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Design of Quorum Quenching Microbial Vessel to Enhance Cell Viability for Biofouling Control in Membrane Bioreactor
Cheong. Won-Suk, Kim. Sang-Ryoung, Oh. Hyun-Suk, Lee. Sang H., Yeon. Kyung-Min, Lee. Chung-Hak, Lee. Jung-Kee 한국미생물생명공학회 Journal of microbiology and biotechnology 9 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2014, Vol.24 No.1 97-105 (9 pages)
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Fuzzy Logic Control of Rotating Drum Bioreactor for Improved Production of Amylase and Protease Enzymes by Aspergillus oryzae in Solid-State Fermentation
Sukumprasertsri. Monton, Unrean. Pornkamol, Pimsamarn. Jindarat, Kitsubun. Panit, Tongta. Anan 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2013, Vol.23 No.3 335-342 (8 pages)
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Cultivation of Foot-and-Mouth Disease Virus vaccine candidates of serotype O in bioreactor
Ji Hyeon Hwang, Kwang Nyeong Lee, Min Yeong Kim, Jong Hyeon Park, Byoung han Kim 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 210 307-307 (1 pages)
cell was cultivated in ProVero media. Before seeding the cell in the bioreactor, pH and pO2 probes were calibrated and vessel pipelines were connected to the tubes and filters before vessel sterilization(121 oCin 15 min) and dehydrateion of the filter for 24h . Then vessel connected to the control tower and probes were re-calibrated. After feeding media, cells were seeded in the vessel with... -
Control Efficacy of Bacillus velezensis AFB2-2 against Potato Late Blight Caused by Phytophthora infestans in Organic Potato Cultivation
Min Jeong Kim, Chang Ki Shim, Jong-Ho Park 한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 8 580-595 (16 pages)
NaCl. After inoculation, the bacterial growth was evaluated using the RTS-8 Multi-channel Bioreactor (Bio- san Co. Ltd.) at 37°C for 25 h. The absorbance of the cul- ture was evaluated automatically at the 30-min interval at 600 nm in the RTS-8 Multi-channel Bioreactor. The high- est OD value obtained at different NaCl concentrations was considered as the maximum salinity tolerance capacity.... of biological control technology is low. It is a necessary to develop an effective biocontrol agent to inactivate late blight in the field. AFB2-2 strain is a gram-positive with peritrichous flagella. It can uti- lize 20 types of carbon sources, like L-arabinose, and D- trehalose at 35°C. The optimal growth temperature of the strain is 37°C. It can survive at 20-50°C in tryptic soy broth. The maximum salt concentration tolerated by AFB2-2 strain is 7.5% NaCl. AFB2-2 strain inhib- ited the... -
Biological Control of Root-Knot Nematodes by Organic Acid-Producing Lactobacillus brevis WiKim0069 Isolated from Kimchi
Hye Jeong Seo, Ae Ran Park, Seulbi Kim, Jehyeong Yeon, Nan Hee Yu, Sanghyun Ha, Ji Yoon Chang, Hae Woong Park, Jin-Cheol Kim 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 12 662-673 (12 pages)
jar bioreactor (5 l, MARADO-05D-XS, BioCnS, Daejeon, Korea). The cul- tivation was conducted at 30°C for 24 h with an agitation speed of 300 rpm and an aeration rate of 1.0 vvm (volume of air added to the liquid volume per minute). The culture broth was centrifuged at 5,000 rpm for 10 min at 4°C, and the supernatant was filtered (0.45 m) and injected into a chromatograph with a loop of 50... Root-knot nematodes (RKNs) are among the most destructive plant-parasites worldwide, and RKN control has been attempted mainly using chemical nematicides. However, these chemical nematicides have negative effects on humans and the environment, thus necessitating the search for eco-friendly alternative RKN control methods. Here, we screened nematicidal lactic acid bacteria (LAB) isolated from kimchi and evaluated their efficacy as biocontrol agents against RKNs. Of 237 bacterial strains,... -
Development of a Biofungicide Using a Mycoparasitic Fungus Simplicillium lamellicola BCP and Its Control Efficacy against Gray Mold Diseases of Tomato and Ginseng
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 12 337-344 (8 pages)
