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Assessment of the safety and efficacy of low pathogenic avian influenza (H9N2) virus in inactivated oil emulsion vaccine in laying hens
Jeong-Hwa Shin, Jong Seo Mo, Jong-Nyeo Kim, In-pil Mo, Bong-Do Ha 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 4 27-34 (8 pages)
the original work is properly cited. pISSN 1229-845X eISSN 1976-555X Assessment of the safety and efficacy of low pathogenic avian influenza (H9N2) virus in inactivated oil emulsion vaccine in laying hens Jeong-Hwa Shin 1,3, Jong Seo Mo 2, Jong-Nyeo Kim 3, In-pil Mo 3, Bong-Do Ha 4,* 1 Environmental Health Research Division, National Institute of Environmental Research, Incheon 22689, Korea 2... In Korea, several outbreaks of low pathogenic AI (H9N2) viral infections leading to decreased egg production and increased mortality have been reported on commercial farms since 1996, resulting in severe economic losses. To control the H9N2 LPAI endemic, the Korea Veterinary Authority has permitted the use of the inactivated H9N2 LPAI vaccine since 2007. In this study, we developed a killed vaccine using a low pathogenic H9N2 AI virus (A/chicken/Korea/ADL0401) and conducted safety and efficacy... -
Increased humoral antibody response of foot-and-mouth disease virus vaccine in growing pigs pre-treated with poly-γ-glutamic acid
Jee-Hoon Lee, Ik-Jae Kang, A-Reum Kim, You-Sun Noh, Hee-Chun Chung, Bong-Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 15 253-256 (4 pages)
pivotal control tool against FMD virus infection, although this program is somewhat controversial. The FMD vaccine adapted in Korea consists of inactivated viruses with double oil-based emulsion (DOE) and includes structural proteins of FMD viruses (FMDVs) (O 1 Manisa + A Malaysia + Asia 1 Shamir serotypes). This typical combination effectively reduced FMD incidence during 2011, and has been... This study was conducted to determine if humoral antibody response of foot-and-mouth disease (FMD) vaccine improved in 8-week-old growing pigs born to well-vaccinated sows pre-treated with 60 mg of poly-γ-glutamic acid (γ-PGA) three days before vaccination. Antibody against FMD virus serotype O was measured 0, 2, 4 and 6 weeks post-vaccination, using a PrioCHECK FMDV type O ELISA kit. The results showed that positive antibody reactions against FMDV serotype O antigen among a component of the... -
Hematoporphyrin monomethyl ether combined with He?Ne laser irradiation-induced apoptosis in canine breast cancer cells through the mitochondrial pathway
Huatao Li, Jinjin Tong, Jun Bao, Damu Tang, Wenru Tian, Yun Liu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 13 235-242 (8 pages)
were washed three times with cold PBS, then mounted on glass slides and dipped into emulsion and glycerol/PBS (1 : 3, v/v) to measure their fluorescence. The cells were visualized and analyzed using a Ti-s fluorescence microscope (Leica, Germany). Cells with apoptosis in the group panel were quantified by randomly counting 200 positively stained cells. DNA fragmentation Samples (3 × 10 6... Hematoporphyrin monomethyl ether (HMME) combined with He-Ne laser irradiation is a novel and promising photodynamic therapy (PDT)-induced apoptosis that can be applied in vitro on canine breast cancer cells. However, the exact pathway responsible for HMME-PDT in canine breast cancer cells remains unknown. CHMm cells morphology and apoptosis were analyzed using optical microscope, terminal deoxynucleotidyl transferase dUTP nick end labeling fluorescein staining and DNA ladder assays. Apoptotic... -
Micro-computed tomography evaluation and pathological analyses of female rats with collagen-induced arthritis
Young Hee Kim, Jin Seok Kang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 5 165-171 (7 pages)
emulsion to induce arthritis except for the week 0 group. CIA was induced as follows. Bovine type II collagen (Chondrex, USA) was dissolved overnight in 0.01 M acetic acid at 4 oC. This was emulsified in an equal volume of incomplete Freund's adjuvant (Chondrex). The rats were immunized intradermally at the base of the tail with 0.1 mL of emulsion containing 100 of type II collagen. On day 7... Imaging techniques have been introduced to assess the efficacy and toxicity of developing pharmaceuticals. The purpose of this study was to perform a comprehensive characterization of collagen-induced arthritis (CIA) in rats using micro-computed tomography (micro-CT) and to compare the results with data from conventional pathological examination. Arthritis was induced by collagen in 24 female Wistar rats. Micro-CT and pathological analyses were performed to assess arthritis progression. Micro-CT... -
Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice
Jin-Joo Hue, Ki Nam Lee, Jae-Hwang Jeong, Sang-Hwa Lee, Young Ho Lee, Seong-woon Jeong, Sang Yoon Nam, Young Won Yun, Beom Jun L 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 3 189-195 (7 pages)
DG emulsion lowers the extent of increase in postprandial serum triglyceride (TG) levels in rats [38]. Dietary DG, in contrast to TG, decreases both body weight and visceral fat mass as determined by computed tomography in healthy men [29]. In addition, dietary DG suppresses the accumulation of high-fat and high-sucrose diet-induced body fat in C57BL/6J mice [28]. Conjugated linoleic acid... This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p ＜ 0.01). All... -
