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Optical sensitivity of DNA-dispersed single-walled carbon nanotubes within cement composites under mechanical load
Jin Hee Kim, Inkyu Rhee, Yong Chae Jung, Sumin Ha, Yoong Ahm Kim 한국탄소학회 Carbon Letters 7 Pages
한국탄소학회 Carbon Letters 2017, Vol.24 12 90-96 (7 pages)
was evaluated using com- bined in situ Raman and X-ray diffraction (XRD) studies. Fi- nally, we demonstrated the sensing ability of the optically active SWCNTs that were homogeneously dispersed in cement com- posites under mechanical compression. 2. Experimental Highly purifed HiPco-based SWCNTs with a diameter below 1 nm were used as a functional fller in cement composites [28]. To prepare a... 1 nm that were homogeneously dispersed in cement composites under a mechanical load. Deoxyribonucleic acid (DNA) was selected as the dispersing agent to achieve a homogeneous dispersion of SWCNTs in an aqueous solution, and the dispersion state of the SWCNTs were characterized using various optical tools. It was found that the addition of a large amount of DNA prohibited the structural evolution of calcium hydroxide and calcium silicate hydrate. Based on the in-situ Raman and X-ray diffraction... -
Carbon nanospheres synthesized via solution combustion method: their application as an anode material and catalyst for hydrogen production
Vivek Dhand, M Venkateswer Rao, J. S. Prasad, Garima Mittal, Kyong Yop Rhee, Hyeon Ju Kim, Dong Ho Jung 한국탄소학회 Carbon Letters 5 Pages
한국탄소학회 Carbon Letters 2014, Vol.15 No.3 7 198-202 (5 pages)
of dispersed carbon particles during pyrolysis of ethylene, benzene, and naph- thalene in a refected shock wave. Combust Explos Shock Waves, 43, 442 (2007). http://dx.doi.org/10.1007/s10573-007-0060-z. [7] D’Anna A, Commodo M, Violi S, Allouis C, Kent J. Nano or- ganic carbon and soot in turbulent non-premixed ethylene fames. Proc Combust Inst, 31, 621 (2007). http://dx.doi.org/10.1016/j.... range of 10-50 nm were synthesized using the solution combustion method. High-resolution transmission electron microscopy (HRTEM) revealed a densely packed high surface area of SP2-hybridized carbon; however, there were no crystalline structural components, as can be seen from the scanning electron microscopy, HRTEM, X-ray diffraction, Raman spectroscopy, and thermal gravimetric analyses. Electrochemical and thermo catalytic decomposition study results show that the material can be used as a... -
Dielectrophoretic Alignment and Pearl Chain Formation of Single-Walled Carbon Nanotubes in Deuterium Oxide Solution
Dong Su Lee, Yung Woo Park 한국탄소학회 Carbon Letters 6 Pages
한국탄소학회 Carbon Letters 2012, Vol.13 No.4 9 248-253 (6 pages)
Current leakage was observed and the leakage level was similar for both the the deuterium water so- lution containing only SDS and the SWCNTs-deuterium oxide solution. This implies that the leakage is due to ionic conduction in the deuterium oxide solution without any contribution of SW- CNTs. Thus, we can conclude that the SWCNTs are randomly dispersed and do not make a conduction path in the... -
Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Alisha Wehdnesday Bernardo Reyes, Hannah Leah Tadeja Simborio, Huynh Tan Hop, Lauren Togonon Arayan, Suk Kim 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 16 119-122 (4 pages)
DH5 competent cells, dispersed onto LB agar plates, and incubated at 37 o C for 16 to 18 h. The bacteria expressing the fusion plasmid were cultured overnight and transferred into ampicillin-containing LB broth. PCR amplifications were performed using primers specific for the mdh gene and colonies containing the recombinant plasmid were selected. For confirmation, the recombinant plasmid was... The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use... -
Protective effect of butylated hydroxylanisole against hydrogen peroxide-induced apoptosis in primary cultured mouse hepatocytes
Geun Hye Hwang, Yu Jin Jeon, Ho Jae Han, Soo Hyun Park, Kyoung Min Baek, Woochul Chang, Joong Sun Kim, Lark Kyun Kim, You-Mie Le 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 3 17-23 (7 pages)