to a 5,000-l pilot bioreactor, optimization of the freeze-drying of the fermentation broth, and preparation of a wettable powder-type formulation were performed. Then, its disease control efficacy was evaluated against gray mold diseases of tomato and ginseng plants in field conditions. The final conidial yields of S. lamellicola BCP were 3.3 × 10 9 conidia/ml for a 5-l jar, 3.5 × 10 9... jar to a 5,000-l pilot bioreactor, optimization of the freeze-drying of the fermentation broth, and preparation of a wettable powder-type formulation were performed. Then, its disease control efficacy was evaluated against gray mold diseases of tomato and ginseng plants in field conditions. The final conidial yields of S. lamellicola BCP were 3.3 × 10 conidia/ml for a 5-l jar, 3.5 × 10 conidia/ml for a 500-l pilot vessel, and 3.1 × 10 conidia/ml for a 5,000-l pilot... -
Sensitive detection method of Foot-and-mouth disease virus in pigs by oral-fluid using cotton rope
Tae seong Kim, So yoon Ryoo, Jin ju Nah, Min geun Sagong, Su mee Lee, Ki Sik Choung, Sung Hwan Wee, Bok Kyung Ku 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 211 307-308 (2 pages)
cell was cultivated in ProVero media. Before seeding the cell in the bioreactor, pH and pO2 probes were calibrated and vessel pipelines were connected to the tubes and filters before vessel sterilization(121 oCin 15 min) and dehydrateion of the filter for 24h . Then vessel connected to the control tower and probes were re-calibrated. After feeding media, cells were seeded in the vessel with... -
Heterosubtypic protection of a live H1N1 influenza vaccine against H5N1 virus infection
Chung Young Lee, Se Hee Ahn, Jae Hong Kim, Hyuk Joon Kwon 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 209 306-307 (2 pages)
cell was cultivated in ProVero media. Before seeding the cell in the bioreactor, pH and pO2 probes were calibrated and vessel pipelines were connected to the tubes and filters before vessel sterilization(121 oCin 15 min) and dehydrateion of the filter for 24h . Then vessel connected to the control tower and probes were re-calibrated. After feeding media, cells were seeded in the vessel with... -
Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)
a mammary gland bioreactor, hLYZ-positive hTERT-bMGEs were used as donor cells for SCNT as described above. The rate of cleavage, blastocysts and hatched blastocysts were used for statistical analysis. The non-transfected hTERT-bMGEs were used as a control. Statistical analysis In this study, the ratio of egg cleavage, blastocyst formation and cell apoptosis was evaluated using the SPSS... used as a control group. The results showed that the hTERT-bMGEs group had much higher transgenic efficiency and protein expression than the bMGEs group. Furthermore, the nontransgenic and transgenic hTERT-bMGEs were used as donor cells to evaluate the efficiency of SCNT. There were no significant differences in rates of cleavage or blastocysts or hatched blastocysts of cloned embryos from nontransgenic hTERT-bMGEs at passage 18 and 28 groups (82.8% vs. 81.9%, 28.6% vs. 24.8%, 58.6% vs. 55.3%,... -
Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus)
Sang Tae Shin, *, Sung Keun Jang, Hong Suk Yang, Ok Keun Lee, Yhong Hee Shim, Won Il Choi, Doo Soo Lee, Gwan Sun Lee, Jong Ki Cho 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 14 103-107 (5 pages)
the best available bioreactor for the production of human pharmaceutical proteins [4,5,13,21]. Dairy goats have been used as a bioreactor because of their relatively short gestation period and low maintenance costs compared to cattle. Many studies have reported on the production of transgenic goats [6-8,11]. The possibility of large-scale production for industrial application has been demon-... This study was performed to produce transgenic Korean native goat (Capra hircus) by laparoscopic embryo transfer (ET) to overcome the limitations of ET performed by laparotomy. Transgenic embryos were produced by DNA pronuclear microinjection of in vivo zygotes. The recipient goats were synchronized for estrus by using an introvaginal progesterone devices as a controlled internal drugreleasing insert (CIDR) for 13 days and injection of 400 IU PMSG 48 h before removal of the insert. Embryos were...