자료유형
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Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development
Heechul Kim, Changjong Moon, Meejung Ahn, Yongduk Lee, Hwanglyong Kim, Seungjoon Kim, Taeyoung Ha, Youngheun Jee, Taekyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 2 97-101 (5 pages)
role that nNOS plays in the ovary remains to be determined. The findings, together with previous research, indicate that the expression of NOS is in part dependent on the stage of ovarian follicle development. At the early stage of follicular development, little NOS immunostaining was detected in granulosa or theca cells. In the later stages, including Graafian follicles, immunostaining for three... The expression of nitric oxide synthase (NOS) isoforms in the ovaries of pigs was examined to study the involvement of nitric oxide, a product of NOS activity, in the function of the ovary. Western blot analysis detected three types of NOS in the ovary, including constitutive neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS); eNOS immunoreactivity was more intense compared with that of iNOS or nNOS. Immunohistochemical studies demonstrated the presence of nNOS and eNOS in the... -
Effect of Concentration and Exposure Duration of FBS on Parthenogenetic Development of Porcine Follicular Oocytes
Kim. Hyun-Jong, Cho. Sang-Rae, Choe. Chang-Yong, Choi. Sun-Ho, Son. Dong-Soo, Kim. Sung-Jae, Sang. Byung-Don, Han. Man-Hye, Ryu. Il-Sun, 한국수정란이식학회 韓國受精卵移植學會誌 5 Pages
한국수정란이식학회 韓國受精卵移植學會誌 2007, Vol.22 No.4 245-249 (5 pages)
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3-Hyroxyflavone in Maturation Medium Supports In Vitro Development of Fertilized Bovine Follicular Oocytes
Kim. Se-Woong, Park. Jong-Im, Jung. Yeon-Gil, Roh. Sangho 한국동물번식학회 Reproductive & developmental biology 4 Pages
한국동물번식학회 Reproductive & developmental biology 2014, Vol.38 No.4 143-146 (4 pages)
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Effects of FBS(Fetal Bovine Serum) and pFF(Porcine Follicular Fluid) on In Vitro Maturation and Development of Porcine Parthenogenetic and Nuclear Transfer Embryos
Moon. Hyo-Jin, Shim. Joo-Hyun, Hwang. In-Sun, Park. Mi-Rung, Kim. Dong-Hoon, Ko. Yeoung-Gyu, Park. Choon-Keun, Im. Gi-Sun 한국동물번식학회 Reproductive & developmental biology 7 Pages
한국동물번식학회 Reproductive & developmental biology 2009, Vol.33 No.2 85-91 (7 pages)
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Relationship between follicular fluid adipocytokines and the quality of the oocyte and corresponding embryo development from a single dominant follicle in in vitro fertilization/intracytoplasmic sperm injection cycles
Chang. Hye Jin, Lee. Ji Hyun, Lee. Jung Ryeol, Jee. Byung Chul, Suh. Chang Suk, Kim. Seok Hyun 대한생식의학회 Clinical and experimental reproductive medicine 8 Pages
대한생식의학회 Clinical and experimental reproductive medicine 2014, Vol.41 No.1 21-28 (8 pages)
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Treatment of GDF8 on Oocyte Maturation activated SMAD2/3 signaling and improved subsequent invitro embryonic development
Jun-chul Yoon, Eun-song Lee, Sang Hwan Hyun 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 88 131-132 (2 pages)
of Technology(KIAT) through the Leading Industry Development for Economic Region (ProjectNo. R0004357)“ and “Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Advanced Production Technology Development Program, funded by Ministry of Agriculture, Food andRural Affairs (MAFRA) (Grant number: 115103-02)”, Republic of Korea.... -
Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer
Anukul Taweechaipaisankul, Geon A Kim, Jun-Xue Jin, Su Cheong Yeom, Byeong Chun Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 3 492-499 (8 pages)
3). As shown in the results, the CMAH expression level was similar to alpha-Gal expression in all cell types. In vitro embryo development after SCNT: kidney and skin fibroblast comparisons To examine the ability of donor cells used for SCNT to support in vitro development, cleavage and blastocyst formation rates were compared between kidney cells and skin fibroblasts derived from blood type... in embryo development. We found no significant bands corresponding to specific blood group A, and no increase in FUT expression in cell lines derived from piglets No. 1, No. 4, No. 5, No. 8, and the adult female KNP; moreover, they showed normal levels of expression of 1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase. There was no significant difference in embryo development between skin and kidney fibroblasts derived from the blood type O KNPs.... -
Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs
Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 16 373-380 (8 pages)
base medium for IVM was medium-199 (M-199; Invitrogen, USA), which consisted of 0.6 mM cysteine, 0.91 mM pyruvate, 10 ng/mL epidermal growth factor, 75 kanamycin, 1 g/mL insulin, and 10% (v/v) pig follicular fluid. IVM medium was supplemented with 80 g/mL FSH (Antrin R-10; Kyoritsu Seiyaku, Japan) and 10 IU/mL hCG (Intervet International BV, the Netherland) for the first 22 h of IVM. Porcin... This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Morphologically good (MGCOCs) and poor oocytes (MPCOCs) were untreated or treated with 1 nM rapamycin during 0–22 h, 22–42 h, or 0–42 h of IVM. Rapamycin had no significant effects on nuclear maturation and blastocyst formation... -
Development of in vitro produced porcine embryos according to serum types as macromolecule
Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 11 315-321 (7 pages)
a variety of sex steroids is contained in follicular fluid at concentrations signaificantly higher than that of serum [10,16,17]. However, while the importance of sex hormones is well known in the ovary, the influence of the follicular fluid on the oocyte development is not well understood. Changes in the steroid content of follicular fluid according to the stage of follicles (size, atretic... without gonadotrophin (GTH) and development rates of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were then compared. The maturation rates of the PGS group was significantly higher when GTH was not added. Additionally, during development of PA embryos without GTH, the PGS group showed significantly higher cleavage and blastocyst formation rates. Moreover, the cleavage rates of IVF embryos were significantly higher in the PGS group, with no significant differences in...