulted in significant economic losses in the swine industry, and another PED outbreak occurred in 2014 in Korea. Isolating and culturing PED virus (PEDV) allow investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated two PEDV isolates (QIAP1401 and QIAP1402) from naturally infected piglets at Jeju-do, Korea. Viral propagation was confirmed in Vero cells based on... -
Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 9 59-70 (12 pages)
1 [7,31]. DNA extraction was performed according to standard protocols. Chromosomal DNA was extracted from B (Houston-1) by using DNeasy Blood & Tissue Kits (Qiagen, Germany) according to the manufacturer’s instructions. For construction of the omp43 plasmid (pomp43), the omp43 gene was amplified by using BamH-omp43 and omp43-Hind primers. The amplicon was cloned with pGEM-T Easy Vectors... Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among Bartonella henselae-derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than... -
Anti-melanogenic effects of black, green, and white tea extracts on immortalized melanocytes
Young Chul Kim, So Young Choi, Eun Ye Park 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 1 135-143 (9 pages)
and then treated with 5 mL of tea extracts (0∼ for 48 h. The cells were washed with phosphate buffered saline, detached with 200 L of 1% Triton X-100, transferred to Eppendorf tubes, subjected to extraction on ice with agitation six times every 10 min, and centrifuged at 18,620 × g for 20 min at 4 o C. Next, 100 L of L-DOPA were added and the mixture was incubated at 37 oC with 10% CO2 for ... Tea contains polyphenols and is one of the most popular beverages consumed worldwide. Because most tyrosinase inhibitors that regulate melanogenesis are phenol/catechol derivatives, this study investigated the inhibitory effects of Camellia sinensis water extracts (CSWEs), including black tea, green tea, and white tea extracts, on melanogenesis using immortalized melanocytes. CSWEs inhibited melanin accumulation and melanin synthesis along with tyrosinase activity in a concentration-dependent... -
Epidemiological characteristics of pulmonary pneumocystosis and concurrent infections in pigs in Jeju Island, Korea
Ki-Seung Kim, Ji-Youl Jung, Jae-Hoon Kim, Sang-Chul Kang, Eui-Kyung Hwang, Bong-Kyun Park, Dae-Yong Kim, Jae-Hoon Kim* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 3 15-19 (5 pages)
(Invitrogen, USA), and supernatants were stored at 70 o C until use. RNA and DNA were extracted from 200 μL of supernatants using an RNeasy Protect mini-kit (Qiagen, Germany) and a G-spin DNA extraction kit (iNtRON Biotechnology, Korea), respectively. All analyses were carried out using a Dice TP600 PCR thermal cycler (TaKaRa, Japan). For the RT-PCR, 2 μL of the RNA samples were added to... Epidemiological characteristics of swine pulmonary Pneumocystis (P.) carinii and concurrent infections were surveyed on Jeju Island, Korea, within a designated period in 172 pigs submitted from 54 farms to the Department of Veterinary Medicine, Jeju National University. The submitted cases were evaluated by histopathology, immunohistochemistry, PCR/RT-PCR, and bacteriology. P. carinii infection was confirmed in 39 (22.7%) of the 172 pigs. Histopathologically, the lungs had moderate to severe... -
Survey of Helicobacter infection in domestic and feral cats in Korea
Heh-Myung Ghil, Jong-Hyeon Yoo, Woo-Sung Jung, Tae-Ho Chung, Hwa-Young Youn, Cheol-Yong Hwang* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 10 67-72 (6 pages)
a MEGAquick-spin gel extraction kit (Intron, Korea) to confirm the identity of the target gene PCR product. Purified PCR products were analyzed using an ABI 3100 automatic sequence analyzer (Applied Biosystems, USA). Results Helicobacter genus-specific PCR On Helicobacter genus-specific PCR for 16s rRNA gene, 36 (56.3%) from 64 domestic cats were positive, and 92 (91.1%) from 101 feral cats... fundamental change in our understanding of gastric diseases in humans. Previous studies have found various Helicobacter spp. in dogs and cats, and pets have been questioned as a zoonotic carrier. The present study surveyed the Helicobacter infections and investigated the presence of H. felis and H. pylori infections in domestic and feral cats in Korea. Sixty-four domestic cats and 101 feral cats were selected from an animal shelter. Saliva and feces were evaluated by Helicobacter genus-specific... -
Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2
Sook-Yeon Lee, Jin-Young Chung, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Hwa-Young Youn, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 8 141-145 (5 pages)
the mRNA extraction was conducted at 70 oC freezer. Total RNA extraction and synthesis of cDNA The spinal tissue (30 mg) was disrupted in 1.5 ml tube with 350l of lysis buffer (Macherey-Nagel, Germany) and was ground with automatic homogenizer. Total RNA was isolated from spinal tissue with RNA extraction kit (Macherey-Nagel, Germany). Full-length first strand cDNA was prepared from total RNA with... The research of p53 is being conducted to find the mechanisms of tumorigenesis and to treat various cancers. Homeodomain-interacting protein kinase2 (HIPK2) is an important factor to regulate p53 and to increase the stability of p53. Activation of HIPK2 leads to the selective phosphorylation of p53, resulting in growth arrest and the enhancement of apoptosis. In this study, the canine HIPK2 cDNA fragments were obtained, and their overlapping regions were aligned to give a total sequence of 3489... -
Phenotype of peroxisome proliferator-activated receptor-α(PPARα)deficient mice on mixed background fed high fat diet
Bang Hyun Kim, Young Suk Won, Eun-Young Kim, Mijung Yoon, Ki-Taek Nam, Goo Taeg Oh, Dae-Yong Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 7 239-244 (6 pages)
proteinase K (100 g/ml) in digestion buffer (0.45% Tween 20, 0.45% NP- 40, 50 mM KCl, 10 mM TrisCl, 2.5 mM MgCl 2) overnight at 55 oC followed by extraction with phenol/chloroform and ethanol precipitation. The nucleotide sequences of PCR primers used for genotyping were the PPAR forward primer, 5'-GAGAAGTTGCAGGAGGGGATTGTG-3', the reverse primer, 5'-CCCATTTCGGTAGCAGGTAGTCTT-3', and the NEO primer... to establish the phenotypic characterization of PPARα-/- mice prior to the obesity-related study. In this study, we observed phenotype of PPARα-/- mice on mixed genetic background (C57BL/6N X 129/Sv) fed a high fat diet for 16 weeks. PPARα-/- mice, regardless of sex, raised body growth rate significantly comparing with wild type and showed male-specific fatty change in the liver. They were shown to lack hepatic induction of PPARα target genes encoding enzymes for fatty acid β-oxidation. -
Original Article : rpoB gene sequencing for phylogenetic analysis of avian pathogenic Escherichia coli
Hyuk Joon Kwon , Won Jin Seong , Tae Eun Kim , Yong Jin Won , Jae Hong Kim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 1호 5 31-39 (9 pages)
SD: Shigella dysenteriae, SF: Shigella flexneri. ..PAGE:3 Phylogenetic analysis of APEC 33 designed manually (Table 2). PCR and sequencing Total APEC DNA was extracted with a G-spin genomic DNA extraction kit (iNtRON Biotechnology, Korea) in accordance with the manufacturer’s instructions. The PCR solution was composed of 10 × buffer (5 L), dNTPs (2.5 mM, 1 L), forward and reverse primers (10... -
Efficacy of aromatherapy for the treatment of otitis externa in dogs
Sang Hun Kim , Suk Kim , Hyung Kyou Jun , Duck Hwan Kim , 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2009, 제 49권 제 1호 13 85-89 (5 pages)
systemic therapy and surgery [5, 10, 12, 18]. Essential oils are volatile and lipophilic substances that are obtained from plant materials through distillation, mechanical separation, or solvent extraction [1, 17]. The oils are mainly hydrocarbon metabolites of monoterpenes and sesquiterpenes, phenylpropanoids, amino acid and fatty acids [1, 17]. Aromatherapy is the therapeutic use of volatile...