자료유형
간행물
- THE KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY(36)
- THE PLANT PATHOLOGY JOURNAL (14)
- JOURNAL OF VETERINARY SCIENCE(13)
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY(3)
- JOURNAL OF BACTERIOLOGY AND VIROLOGY : JBV(2)
- KOREAN JOURNAL OF VETERINARY RESEARCH(구 대한수의학회지)(2)
- 식물병연구(2)
- JOURNAL OF KOREAN BIOLOGICAL NURSING SCIENCE(1)
전체 선택해제
총 73건 검색
본문검색 결과만 보기
-
Expression and Purification of Human Farnesoid X Receptor-Ligand Binding Domain as Soluble Form Using a Dual Cistronic Expression Vector
Kang. Hyun, Ye. Micheal B., Bahk. Young Yil 한국미생물생명공학회 Journal of microbiology and biotechnology 7 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2013, Vol.23 No.3 322-328 (7 pages)
-
Expression of Human Thrombopoietin in Insect Cells by Polyhedrin-gp64 Dual Promoter-Based Baculovirus Vector System
Koh. Yeo-Wook, Park. Sang-Kyu, Kim. Kun-Soo, Kim. Seong-Ryong, Lee. Jeong-Kug, Yang. Jai-Myung 한국미생물생명공학회 Journal of microbiology and biotechnology 5 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 1999, Vol.9 No.3 318-322 (5 pages)
-
Mouse Dual Ig Domain Containing Cell Adhesion Molecule Protein Expression and Purification Using the Baculovirus Expression Vector System
Park. Seung-Won, Choi. Ji-Hyun, Goo. Tae-Won, Kim. Seong-Ryul, Lee. Gwang-Gill, Kang. Seok-Woo 대한미생물학회 Journal of bacteriology and virology : JBV 8 Pages
대한미생물학회 Journal of bacteriology and virology : JBV 2010, Vol.40 No.3 123-130 (8 pages)
-
Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene
Yuqiang Cheng, Yingjie Sun, Hengan Wang, Shuduan Shi, Yaxian Yan, Jing Li, Chan Ding, Jianhe Sun 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 6 37-46 (10 pages)
(pCAGGS-dTLR5 or empty vector) using Fugen HD. (C and D) HeLa (C) and DF-1 (D) cells were transfected with 0.1 g/well of pGL-IL-6-Luc along with 0.025 g/well of pRL-TK plasmid and th e expression constructs (pCAGGS-dTLR5 or empty vector). Twenty-four hour post-transfection, 50 ng/mL of flagellin was added to the transfected cells and luciferase assays were performed after stimulation for 6 h... Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2’-5’-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the... -
Aerosol delivery of kinase-deficient Akt1 attenuates Clara cell injury induced by naphthalene in the lungs of dual luciferase mice
Arash Minai-Tehrani, Young-Chan Park, Soon-Kyung Hwang, Jung-Taek Kwon, Seung-Hee Chang, Sung-Jin Park, Kyeong-Nam Yu, Ji-Eun Ki 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 2 309-317 (9 pages)
also has demonstrated the efficacy of aerosol gene delivery using GPEI by showing that aerosol delivery of Akt using GPEI could control Akt pathway and protein translation in the lungs of dual luciferase mice [41]. Based on those promising results, we chose to use GPEI as a ..PAGE:6 314 Arash Minai-Tehrani et al. Fig. 6. Effects of the Akt1 KD mutant and naphthalene on p70 S6K, p-p70 S6K ,... cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using glucosylated polyethylenimine and naphthalene was administrated to the mice via intraperitoneal injection. Aerosol delivery of Akt1 WT and naphthalene treatment increased protein levels of... -
Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis
Seungjoon Kim, Changjong Moon, Myung Bok Wie, Hyungmin Kim, Naoyuki Tanuma, Yoh Matsumoto, Taekyun Shin 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 2 11-17 (7 pages)
the ..PAGE:3 Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomelitis 13 appropriate biotinylated second antibody and the avidin- biotin peroxidase complex Elite kit (Vector, Burlingame, CA) were added sequentially. Peroxidase was developed with diaminobenzidine-hydrogen peroxidase solution (0.001% 3,3'-diaminbenzidine and 0.01% hydrogen... rats without EAE. Immunohistochemical study showed that the expression of either nNOS or eNOS increased in the brain cells including neurons and astrocytes during the peak and recovery stages of EAE, while the expression of iNOS was found mainly in the inflammatory macrophages in the perivascular EAE lesions. Double labeling showed that apoptotic cells had intimate contacts with either neurons or astrocytes, which are major cell types to express nNOS and eNOS constitutively. Our results suggest... -
Dual control of the vestibulosympathetic reflex following hypotension in rats
