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Comparisonbetween Dot-immunoblotting Assay and Clinical Sign Determination Method forQuantifying Avian Infectious Bronchitis Virus Vaccine
Seong su Yuk, Jung Hoon Kwon, Jin Yong Noh, Woo tack Hong, Gyeong Bin Gwon, Jei Hyun Jeong, Ha Na Youn, Chang Seon Song 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 188 434-435 (2 pages)
of the diluted virus in embryonated chicken eggs, and clinical signsin the embryos are considered to be evidence of viral propagation. In thisstudy, we conducted a do t-immunoblotting assay (DIA) to assess the titers of IBVvaccines, which originated from different pathogenic strains or attenuationmethods, and compared this method to the currently used method of ..PAGE:2 Vol. 55, No. 2... -
Genetic Characterization of Salmonella Enteritidis and Salmonella Typhimurium Isolated from Samples of Slaughtered Animals during 2003-2008 in Korea
Sia Kang, Heui Jin Kim, Suk Kyung Lim, Kichan Lee, Dong Chan Moon, Hee Soo Lee, Hyang Mi Nam 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 96 188-189 (2 pages)
or fecal samples from poultry farms and wild bird habitats. AIVs were isolated by embryonated SPF egg inoculation with samples and the presence of AIV was determined using a hemagglutination assay after incubating the eggs at 37 o C for 45 days. AIVs were subtypedusing reverse transc ription-polymerase chain reaction and DNA sequencing. Results: Of the137,628 samples, we isolated total 650... -
Phylogenetic Analysis of H9N2 Avian Influenza Viruses in Live Bird Market of Korea, 2012-2014
Joo jin Jeong, Hyun Mi Kang, Eun Kyoung Lee, Byung Min Song, Gyoung Beom Heo, Suk Chan Jung, Youn Jeong Lee 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 95 188-188 (1 pages)
or fecal samples from poultry farms and wild bird habitats. AIVs were isolated by embryonated SPF egg inoculation with samples and the presence of AIV was determined using a hemagglutination assay after incubating the eggs at 37 o C for 45 days. AIVs were subtypedusing reverse transc ription-polymerase chain reaction and DNA sequencing. Results: Of the137,628 samples, we isolated total 650... -
Surveillance of Avian Influenza Viruses in South Korea between 2012 and 2014
Sang Hee Woo, Eun Kyoung Lee, Hyun Mi Kang, Byung Min Song, Gyeong Beum Heo, Joojin Jeong, Hyeon Jeong Kim, Suk Chan Jung, Youn Jeong Le 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 94 187-188 (2 pages)
or fecal samples from poultry farms and wild bird habitats. AIVs were isolated by embryonated SPF egg inoculation with samples and the presence of AIV was determined using a hemagglutination assay after incubating the eggs at 37 o C for 45 days. AIVs were subtypedusing reverse transc ription-polymerase chain reaction and DNA sequencing. Results: Of the137,628 samples, we isolated total 650... -
Isolation and characterization of a new variant porcine epidemic diarrhea virus occurred in Korea in 2014
Dong Kun Yang, Ha Hyun Kim, Seong Hun Lee, Soon Seek Yoon, Sung Suk Choi, Joong won Park, In Soo Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 37 196-197 (2 pages)
2), to understandtheir genomic backgrounds. Materials and Methods: 7viruses were propagated in 10-day-old specific-pathoge n-free (SPF) embryonated chickeneggs according to the standard procedure. Viral genomic RNA was extracted fromallantoic fluid and amplified with primer set by RT-PCR. The purified PCRamplicons were analyzed using an ABI3711 automatic sequencer and phylogenetictree was... -
Minimum Infectious Dose Determination of the K40/09Infectious bronchitis Virus vaccine candidate strain by spray administration
Jun-young Kim, Ha-na Youn, Woo-tack Hong, Hyo-sun Ju, Kyu-jik Kim, Seoung-su Yuk, Jung-hoon Kwon, Jin-yong Noh, Je-hyeon Jung, Sol Jeong, 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 157 296-296 (1 pages)
