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Inhibition of DNA-dependent Protein Kinase by Blocking Interaction between Ku Complex and Catalytic Subunit of DNA-dependent Protein Kinase
Chung-HuiKim, DangVanCuong, Jong-SuKim, NariKim, EuiyongKim, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.1 3 9-14 (6 pages)
protein kinase (DNA- PK), which has homologous ki nase domain. DNA-PK is a nuclear serine/threonine protein kinase composed of DNA binding and catalytic subunits. The DNA binding subunit is heterodimer of Ku70 and Ku80, that binds DNA ends and nicks, and has structures co ntaining a transition fork between dsDNA and two single strand. The catalytic subunit of DNA-PK (DNA-PKcs) is a 470-kDa... activities. For example, Ku heterodimer regulatory DNA binding subunits (Ku70/Ku80) on binding to double strand DNA breaks (DSBs) are able to interact with 470-kDa DNA-dependent protein kinase catalytic subunit (DNA-PKcs), and are essential for DNA-dependent protein kinase (DNA-PK) activity. It has been known that DNA-PK is an important factor for DNA repair and also is a sensor-transmitting damage signal to downstream targets, leading to cell cycles arrest. Our ultimate goal is to develop a... -
A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer
Qing Wang, Mianmian Chen, Wei Zhang 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 12 260-270 (11 pages)
Table 3, cellular and metabolic processes were the dominant subcategories in the biological process category, cell and cell part were the most abundant in the cellular component category, and catalytic activity and binding were highly represented in the molecular function category. In KEGG pathway annotation, 313 DEGs were represented among the pathway annotations, with metabolic pathways,... Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction... -
Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue
Masahiro Yasuda, Jyunya Kawabata, Sayaka Akieda-Asai, Tetsuo Nasu, Yukari Date 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 11 341-348 (8 pages)
of GC-C [5]. GNY binds to the extracellular binding domain of GC-C and activates the intracellular catalytic domain, regulating ion transportation and pH in the mucosa of several mammalian species. In rodents and humans, GC-C and GNY are reportedly expressed in various organs in addition to the intestine, such as adrenal gland, kidney, and ovary [25], suggesting that the GNY/GC-C system has... Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several mammalian species. The GNY/GC-C system regulates ion transportation and pH in the mucosa. Recently, it was reported that GC-C and GNY are involved in lipid metabolism in rat mesenteric adipose tissue macrophages. To examine the role of GC-C... -
Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Alisha Wehdnesday Bernardo Reyes, Hannah Leah Tadeja Simborio, Huynh Tan Hop, Lauren Togonon Arayan, Suk Kim 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 16 119-122 (4 pages)
Enzymatic activity analysis and catalytic essential residues identification of Brucella abortus malate dehydrogenase. ScientificWorldJournal 2014, 2014, 973751. 6. Ko J, Splitter GA. Molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans. Clin Microbiol Rev 2003, 16, 65-78. 7. Lee JJ, Simborio HL, Reyes AWB,... The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use... -
Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus
Wei Sun, Xiaokai Song, Ruofeng Yan, Lixin Xu, Xiangrui Li* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 8 49-58 (10 pages)
are in uppercase letters. The initiation ATG and stop TAG codons are shaded. Distal polyadenylation signal sequence AATGAA is shaded. The nucleotides and amino acids are numbered along the left margins. Conserved residues of the sequence are shown in boxes. The catalytic triad active sites are shaded. The spliced leader sequence is indicated in shadow. polyadenylation site was identified as a... targeting the 5´- and 3´-ends of the partial mRNA sequence. The cloned HC29 cDNA was shown to be 1,113 bp in size with an open reading frame of 507 bp, encoding a protein of 168 amino acid with a calculated molecular mass of 18.9 kDa. Amino acid sequence analysis revealed that the cloned HC29 cDNA contained the conserved catalytic triad and dimer interface of selenium-independent glutathione peroxidase (GPX). Alignment of the predicted amino acid sequences demonstrated that the protein shared... -
Expression and regulation of Enpp2 in rat uterus during the estrous cycle
Hyo-Jin Ahn, Hyun Yang, Beum-Soo An, Kyung-Chul Choi, Eui-Bae Jeung* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 11 379-385 (7 pages)