SangEonPark, Yuan-ZheJin, ByungRimPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.6 13 675-686 (12 pages)
( ‡p<0.01). ..PAGE:7 Dual control of the vestibulosympathetic reflex Korean J Physiol Pharmacol 2017;21(6):675-686 www.kjpp.net 681 group (p<0.01) (Fig. 4). Measurement of pERK using a Western blot To confirm the functional connectivity between the MVN and the IMC, following the administration of glutamate to the MVN, the level of pERK expression in the IMC of the T4~7 spinal regions was... sinoaortic denervation in order to induce hypotension. Expression of pERK in the intermediolateral cell column (IMC) of the T4~7 thoracic spinal regions, blood epinephrine levels, and blood pressure were evaluated following the administration of glutamate and/or SNP. SNP-induced hypotension led to increased pERK expression in the medial vestibular nucleus (MVN), rostral ventrolateral medullary nucleus (RVLM) and the IMC, as well as increased blood epinephrine levels. We co-administered either a... -
LPS Increases 5-LO Expression on Monocytes via an Activation of Akt-Sp1/NF-κB Pathways
SeungJinLee, KyoWonSeo, ChiDaeKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.3 11 263-268 (6 pages)
ese cells and found that the ..PAGE:2 264 SJ Lee, et al Akt is the major signaling pathway that contributes to TLR4- dependent 5-LO induction. Mo reover, an Akt pathway ap- pears to increase 5-LO expression through activation of the Sp1 and NF- κB transcription factors in monocytes. METHODS Chemicals and antibodies LPS from Escherichia coli was purchased from Sigma- Aldrich (Saint Louis, MO... LPS increased the phosphorylation of Akt, suggesting a role for the Akt pathway in LPS-induced 5-LO expression. In a promoter activity assay conducted to identify transcription factors, both Sp1 and NF-κB were found to play central roles in 5-LO expression in LPS-treated monocytes. The LPS-enhanced activities of Sp1 and NF-κB were attenuated by an Akt inhibitor. Moreover, the LPS-enhanced phosphorylation of Akt was significantly attenuated in cells pretreated with an anti-TLR4 antibody. Taken... -
Differential Gene Expression in GPR40-Overexpressing Pancreatic β-cells Treated with Linoleic Acid
In-SuKim, So-YoungYang, Joo-HuiHan, Sang-HyukJung, Hyun-SooPark, Chang-SeonMyung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.2 9 141-149 (9 pages)
o C with 5% CO 2. Vector constructs For construction of the pCMV6-Neo-GPR40 expression plasmid, the vector pEZ-M02-GPR40 was purchased from GeneCopoeia (MD, USA) and in serted into the pCMV6-Neo vector (ORIGENE, MD, USA). E. coli DH-5α cells harbor- ing the selected plasmid DNA were cultured in LB broth media. Plasmid DNA was extracted using a plasmid mini kit (Qiagen, Hilden, Germany). The... determined the gene expression profiles of RIN-5f and RIN-40. In total, 1004 genes, the expression of which was altered at least twofold, were selected in RIN-5f versus RIN-40. Moreover, the differential genetic profiles were investigated in RIN-40 cells treated with 30 μM linoleic acid, which resulted in selection of 93 genes in RIN-40 versus RIN-40 treated with linoleic acid. Based on the Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG, http://www.genome.jp/kegg/), sets of genes induced... -
cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus
SangBaeYoo, SeoulLee, JooYoungLee, Bom-TaeckKim, Jong-HoLee, JeongWonJahng 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.5 7 333-337 (5 pages)
after the icv Sp-cAMP. Results suggest that cAMP/PKA pathway may mediate the regulation of the PVN-nNOS expression depending on different feeding conditions. Key Words: Food deprivation, Gene expression, Neuronal nitric oxide synthase, Paraventricular nucleus, Rat INTRODUCTION Food deprivation decreases mRNA level of neuronal nitric oxide synthase (nNOS), the intracellular intensity of NADPH-... Gene expression of neuronal nitric oxide synthase (nNOS) changes in the hypothalamic paraventricular nucleus (PVN) depending on feeding conditions, which is decreased during food deprivation and restored by refeeding, and phosphorylated cAMP response element binding protein (pCREB) was suggested to play a role in its regulation. This study was conducted to examine if the fasting-induced down-regulation of the PVN-nNOS expression is restored by activation of cAMP-dependent protein kinase A...