Increased expression of osteopontin in the spinal cords of Lewis rats with experimental autoimmune neuritis
Changjong Moon, Taekyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 2 289-293 (5 pages)
obtained from Harlan (Sprague Dawley, USA) and bred in our animal facility. Female rats, aged 7-12 weeks and weighing 160-200 g, were used. Each rat was injected in both hind feet with 200l of an emulsion containing equal parts of horse sciatic nerve in phosphate buffer (mg/ml) and complete Freund’s adjuvant (CFA; Mycobacterium tuberculosis H37Ra, 5 mg/ml; Difco, USA). *Corresponding author Tel:... To investigate the pattern of expression of osteopontin (OPN) in tissues of the central nervous system (CNS) responding to peripheral immunological stimulation, the expression of OPN was studied in the spinal cord of rats with experimental autoimmune neuritis (EAN). In this model system, the sciatic nerves and spinal nerve roots are the target organs of EAN and the spinal cord is a remote organ that may be indirectly affected. OPN was constitutively expressed in some astrocytes adjacent to the... -
Melatonin ameliorates autoimmune encephalomyelitis through suppression of intercellular adhesion molecule-1
JongChul Kang, Meejung Ahn, Yong-Sik Kim, Changjong Moon, Yongduk Lee, Myung-Bok Wie, Young-jae Lee and Taekyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 3 85-89 (5 pages)
inoculated subcutaneously in *Corresponding author Phone: +82-64-754-3363; Fax: +82-64-756-3354 E-mail: shint@cheju.cheju.ac.kr ..PAGE:2 86 JongChul Kang et al. both hind footpads with 100 ml of an emulsion containing 1 mg of fresh rat spinal cord homogenate in phosphate- buffered saline per ml of complete Freunds adjuvant (CFA; Mycobacterium tuberculosis H37Ra, Difco, Detroit, Michigan). Control... Melatonin (N-acetyl-5-methoxytryptamine), a pineal neurohormone, is a hydroxyl radical scavenger and antioxidant, and plays an important role in the immune system. We studied the effect of exogenous melatonin on the pathogenesis of experimental autoimmune encephalomyelitis (EAE). EAE was induced in Lewis rats by immunization with rat spinal cord homogenates. Subsequent oral administration of melatonin at 5 mg/kg significantly reduced the clinical severity of EAE paralysis compared with... -
Effects of dietary acetaminophen and vitamin C supplement on serum cortisol and tumor necrosis factor-alpha concentrations in pigs vaccinated with foot-and-mouth disease vaccine
Chun-nam Cha , Beom-jun Lee , Eun-kee Park , Chang-yeol Yoo , Suk Kim , Hu-jang Lee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 4 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 3호 8 197-200 (4 pages)
were started in September 2011 in the Republic of Korea using trivalent FMD vaccines (types O, A, and Asia1) [7]. The trivalent FMD vaccine consists of inacti- vated viruses with double oil-based emulsion and includes structural proteins of FMD viruses (O1 Manisa + A Malaysia + Asia 1 Shamir serotypes) [9]. Although multivalent FMD vaccines reduced the large FMD outbreak, a single-dose injection... -
Physiology,Pharmacology,Chemistry : Comparison of Linear and non-Linear equation for the calibration of roxithromycin analysis using Liquid chromatography/mass spectrometry
Jong Hwan Lim , Hyo In Yun 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2010, 제 50권 제 1호 2 11-17 (7 pages)
was followed by the method of Lim et al. [10, 11] with some modifications. Briefly, to 200L of spiked plasma, 10 L of 2 N NaOH and 800L of ethyl acetate were added. To prevent the formation of an emulsion, extraction was performed gently for 10 min on a test- tube rotator. After centrifugation, the organic phase was transferred to tube and evaporated at 30 oC under a stream of nitrogen and then... -
Potential of Using Ginger Essential Oils-Based Nanotechnology to Control Tropical Plant Diseases
Adamu Abdullahi, Khairulmazmi Ahmad, Intan Safinar Ismail, Norhayu Asib, Osumanu Haruna Ahmed, Abubakar Ismaila Abubakar, Yasmeen Siddiqui, Mohd Razi Ismail 한국식물병리학회 The Plant Pathology Journal 21 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 1 515-535 (21 pages)
nanoemulsions, mi- croemulsions, solid-lipid nanoparticles and liposomes are models for enclosure of such natural bioactive compounds to enhance antimicrobial activity (Pedro et al., 2013). Na- noemulsions are dispersions of nanoparticles consisting of two insoluble liquids, namely oil, and water, one of which is distributed by an emulsion-stabilizing surfactant on the other. Surfactants are... Essential oils (EOs) have gained a renewed interest in many disciplines such as plant disease control and medicine. This review discusses the components of ginger EOs, their mode of action, and their potential nanotechnology applications in controlling tropical plant diseases. Gas chromatography-mass spectroscopy (GC-MS), high-performance liquid chromatography, and headspace procedures are commonly used to detect and profile their chemical compositions EOs in ginger. The ginger EOs are composed...