Ca 2 + or SO4 containing 0.02% collagenase and 0.1 mM CaCl2 until the liver appeared soft. Next, the liver was removed and gently minced, and the released cells were dispersed in Dulbecco’s modified Eagle medium (DMEM; Life Technologies) containing 10% FBS and 1% penicillin/streptomycin (Life Technologies). The solution containing the mixed cells and debris was passed through a 100-m cell... Butylated hydroxyanisole (BHA) is a synthetic phenolic compound consisting of a mixture of two isomeric organic compounds: 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole. We examined the effect of BHA against hydrogen peroxide (H2O2)-induced apoptosis in primary cultured mouse hepatocytes. Cell viability was significantly decreased by H2O2 in a dose-dependent manner. Additionally, H2O2 treatment increased Bax, decreased... -
A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis
Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)
to remove the remaining liquid. The pellet was dispersed by pipetting with a mixture of 500 μL lysis buffer (2 mM EDTA, 400 mM NaCl, 10 mM Tris-HCl [pH 8.0], and 0.6% SDS) and 2 μL proteinase K (10 μg/μL; Sigma- Aldrich). The solution was then was transferred to a bead-beating tube (Biospec Products, USA) containing 200 μL of beads (0.1 mm zirconia/silica beads; Biospec Products) and... PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting DNA from Map cultured in liquid medium was developed. The proposed protocol (Universidad Austral de Chile [UACH]) was evaluated by comparing its performance to that of two traditional techniques (a QIAamp DNA Stool Mini Kit and cethyltrimethylammonium bromide [CTAB] method). The results were... -
A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
Sang Kyu Park, Sangho Roh*, Jong-Im Park* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 8 73-80 (8 pages)
a local slaughterhouse. The head, extremities, and internal organs were removed, and the remaining tissues were cut into small pieces (less than 1mm cube). Cells were dispersed by incubation for 20 min at 38.5 o C with 0.25% (v/v) trypsin solution and cultured in Dulbecco’s minimum essential medium supplemented with 10% (v/v) fetal bovine serum for 4 day at 38.5 o C in incubator. For each... Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated with demecolcine that facilitated enucleation with protruding genetic material. Enucleation and donor... -
Development of in vitro produced porcine embryos according to serum types as macromolecule
Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 11 315-321 (7 pages)
nuclear maturation than these of floating [31,32]. Similarly, the COCs of the ESS () and PSS () group were attached to the bottom of the culture dish and the oocytes were matured more early with dispersed cumulus cells in this study. Conversely, the COCs of NPS () and PGS () group were attached to the bottom of dish and the oocytes were surrounded by a compact somatic compartment. However,... This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient porcine serum during porcine in vitro production. To investigate the efficient porcine serum (PS), different types of PS [newborn pig serum, prepubertal gilt serum (PGS), estrus sow serum, and pregnancy sow serum] were used to supplement IVM media with or without gonadotrophin (GTH) and development rates of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were then... -
Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells
Min-Soo Seo, Sang-Bum Park, Hyung-Sik Kim, Jun-gu Kang, Joon-Seok Chae*, Kyung-Sun Kang* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 6 151-159 (9 pages)
arrow indicates the pellet formation (Fig. 5A). Pellet formation was not observed for cells grown under control conditions. The control cells dispersed when the medium was changed, indicating that chondrogenesis had not occurred with the basal culture medium. The pellet was positive for staining with toluidine blue (Fig. 5C) and Alcian blue-PAS (Fig. 5D). Discussion The placenta, which is... Recent studies have shown that mesenchymal stem cells (MSCs) are able to differentiate into multi-lineage cells such as adipocytes, chondroblasts, and osteoblasts. Amniotic membrane from whole placenta is a good source of stem cells in humans. This membrane can potentially be used for wound healing and corneal surface reconstruction. Moreover, it can be easily obtained after delivery and is usually discarded as classified waste. In the present study, we successfully isolated and characterized...


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