22. Koike S, Keino-Masu K, Ohto T, Masu M. The N-terminal hydrophobic sequence of autotaxin (ENPP2) functions as a signal peptide. Genes Cells 2006, 11, 133-142. 23. Lee HY, Clair T, Mulvaney PT, Woodhouse EC, Aznavoorian S, Liotta LA, Stracke ML. Stimulation of ..PAGE:7 Enpp2 is regulated by steroid hormones 385 tumor cell motility linked to phosphodiesterase catalytic site of autotaxin. J... Ectonucleotide pyrophosphatase/phosphodiestrase 2 (Enpp2) isolated from the supernatant of human melanoma cells is a lysophospholipase D that transforms lysophosphatidylcholine into lysophospatidic acid. Although multiple analyses have investigated the function of Enpp2 in the hypothalamus, its role in the uterus during the estrous cycle is not well understood. In the present study, rat uterine Enpp2 was analyzed by RT-PCR, Western blotting, and immunohistochemistry. Quantitative PCR analysis... -
Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2
Sook-Yeon Lee, Jin-Young Chung, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Hwa-Young Youn, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 8 141-145 (5 pages)
domain, a PEST sequence, a YH domain in the COOH-terminal and a protein kinase catalytic domain in the N-terminal side [10]. The enhancement of repressor activity of homeoproteins by HIPK2 is conferred by domains within the N-terminal half of the HIPK2. The SRS (nuclear speckle retention signal) that contains PEST sequence and YH domain has a positive and a negative effect on co-repressor activity... cDNA (GenBank accession number; AY800385) shares 93% and 90% sequence identity with those of human and mouse HIPK2, respectively. The canine HIPK2 cDNA contains an open reading frame encoding 1163 amino acid residues and the predicted amino acid sequence has 98% and 96% identity with those of human and mouse, respectively. The deduced amino acid sequence of canine HIPK2 has also all domains’ sites compared with human and mouse HIPK2. Therefore, these structural similarities suggested that the... -
Activation domain in P67phox regulates the steady state reduction of FAD in gp91phox
Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 4 27-31 (5 pages)
interacting directly with this catalytic component. Role of the activation domain in p67 phox in FAD reduction: As shown in Fig. 2 and 3, p67 phox derivatives which have an activation domain showed both higher rate of superoxide generation and higher level of steady state reduction of 8- thioacetamido-FAD. However, the truncation of the activation domain resulted in much lower rate of superoxide... An activation domain in p67(phox) (residues 199-210) is critical for regulating NADPH oxidase activity in cell-free system [10] To determine the steady state reduction of FAD, thioacetamide-FAD was reconstituted in gp91(phox), and the fluorescence of its oxidised form was monitored. Omission of p67(phox) decreased the steady state reduction of the FAD from 28% to 4%, but omission of p47(phox) had little effect. A series of the truncated forms of p67(phox) were expressed in E.coli to determine... -
Expression and characterization of the flavoprotein domain of gp91phox
Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 3 19-26 (8 pages)
b 558 which lacks the transmembrane heme-binding regions, and to investigate its catalytic properties. gp91 phox (306~569) includes most of the predicted b-stranded barrel structure including the HPFT motif and also contains regions which are predicted to form the NADPH binding site (Fig.1). This structure was successfully expressed as a TRX fusion protein and showed low catalytic activity (NBT... the NBT reductase activity, but p47(phox) had no effect. Truncated p67(phox) containing the activation domain (residues 199- 210) stimulated activity approximately 2-fold, whereas forms mutated or lacking this region failed to stimulate the activity. Our data indicate that: 1) TRX-gp91(phox) (306-569) contains the binding sites for both pyridine and flavin nucleotides; 2) this flavoprotein domain shows NBT reductase activity; and 3) the flavin-binding domain of gp91(phox) is the target of... -
Original Article : Virulence factors, antimicrobial resistance patterns, and genetic characteristics of hydrogen sulfide-producing Escherichia coli isolated from swine
Hyun Eui Park , Min Kyoung Shin , Hong Tae Park , Seung Won Shin , Myunghwan Jung , Young Bin Im , Han Sang Yoo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 3호 7 191-197 (7 pages)
coli and wild type E. coli should be needed to reveal the correlation between antibiotic resis- tance and H 2S production. sseA gene was detected in all isolates regardless of H2S production. Also, sequence of the sseA gene at motif CGS- GVTA around the catalytic cysteine (Cys238) was identical. These findings are contrasted with a previous research show- Fig. 1. Dendrogram of PFGE patterns of E....