SP F embryonated chicken eggs via allantoic fluidcavity and harvested allantoic fluid is tested by HA assay using 1% chickenRBC. Results: In contrast topolymerase genes with C4 complete recombinant virus, r01310 was generated successfullyby using polymerase genes with U4. But, when PA plasmid was solely mutated intoU4, r01310 was not rescued. Conclusions: Changing weak(C4) to strong (U4)... -
Successful Generation of a Complete Recombinant H9N2 Avian Influenza Virus by modifying Hoffmann’s Reverse Genetics Vector System
Se Hee An, Chung Young Lee, Jun Goo Choi, Youn Jeong Lee, Jae Hong Kim, Hyuk Joon Kwon 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 156 295-296 (2 pages)
SP F embryonated chicken eggs via allantoic fluidcavity and harvested allantoic fluid is tested by HA assay using 1% chickenRBC. Results: In contrast topolymerase genes with C4 complete recombinant virus, r01310 was generated successfullyby using polymerase genes with U4. But, when PA plasmid was solely mutated intoU4, r01310 was not rescued. Conclusions: Changing weak(C4) to strong (U4)... -
Identification, sequence analysis, and infectivity of H9N2 avian influenza viruses isolated from geese
Rui Zhu, Xueqin Yang, Jianjun Zhang, Danwen Xu, Jiawen Fan, Huoying Shi, Shifeng Wang, Xiufan Liu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 10 406-415 (10 pages)
of the supernatant was inoculated into the allantoic cavities of 10-day-old SPF embryonated chicken eggs. After 72 h of incubation at 37 oC, the viruses were tested for hemagglutinin (HA) activity. The three isolated viruses were identified as subtype H9e by performing hemagglutination inhibition (HI) assays using antisera against subtype H9 AIV, subtype H5 AIV, and Newcastle disease virus... The subtype H9N2 avian influenza virus greatly threatens the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three H9N2 virus strains, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the... -
Experimental infection and pathology of clade 2.2 H5N1 virus in gulls
Marina A. Gulyaeva, Kirill A. Sharshov, Anna V. Zaykovskaia, Lidia V. Shestopalova, Aleksander M. Shestopalov 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 7 179-188 (10 pages)
on Customers’ Rights Protection and Human Well-being Surveillance, Koltsovo, Novosibirsk region, Russian Federation. Stocks of virus strain were prepared by the second passage in 9-day-old embryonated chicken eggs (ECE) using standard procedures [32,39]. Allantoic fluid from the infected eggs was diluted in phosphate-buffered saline to yield a final titer of 10 7 median egg infectious doses... During 2006, H5N1 HPAI caused an epizootic in wild birds, resulting in a die-off of Laridae in the Novosibirsk region at Chany Lake. In the present study, we infected common gulls (Larus canus) with a high dose of the H5N1 HPAI virus isolated from a common gull to determine if severe disease could be induced over the 28 day experimental period. Moderate clinical signs including diarrhea, conjunctivitis, respiratory distress and neurological signs were observed in virus-inoculated birds, and 50%... -
Prolonged excretion of a low-pathogenicity H5N2 avian influenza virus strain in the Pekin duck
José Manuel Carranza-Flores, Luis Padilla-Noriega, †, Elizabeth Loza-Rubio, Gary García-Espinosa 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 16 487-490 (4 pages)
at least 17 years in some Mexican chicken farms. We measured the rate and duration of viral excretion from Pekin ducks that were experimentally inoculated with an H5N2 LPAIV that causes death in embryonated chicken eggs (A/chicken/Mexico/2007). Leghorn chickens were used as susceptible host controls. The degree of viral excretion was evaluated with real-time reverse transcriptase-polymerase... were experimentally inoculated with an H5N2 LPAIV that causes death in embryonated chicken eggs (A/chicken/Mexico/2007). Leghorn chickens were used as susceptible host controls. The degree of viral excretion was evaluated with real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) using samples from oropharyngeal and cloacal swabs. We observed prolonged excretion from both species of birds lasting for at least 21 days. Prolonged excretion of LPAIV A/chicken/Mexico/2007 is atypical.